BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148653.2 + phase: 0
(117 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86653 weakly similar to GP|21592732|gb|AAM64681.1 cysteine pro... 127 8e-31
BQ148646 weakly similar to GP|11596186|gb| cystatin-like protein... 114 5e-27
TC86465 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhi... 69 2e-13
TC77929 similar to PIR|T07139|T07139 cysteine proteinase inhibit... 66 2e-12
TC77459 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhi... 61 5e-11
TC89955 similar to GP|22901842|gb|AAN10097.1 NADH dehydrogenase ... 56 2e-09
TC87034 homologue to SP|P13983|EXTN_TOBAC Extensin precursor (Ce... 50 1e-07
BI310700 weakly similar to GP|21592712|gb| cystatin-like protein... 49 2e-07
BI311369 similar to GP|11596186|gb| cystatin-like protein {Citru... 47 1e-06
AJ502739 similar to GP|10119914|gb brain otoferlin {Mus musculus... 30 0.14
TC77500 weakly similar to SP|P21747|EA92_VICFA Embryonic abundan... 29 0.30
TC76471 similar to PIR|T51392|T51392 probable phytochelatin synt... 28 0.39
TC89627 weakly similar to GP|17529290|gb|AAL38872.1 putative pec... 27 0.88
BQ144829 25 3.3
BF642440 similar to GP|4874298|gb| expressed protein {Arabidopsi... 25 4.4
TC85222 homologue to SP|P34922|G3PC_PEA Glyceraldehyde 3-phospha... 25 4.4
TC79565 similar to SP|Q9ZQ70|WRK3_ARATH Probable WRKY transcript... 25 4.4
BE325280 similar to GP|14334426|gb unknown protein {Arabidopsis ... 25 4.4
TC93880 similar to GP|19310478|gb|AAL84973.1 AT3g05350/T12H1_32 ... 25 4.4
BI312045 similar to GP|22324956|gb| putative disease resistant p... 25 5.7
>TC86653 weakly similar to GP|21592732|gb|AAM64681.1 cysteine proteinase
inhibitor like protein {Arabidopsis thaliana}, partial
(64%)
Length = 651
Score = 127 bits (318), Expect = 8e-31
Identities = 62/113 (54%), Positives = 82/113 (71%)
Frame = +2
Query: 3 NPTLLLVLLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKLK 62
N + ++ L VV +F+ + ++ GGW+PI ++ DP V EIA FAVTEY ++SG+KL
Sbjct: 173 NVAIFVLFLSVVSVFTVSDGKDALV-GGWTPIKNVKDPQVVEIAKFAVTEYGKQSGSKLS 349
Query: 63 FEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSFVPV 115
F KVI GE+QVV+GTNYRL L+A S +KNYEA+VWEK W H +NLTSF PV
Sbjct: 350 FVKVIKGETQVVSGTNYRLVLAAKVVSATKNYEALVWEKPWLHLKNLTSFKPV 508
>BQ148646 weakly similar to GP|11596186|gb| cystatin-like protein {Citrus x
paradisi}, partial (34%)
Length = 645
Score = 114 bits (285), Expect = 5e-27
Identities = 66/112 (58%), Positives = 81/112 (71%)
Frame = +2
Query: 1 MRNPTLLLVLLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAK 60
MR +L+ VLL VL +Y A R Q G+SPI +INDPHV EIANFAVTE+D++SGA
Sbjct: 8 MRFQSLVPVLL---VLLAYVA-RIQAIEDGYSPIGNINDPHVIEIANFAVTEFDKKSGAT 175
Query: 61 LKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSF 112
