BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148469.4 - phase: 0 /pseudo
(312 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83630 weakly similar to PIR|H86406|H86406 probable serine carb... 396 e-111
TC86782 similar to PIR|H86406|H86406 probable serine carboxypept... 122 4e-49
TC88970 similar to GP|16648969|gb|AAL24336.1 serine carboxypepti... 77 1e-24
CB892914 weakly similar to PIR|H86406|H86 probable serine carbox... 100 9e-22
TC77853 similar to SP|P55748|CP22_HORVU Serine carboxypeptidase ... 79 2e-15
TC85937 similar to SP|P52711|CP23_HORVU Serine carboxypeptidase ... 76 2e-14
TC89698 similar to GP|11967861|emb|CAC19488. putative serine car... 74 6e-14
BE202603 similar to GP|11967861|em putative serine carboxypeptid... 73 1e-13
TC87888 similar to PIR|G86244|G86244 Serine carboxypeptidase hom... 73 2e-13
TC77799 similar to GP|6648211|gb|AAF21209.1| putative serine car... 72 4e-13
TC92233 similar to GP|10177009|dbj|BAB10197. serine carboxypepti... 70 1e-12
TC77852 weakly similar to GP|22136958|gb|AAM91708.1 putative ser... 69 2e-12
AW689653 similar to PIR|G86244|G86 Serine carboxypeptidase homol... 68 4e-12
TC79071 similar to GP|10177810|dbj|BAB11176. serine carboxypepti... 67 9e-12
TC78092 similar to GP|7670042|dbj|BAA94996.1 serine carboxypepti... 65 3e-11
AA660359 similar to GP|22136958|gb putative serine carboxypeptid... 63 1e-10
TC88463 similar to GP|18447763|gb|AAL67992.1 putative serine car... 54 8e-08
TC88918 similar to GP|18447763|gb|AAL67992.1 putative serine car... 52 4e-07
BE999787 similar to GP|10177245|db serine carboxypeptidase {Arab... 48 4e-06
TC78484 similar to GP|6960455|gb|AAD42963.2| serine carboxypepti... 46 2e-05
>TC83630 weakly similar to PIR|H86406|H86406 probable serine
carboxypeptidase II [imported] - Arabidopsis thaliana,
partial (34%)
Length = 773
Score = 396 bits (1017), Expect = e-111
Identities = 193/193 (100%), Positives = 193/193 (100%)
Frame = +1
Query: 120 LRLVHLLILLISLKTFASHLNFSWFMRLQWKLERREMYVWKVKPPHT*IEAKCKKQFMQS 179
LRLVHLLILLISLKTFASHLNFSWFMRLQWKLERREMYVWKVKPPHT*IEAKCKKQFMQS
Sbjct: 1 LRLVHLLILLISLKTFASHLNFSWFMRLQWKLERREMYVWKVKPPHT*IEAKCKKQFMQS 180
Query: 180 W*ESPSGLHVAMSSSTTGRILRIRLYLYWGDLLGPGYELWCIVETRIRSFH*LGQSLC*R 239
W*ESPSGLHVAMSSSTTGRILRIRLYLYWGDLLGPGYELWCIVETRIRSFH*LGQSLC*R
Sbjct: 181 W*ESPSGLHVAMSSSTTGRILRIRLYLYWGDLLGPGYELWCIVETRIRSFH*LGQSLC*R 360
Query: 240 GWPRISVWTLVTTTGPGLMDHRLLDGLKLMVTSSPLQPLEERVMQPQLLNQGGPSGCFRV 299
GWPRISVWTLVTTTGPGLMDHRLLDGLKLMVTSSPLQPLEERVMQPQLLNQGGPSGCFRV
Sbjct: 361 GWPRISVWTLVTTTGPGLMDHRLLDGLKLMVTSSPLQPLEERVMQPQLLNQGGPSGCFRV 540
Query: 300 S*KQNHFQEM*PF 312
S*KQNHFQEM*PF
Sbjct: 541 S*KQNHFQEM*PF 579
>TC86782 similar to PIR|H86406|H86406 probable serine carboxypeptidase II
