BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148486.12 + phase: 0 /partial
(391 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82716 weakly similar to PIR|H86331|H86331 hypothetical protein... 387 e-123
AW692234 similar to GP|10177421|dbj emb|CAB62640.1~gene_id:K1L20... 114 5e-26
BG453439 homologue to PIR|C81024|C810 hypothetical protein NMB19... 92 3e-19
TC93043 weakly similar to PIR|C84918|C84918 probable ATP-depende... 35 0.050
TC82244 similar to GP|22507458|gb|AAH19429.1 Unknown (protein fo... 35 0.065
BI271089 similar to GP|9294325|dbj| gene_id:K24M9.13~unknown pro... 33 0.25
TC83962 similar to PIR|B86289|B86289 hypothetical protein AAF719... 33 0.25
BG455206 similar to SP|Q40089|ATP4_ ATP synthase delta' chain m... 32 0.32
BF648665 weakly similar to GP|15294296|gb AT3g56820/T8M16_150 {A... 32 0.32
TC81206 similar to GP|8777433|dbj|BAA97023.1 gene_id:MHM17.4~unk... 32 0.42
AJ501021 similar to PIR|C84587|C84 hypothetical protein At2g2028... 31 0.72
TC89552 homologue to GP|21593264|gb|AAM65213.1 flower pigmentati... 30 1.6
TC88668 similar to GP|9293928|dbj|BAB01831.1 gb|AAF04880.1~gene_... 29 2.7
TC81324 similar to GP|21553871|gb|AAM62964.1 unknown {Arabidopsi... 29 2.7
TC81437 similar to GP|10177523|dbj|BAB10918. gene_id:K1F13.7~sim... 29 2.7
TC86542 weakly similar to GP|19698991|gb|AAL91231.1 EF-hand calc... 29 3.6
BG585334 similar to GP|20161490|db beta-1 3 glucanase-like prote... 29 3.6
TC80929 similar to GP|10177307|dbj|BAB10568. contains similarity... 28 4.7
TC86957 similar to GP|10178199|dbj|BAB11623. N-carbamyl-L-amino ... 28 4.7
TC81543 similar to GP|21553916|gb|AAM62999.1 unknown {Arabidopsi... 28 4.7
>TC82716 weakly similar to PIR|H86331|H86331 hypothetical protein AAG12545.1
[imported] - Arabidopsis thaliana, partial (19%)
Length = 881
Score = 387 bits (995), Expect(2) = e-123
Identities = 196/198 (98%), Positives = 197/198 (98%)
Frame = +2
Query: 1 VRLFLSEESPSQVGLNSQDHLQEKISSLLPQGEAGSDDILPPGFEGTHTSSQFDIKLSDI 60
VRLFLSEESPSQVGLNSQDHLQEKISSLLPQGEAGSDDILPPGFEGTHTSSQFDIKLSDI
Sbjct: 101 VRLFLSEESPSQVGLNSQDHLQEKISSLLPQGEAGSDDILPPGFEGTHTSSQFDIKLSDI 280
Query: 61 PVISWIKPPKIVLDLTWQVVAGEESKEIFDQHQREMRVLEAIYPRISSIPPNPISVDVDD 120
PVISWIKPPKIVLDLTWQVVAGEESKEIFDQHQREMRVLEAIYPRISSIPPNPISVDVDD
Sbjct: 281 PVISWIKPPKIVLDLTWQVVAGEESKEIFDQHQREMRVLEAIYPRISSIPPNPISVDVDD 460
Query: 121 SHYIDGHIVVIPITPVEDGDVGADTLSPGILDGSNTVTTMSMVSDVAAASVALTSLVKSS 180
SHYIDGHIVVIPITPVEDGDVGADTLSPGILDGSNTVTTMSMVSDVAAASVALTSLVKSS
Sbjct: 461 SHYIDGHIVVIPITPVEDGDVGADTLSPGILDGSNTVTTMSMVSDVAAASVALTSLVKSS 640
Query: 181 EHGNLIDPELLNDILNNP 198
EHGNLIDPELLN+ILN P
Sbjct: 641 EHGNLIDPELLNNILNIP 694
Score = 71.2 bits (173), Expect(2) = e-123
Identities = 33/39 (84%), Positives = 34/39 (86%)
