BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148406.5 - phase: 0 /pseudo
(363 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87655 similar to GP|4587225|dbj|BAA36171.1 FEN-1 {Oryza sativa... 681 0.0
TC84018 similar to PIR|F86150|F86150 hypothetical protein AAF764... 62 3e-10
TC92628 similar to PIR|E86419|E86419 probable exonuclease 92014... 33 0.23
TC80589 similar to PIR|B86468|B86468 probable DNA polymerase typ... 30 1.9
TC83286 similar to PIR|B84919|B84919 Not56-like protein [importe... 29 3.3
BF632546 similar to GP|18033111|gb functional candidate resistan... 27 9.5
TC88246 similar to PIR|T51328|T51328 transcription initiation fa... 27 9.5
BG580721 GP|4185511|gb| actin depolymerizing factor 4 {Arabidops... 27 9.5
TC93151 homologue to GP|22136928|gb|AAM91808.1 unknown protein {... 27 9.5
>TC87655 similar to GP|4587225|dbj|BAA36171.1 FEN-1 {Oryza sativa (japonica
cultivar-group)}, partial (92%)
Length = 1120
Score = 681 bits (1756), Expect = 0.0
Identities = 342/343 (99%), Positives = 343/343 (99%)
Frame = +2
Query: 1 MKENKFESYFGRKIAVDASMSIYQFLIVVGRSGTEMLTNEAGEVTSHLQGMFARTIRLLE 60
MKENKFESYFGRKIAVDASMSIYQFLIVVGRSGTEMLTNEAGEVTSHLQGMFARTIRLLE
Sbjct: 92 MKENKFESYFGRKIAVDASMSIYQFLIVVGRSGTEMLTNEAGEVTSHLQGMFARTIRLLE 271
Query: 61 AGMKPVYVFDGKPPEMKNQELKKRLSKRAEATAGLTEALEADNKEDIEKFSKRTVKVTKQ 120
AGMKPVYVFDGKPPEMKNQELKKRLSKRAEATAGLTEALEADNKEDIEKFSKRTVKVTKQ
Sbjct: 272 AGMKPVYVFDGKPPEMKNQELKKRLSKRAEATAGLTEALEADNKEDIEKFSKRTVKVTKQ 451
Query: 121 HNDDCKRLLRLMGVPVVEAPSEAEAQCAALCKAGKVYAVASEDMDSLTFGAPKFLRHLMD 180
HNDDCKRLLRLMGVPVVEAPSEAEAQCAALCKAGKVYAVASEDMDSLTFGAPKFLRHLMD
Sbjct: 452 HNDDCKRLLRLMGVPVVEAPSEAEAQCAALCKAGKVYAVASEDMDSLTFGAPKFLRHLMD 631
Query: 181 PSSKKIPVMEFDVAKILEELDLTMDQFIDLCILSGCDYCDNIRGIGGMTALKLIRQHGSI 240
PSSKKIPVMEFDVAKILEELDLTMDQFIDLCILSGCDYCDNIRGIGGMTALKLIRQHGSI
Sbjct: 632 PSSKKIPVMEFDVAKILEELDLTMDQFIDLCILSGCDYCDNIRGIGGMTALKLIRQHGSI 811
Query: 241 EKILENISKERYQVPDDWPYQEARRLFKEPEVSTDDEVLNLKWSPPDEEGLITFLVNENG 300
EKILENISKERYQVPDDWPYQEARRLFKEPEVSTDDEVLNLKWSPPDEEGLITFLVNENG
Sbjct: 812 EKILENISKERYQVPDDWPYQEARRLFKEPEVSTDDEVLNLKWSPPDEEGLITFLVNENG 991
Query: 301 FNSDRVTKAIEKIKAAKNKSSQGRLESFFKPTANPSVPIKRKQ 343
FNSDRVTKAIEKIKAAKNKSSQGRLESFFKPTANPSVPIKRK+
Sbjct: 992 FNSDRVTKAIEKIKAAKNKSSQGRLESFFKPTANPSVPIKRKE 1120
>TC84018 similar to PIR|F86150|F86150 hypothetical protein AAF76467.1
[imported] - Arabidopsis thaliana, partial (38%)
Length = 711
Score = 62.4 bits (150), Expect = 3e-10
Identities = 47/140 (33%), Positives = 68/140 (48%), Gaps = 1/140 (0%)
Frame = +2
Query: 47 HLQGMFARTIRLLEA-GMKPVYVFDGKPPEMKNQELKKRLSKRAEATAGLTEALEADNKE 105
HL+ F RTI L G PV+V DG P +K+Q R + ++G+ +E
Sbjct: 293 HLRLTFFRTINLFSKFGAFPVFVVDGTPSPLKSQARIARFFR----SSGIESTSLPVAEE 460