[imported] - Arabidopsis thaliana, partial (83%)
Length = 1531
Score = 122 bits (306), Expect(2) = 4e-49
Identities = 99/247 (40%), Positives = 124/247 (50%), Gaps = 1/247 (0%)
Frame = +1
Query: 62 WNLPLITIPGLNFYGPMD*YPWKHTGCCGLSATTPKL*VKT*MGHLVPFVIECFISLLLR 121
WN I+ L+ +G MD*Y + AT KL HL+PFV E R
Sbjct: 577 WNSTQISTLELSIFGLMD*YQIQPMIPSQKYATFHKLEDNMQTAHLLPFVQELIG*CQWR 756
Query: 122 LVHLLI-LLISLKTFASHLNFSWFMRLQWKLERREMYVWKVKPPHT*IEAKCKKQFMQSW 180
LV +LI +++ L N L K ERR M+VWK+K HT*I KCKK FM S
Sbjct: 757 LVDILIHMMLLLMCVYQQ*NNKHMCLLNCKRERR*MFVWKMKHSHT*IGKKCKKLFMLSL 936
Query: 181 *ESPSGLHVAMSSSTTGRILRIRLYLYWGDLLGPGYELWCIVETRIRSFH*LGQSLC*RG 240
* SP G V S+ T +I R + ++W LL E VE +I+ + *L L *
Sbjct: 937 *GSPHGPLVVEFSNITCKI*RFQP*IFWEHLLSQV*EFLYTVEIKIQYYR*LVHGL**MD 1116
Query: 241 WPRISVWTLVTTTGPGLMDHRLLDGLKLMVTSSPLQPLEERVMQPQLLNQGGPSGCFRVS 300
P+ SV T + GLM+ R LDG K MVT PLQP EE +M+ NQG C R
Sbjct: 1117*PKTSV*TQQSPIELGLMEDR*LDGHKYMVTCYPLQP*EEHLMRLHFHNQGDLWCC*RHF 1296
Query: 301 *KQNHFQ 307
K+NHFQ
Sbjct: 1297WKENHFQ 1317
Score = 90.1 bits (222), Expect(2) = 4e-49
Identities = 45/65 (69%), Positives = 50/65 (76%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
MLYLESPAGVGFSYS N S Y V D TARD LIFLQ W TKF +Y+N++FFITGESYA
Sbjct: 309 MLYLESPAGVGFSYSTNESFYDSVNDYLTARDNLIFLQHWFTKFSEYKNNEFFITGESYA 488
Query: 61 VWNLP 65
+P
Sbjct: 489 GHYVP 503
>TC88970 similar to GP|16648969|gb|AAL24336.1 serine carboxypeptidase II
putative {Arabidopsis thaliana}, partial (90%)
Length = 1417
Score = 77.0 bits (188), Expect(2) = 1e-24
Identities = 38/66 (57%), Positives = 47/66 (70%), Gaps = 1/66 (1%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFM-VTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESY 59
MLYLE+PAGVGFSY +S Y V D+ TARD L+FL+ W KF +Y+N D F+TGESY
Sbjct: 368 MLYLETPAGVGFSYVKGSSAYTTTVNDDETARDNLVFLERWFNKFPQYRNRDLFLTGESY 547
Query: 60 AVWNLP 65
A +P
Sbjct: 548 AGHYVP 565
Score = 53.5 bits (127), Expect(2) = 1e-24
Identities = 48/132 (36%), Positives = 63/132 (47%), Gaps = 3/132 (2%)
Frame = +3
Query: 63 NLPLITIPGLNFYGPMD*YPWKHTGCCGLSATTPKL*VKT*MGHLVPFVIECFISLLLRL 122
N+ LI+I GLN +G MD*Y + T C T +*V T FV + + +L
Sbjct: 651 NMQLISIQGLNSFGHMD*YQMQLTTCSLECVITLVM*VSTIETQFHLFVQRLWDW*VKKL 830
Query: 123 VHLLILLISLKTFASHL---NFSWFMRLQWKLERREMYVWKVKPPHT*IEAKCKKQFMQS 179
V L ++SL FA L N F+ K + MYVW + T* CK+ FMQS
Sbjct: 831 VDLWTNMMSLLMFAFPLCCHNPRLFLLNLNKQMK**MYVWMTRLQTT*TGEMCKRHFMQS 1010
Query: 180 W*ESPSGLHVAM 191
E SG+ AM
Sbjct: 1011SLEFASGMSAAM 1046
>CB892914 weakly similar to PIR|H86406|H86 probable serine carboxypeptidase
II [imported] - Arabidopsis thaliana, partial (37%)
Length = 589
Score = 100 bits (248), Expect = 9e-22
Identities = 45/60 (75%), Positives = 53/60 (88%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
