BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148528.6 + phase: 0 /partial
(310 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79458 weakly similar to PIR|D85436|D85436 MAP3K-like protein k... 313 4e-86
BQ147106 similar to GP|15451188|gb Unknown protein {Arabidopsis ... 171 3e-43
BQ149549 similar to PIR|B86270|B86 hypothetical protein AAF81295... 122 2e-28
TC93191 similar to GP|15451188|gb|AAK96865.1 Unknown protein {Ar... 80 8e-16
TC92169 similar to PIR|D85436|D85436 MAP3K-like protein kinase [... 50 8e-07
TC86001 homologue to GP|16508160|gb|AAL17948.1 phosphoinositide-... 32 0.31
BI310444 similar to GP|15451188|gb| Unknown protein {Arabidopsis... 32 0.41
TC86000 phosphoinositide-specific phospholipase C [Medicago trun... 30 0.91
TC81296 similar to GP|6707571|gb|AAF25671.1| ATP-dependent phosp... 29 2.6
TC91382 GP|21327675|gb|AAC37438.2 CPH1 {Chlamydomonas reinhardti... 28 4.5
TC83982 28 4.5
TC87413 similar to PIR|T10561|T10561 hypothetical protein F25E4.... 27 7.7
TC83463 similar to SP|Q9SZ30|HIS5_ARATH Imidazole glycerol phosp... 27 7.7
>TC79458 weakly similar to PIR|D85436|D85436 MAP3K-like protein kinase
[imported] - Arabidopsis thaliana, partial (35%)
Length = 1088
Score = 313 bits (803), Expect = 4e-86
Identities = 148/297 (49%), Positives = 208/297 (69%), Gaps = 7/297 (2%)
Frame = +1
Query: 1 QIGETCSRDVNDCGTGLQCLEC----NSQSRCTRVRTSSPISKVMELPFNHYSWLTTHNS 56
+IGETC C GL C C N++ RC+R+ +S+P++KV LPFN YSWLTTHNS
Sbjct: 205 KIGETCG----SCDAGLTCQTCPANGNTRPRCSRILSSNPVNKVKGLPFNRYSWLTTHNS 372
Query: 57 YASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDMHDYYGDIWLCR---GPCTIFT 113
+A A + S I + MNQ+D+I DQL+NGVRG MLDM+D+ D+WLC G C F+
Sbjct: 373 FAMAGARSATGSIILAPMNQDDTIADQLKNGVRGFMLDMYDFQNDVWLCHSTGGKCFNFS 552
Query: 114 AFQPAINVLREINTFLTRHRTEIVTVFIKDRVTSPNGVNKVFNKAGLRKFWFPVYKMPKN 173
+F PA+N LR++ +FL + +EI+T+FI+D V +P + KV +G+ K+ FPV ++PKN
Sbjct: 553 SFIPAVNALRDMRSFLDANPSEIITIFIEDYVRAPAALTKVIQASGINKYMFPVGRLPKN 732
Query: 174 GSDWLTVKKMLRMNHRLIVFTSNATKEASERIAYEWNYVVENKYGNDGMGRDHCLHRAES 233
GSDW TV M+ N R I F+S ++KEA+E I + W YVVEN+YG++GM C +R ES
Sbjct: 733 GSDWPTVDDMILNNQRFIAFSSRSSKEAAEGIPFTWKYVVENQYGDEGMQPGSCPNRNES 912
Query: 234 YPMNTTTKSLVLMNYYRNVLNSNEACKDNSSPLIRKMHTCYKDAGNRWPNYIAVDFY 290
PMNT ++SLVLMN++ + N ++AC DNS+PL+ + TC++ A NRWPN+IAVD+Y
Sbjct: 913 PPMNTKSRSLVLMNFFHSTPNRSQACGDNSAPLLSMLKTCHEAARNRWPNFIAVDYY 1083
>BQ147106 similar to GP|15451188|gb Unknown protein {Arabidopsis thaliana},
partial (36%)
Length = 662
Score = 171 bits (434), Expect = 3e-43
Identities = 90/185 (48%), Positives = 117/185 (62%), Gaps = 8/185 (4%)
Frame = +2
Query: 1 QIGETCSRDVNDCGTGLQCLEC-----NSQSRCTRVRTSSPISKVMELPFNHYSWLTTHN 55
++ E CS DCGTG C C ++S CTR + + S V LPFN YSW+ THN
Sbjct: 110 KVVEACSA-ATDCGTGYYCGHCPGLGRKTRSVCTRGQATLVTSIVNGLPFNKYSWIMTHN 286
Query: 56 SYASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDMHDYYGDIWLC---RGPCTIF 112
