BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148755.4 + phase: 1 /partial
(441 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ147391 weakly similar to PIR|C96516|C96 F16N3.15 [imported] - ... 350 5e-97
TC89902 similar to PIR|C96516|C96516 F16N3.15 [imported] - Arabi... 256 6e-69
AW688516 PIR|C96516|C965 F16N3.15 [imported] - Arabidopsis thali... 235 3e-63
TC93528 similar to GP|20466490|gb|AAM20562.1 unknown protein {Ar... 40 0.001
TC79652 weakly similar to GP|19698951|gb|AAL91211.1 unknown prot... 40 0.002
TC78227 similar to GP|10176883|dbj|BAB10113. contains similarity... 39 0.003
TC88686 MTD2 39 0.003
TC89939 similar to GP|15289911|dbj|BAB63606. hypothetical protei... 39 0.004
BG451708 39 0.005
TC92850 similar to GP|15529216|gb|AAK97702.1 At1g72220/T9N14_22 ... 38 0.007
TC90157 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1... 37 0.012
TC82570 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1... 37 0.012
TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16... 37 0.012
TC91938 similar to PIR|E96612|E96612 probable transcription fact... 37 0.020
AJ497196 weakly similar to SP|O60683|PEXA_ Peroxisome assembly p... 36 0.026
TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2... 36 0.034
TC79248 similar to GP|22165059|gb|AAM93676.1 unknown protein {Or... 36 0.034
TC89100 similar to GP|21553849|gb|AAM62942.1 putative RING zinc ... 36 0.034
TC84456 similar to GP|15450836|gb|AAK96689.1 Unknown protein {Ar... 36 0.034
TC79053 similar to GP|22795037|gb|AAN05420.1 putative RING prote... 36 0.034
>BQ147391 weakly similar to PIR|C96516|C96 F16N3.15 [imported] - Arabidopsis
thaliana, partial (34%)
Length = 532
Score = 350 bits (899), Expect = 5e-97
Identities = 163/164 (99%), Positives = 163/164 (99%)
Frame = +2
Query: 278 RPFCGAYWSALGVTGSGSYPVCSPDTLRPISEHTISGIPLVAHEKNLHEQNITESCIRQM 337
RPFCGAYWSALGVTGSGSYPVCSPDTLRPISEHTISGIPLVAHEKNLHEQNITESCIRQM
Sbjct: 2 RPFCGAYWSALGVTGSGSYPVCSPDTLRPISEHTISGIPLVAHEKNLHEQNITESCIRQM 181
Query: 338 GRTLQDVISEWIAKLNNREIDTTRLMLNHAEMMTAGTLVCSDCYQKLVSFFLYWFRISTP 397
GRTLQDVISEWIAKLNNREIDTTRLMLNHAEMMTAGTLVCSDCY KLVSFFLYWFRISTP
Sbjct: 182 GRTLQDVISEWIAKLNNREIDTTRLMLNHAEMMTAGTLVCSDCYXKLVSFFLYWFRISTP 361
Query: 398 KHLLPPEASTREDCWYGYACRTQHRSEEHARKRNHVCRPTRGSH 441
KHLLPPEASTREDCWYGYACRTQHRSEEHARKRNHVCRPTRGSH
Sbjct: 362 KHLLPPEASTREDCWYGYACRTQHRSEEHARKRNHVCRPTRGSH 493
>TC89902 similar to PIR|C96516|C96516 F16N3.15 [imported] - Arabidopsis
thaliana, partial (6%)
Length = 660
Score = 256 bits (653), Expect(2) = 6e-69
Identities = 124/124 (100%), Positives = 124/124 (100%)
Frame = +3
Query: 1 QSDSRYPDVEIRSDKGQIRSEISTASCDKHRWCKIVRNSDLCSATLKNKSSNTILVDGAE 60
QSDSRYPDVEIRSDKGQIRSEISTASCDKHRWCKIVRNSDLCSATLKNKSSNTILVDGAE
Sbjct: 264 QSDSRYPDVEIRSDKGQIRSEISTASCDKHRWCKIVRNSDLCSATLKNKSSNTILVDGAE 443
Query: 61 VGKGDTVVIKDRSEIIPGPAKEGFVSYKFQIVSSPEICQTLLKICVDVDHAKCSICLNIW 120
VGKGDTVVIKDRSEIIPGPAKEGFVSYKFQIVSSPEICQTLLKICVDVDHAKCSICLNIW
Sbjct: 444 VGKGDTVVIKDRSEIIPGPAKEGFVSYKFQIVSSPEICQTLLKICVDVDHAKCSICLNIW 623
Query: 121 HDVV 124
HDVV
Sbjct: 624 HDVV 635