MLYL+SP GVGFSYSAN+SDY++V DE TARD ++FL GW +KF KYQNS+FFITGESYA
Sbjct: 272 MLYLDSPTGVGFSYSANSSDYYLVNDEMTARDNMMFLLGWFSKFPKYQNSEFFITGESYA 451
>TC77853 similar to SP|P55748|CP22_HORVU Serine carboxypeptidase II-2
precursor (EC 3.4.16.6) (CP-MII.2) (Fragment). [Barley],
partial (78%)
Length = 1229
Score = 79.3 bits (194), Expect = 2e-15
Identities = 37/68 (54%), Positives = 50/68 (73%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+LY++SP GVGFSYS N+SD D+RTA D LIFL W +F +Y+N+DFFI+GESYA
Sbjct: 386 ILYIDSPVGVGFSYSKNSSDILTNGDKRTAEDNLIFLLKWFERFPQYKNTDFFISGESYA 565
Query: 61 VWNLPLIT 68
+P ++
Sbjct: 566 GHYVPQLS 589
>TC85937 similar to SP|P52711|CP23_HORVU Serine carboxypeptidase II-3
precursor (EC 3.4.16.6) (CP-MII.3). [Barley] {Hordeum
vulgare}, partial (44%)
Length = 1950
Score = 75.9 bits (185), Expect = 2e-14
Identities = 35/65 (53%), Positives = 47/65 (71%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L+LESPAGVGFSYS +SDY D+ TA+D +FL W+ +F +Y+ DF+ITGESYA
Sbjct: 528 VLFLESPAGVGFSYSNTSSDYDNSGDKSTAKDAYVFLINWLERFPQYKTRDFYITGESYA 707
Query: 61 VWNLP 65
+P
Sbjct: 708 GHYVP 722
>TC89698 similar to GP|11967861|emb|CAC19488. putative serine
carboxypeptidase {Pisum sativum}, partial (85%)
Length = 1333
Score = 74.3 bits (181), Expect = 6e-14
Identities = 34/65 (52%), Positives = 47/65 (72%)
Frame = +1
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L+LESP GVGFSYS +SDY D++TA D +FL W+ +F +Y+N DF+I+GESYA
Sbjct: 496 VLFLESPVGVGFSYSNKSSDYSTNGDKKTATDNYLFLVNWLERFPEYKNRDFYISGESYA 675
Query: 61 VWNLP 65
+P
Sbjct: 676 GHYVP 690
>BE202603 similar to GP|11967861|em putative serine carboxypeptidase {Pisum
sativum}, partial (26%)
Length = 403
Score = 73.2 bits (178), Expect = 1e-13
Identities = 35/63 (55%), Positives = 43/63 (67%)
Frame = +1
Query: 3 YLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYAVW 62
+LESPAGVGFSY +SDY D RTA D IFL W+ +F +Y+N DF+I GESYA
Sbjct: 1 FLESPAGVGFSYLNISSDYDTTGDRRTAADNYIFLMNWLERFPEYKNRDFYIAGESYAGH 180
Query: 63 NLP 65
+P
Sbjct: 181YVP 189
>TC87888 similar to PIR|G86244|G86244 Serine carboxypeptidase homolog
30227-33069 [imported] - Arabidopsis thaliana, partial
(57%)
Length = 1225
Score = 72.8 bits (177), Expect = 2e-13
Identities = 34/60 (56%), Positives = 40/60 (66%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
ML+LESP GVGFSYS TSDY + D+ TA D FL W KF Y++ F+I GESYA
Sbjct: 477 MLFLESPVGVGFSYSNTTSDYQQLGDDFTANDAYNFLHNWFLKFPSYRSKTFYIAGESYA 656
>TC77799 similar to GP|6648211|gb|AAF21209.1| putative serine
carboxypeptidase II {Arabidopsis thaliana}, partial
(56%)
Length = 1308
Score = 71.6 bits (174), Expect = 4e-13
Identities = 37/89 (41%), Positives = 52/89 (57%), Gaps = 11/89 (12%)
Frame = +1
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L+L+SPAGVGFSY T+D + D++TA D IFL W +F +Y++ +F+I GESYA