S++ A + + NQED++T+QLRNGVRG+MLDM+D+ DIWLC +G C F
Sbjct: 287 SFSIMDAPSLNGVQRLTFYNQEDTVTNQLRNGVRGLMLDMYDFQNDIWLCHSFQGQCYNF 466
Query: 113 TAFQPAINVLREINTFLTRHRTEIVTVFIKDRVTSPNGVNKVFNKAGLRKFWFPVYKMPK 172
TAFQPAIN L+E+ FLT + EIVT+ I+D V +P + +F AGL K+ FPV MPK
Sbjct: 467 TAFQPAINTLKEVEAFLTENPMEIVTIVIEDYVRTPKALINLFINAGLDKYLFPVSDMPK 646
Query: 173 NGSDW 177
NG DW
Sbjct: 647 NGEDW 661
>BQ149549 similar to PIR|B86270|B86 hypothetical protein AAF81295.1
[imported] - Arabidopsis thaliana, partial (29%)
Length = 635
Score = 122 bits (305), Expect = 2e-28
Identities = 63/146 (43%), Positives = 90/146 (61%), Gaps = 7/146 (4%)
Frame = +3
Query: 1 QIGETCSRDVNDCGTGLQCLECNSQ---SRCTRVRTSSPISKVME-LPFNHYSWLTTHNS 56
++G+ C+ D DCGTGL C +C+ + RC R + + LPFN Y++LTTHN+
Sbjct: 201 KLGDECNSD-EDCGTGLYCFDCSLEFDGKRCVRSSVTDQFKLINNSLPFNKYAFLTTHNA 377
Query: 57 YASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDMHDYYGDIWLC---RGPCTIFT 113
+A ++ NQED+IT QL NGVR +MLD +D+ D+WLC +G C FT
Sbjct: 378 FAIEGEPSHTGVPRITITNQEDTITQQLNNGVRALMLDTYDFDDDVWLCHSFQGHCHDFT 557
Query: 114 AFQPAINVLREINTFLTRHRTEIVTV 139
AF+PAI+ L+EI FL+ + EIVT+
Sbjct: 558 AFEPAIDTLKEIEAFLSANPNEIVTL 635
>TC93191 similar to GP|15451188|gb|AAK96865.1 Unknown protein {Arabidopsis
thaliana}, partial (23%)
Length = 688
Score = 80.5 bits (197), Expect = 8e-16
Identities = 38/75 (50%), Positives = 50/75 (66%)
Frame = +2
Query: 232 ESYPMNTTTKSLVLMNYYRNVLNSNEACKDNSSPLIRKMHTCYKDAGNRWPNYIAVDFYK 291
ES P+N+ T SL L NY+ E+CK+NS+PL ++TCYK AGN PN+IAV+FY
Sbjct: 50 ESKPLNSKTASLFLQNYFPTTPVEAESCKENSAPLTDMVNTCYKTAGNVLPNFIAVNFYM 229
Query: 292 RGDGGGAPEALDVAN 306
R DGGG + +D N
Sbjct: 230 RSDGGGVFDIVDRIN 274
>TC92169 similar to PIR|D85436|D85436 MAP3K-like protein kinase [imported] -
Arabidopsis thaliana, partial (6%)
Length = 505
Score = 50.4 bits (119), Expect = 8e-07
Identities = 20/30 (66%), Positives = 24/30 (79%)
Frame = +2
Query: 280 RWPNYIAVDFYKRGDGGGAPEALDVANRNL 309
RWPN+IAVD+Y R DGGG P+A+D AN L
Sbjct: 2 RWPNFIAVDYYLRSDGGGVPQAVDAANGRL 91
>TC86001 homologue to GP|16508160|gb|AAL17948.1 phosphoinositide-specific
phospholipase C {Medicago truncatula}, partial (43%)
Length = 849
Score = 32.0 bits (71), Expect = 0.31
Identities = 22/62 (35%), Positives = 31/62 (49%)
Frame = +1
Query: 34 SSPISKVMELPFNHYSWLTTHNSYASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIML 93
S+ + + M LP +HY T HNSY + LS D + I + L+ GVR I L
Sbjct: 289 STEVHQDMSLPLSHYYIFTGHNSYLT-GNQLSSDC-------SDAPIIEALQRGVRVIEL 444
Query: 94 DM 95
D+
Sbjct: 445 DI 450
>BI310444 similar to GP|15451188|gb| Unknown protein {Arabidopsis thaliana},
partial (11%)
Length = 620
Score = 31.6 bits (70), Expect = 0.41
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +3
Query: 283 NYIAVDFYKRGDGGGAPEALDVAN 306
N+IAV+FY R DGGG + +D N
Sbjct: 3 NFIAVNFYMRSDGGGVFDIVDKMN 74
>TC86000 phosphoinositide-specific phospholipase C [Medicago truncatula]