+KFEK+I GESQ+V GTNYRLT SA S +K YEA+V+E + R LTSF
Sbjct: 176 IKFEKIIKGESQIVHGTNYRLTFSAKIDSLTKVYEAIVYEN--KSTRKLTSF 325
>TC86465 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhibitor
(Cystatin). [Cowpea] {Vigna unguiculata}, complete
Length = 660
Score = 69.3 bits (168), Expect = 2e-13
Identities = 32/75 (42%), Positives = 47/75 (62%)
Frame = +1
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N + +A FAV E++++ A L+F +VI+ + QVVAGT + +TL DG K YEA
Sbjct: 100 NSLAIDGLARFAVEEHNKKQNALLEFSRVISAKEQVVAGTIHHITLEVKDGVNKKVYEAK 279
Query: 98 VWEKIWQHFRNLTSF 112
VWEK W +F+ + F
Sbjct: 280 VWEKSWMNFKEVQEF 324
>TC77929 similar to PIR|T07139|T07139 cysteine proteinase inhibitor -
soybean, partial (80%)
Length = 965
Score = 66.2 bits (160), Expect = 2e-12
Identities = 31/75 (41%), Positives = 44/75 (58%)
Frame = +2
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N + +A FAV +++ + + L+F +V+ QVVAGT + LTL A D K YEA
Sbjct: 224 NSLEIESLARFAVDQHNAKQNSLLEFARVVKAREQVVAGTLHHLTLEAIDAGEKKIYEAK 403
Query: 98 VWEKIWQHFRNLTSF 112
VW K W +F+ LT F
Sbjct: 404 VWVKPWMNFKELTEF 448
>TC77459 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhibitor
(Cystatin). [Cowpea] {Vigna unguiculata}, partial (92%)
Length = 618
Score = 61.2 bits (147), Expect = 5e-11
Identities = 29/75 (38%), Positives = 45/75 (59%)
Frame = +3
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N + +A FAV E++ + A L+F +V+N + +VV+GT Y +TL DG K YEA
Sbjct: 114 NSVEIDNLAIFAVQEHNIKQNAVLEFVRVLNAKKKVVSGTLYDITLETKDGGKQKVYEAK 293
Query: 98 VWEKIWQHFRNLTSF 112
+ EK W +F+ + F
Sbjct: 294 ILEKPWLNFKEVQEF 338
>TC89955 similar to GP|22901842|gb|AAN10097.1 NADH dehydrogenase subunit 1
{Acryllium vulturinum}, partial (4%)
Length = 529
Score = 55.8 bits (133), Expect = 2e-09
Identities = 39/108 (36%), Positives = 64/108 (59%), Gaps = 1/108 (0%)
Frame = +1
Query: 6 LLLVLLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRR-SGAKLKFE 64
L+L++LF V + A N+ +++DP + +IA F VTE++++ + AKLKFE
Sbjct: 76 LVLIVLFASVATNQALNINRRV--------NVSDPQMIDIAKFTVTEHNKQVTEAKLKFE 231
Query: 65 KVINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSF 112
K++ G + T Y L +SA++GS S Y+++V+E HF NL F
Sbjct: 232 KLLGG---LTDETVYILAISANNGSASNIYDSLVYESPPNHF-NLKYF 363
>TC87034 homologue to SP|P13983|EXTN_TOBAC Extensin precursor (Cell wall
hydroxyproline-rich glycoprotein). [Common tobacco],
partial (2%)
Length = 644
Score = 50.1 bits (118), Expect = 1e-07
Identities = 37/120 (30%), Positives = 60/120 (49%), Gaps = 10/120 (8%)
Frame = -2
Query: 5 TLLLVLLFVVVLFSYAAARNQFAPGGWSPIDDIN-DPHVTEIANFAVTEYDRRSGAK--- 60
TL +V+ ++ + S A+ + G + I D+ + V E+ FAV EY+ + G
Sbjct: 577 TLTIVITTLLCILSTASC-GRVIVGARTEISDVGTNKEVQELGKFAVKEYNYKQGLNNGG 401
Query: 61 ----LKFEKVINGESQVVAGTNYRLTLSASD-GSYSKNYEAVVWEKIWQH-FRNLTSFVP 114
LKF +V+ E QVV+G Y L +SA D + + +VV K W H ++ + F P