Frame = +3
Query: 217 QNTGSSSNVFSHPPNPFNQVETTTSSSNAFSSTSSYTHT 255
+N NVFSHPPNPFNQVETTTSSSNAFSSTSSYTHT
Sbjct: 753 KNPDHHQNVFSHPPNPFNQVETTTSSSNAFSSTSSYTHT 869
Score = 40.0 bits (92), Expect = 0.002
Identities = 19/27 (70%), Positives = 20/27 (73%)
Frame = +1
Query: 202 EKLVRNYNVQNAQYAQNTGSSSNVFSH 228
EKLVRNYNVQNAQYAQ T +F H
Sbjct: 706 EKLVRNYNVQNAQYAQKTRIIIKMFFH 786
>AW692234 similar to GP|10177421|dbj emb|CAB62640.1~gene_id:K1L20.5~similar
to unknown protein {Arabidopsis thaliana}, partial (6%)
Length = 647
Score = 114 bits (286), Expect = 5e-26
Identities = 64/126 (50%), Positives = 82/126 (64%), Gaps = 1/126 (0%)
Frame = +2
Query: 1 VRLFLSEESPSQVGLNSQDHLQEKISSLLPQGEAGSDDILPPGFEGTHTSSQFDIKLSDI 60
V+LFLSE+ PS+VGLN QD L+ K SS+L S D LPPGFE H +Q ++ S I
Sbjct: 224 VKLFLSEDYPSKVGLNLQDSLEAKASSMLCYSTNESID-LPPGFESGHFPNQSKVQFSHI 400
Query: 61 PVISWIKPPKIVLDLTWQVVAGEESKEIFDQHQREMRVLEAIYPRISSIPPNP-ISVDVD 119
P I W P V W V AG+ES+E Q RE+RVLEA+YPR S+IPP+P +S+DV+
Sbjct: 401 PKIKWECPLSFVFSPNWLVAAGQESREKEVQKLREIRVLEAVYPRPSAIPPSPSVSLDVE 580
Query: 120 DSHYID 125
+ Y D
Sbjct: 581 EEEYDD 598
>BG453439 homologue to PIR|C81024|C810 hypothetical protein NMB1954
[imported] - Neisseria meningitidis (strain MC58
serogroup B), partial (2%)
Length = 644
Score = 92.0 bits (227), Expect = 3e-19
Identities = 65/158 (41%), Positives = 83/158 (52%), Gaps = 10/158 (6%)
Frame = +3
Query: 241 SSSNAFSSTSSYT---------HTTGGQMAPTMSTQWHRRPEISSAIAATPVEVPSTKDV 291
S+S FSS+ T HT PT ST +P +S+ +A+ V KDV
Sbjct: 45 SASFTFSSSEQSTVPLPPSGNLHTVLNNTQPTAST----KPYQTSSGSASAV-----KDV 197
Query: 292 NYYKSLIQQHGGDKQEALPYS-SSNRQNQQPLSNYDTVHNFRAKGSKPKISKPCIFFNSS 350
NYYK+LI+QHG D Q+ L S+ N Q + HN K K K CI+FNSS
Sbjct: 198 NYYKNLIRQHGADGQDKLDSQIGSHHSNFQDMK---LAHNNNQGEVKYKSKKQCIYFNSS 368
Query: 351 RGCRNGANCTFQHDASFQPQQGNPSSGMHSSKRMKMDN 388
RGCRNG++C FQHD S + GN G S+KR K+ N
Sbjct: 369 RGCRNGSDCPFQHDVSAESGAGNFLGG-QSAKRFKL*N 479
>TC93043 weakly similar to PIR|C84918|C84918 probable ATP-dependent RNA
helicase A [imported] - Arabidopsis thaliana, partial
(14%)
Length = 735
Score = 35.0 bits (79), Expect = 0.050
Identities = 13/21 (61%), Positives = 15/21 (70%)
Frame = +1
Query: 344 CIFFNSSRGCRNGANCTFQHD 364
C FF S +GCRNG +C F HD
Sbjct: 79 CKFFFSLQGCRNGGSCLFSHD 141
>TC82244 similar to GP|22507458|gb|AAH19429.1 Unknown (protein for
MGC:30371) {Mus musculus}, partial (13%)
Length = 847
Score = 34.7 bits (78), Expect = 0.065
Identities = 32/151 (21%), Positives = 61/151 (40%)
Frame = +3
Query: 213 AQYAQNTGSSSNVFSHPPNPFNQVETTTSSSNAFSSTSSYTHTTGGQMAPTMSTQWHRRP 272
A A + SS+ PP +++ TT +NAF S ++ +A
Sbjct: 384 AAAASSIRYSSDFDIAPPGVASRISTTNPWANAFPSAAAAVAAAATNVA----------- 530
Query: 273 EISSAIAATPVEVPSTKDVNYYKSLIQQHGGDKQEALPYSSSNRQNQQPLSNYDTVHNFR 332