Length = 2160
Score = 30.4 bits (67), Expect = 0.91
Identities = 22/62 (35%), Positives = 30/62 (47%)
Frame = +3
Query: 34 SSPISKVMELPFNHYSWLTTHNSYASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIML 93
S+ + + M LP +HY T HNSY + LS D + I L+ GVR I L
Sbjct: 450 STGVHQDMSLPLSHYYIFTGHNSYLT-GNQLSSDC-------SDAPIIKALQRGVRVIEL 605
Query: 94 DM 95
D+
Sbjct: 606 DI 611
>TC81296 similar to GP|6707571|gb|AAF25671.1| ATP-dependent
phosphoenolpyruvate carboxykinase {Medicago sativa},
partial (44%)
Length = 813
Score = 28.9 bits (63), Expect = 2.6
Identities = 13/45 (28%), Positives = 26/45 (56%), Gaps = 5/45 (11%)
Frame = +3
Query: 241 KSLVLMNYYRNVLNSNEACKDNSSP-----LIRKMHTCYKDAGNR 280
+ L ++ ++ V++S+E C D+SSP I ++H+ K N+
Sbjct: 81 RGLPMIQTHKKVMDSSEICHDDSSPSVKAQTIHELHSLQKKKNNK 215
>TC91382 GP|21327675|gb|AAC37438.2 CPH1 {Chlamydomonas reinhardtii}, partial
(1%)
Length = 702
Score = 28.1 bits (61), Expect = 4.5
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +1
Query: 49 SWLTTHNSYASRAANL-SIDSKISSVMNQEDSITDQLRNGVRGIMLDMHDY 98
SW+ + N YAS + NL SID + S +D D L + +D+ +Y
Sbjct: 514 SWVESSNGYASASKNLISIDEQGSDHSYDDDDDDDDLSDLFMDDYMDVDNY 666
>TC83982
Length = 608
Score = 28.1 bits (61), Expect = 4.5
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = +1
Query: 13 CGTGLQCLECNSQSRCTRVRTSSPISKVMELPFNHYSWLTTHNSY 57
CG+ C CNS+ R+SS + ++PF Y W T SY
Sbjct: 238 CGSRPNCPSCNSR------RSSSGL----QMPFRPYEWHTWARSY 342
>TC87413 similar to PIR|T10561|T10561 hypothetical protein F25E4.30 -
Arabidopsis thaliana, partial (94%)
Length = 1192
Score = 27.3 bits (59), Expect = 7.7
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +2
Query: 226 HCLHRAESYPMNT 238
HC+HR+ YP+NT
Sbjct: 875 HCIHRSREYPVNT 913
>TC83463 similar to SP|Q9SZ30|HIS5_ARATH Imidazole glycerol phosphate
synthase hisHF chloroplast precursor (IGP synthase)
(ImGP synthase), partial (28%)
Length = 746
Score = 27.3 bits (59), Expect = 7.7
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 13/71 (18%)
Frame = +3
Query: 7 SRDVNDCGTG---LQCLECNSQSRCTRVRTSSPISKVMELPF---------NHYS-WLTT 53
++ V + G G L C++C+ Q + VR IS + +P NH+S T
Sbjct: 219 AKAVEELGAGEILLNCIDCDGQGKGFHVRLIKLISNAVNIPVIASSGAGIPNHFSDVFTK 398
Query: 54 HNSYASRAANL 64
N+ A+ AA +
Sbjct: 399 TNASAALAAGI 431
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.134 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,904,461
Number of Sequences: 36976
Number of extensions: 154590
Number of successful extensions: 759
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 749
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of query: 310
length of database: 9,014,727
effective HSP length: 96
effective length of query: 214
effective length of database: 5,465,031
effective search space: 1169516634
effective search space used: 1169516634
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148528.6