BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148819.6 - phase: 0
(156 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW585396 40 4e-04
AW696122 similar to GP|7208779|emb hypothetical protein {Cicer a... 35 0.012
TC86622 weakly similar to GP|20197623|gb|AAM15156.1 putative myo... 34 0.021
AW686152 weakly similar to PIR|T47645|T47 centromere protein-lik... 33 0.036
TC80825 similar to PIR|F84614|F84614 probable kinesin heavy chai... 32 0.080
TC82350 similar to GP|9837385|gb|AAG00554.1| retinitis pigmentos... 28 1.2
TC80581 similar to GP|19683021|gb|AAL92644.1 hypothetical protei... 28 2.0
TC83198 weakly similar to GP|13430502|gb|AAK25873.1 unknown prot... 28 2.0
TC86195 similar to GP|16974678|gb|AAL32439.1 SH3 domain-containi... 27 2.6
TC88259 similar to GP|6729037|gb|AAF27033.1| unknown protein {Ar... 27 3.4
TC86877 similar to GP|8953376|emb|CAB96649.1 putative protein {A... 27 4.4
TC80076 similar to GP|10177459|dbj|BAB10850. gene_id:MQB2.13~unk... 27 4.4
TC93156 similar to PIR|C96805|C96805 hypothetical protein T5M16.... 27 4.4
TC86215 similar to GP|19698867|gb|AAL91169.1 unknown protein {Ar... 26 7.5
TC86789 similar to PIR|A84594|A84594 hypothetical protein At2g20... 26 7.5
BQ752248 25 9.8
TC76450 similar to GP|17065130|gb|AAL32719.1 putative protein {A... 25 9.8
CA921757 homologue to SP|Q41784|TBB7 Tubulin beta-7 chain (Beta-... 25 9.8
>AW585396
Length = 555
Score = 40.0 bits (92), Expect = 4e-04
Identities = 34/134 (25%), Positives = 66/134 (48%), Gaps = 2/134 (1%)
Frame = +1
Query: 14 LLEEEYNEKIARLEAQVKESLHEKASYEATISQLHGDIAAHKNHMQILANRLDQVHFEVE 73
++EE N L++ V+E+ H S E + S L N ++ N +
Sbjct: 145 IIEETRNS----LDSSVEENAHLTTSLEKSQSLLKLKTKELNNLTNLMENSKRSL----- 297
Query: 74 SKYSSEIRDLKDCLMAEQEEKNDLNRKMQLLEKELLLFKAKMVDQQQ--EMTSNWQVETL 131
SK S EI DLKD + ++ N+L +++ + E K + +QQQ + ++ ++E +
Sbjct: 298 SKNSDEIADLKDDVKKGEKIINELRAELKASKVEAETAKKAVTEQQQHFDQSTKIKLEKI 477
Query: 132 KQKIMKLRKENEVL 145
+ +++KENE++
Sbjct: 478 VAEKEEMKKENEMI 519
>AW696122 similar to GP|7208779|emb hypothetical protein {Cicer arietinum},
partial (24%)
Length = 663
Score = 35.0 bits (79), Expect = 0.012
Identities = 38/144 (26%), Positives = 73/144 (50%), Gaps = 7/144 (4%)
Frame = +1
Query: 10 RALKLLEEEYNEKIARLEAQVKESLHEKASYEATISQLHGDIAAHKNHMQILANRLDQVH 69
+A K L +EYN++ A V+E E+A+ E + A + M + + +++++
Sbjct: 106 QAKKALRKEYNQEF---RAAVQE---ERAAREL--------LKAKRQEMDSVQSTMNRLN 243
Query: 70 FEVE-SKYSSEIRDLKDCLMAEQ---EEKNDLNRKMQLLEK---ELLLFKAKMVDQQQEM 122
+ S+IR+++ + E +E+ L R+++LL++ EL AK DQ Q +
Sbjct: 244 NAISVGDIDSKIRNMEHMIQHETLPLKEEKQLIRQIKLLKQNRGELSNIIAKQ-DQSQSL 420
Query: 123 TSNWQVETLKQKIMKLRKENEVLK 146
+E +++ LRKE +VLK
Sbjct: 421 DDKESMEEQTKRLQLLRKELDVLK 492
>TC86622 weakly similar to GP|20197623|gb|AAM15156.1 putative myosin heavy
