BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148819.5 - phase: 0
(83 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF635063 weakly similar to PIR|F84486|F84 probable retroelement ... 108 5e-25
BG646342 weakly similar to PIR|F84486|F84 probable retroelement ... 33 0.015
BG587684 similar to GP|12858190|dbj evidence:NAS~putative~unclas... 31 0.097
TC87700 weakly similar to PIR|S31196|S31196 hypothetical protein... 28 0.63
TC82402 26 3.1
BQ156883 similar to GP|19699365|gb| At2g34960/F19I3.19 {Arabidop... 26 3.1
TC92303 similar to GP|21592565|gb|AAM64514.1 latex-abundant prot... 25 5.3
TC80386 weakly similar to GP|10800070|dbj|BAB16490. contains EST... 25 7.0
BQ751923 similar to SP|P38681|NIR Nitrite reductase [NAD(P)H] (E... 24 9.1
BI308419 similar to GP|4140398|gb| sterol-C5(6)-desaturase {Nico... 24 9.1
AW775288 similar to GP|4140398|gb| sterol-C5(6)-desaturase {Nico... 24 9.1
>BF635063 weakly similar to PIR|F84486|F84 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(4%)
Length = 677
Score = 108 bits (269), Expect = 5e-25
Identities = 54/83 (65%), Positives = 63/83 (75%)
Frame = -2
Query: 1 MGTKWDIEKFIGDSDFGLWKVKIEVVLIQQKCAKARKGKILFSDTMSQEDKTKMADNARS 60
MG+K DIEKF GD+DFGLWKVK+E VLIQQKC KA KG++ TMS+ +KT+M D ARS
Sbjct: 568 MGSKRDIEKFTGDNDFGLWKVKMEAVLIQQKCEKALKGEVSLPVTMSRAEKTEMVDKARS 389
Query: 61 FFVLCLRDKVLREVVKDTTIKVM 83
VLCL DKVLREV K+ T M
Sbjct: 388 AIVLCLGDKVLREVAKERTAASM 320
>BG646342 weakly similar to PIR|F84486|F84 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(4%)
Length = 599
Score = 33.5 bits (75), Expect = 0.015
Identities = 17/26 (65%), Positives = 18/26 (68%)
Frame = +2
Query: 58 ARSFFVLCLRDKVLREVVKDTTIKVM 83
ARS VLCL DKVLREV K+ T M
Sbjct: 2 ARSAIVLCLGDKVLREVAKEPTATSM 79
>BG587684 similar to GP|12858190|dbj evidence:NAS~putative~unclassifiable
{Mus musculus}, partial (14%)
Length = 819
Score = 30.8 bits (68), Expect = 0.097
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +3
Query: 1 MGTKWDIEKFIGDSDF 16
M TKWDIE FIG++DF
Sbjct: 735 MDTKWDIEIFIGENDF 782
>TC87700 weakly similar to PIR|S31196|S31196 hypothetical protein - potato,
partial (47%)
Length = 791
Score = 28.1 bits (61), Expect = 0.63
Identities = 10/18 (55%), Positives = 15/18 (82%)
Frame = -1
Query: 10 FIGDSDFGLWKVKIEVVL 27
+I SDFGLWKV++ ++L
Sbjct: 386 YIN*SDFGLWKVRLNILL 333
>TC82402
Length = 754
Score = 25.8 bits (55), Expect = 3.1
Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 3/57 (5%)
Frame = +1
Query: 11 IGDSDFGLWKVKIEVVLIQQK---CAKARKGKILFSDTMSQEDKTKMADNARSFFVL 64
I D++ + K +IE+V ++++ C K + G +++ KTK RSF L
Sbjct: 361 IVDANLNVLKERIEMVKVKERFERCCKCQHGWNYVPLSINDHTKTKRDKELRSFIQL 531
>BQ156883 similar to GP|19699365|gb| At2g34960/F19I3.19 {Arabidopsis
thaliana}, partial (19%)
Length = 573
Score = 25.8 bits (55), Expect = 3.1
Identities = 8/15 (53%), Positives = 13/15 (86%)
Frame = -3
Query: 13 DSDFGLWKVKIEVVL 27
+S+ GLWK+KI ++L
Sbjct: 490 ESEIGLWKIKITIIL 446
>TC92303 similar to GP|21592565|gb|AAM64514.1 latex-abundant protein
putative {Arabidopsis thaliana}, partial (4%)
Length = 687
Score = 25.0 bits (53), Expect = 5.3
Identities = 13/34 (38%), Positives = 22/34 (64%), Gaps = 3/34 (8%)
Frame = +3
Query: 25 VVLIQQKCAKARKGKIL---FSDTMSQEDKTKMA 55
V+L+ CAK ++GKIL F +++ KT++A
Sbjct: 465 VILLLLHCAKKKEGKILICNFERVSNEKLKTELA 566
>TC80386 weakly similar to GP|10800070|dbj|BAB16490. contains ESTs
C61063(AU063369) C61063(AU093310)~unknown protein,
partial (17%)
Length = 655
Score = 24.6 bits (52), Expect = 7.0
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +1
Query: 50 DKTKMADNARSFFVLCLRDKVLREV 74
+KT SFFV CL++ +LRE+
Sbjct: 253 NKTTFFLTTLSFFVSCLQNHILREL 327
>BQ751923 similar to SP|P38681|NIR Nitrite reductase [NAD(P)H] (EC 1.6.6.4).
{Neurospora crassa}, partial (9%)
Length = 667
Score = 24.3 bits (51), Expect = 9.1
Identities = 11/48 (22%), Positives = 23/48 (47%)
Frame = -3
Query: 12 GDSDFGLWKVKIEVVLIQQKCAKARKGKILFSDTMSQEDKTKMADNAR 59
GD+ +W+++ QQ C ++ +L + +E T D+A+
Sbjct: 623 GDTQLAVWRIRGRYYATQQMCPH-KRAFVLSDGLIGEEVGTDTCDDAK 483
>BI308419 similar to GP|4140398|gb| sterol-C5(6)-desaturase {Nicotiana
tabacum}, partial (92%)
Length = 804
Score = 24.3 bits (51), Expect = 9.1
Identities = 10/41 (24%), Positives = 19/41 (45%)
Frame = -3
Query: 36 RKGKILFSDTMSQEDKTKMADNARSFFVLCLRDKVLREVVK 76
+ G ++ + QED+ R +FV C D + ++ K
Sbjct: 619 KPGYVVMLEVFHQEDEKPNQQMGREYFVYCRYDVLHEDICK 497
>AW775288 similar to GP|4140398|gb| sterol-C5(6)-desaturase {Nicotiana
tabacum}, partial (73%)
Length = 716
Score = 24.3 bits (51), Expect = 9.1
Identities = 10/41 (24%), Positives = 19/41 (45%)
Frame = -1
Query: 36 RKGKILFSDTMSQEDKTKMADNARSFFVLCLRDKVLREVVK 76
+ G ++ + QED+ R +FV C D + ++ K
Sbjct: 674 KSGYVVMLEVFHQEDEKPNQQMGREYFVYCRCDVLHEDICK 552
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.137 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,303,809
Number of Sequences: 36976
Number of extensions: 20529
Number of successful extensions: 134
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of query: 83
length of database: 9,014,727
effective HSP length: 59
effective length of query: 24
effective length of database: 6,833,143
effective search space: 163995432
effective search space used: 163995432
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 51 (24.3 bits)
Medicago: description of AC148819.5