Score = 23.5 bits (49), Expect(2) = 6e-69
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = +1
Query: 123 VVTVAPCLHN 132
++TVAPCLHN
Sbjct: 631 LLTVAPCLHN 660
>AW688516 PIR|C96516|C965 F16N3.15 [imported] - Arabidopsis thaliana, partial
(1%)
Length = 591
Score = 235 bits (600), Expect(2) = 3e-63
Identities = 116/117 (99%), Positives = 116/117 (99%)
Frame = +2
Query: 1 QSDSRYPDVEIRSDKGQIRSEISTASCDKHRWCKIVRNSDLCSATLKNKSSNTILVDGAE 60
QSDSRYPDVEIRSDKGQIRSEISTASCDKHRWCKIVRNSDLCSATLKNKSSNTILVDGAE
Sbjct: 221 QSDSRYPDVEIRSDKGQIRSEISTASCDKHRWCKIVRNSDLCSATLKNKSSNTILVDGAE 400
Query: 61 VGKGDTVVIKDRSEIIPGPAKEGFVSYKFQIVSSPEICQTLLKICVDVDHAKCSICL 117
VGKGDTVVIKDRSEIIPGPAKEGFVSYKFQIVSSPEICQTLLKICVDVDHAKCSI L
Sbjct: 401 VGKGDTVVIKDRSEIIPGPAKEGFVSYKFQIVSSPEICQTLLKICVDVDHAKCSILL 571
Score = 24.6 bits (52), Expect(2) = 3e-63
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3
Query: 116 CLNIWHDV 123
CLNIWHDV
Sbjct: 567 CLNIWHDV 590
>TC93528 similar to GP|20466490|gb|AAM20562.1 unknown protein {Arabidopsis
thaliana}, partial (24%)
Length = 755
Score = 40.4 bits (93), Expect = 0.001
Identities = 19/50 (38%), Positives = 25/50 (50%)
Frame = +3
Query: 113 CSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQ 162
C+IC H + + C H FC C SEW R + CP CRA+V+
Sbjct: 168 CAICQEKMHSPILLR-CKHIFCEDCVSEWFERER------TCPLCRALVK 296
>TC79652 weakly similar to GP|19698951|gb|AAL91211.1 unknown protein
{Arabidopsis thaliana}, partial (20%)
Length = 1590
Score = 40.0 bits (92), Expect = 0.002
Identities = 27/80 (33%), Positives = 35/80 (43%)
Frame = +2
Query: 80 AKEGFVSYKFQIVSSPEICQTLLKICVDVDHAKCSICLNIWHDVVTVAPCLHNFCNGCFS 139
A+ F K Q S E + ++ D KCSIC + +VV + C H FCN C
Sbjct: 656 ARRNFSHLKAQDEDSSETDKLQQELGEYRDIVKCSICRDRTKEVV-ITKCYHLFCNSCIQ 832
Query: 140 EWLRRSQEKRSTVLCPQCRA 159
+ Q K CPQC A
Sbjct: 833 KIAGSRQRK-----CPQCGA 877
>TC78227 similar to GP|10176883|dbj|BAB10113. contains similarity to unknown
protein~gb|AAD10691.1~gene_id:K9L2.3 {Arabidopsis
thaliana}, partial (27%)
Length = 1707
Score = 39.3 bits (90), Expect = 0.003
Identities = 18/47 (38%), Positives = 22/47 (46%)
Frame = +1
Query: 112 KCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCR 158
+C ICL I TV CLH FC C + +R + CP CR
Sbjct: 322 QCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNE-----CPACR 447
>TC88686 MTD2
Length = 934
Score = 39.3 bits (90), Expect = 0.003
Identities = 20/54 (37%), Positives = 27/54 (49%), Gaps = 1/54 (1%)
Frame = +1
Query: 106 VDVDHAK-CSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCR 158
+D++ K C +CL + VV + C H C C+ EW RSQ CP CR
Sbjct: 457 IDIEREKECGVCLEVKAKVV-LPNCCHQMCFKCYREWCLRSQS------CPFCR 597
>TC89939 similar to GP|15289911|dbj|BAB63606. hypothetical protein~similar
to Arabidopsis thaliana chromosome 1 F18O14.3, partial
(23%)
Length = 978
Score = 38.9 bits (89), Expect = 0.004
Identities = 17/56 (30%), Positives = 30/56 (53%)
Frame = +2
Query: 107 DVDHAKCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQ 162
D + +C+IC ++ D + + C H FC C +WL + R CP C+A+++
Sbjct: 128 DAGNFECNICFDLAQDPI-ITLCGHLFCWPCLYKWLHFHSQSRE---CPVCKAMIE 283