Sbjct: 400 GGEGLKFVEVVEAEQQVVSGMKYYLNISAVDHNGVQRMFNSVVVVKPWLHQYKKVIHFGP 221
>BI310700 weakly similar to GP|21592712|gb| cystatin-like protein
{Arabidopsis thaliana}, partial (26%)
Length = 792
Score = 49.3 bits (116), Expect = 2e-07
Identities = 35/110 (31%), Positives = 55/110 (49%), Gaps = 9/110 (8%)
Frame = +2
Query: 5 TLLLVLLFVVVLFSYAAARNQFAPGGWSPIDDIN-DPHVTEIANFAVTEYDRRSGAK--- 60
TL +V+ ++ + S A+ + G + I D+ + V E+ FAV EY+ + G
Sbjct: 110 TLTIVITTLLCILSTASC-GRVIVGARTEISDVGTNKEVQELGKFAVKEYNYKQGLNNGG 286
Query: 61 ----LKFEKVINGESQVVAGTNYRLTLSASD-GSYSKNYEAVVWEKIWQH 105
LKF +V+ E QVV+G Y L +SA D + + +VV K W H
Sbjct: 287 GGEGLKFVEVVEAEQQVVSGMKYYLNISAVDHNGVQRMFNSVVVVKPWLH 436
>BI311369 similar to GP|11596186|gb| cystatin-like protein {Citrus x
paradisi}, partial (17%)
Length = 283
Score = 46.6 bits (109), Expect = 1e-06
Identities = 21/26 (80%), Positives = 23/26 (87%)
Frame = +1
Query: 58 GAKLKFEKVINGESQVVAGTNYRLTL 83
G LKFEKV+ GESQVVAGTNYRLT+
Sbjct: 7 GTDLKFEKVVKGESQVVAGTNYRLTI 84
>AJ502739 similar to GP|10119914|gb brain otoferlin {Mus musculus}, partial
(1%)
Length = 478
Score = 30.0 bits (66), Expect = 0.14
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +2
Query: 60 KLKFEKVINGESQVVAGTNYRLTLSASDGSY 90
KLK + V +GES+ +G NYRL+L + +Y
Sbjct: 17 KLKLKVVRSGESKDASGVNYRLSLCNNSHNY 109
>TC77500 weakly similar to SP|P21747|EA92_VICFA Embryonic abundant protein
USP92 precursor. [Broad bean] {Vicia faba}, partial
(40%)
Length = 1128
Score = 28.9 bits (63), Expect = 0.30
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = +3
Query: 60 KLKFEKVINGESQVVAGTNYRLTLSASDGS 89
++ FEKV+ QV A T Y + L ASDGS
Sbjct: 471 RMNFEKVVFYCHQVNASTTYMVPLLASDGS 560
>TC76471 similar to PIR|T51392|T51392 probable phytochelatin synthetase -
Arabidopsis thaliana, partial (94%)
Length = 1772
Score = 28.5 bits (62), Expect = 0.39
Identities = 19/60 (31%), Positives = 29/60 (47%), Gaps = 7/60 (11%)
Frame = +1
Query: 6 LLLVLLFVVVLFSYAAARNQFAPGG-----WSPIDDINDPHVTEI--ANFAVTEYDRRSG 58
LL+ + V+VLFSYAAA + P G W + D +V + +NF + + G
Sbjct: 151 LLISAICVIVLFSYAAAYDPLDPNGNITIKWDVVSWTADGYVAAVTMSNFQMYRHIMNPG 330
>TC89627 weakly similar to GP|17529290|gb|AAL38872.1 putative pectin
methylesterase {Arabidopsis thaliana}, partial (33%)
Length = 1289
Score = 27.3 bits (59), Expect = 0.88
Identities = 16/66 (24%), Positives = 28/66 (42%), Gaps = 1/66 (1%)
Frame = +1
Query: 15 VLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKLKFEKVING-ESQV 73
V+FSY N P GW +D + + + ++ + +++N E+QV
Sbjct: 904 VVFSYTYMDNSVLPKGWEDWNDTKRYMNAYYGEYKCSGPGSNTAGRVPWARMLNDKEAQV 1083
Query: 74 VAGTNY 79
GT Y
Sbjct: 1084FIGTQY 1101
>BQ144829
Length = 320
Score = 25.4 bits (54), Expect = 3.3
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +3
Query: 23 RNQFAPGGWSP 33
R FAPGGWSP