chain {Arabidopsis thaliana}, partial (17%)
Length = 2546
Score = 34.3 bits (77), Expect = 0.021
Identities = 35/148 (23%), Positives = 70/148 (46%), Gaps = 5/148 (3%)
Frame = +2
Query: 12 LKLLEEEYNEKIARLEAQVKESLHEKASYEATISQLHG----DIAAHKNHMQILANRLDQ 67
L+++ ++ N +L++ + L E+ I + H ++A +++ + R
Sbjct: 254 LEIVRDDLNLTQVKLQS-TENDLKAAELRESEIREKHNAIEENLAVRGRDIELTSAR--- 421
Query: 68 VHFEVESKYSSEIRDLKDCLMAEQEEKNDLNRKMQ-LLEKELLLFKAKMVDQQQEMTSNW 126
+ E+ES + S RD + L E+ N + ++Q LLEK +L + +Q ++
Sbjct: 422 -NLELESLHESLTRDSEQKLQEAIEKFNSKDSEVQSLLEKIKILEENIAGAGEQSISLKS 598
Query: 127 QVETLKQKIMKLRKENEVLKRKFSHIEE 154
+ E K+ L+ ENE LKR+ E+
Sbjct: 599 EFEESLSKLASLQSENEDLKRQIVEAEK 682
>AW686152 weakly similar to PIR|T47645|T47 centromere protein-like -
Arabidopsis thaliana, partial (14%)
Length = 661
Score = 33.5 bits (75), Expect = 0.036
Identities = 28/130 (21%), Positives = 54/130 (41%), Gaps = 9/130 (6%)
Frame = +1
Query: 34 LHEKASYEATISQLHGDIAAHKNHMQILANRLDQVHFEVESKYSSEIRDLKDCLMAEQEE 93
L +K +E D+ ++ LA EV + S+I D
Sbjct: 10 LSKKLEHENFFPDYGYDVTTLIQTIRNLAEEKISYALEVSTHIRSQIADRASAKEELNRV 189
Query: 94 KNDLNRKMQLLEKELLLFKAKMVDQQQEMTSNW---------QVETLKQKIMKLRKENEV 144
K +L ++Q LEKE ++ + + +S+W + + L+++I +L ++N
Sbjct: 190 KTELEIRIQRLEKEKNEMQSALEKELDRRSSDWSFKLEKYQSEEQRLRERIRELAEQNVS 369
Query: 145 LKRKFSHIEE 154
L+R+ S E
Sbjct: 370 LQREVSSFSE 399
>TC80825 similar to PIR|F84614|F84614 probable kinesin heavy chain
[imported] - Arabidopsis thaliana, partial (16%)
Length = 1771
Score = 32.3 bits (72), Expect = 0.080
Identities = 28/140 (20%), Positives = 61/140 (43%), Gaps = 11/140 (7%)
Frame = +2
Query: 15 LEEEYNEKIARLEAQVKESLHEKASYEATISQLHGDIAAHKNHMQILANRLDQVHFEVES 74
+ + EKI LE Q++ + E +SQL + + L +++ ++ +++
Sbjct: 794 IHKNLQEKIKELEGQIELKTSMQNQSEKQVSQLCEKLKGKEETCCTLQHKVKELERKIKE 973
Query: 75 KYSSEI-----------RDLKDCLMAEQEEKNDLNRKMQLLEKELLLFKAKMVDQQQEMT 123
+ +E + LKD L + E + L K++ LE++L ++QE
Sbjct: 974 QLQTETANFQQKAWDLEKKLKDQLQGSESESSFLKDKIKELERKL---------KEQEQN 1126
Query: 124 SNWQVETLKQKIMKLRKENE 143
S ++ +++ + KE E
Sbjct: 1127SESLLKQ*MKELEEKHKERE 1186
>TC82350 similar to GP|9837385|gb|AAG00554.1| retinitis pigmentosa GTPase
regulator-like protein {Takifugu rubripes}, partial (2%)
Length = 759
Score = 28.5 bits (62), Expect = 1.2
Identities = 17/54 (31%), Positives = 26/54 (47%)
Frame = +1
Query: 8 HRRALKLLEEEYNEKIARLEAQVKESLHEKASYEATISQLHGDIAAHKNHMQIL 61
H+ A+ +L E + RLE + +S H T+ +HG I H+N M L
Sbjct: 592 HKMAISVLHE-----VVRLEPNLPDSYH-------TLGLVHGAIGDHENEMGFL 717
>TC80581 similar to GP|19683021|gb|AAL92644.1 hypothetical protein
{Dictyostelium discoideum}, partial (5%)
Length = 850
Score = 27.7 bits (60), Expect = 2.0
Identities = 16/54 (29%), Positives = 31/54 (56%), Gaps = 1/54 (1%)