+A +++ + D Y+ S++ G ++ + +SN ++P Y+T
Sbjct: 531 ------SAASLDLKRSSDALYHPSILSTIGQNEA----WYTSNSLAKRP--RYETGSTLP 674
Query: 333 AKGSKPKISKPCIFFNSSRGCRNGANCTFQH 363
+P K C + +R C+ G +C F H
Sbjct: 675 IYPQRPG-EKDCAHYMLTRTCKFGESCKFDH 764
>BI271089 similar to GP|9294325|dbj| gene_id:K24M9.13~unknown protein
{Arabidopsis thaliana}, partial (2%)
Length = 481
Score = 32.7 bits (73), Expect = 0.25
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = +3
Query: 340 ISKPCIFFNSSRGCRNGANCTFQHD 364
I PC FF +S CRNG +C F HD
Sbjct: 159 IDAPCKFF-ASGNCRNGKHCRFSHD 230
>TC83962 similar to PIR|B86289|B86289 hypothetical protein AAF71991.1
[imported] - Arabidopsis thaliana, partial (11%)
Length = 679
Score = 32.7 bits (73), Expect = 0.25
Identities = 31/100 (31%), Positives = 43/100 (43%), Gaps = 5/100 (5%)
Frame = +2
Query: 227 SHPPNPFNQVETTTSSSNAFSSTSSYTHTTGGQMAPT-MSTQWHRRPEISSAIAATPVE- 284
S P+P + T+TSSS++ T S + TT PT +S+ +SSA TP
Sbjct: 77 SSTPSPSSSTSTSTSSSSSPPPTPSTSSTTPSPPPPTSLSSPTPTSNHLSSA*PTTPTNT 256
Query: 285 ---VPSTKDVNYYKSLIQQHGGDKQEALPYSSSNRQNQQP 321
VPST + +L LP SS + Q P
Sbjct: 257 LTAVPSTPLNSPCSNLHLPQQTSLPSPLPSSSPSYQTTPP 376
>BG455206 similar to SP|Q40089|ATP4_ ATP synthase delta' chain mitochondrial
precursor (EC 3.6.3.14). [Sweet potato Batate], partial
(15%)
Length = 521
Score = 32.3 bits (72), Expect = 0.32
Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 3/73 (4%)
Frame = +1
Query: 4 FLSEESPSQVGLNSQDHLQEKISSLLPQG---EAGSDDILPPGFEGTHTSSQFDIKLSDI 60
FL EE+P+ G L ++ P G E + ++ PG EG F +K + +
Sbjct: 265 FLEEEAPADTGAEGDRFL---VNFYTPNGIVAEKKLEQVVLPGVEG-----YFGVKANHV 420
Query: 61 PVISWIKPPKIVL 73
P+I+ +KP + L
Sbjct: 421 PIIAQLKPGVVEL 459
>BF648665 weakly similar to GP|15294296|gb AT3g56820/T8M16_150 {Arabidopsis
thaliana}, partial (48%)
Length = 647
Score = 32.3 bits (72), Expect = 0.32
Identities = 35/143 (24%), Positives = 54/143 (37%), Gaps = 11/143 (7%)
Frame = +1
Query: 238 TTTSSSNAFSSTSSYT---------HTTGGQMAPTMSTQWHR-RPEISSAIAATPVEVPS 287
TTTSSSNA SST+S T + + + S + R RP + ++ P +P
Sbjct: 13 TTTSSSNAMSSTTSATPRRPIQISIPPSSSSSSSSTSAMFQRIRPSLVTSFLQRPHSLPF 192
Query: 288 TKDVNYYKSLIQQHGGDKQEALPYSSSNRQNQQPLSNYDT-VHNFRAKGSKPKISKPCIF 346
+ + + I LP SS N + L + + + AK ++ I P
Sbjct: 193 VLSIFLFLAWISLRSYRSSHPLPLSSPNNDVKANLRRFTSRFPSPIAKDNRGWILDPIAL 372
Query: 347 FNSSRGCRNGANCTFQHDASFQP 369
SS C H +P
Sbjct: 373 ALSSIISGGAVTCASLHLGEIRP 441
>TC81206 similar to GP|8777433|dbj|BAA97023.1 gene_id:MHM17.4~unknown
protein {Arabidopsis thaliana}, partial (5%)
Length = 842
Score = 32.0 bits (71), Expect = 0.42
Identities = 13/37 (35%), Positives = 17/37 (45%)
Frame = +3
Query: 341 SKPCIFFNSSRGCRNGANCTFQHDASFQPQQGNPSSG 377
SKPC+ + R C G +C + HD P S G
Sbjct: 195 SKPCVHYAHHRSCMKGNDCPYDHDLFNYPCSNFVSKG 305