Sbjct: 832 ILFLDSPAGVGFSYCNKTTDLYTFGDQKTAEDAYIFLVNWFERFPQYKHREFYIAGESYA 1011
Query: 61 VWNLPL-----------ITIPGLNFYGPM 78
+P I P +NF G M
Sbjct: 1012GHYVPQLAQIVYQRNKGINNPTINFKGIM 1098
>TC92233 similar to GP|10177009|dbj|BAB10197. serine carboxypeptidase
II-like {Arabidopsis thaliana}, partial (42%)
Length = 672
Score = 70.1 bits (170), Expect = 1e-12
Identities = 33/65 (50%), Positives = 43/65 (65%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L++ESPAGVG+SYS TSDY D TA D L+F+ W KF Y++ F+TGESYA
Sbjct: 348 LLFVESPAGVGWSYSNTTSDYNNYDDTSTANDTLLFMLKWYEKFPSYRSRGLFLTGESYA 527
Query: 61 VWNLP 65
+P
Sbjct: 528 GHFIP 542
>TC77852 weakly similar to GP|22136958|gb|AAM91708.1 putative serine
carboxypeptidase II {Arabidopsis thaliana}, partial
(53%)
Length = 1016
Score = 69.3 bits (168), Expect = 2e-12
Identities = 35/70 (50%), Positives = 47/70 (67%), Gaps = 2/70 (2%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTS--DYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGES 58
+LY++SP GVGFSYS S D D+RTA D LIFL W +F +Y+ +DFFI+GES
Sbjct: 419 ILYIDSPVGVGFSYSTKNSSDDILNNGDKRTAEDNLIFLLKWFERFPQYKKTDFFISGES 598
Query: 59 YAVWNLPLIT 68
YA +P ++
Sbjct: 599 YAGHYVPQLS 628
>AW689653 similar to PIR|G86244|G86 Serine carboxypeptidase homolog
30227-33069 [imported] - Arabidopsis thaliana, partial
(40%)
Length = 697
Score = 68.2 bits (165), Expect = 4e-12
Identities = 31/60 (51%), Positives = 38/60 (62%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
ML+LESP GVGFSYS TS+Y + D+ A D FL W K+ Y+ F+I GESYA
Sbjct: 345 MLFLESPVGVGFSYSNTTSEYAQLGDDFAANDAYTFLHNWFLKYPSYRTRAFYIAGESYA 524
>TC79071 similar to GP|10177810|dbj|BAB11176. serine carboxypeptidase
II-like protein {Arabidopsis thaliana}, partial (40%)
Length = 1123
Score = 67.0 bits (162), Expect = 9e-12
Identities = 32/68 (47%), Positives = 46/68 (67%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L+LESPAGVGFSY+ TSD + D TA+D FL W +F ++++ DF+I GESYA
Sbjct: 758 LLFLESPAGVGFSYTNTTSDISELGDTITAKDSHTFLINWFKRFPQFKSHDFYIAGESYA 937
Query: 61 VWNLPLIT 68
+P ++
Sbjct: 938 GHYVPQLS 961
>TC78092 similar to GP|7670042|dbj|BAA94996.1 serine carboxypeptidase
II-like protein {Arabidopsis thaliana}, partial (71%)
Length = 1724
Score = 65.1 bits (157), Expect = 3e-11
Identities = 29/65 (44%), Positives = 43/65 (65%)
Frame = +1
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L++ESP GVGFSY+ +SD ++ D A D FL W+ +F ++++ DFFI GESYA
Sbjct: 421 LLFVESPVGVGFSYTNTSSDLTILEDHFVAEDAYNFLVNWLQRFPQFKSRDFFIAGESYA 600
Query: 61 VWNLP 65
+P
Sbjct: 601 GHYIP 615
>AA660359 similar to GP|22136958|gb putative serine carboxypeptidase II
{Arabidopsis thaliana}, partial (30%)
Length = 594
Score = 63.2 bits (152), Expect = 1e-10