Frame = +1
Query: 87 LMAEQEEKNDLNRKMQLLEKELLLFKAKMV-DQQQEMTSNWQVETLKQKIMKLR 139
LM +Q+ + QL +++LLL + ++V QQQ+ + + L+Q++ LR
Sbjct: 619 LMQQQKSMGQPLLQQQLQQQQLLLQQQQLVLQQQQQQQQQQRTQLLQQQMQALR 780
>TC83198 weakly similar to GP|13430502|gb|AAK25873.1 unknown protein
{Arabidopsis thaliana}, partial (2%)
Length = 609
Score = 27.7 bits (60), Expect = 2.0
Identities = 23/77 (29%), Positives = 38/77 (48%)
Frame = +2
Query: 78 SEIRDLKDCLMAEQEEKNDLNRKMQLLEKELLLFKAKMVDQQQEMTSNWQVETLKQKIMK 137
SE L+D L ++EE + KM L +K +A VD + L+QK+
Sbjct: 23 SETDGLRDALSLKEEEMEKM--KMGLAKKSE---EAAYVDSE-----------LRQKVQL 154
Query: 138 LRKENEVLKRKFSHIEE 154
L + NEV+K++ ++E
Sbjct: 155 LSEANEVVKKQEIELQE 205
>TC86195 similar to GP|16974678|gb|AAL32439.1 SH3 domain-containing protein
2 {Arabidopsis thaliana}, partial (58%)
Length = 848
Score = 27.3 bits (59), Expect = 2.6
Identities = 19/68 (27%), Positives = 35/68 (50%), Gaps = 1/68 (1%)
Frame = +2
Query: 9 RRALKLLEEEYNEKIA-RLEAQVKESLHEKASYEATISQLHGDIAAHKNHMQILANRLDQ 67
+R K+ E N +IA +LEA + E T+ +L + A +++ Q++ LDQ
Sbjct: 143 KRQAKVRELPGNSEIAMKLEAAAALAAVEAQQQRLTLQRLIAMVEAERSYHQVVLQILDQ 322
Query: 68 VHFEVESK 75
+ E+ S+
Sbjct: 323 LEGEMISE 346
>TC88259 similar to GP|6729037|gb|AAF27033.1| unknown protein {Arabidopsis
thaliana}, partial (35%)
Length = 1757
Score = 26.9 bits (58), Expect = 3.4
Identities = 23/73 (31%), Positives = 35/73 (47%), Gaps = 11/73 (15%)
Frame = +3
Query: 92 EEKNDLNRKMQLLEKELLLFKAK--MVDQQ----QEMTSNWQ-----VETLKQKIMKLRK 140
EE D+ L LL AK MV Q +E S W+ V +LKQ++ R+
Sbjct: 411 EEGIDVKTLTNELAAALLEISAKEDMVKQHSKVAEEAISGWEKAENEVSSLKQQLDAARQ 590
Query: 141 ENEVLKRKFSHIE 153
+N L+ + SH++
Sbjct: 591 KNSGLEDRVSHLD 629
>TC86877 similar to GP|8953376|emb|CAB96649.1 putative protein {Arabidopsis
thaliana}, partial (12%)
Length = 1558
Score = 26.6 bits (57), Expect = 4.4
Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 3/85 (3%)
Frame = +2
Query: 34 LHEKASYEATISQLHGDIAAHKN---HMQILANRLDQVHFEVESKYSSEIRDLKDCLMAE 90
++E A Y I + AA N M+ L L+ E+ ++ E+ D+K +
Sbjct: 1151 VNEIAEYIKLIDEHRSVEAAKANSDSEMESLRKELES-KVEILTQKEREVTDIKTKIEGI 1327
Query: 91 QEEKNDLNRKMQLLEKELLLFKAKM 115
+E +L K LEK L K+K+
Sbjct: 1328 RERLGELEMKSSDLEKNRLSIKSKV 1402
>TC80076 similar to GP|10177459|dbj|BAB10850. gene_id:MQB2.13~unknown
protein {Arabidopsis thaliana}, partial (21%)
Length = 910
Score = 26.6 bits (57), Expect = 4.4
Identities = 14/47 (29%), Positives = 26/47 (54%), Gaps = 1/47 (2%)
Frame = +2
Query: 97 LNRKMQLLEKELLLFKA-KMVDQQQEMTSNWQVETLKQKIMKLRKEN 142
+N + +L +L L K K+++QQQ WQ + K + +++K N
Sbjct: 758 MNNQRKLTTNQLWLKKPLKLLNQQQPQLQMWQKQAKKCWLQRVKKGN 898
>TC93156 similar to PIR|C96805|C96805 hypothetical protein T5M16.17
[imported] - Arabidopsis thaliana, partial (8%)