>BG451708
Length = 608
Score = 38.5 bits (88), Expect = 0.005
Identities = 23/100 (23%), Positives = 40/100 (40%), Gaps = 6/100 (6%)
Frame = +2
Query: 97 ICQTLLKICVDVDHA-KCSICLNIWHDVVTVA-----PCLHNFCNGCFSEWLRRSQEKRS 150
+C TL + D +C +CL+ D+ PC H+FC C ++ K
Sbjct: 53 LCVTLASEMEEDDSMWECPVCLDKMEDIGFPISSIDDPCTHSFCIPCSHSLSESARLKSQ 232
Query: 151 TVLCPQCRAVVQFVGKNHFLRTIAEDMVKADSSLRRSHDE 190
+ CP CR ++ N + ++ K L + D+
Sbjct: 233 NLQCPVCRRIIHGFAHNQIASVMMRELDKCKYLLGEAKDK 352
>TC92850 similar to GP|15529216|gb|AAK97702.1 At1g72220/T9N14_22
{Arabidopsis thaliana}, partial (19%)
Length = 687
Score = 38.1 bits (87), Expect = 0.007
Identities = 20/55 (36%), Positives = 27/55 (48%), Gaps = 3/55 (5%)
Frame = +3
Query: 108 VDHAKCSICLNIWHDVVT---VAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRA 159
++ +CS+CLN +H+ T + C H F C WL RS CP CRA
Sbjct: 468 IEGTECSVCLNEFHEDETLRLLPKCSHAFHISCIDTWL------RSHTNCPLCRA 614
>TC90157 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1a
[imported] - Arabidopsis thaliana (fragment), partial
(44%)
Length = 1486
Score = 37.4 bits (85), Expect = 0.012
Identities = 27/75 (36%), Positives = 37/75 (49%), Gaps = 2/75 (2%)
Frame = +1
Query: 113 CSICLNIW--HDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQFVGKNHFL 170
CSICL + HD TV C H + C EW +RS+E CP C + K+
Sbjct: 184 CSICLESFSVHDPSTVTCCKHEYHLHCILEWSQRSKE------CPICWQSLSL--KDPAC 339
Query: 171 RTIAEDMVKADSSLR 185
+ + D V+A+ SLR
Sbjct: 340 QELL-DAVEAEKSLR 381
>TC82570 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1a
[imported] - Arabidopsis thaliana (fragment), partial
(23%)
Length = 738
Score = 37.4 bits (85), Expect = 0.012
Identities = 27/75 (36%), Positives = 37/75 (49%), Gaps = 2/75 (2%)
Frame = +2
Query: 113 CSICLNIW--HDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQFVGKNHFL 170
CSICL + HD TV C H + C EW +RS+E CP C + K+
Sbjct: 212 CSICLESFSVHDPSTVTCCKHEYHLHCILEWSQRSKE------CPICWQSLSL--KDPAC 367
Query: 171 RTIAEDMVKADSSLR 185
+ + D V+A+ SLR
Sbjct: 368 QELL-DAVEAEKSLR 409
>TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16.190 -
Arabidopsis thaliana, partial (81%)
Length = 1378
Score = 37.4 bits (85), Expect = 0.012
Identities = 19/52 (36%), Positives = 24/52 (45%), Gaps = 2/52 (3%)
Frame = +2
Query: 109 DHAKCSICLNIWH--DVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCR 158
D CS+CL + DV+ PCLH F C WLR+ CP C+
Sbjct: 866 DELTCSVCLEQVNVGDVLRSLPCLHQFHASCIDPWLRQQG------TCPVCK 1003
>TC91938 similar to PIR|E96612|E96612 probable transcription factor
F12K22.14 [imported] - Arabidopsis thaliana, partial
(11%)
Length = 685
Score = 36.6 bits (83), Expect = 0.020
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = +3
Query: 113 CSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVV 161
CS C+ + VT PC HNFC CF +W+ + C CR +
Sbjct: 447 CSFCVKLPERPVTT-PCGHNFCLKCFEKWVGLGKR-----TCSNCRTAI 575
>AJ497196 weakly similar to SP|O60683|PEXA_ Peroxisome assembly protein 10
(Peroxin-10). [Human] {Homo sapiens}, partial (11%)
Length = 582
Score = 36.2 bits (82), Expect = 0.026
Identities = 19/51 (37%), Positives = 24/51 (46%)