Identities = 30/68 (44%), Positives = 42/68 (61%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L ++SP G GFSYS +SD D+RT D LIFL W +F +Y+ +DFFI G YA
Sbjct: 345 ILLIDSPVGXGFSYSNASSDILNNGDKRTTEDSLIFLLKWFERFPRYKETDFFINGXXYA 524
Query: 61 VWNLPLIT 68
+P ++
Sbjct: 525 GHYVPQLS 548
>TC88463 similar to GP|18447763|gb|AAL67992.1 putative serine
carboxypeptidase precursor {Gossypium hirsutum}, partial
(71%)
Length = 1452
Score = 53.9 bits (128), Expect = 8e-08
Identities = 26/67 (38%), Positives = 40/67 (58%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+LY++ P G GFSYS + D ++ + D+ FLQ + + +Y +DFFITGESYA
Sbjct: 540 LLYVDQPTGTGFSYSTDLRD-IRHNEKGVSNDLYDFLQAFFAEHPQYAKNDFFITGESYA 716
Query: 61 VWNLPLI 67
+P +
Sbjct: 717 GHYIPAL 737
>TC88918 similar to GP|18447763|gb|AAL67992.1 putative serine
carboxypeptidase precursor {Gossypium hirsutum}, partial
(58%)
Length = 1145
Score = 51.6 bits (122), Expect = 4e-07
Identities = 25/65 (38%), Positives = 39/65 (59%)
Frame = +1
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+LY++ P G GFSY+ + D ++ + D+ FLQ + + +Y +DFFITGESYA
Sbjct: 493 LLYVDQPTGTGFSYTTDKRD-IRHDEDGVSNDLYDFLQAFFGEHPEYATNDFFITGESYA 669
Query: 61 VWNLP 65
+P
Sbjct: 670 GHYIP 684
>BE999787 similar to GP|10177245|db serine carboxypeptidase {Arabidopsis
thaliana}, partial (32%)
Length = 672
Score = 48.1 bits (113), Expect = 4e-06
Identities = 26/66 (39%), Positives = 41/66 (61%), Gaps = 1/66 (1%)
Frame = +1
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDER-TARDVLIFLQGWVTKFQKYQNSDFFITGESY 59
+L+++ P G GFSY++N D + DE + D+ FLQ + + K+ +DF+ITGESY
Sbjct: 481 ILFVDQPIGTGFSYTSNEDD--IPHDETGVSNDLYDFLQEFFKQHPKFIKNDFYITGESY 654
Query: 60 AVWNLP 65
A +P
Sbjct: 655 AGHYVP 672
>TC78484 similar to GP|6960455|gb|AAD42963.2| serine carboxypeptidase
precursor {Matricaria chamomilla}, partial (34%)
Length = 788
Score = 45.8 bits (107), Expect = 2e-05
Identities = 21/65 (32%), Positives = 39/65 (59%)
Frame = +1
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+++++ P G GFSY+ + SD ++ + D+ FLQ + + ++ +DF+ITG SYA
Sbjct: 532 IIFVDQPTGTGFSYTTDDSDV-RHDEDGVSNDLYDFLQAFFKEHPQFTKNDFYITGXSYA 708
Query: 61 VWNLP 65
+P
Sbjct: 709 GHYIP 723
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.340 0.150 0.525
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,046,102
Number of Sequences: 36976
Number of extensions: 197143
Number of successful extensions: 1647
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 1620
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1636
length of query: 312
length of database: 9,014,727
effective HSP length: 96
effective length of query: 216
effective length of database: 5,465,031
effective search space: 1180446696
effective search space used: 1180446696
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148469.4