Sbjct: 102 RPSFAPGGWSP 134
>BF642440 similar to GP|4874298|gb| expressed protein {Arabidopsis thaliana},
partial (53%)
Length = 639
Score = 25.0 bits (53), Expect = 4.4
Identities = 16/67 (23%), Positives = 33/67 (48%)
Frame = -2
Query: 20 AAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNY 79
AAA FAPG W I ++++ ++ + + + ++ +K F + G ++ +
Sbjct: 434 AAATALFAPGTWYFICNLDNQYLA*VLELSAS----KASSKFSFAVLRRGMRRLSLSSQ* 267
Query: 80 RLTLSAS 86
+TLS S
Sbjct: 266 SMTLSTS 246
>TC85222 homologue to SP|P34922|G3PC_PEA Glyceraldehyde 3-phosphate
dehydrogenase cytosolic (EC 1.2.1.12). [Garden pea]
{Pisum sativum}, complete
Length = 1415
Score = 25.0 bits (53), Expect = 4.4
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = +2
Query: 3 NPTLLLVLLFVVVLFSYAAARNQFAPGGWSPIDDIN 38
N L LVL + +LF Y + + F GW D N
Sbjct: 20 NRLLFLVLSAITLLFYYFTSSSLFFSHGWCQDQDRN 127
>TC79565 similar to SP|Q9ZQ70|WRK3_ARATH Probable WRKY transcription factor 3
(WRKY DNA-binding protein 3). [Mouse-ear cress], partial
(35%)
Length = 1602
Score = 25.0 bits (53), Expect = 4.4
Identities = 8/25 (32%), Positives = 13/25 (52%)
Frame = -2
Query: 90 YSKNYEAVVWEKIWQHFRNLTSFVP 114
Y++N+ W K+W H + S P
Sbjct: 1550 YTRNWTYRPWSKLWPHIQKQKSIKP 1476
>BE325280 similar to GP|14334426|gb unknown protein {Arabidopsis thaliana},
partial (30%)
Length = 595
Score = 25.0 bits (53), Expect = 4.4
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +1
Query: 30 GWSPIDDINDPHVTEIANFAVTEYDRRSGAK 60
GW+P+ + TEIA V + R + AK
Sbjct: 124 GWNPLQEALCRRATEIATILVRHHHRSAWAK 216
>TC93880 similar to GP|19310478|gb|AAL84973.1 AT3g05350/T12H1_32
{Arabidopsis thaliana}, partial (18%)
Length = 728
Score = 25.0 bits (53), Expect = 4.4
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +1
Query: 83 LSASDGSYSKNYEAVVWEKI 102
LS ++ + NY +VVWEK+
Sbjct: 286 LSTTEIDWLNNYHSVVWEKV 345
>BI312045 similar to GP|22324956|gb| putative disease resistant protein
{Oryza sativa (japonica cultivar-group)}, partial (1%)
Length = 800
Score = 24.6 bits (52), Expect = 5.7
Identities = 13/31 (41%), Positives = 17/31 (53%)
Frame = -1
Query: 84 SASDGSYSKNYEAVVWEKIWQHFRNLTSFVP 114
S S+G SKN + V KIW R +F+P
Sbjct: 671 SESEGQKSKNQLSPVKIKIWSLVRFPIAFIP 579
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,675,352
Number of Sequences: 36976
Number of extensions: 40373
Number of successful extensions: 255
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 251
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 252
length of query: 117
length of database: 9,014,727
effective HSP length: 93
effective length of query: 24
effective length of database: 5,575,959
effective search space: 133823016
effective search space used: 133823016
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)
Medicago: description of AC148653.2