Frame = +1
Query: 112 KCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQ 162
+CS+CL T PC H FC C S WL+ + CP CR V+
Sbjct: 307 RCSLCLEA-RTQDTATPCGHVFCWTCISGWLQTRAQ------CPLCRDFVE 438
>TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
[imported] - Arabidopsis thaliana, partial (69%)
Length = 1554
Score = 35.8 bits (81), Expect = 0.034
Identities = 20/47 (42%), Positives = 22/47 (46%), Gaps = 2/47 (4%)
Frame = +3
Query: 113 CSICLN--IWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQC 157
CSICL I D TV C H F C EW +RS + CP C
Sbjct: 198 CSICLEEFIDSDPSTVTSCKHEFHLQCILEWCQRSSQ------CPMC 320
>TC79248 similar to GP|22165059|gb|AAM93676.1 unknown protein {Oryza sativa
(japonica cultivar-group)}, partial (97%)
Length = 1272
Score = 35.8 bits (81), Expect = 0.034
Identities = 21/77 (27%), Positives = 37/77 (47%), Gaps = 1/77 (1%)
Frame = +2
Query: 112 KCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQFVGKNH-FL 170
+C IC+ + + + + C H C C+ EW RSQ CP CR ++ V ++
Sbjct: 557 ECGICMEM-NSKIVLPNCNHVMCLKCYHEWRARSQS------CPFCRDSLKRVNSGDLWI 715
Query: 171 RTIAEDMVKADSSLRRS 187
T + D+V ++ R +
Sbjct: 716 FTDSRDIVDMETVTREN 766
>TC89100 similar to GP|21553849|gb|AAM62942.1 putative RING zinc finger
protein {Arabidopsis thaliana}, partial (44%)
Length = 1047
Score = 35.8 bits (81), Expect = 0.034
Identities = 19/56 (33%), Positives = 27/56 (47%)
Frame = +1
Query: 107 DVDHAKCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQ 162
DV +C+IC ++ D V + C H FC C WL + P C+A+VQ
Sbjct: 343 DVGDFECNICFDLAQDPV-ITLCGHLFCWPCLYRWLHHHSHSQE---FPVCKALVQ 498
>TC84456 similar to GP|15450836|gb|AAK96689.1 Unknown protein {Arabidopsis
thaliana}, partial (6%)
Length = 809
Score = 35.8 bits (81), Expect = 0.034
Identities = 16/51 (31%), Positives = 23/51 (44%), Gaps = 2/51 (3%)
Frame = +2
Query: 113 CSICLN--IWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVV 161
C++CL + D + PCLH F C WL R CP C++ +
Sbjct: 41 CAVCLETPVKGDTIRHLPCLHKFHKDCIDPWLGRKSS------CPVCKSSI 175
>TC79053 similar to GP|22795037|gb|AAN05420.1 putative RING protein {Populus
x canescens}, partial (28%)
Length = 1139
Score = 35.8 bits (81), Expect = 0.034
Identities = 20/52 (38%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +2
Query: 113 CSICLNIWHDVVTVAPCLHNFCNGCFSEWLR---RSQEKRSTVLCPQCRAVV 161
C+ICL +D V V C H +C C +WL S E + CP C+AV+
Sbjct: 254 CNICLESANDPV-VTLCGHLYCWPCIYKWLNVQSSSVEPDTQPTCPVCKAVI 406
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.134 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,237,300
Number of Sequences: 36976
Number of extensions: 305237
Number of successful extensions: 1613
Number of sequences better than 10.0: 97
Number of HSP's better than 10.0 without gapping: 1596
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1611
length of query: 441
length of database: 9,014,727
effective HSP length: 99
effective length of query: 342
effective length of database: 5,354,103
effective search space: 1831103226
effective search space used: 1831103226
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC148755.4