>AJ501021 similar to PIR|C84587|C84 hypothetical protein At2g20280 [imported]
- Arabidopsis thaliana, partial (42%)
Length = 611
Score = 31.2 bits (69), Expect = 0.72
Identities = 24/87 (27%), Positives = 36/87 (40%), Gaps = 7/87 (8%)
Frame = +2
Query: 289 KDVNYYKSLIQQHGGDKQEALPYSSSNRQNQQPLSNYDTVHNFRAKGSKPKI-------S 341
K V KS +Q KQ+A + ++ L++ F+ S+PK+ S
Sbjct: 227 KYVQNLKSSVQPRTDPKQDAKKKKEEEKAKEKELNDL-----FKIAVSQPKVPPGVDPKS 391
Query: 342 KPCIFFNSSRGCRNGANCTFQHDASFQ 368
C FF + C G C F HD + Q
Sbjct: 392 VLCEFFKVGQ-CAKGFKCKFSHDLNVQ 469
>TC89552 homologue to GP|21593264|gb|AAM65213.1 flower pigmentation protein
ATAN11 {Arabidopsis thaliana}, partial (63%)
Length = 696
Score = 30.0 bits (66), Expect = 1.6
Identities = 15/43 (34%), Positives = 23/43 (52%)
Frame = +1
Query: 221 SSSNVFSHPPNPFNQVETTTSSSNAFSSTSSYTHTTGGQMAPT 263
S SN+ +HPPNP + + T T+++ S + GG PT
Sbjct: 232 SPSNILTHPPNPSSSL-TKTANAPTSSPPPPISSVFGGSPIPT 357
>TC88668 similar to GP|9293928|dbj|BAB01831.1
gb|AAF04880.1~gene_id:MFE16.2~similar to unknown protein
{Arabidopsis thaliana}, partial (39%)
Length = 802
Score = 29.3 bits (64), Expect = 2.7
Identities = 19/49 (38%), Positives = 25/49 (50%), Gaps = 3/49 (6%)
Frame = +2
Query: 222 SSNVFSHPPNPFNQVETTTSSSNAFSST---SSYTHTTGGQMAPTMSTQ 267
SSN S PP P + SSS++ SST SSY GG + +T+
Sbjct: 506 SSNKASKPPLPSLSKSASVSSSSSASSTSSSSSYCSVNGGGSSQKYTTE 652
>TC81324 similar to GP|21553871|gb|AAM62964.1 unknown {Arabidopsis
thaliana}, partial (75%)
Length = 1156
Score = 29.3 bits (64), Expect = 2.7
Identities = 15/29 (51%), Positives = 17/29 (57%)
Frame = +1
Query: 335 GSKPKISKPCIFFNSSRGCRNGANCTFQH 363
GSK SKPC F S+ GC G +C F H
Sbjct: 190 GSK---SKPCTKFFSTSGCPFGESCHFLH 267
Score = 28.1 bits (61), Expect = 6.1
Identities = 13/31 (41%), Positives = 17/31 (53%), Gaps = 2/31 (6%)
Frame = +1
Query: 335 GSKPKI--SKPCIFFNSSRGCRNGANCTFQH 363
GS P S+ C FN++ GC+ G C F H
Sbjct: 376 GSAPSAVKSRICSKFNTAEGCKFGDKCHFAH 468
>TC81437 similar to GP|10177523|dbj|BAB10918. gene_id:K1F13.7~similar to
unknown protein~sp|P55606 {Arabidopsis thaliana},
partial (47%)
Length = 1426
Score = 29.3 bits (64), Expect = 2.7
Identities = 18/65 (27%), Positives = 27/65 (40%)
Frame = -1
Query: 227 SHPPNPFNQVETTTSSSNAFSSTSSYTHTTGGQMAPTMSTQWHRRPEISSAIAATPVEVP 286
+H P TT +S A SST++ G A +RPE+ I +TP
Sbjct: 679 AHTPARTGTFFTTGKTSLAMSSTTALASANGNNPANEPLPAIRKRPELYKTIKSTPPASS 500
Query: 287 STKDV 291
+ D+
Sbjct: 499 NLADI 485
>TC86542 weakly similar to GP|19698991|gb|AAL91231.1 EF-hand calcium binding
protein-like {Arabidopsis thaliana}, partial (23%)
Length = 1085
Score = 28.9 bits (63), Expect = 3.6
Identities = 18/53 (33%), Positives = 23/53 (42%), Gaps = 7/53 (13%)
Frame = +2
Query: 207 NYNVQNAQYAQNTGSSSNVFSHPP-------NPFNQVETTTSSSNAFSSTSSY 252