Length = 593
Score = 26.6 bits (57), Expect = 4.4
Identities = 22/75 (29%), Positives = 37/75 (49%), Gaps = 11/75 (14%)
Frame = +2
Query: 89 AEQEEKNDLNRKMQLLEKELLLFKAK--MVDQQ----QEMTSNWQ-----VETLKQKIMK 137
+ +E D++ L LL AK +V Q +E S W+ V TLKQ++
Sbjct: 305 SNEEVVTDVHTLTGKLSAALLDISAKEDLVKQNAKVAEEAVSGWEKAENEVLTLKQQLDA 484
Query: 138 LRKENEVLKRKFSHI 152
+++N VL+ + SH+
Sbjct: 485 AKQQNAVLEDQVSHL 529
>TC86215 similar to GP|19698867|gb|AAL91169.1 unknown protein {Arabidopsis
thaliana}, partial (91%)
Length = 2293
Score = 25.8 bits (55), Expect = 7.5
Identities = 12/34 (35%), Positives = 20/34 (58%), Gaps = 3/34 (8%)
Frame = -2
Query: 43 TISQLHGDIAAHKNHMQI---LANRLDQVHFEVE 73
TI Q+HGD +H N Q+ L++R +H ++
Sbjct: 1092 TILQVHGDATSHPNQHQMIQKLSSRNQLLHVSIQ 991
>TC86789 similar to PIR|A84594|A84594 hypothetical protein At2g20840
[imported] - Arabidopsis thaliana, partial (93%)
Length = 1421
Score = 25.8 bits (55), Expect = 7.5
Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -3
Query: 23 IARLEAQVKESLH-EKASYEATISQLHGDIAAHKNHMQILANRLDQVHFE 71
I LE +V L SY QLH D AH+ +++L RL + HF+
Sbjct: 441 IQTLELEVPSPLL*HPLSYREGCQQLHYDHKAHEEGVRVL--RLQKEHFQ 298
>BQ752248
Length = 882
Score = 25.4 bits (54), Expect = 9.8
Identities = 13/28 (46%), Positives = 14/28 (49%)
Frame = +1
Query: 112 KAKMVDQQQEMTSNWQVETLKQKIMKLR 139
K K EMT WQ E +QK MK R
Sbjct: 370 KDKQPSTPTEMTDGWQNEDQQQKHMKRR 453
>TC76450 similar to GP|17065130|gb|AAL32719.1 putative protein {Arabidopsis
thaliana}, partial (83%)
Length = 2171
Score = 25.4 bits (54), Expect = 9.8
Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
Frame = -1
Query: 84 KDCLMAEQEEKNDL-------NRKMQLLEKELLLFKAKMVDQQQEMTSNWQVETLKQKIM 136
K ++ +Q EK ++ N ++ LEK LLLF D++ N+Q + K+
Sbjct: 245 KTDMITDQREKGNMKLCGEAVNLGLEKLEKILLLFHFHGKDRKMNE*KNFQRREERVKVG 66
Query: 137 KLRKENEVLKRKFSH 151
L ++ + + K H
Sbjct: 65 PLEEKEKKCQTKQPH 21
>CA921757 homologue to SP|Q41784|TBB7 Tubulin beta-7 chain (Beta-7 tubulin).
[Maize] {Zea mays}, partial (34%)
Length = 743
Score = 25.4 bits (54), Expect = 9.8
Identities = 11/23 (47%), Positives = 18/23 (77%)
Frame = -2
Query: 123 TSNWQVETLKQKIMKLRKENEVL 145
TSN +++ L +KIM+ RK+ +VL
Sbjct: 331 TSNTRMQLLTKKIMRRRKKRKVL 263
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.313 0.127 0.334
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,532,943
Number of Sequences: 36976
Number of extensions: 34241
Number of successful extensions: 165
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of query: 156
length of database: 9,014,727
effective HSP length: 88
effective length of query: 68
effective length of database: 5,760,839
effective search space: 391737052
effective search space used: 391737052
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC148819.6