NY QN Y Q S N ++ PP +P N + SSS S+S Y
Sbjct: 116 NYTSQNTNYTQPPPPSYNNYTQPPPPSSNYNHPHNYPPSQVSSSGYPPSSSGY 274
>BG585334 similar to GP|20161490|db beta-1 3 glucanase-like protein {Oryza
sativa (japonica cultivar-group)}, partial (39%)
Length = 835
Score = 28.9 bits (63), Expect = 3.6
Identities = 13/49 (26%), Positives = 26/49 (52%)
Frame = +1
Query: 218 NTGSSSNVFSHPPNPFNQVETTTSSSNAFSSTSSYTHTTGGQMAPTMST 266
+T S +N +S+PP + +S+N +S+ + T+ +PT +T
Sbjct: 265 DTTSPTNPYSNPPTSTSPYSNPPASTNPYSNPPASTNPYSNPTSPTTTT 411
>TC80929 similar to GP|10177307|dbj|BAB10568. contains similarity to zinc
finger protein~gene_id:MDC12.23 {Arabidopsis thaliana},
partial (28%)
Length = 1274
Score = 28.5 bits (62), Expect = 4.7
Identities = 21/96 (21%), Positives = 37/96 (37%), Gaps = 4/96 (4%)
Frame = +3
Query: 272 PEISSAIAATPVEVPSTKDVNYYKSLIQQHGGDKQEALPYSSSNRQNQQPL----SNYDT 327
P + I+ TP P + D N Y++ PY+ +N + +
Sbjct: 414 PFVPIIISPTPGASPRSSDWNGYQAPFYLSERSMHPPSPYAVNNPAMEMNAYMHRHKHTP 593
Query: 328 VHNFRAKGSKPKISKPCIFFNSSRGCRNGANCTFQH 363
V F + +P+ C FF + C+ ++C F H
Sbjct: 594 VEEFPERPGEPE----CSFFLKTGDCKFKSHCKFHH 689
>TC86957 similar to GP|10178199|dbj|BAB11623. N-carbamyl-L-amino acid
amidohydrolase-like protein {Arabidopsis thaliana},
partial (91%)
Length = 1590
Score = 28.5 bits (62), Expect = 4.7
Identities = 17/57 (29%), Positives = 23/57 (39%)
Frame = +2
Query: 303 GDKQEALPYSSSNRQNQQPLSNYDTVHNFRAKGSKPKISKPCIFFNSSRGCRNGANC 359
G +A+PYS L + + + G KPK S I F S R G +C
Sbjct: 425 GSHIDAIPYSGKYDGVVGVLGAVEAISVLKRSGFKPKRSLEVILFTSEEPTRFGISC 595
>TC81543 similar to GP|21553916|gb|AAM62999.1 unknown {Arabidopsis
thaliana}, partial (17%)
Length = 1364
Score = 28.5 bits (62), Expect = 4.7
Identities = 23/93 (24%), Positives = 38/93 (40%), Gaps = 4/93 (4%)
Frame = +3
Query: 178 KSSEHGNLIDPELL---NDILNNPEVIEKLVRNYNVQNAQYAQNTGSSSNVFSHPPNPFN 234
K +H I E L + +L+ P ++ + Y Q TGS + + H
Sbjct: 138 KDIDHNRHIGSESLYASSSVLSAPPMLSSIPAT--APALAYDQRTGSGTGLGMHSSRSHY 311
Query: 235 QVETTTSSSNAFSSTSSY-THTTGGQMAPTMST 266
++ + N + S SSY T+ G P +ST
Sbjct: 312 GYDSYSMEDNGYGSMSSYATNLYEGHRLPPLST 410
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.128 0.366
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,009,374
Number of Sequences: 36976
Number of extensions: 177865
Number of successful extensions: 1015
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 973
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1006
length of query: 391
length of database: 9,014,727
effective HSP length: 98
effective length of query: 293
effective length of database: 5,391,079
effective search space: 1579586147
effective search space used: 1579586147
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148486.12