Query: 106 DIEKFSKRTVKVTKQHNDDCKRLLRLMGVPVVEAPSEAEAQCAALCKAGKVYAVASEDMD 165
+ T Q +C L +L+G+PV++A EAEA CA L G V A + D D
Sbjct: 461 GVSPGRNSTFSRCVQ---ECVELAKLLGIPVLKAKGEAEALCAQLNSEGHVDACITPDSD 631
Query: 166 SLTFGAPKFLRHLMDPSSKK 185
+ FGA K + P+SK+
Sbjct: 632 AFLFGA-KCIIKSFSPNSKE 688
>TC92628 similar to PIR|E86419|E86419 probable exonuclease 92014-93872
[imported] - Arabidopsis thaliana, partial (57%)
Length = 676
Score = 32.7 bits (73), Expect = 0.23
Identities = 25/85 (29%), Positives = 34/85 (39%)
Frame = +1
Query: 45 TSHLQGMFARTIRLLEAGMKPVYVFDGKPPEMKNQELKKRLSKRAEATAGLTEALEADNK 104
T H++ R L G+KP+ VFDG MK + KR R E L A+E +
Sbjct: 217 TRHIEYCMHRVNLLRHYGVKPILVFDGGLLPMKGDQENKRARSRKE---NLERAVEHEAN 387
Query: 105 EDIEKFSKRTVKVTKQHNDDCKRLL 129
+ + K DC R L
Sbjct: 388 GNSTAAYECYQKAV*YFTSDCTRTL 462
>TC80589 similar to PIR|B86468|B86468 probable DNA polymerase type I
54894-56354 [imported] - Arabidopsis thaliana, partial
(67%)
Length = 1416
Score = 29.6 bits (65), Expect = 1.9
Identities = 18/41 (43%), Positives = 24/41 (57%), Gaps = 7/41 (17%)
Frame = +1
Query: 211 CILSGCDYCDNIRGI-------GGMTALKLIRQHGSIEKIL 244
CI+ D D + GI G TALKLI++HGS+E +L
Sbjct: 523 CIIG--DEVDGVPGIQHLVPSFGWKTALKLIQKHGSLEALL 639
>TC83286 similar to PIR|B84919|B84919 Not56-like protein [imported] -
Arabidopsis thaliana, partial (45%)
Length = 845
Score = 28.9 bits (63), Expect = 3.3
Identities = 17/47 (36%), Positives = 23/47 (48%)
Frame = -3
Query: 150 LCKAGKVYAVASEDMDSLTFGAPKFLRHLMDPSSKKIPVMEFDVAKI 196
+CK K Y V+S+ D +FG + R D P M+ D AKI
Sbjct: 375 MCKCSKCYEVSSQQKDCKSFGYKYWFR---DEFEVNRPEMDEDSAKI 244
>BF632546 similar to GP|18033111|gb functional candidate resistance protein
KR1 {Glycine max}, partial (8%)
Length = 672
Score = 27.3 bits (59), Expect = 9.5
Identities = 12/56 (21%), Positives = 33/56 (58%), Gaps = 9/56 (16%)
Frame = +2
Query: 239 SIEKILENISKE--RYQVPDD-------WPYQEARRLFKEPEVSTDDEVLNLKWSP 285
++ ++E++ KE R + P+D W Y++ +++FKE ++ ++++ ++ P
Sbjct: 167 TLHDLIEDMGKEIVRRESPEDPGKRTRLWAYEDIKKVFKENTGTSTIKIIHFQFDP 334
>TC88246 similar to PIR|T51328|T51328 transcription initiation factor sigma5
plastid -specific [imported] - Arabidopsis thaliana,
partial (42%)
Length = 1158
Score = 27.3 bits (59), Expect = 9.5
Identities = 25/94 (26%), Positives = 48/94 (50%), Gaps = 6/94 (6%)
Frame = +3
Query: 232 KLIRQHGSIEKILENISKERYQVPDDWPYQEARRLFK--EPEVST----DDEVLNLKWSP 285
+L+R++ S+ L ++ ++ ++P P E LFK +P + DD L+ P
Sbjct: 678 ELLREY-SVPADLVSLDWKKMKIPPVLPSSEHAFLFKLMQPMKALLQVKDDLQKELEREP 854
Query: 286 PDEEGLITFLVNENGFNSDRVTKAIEKIKAAKNK 319
++E + + ++ +V KAIE +AA+NK
Sbjct: 855 TEDE-----IADATNMHTTQVKKAIEVGRAARNK 941
>BG580721 GP|4185511|gb| actin depolymerizing factor 4 {Arabidopsis
thaliana}, partial (9%)
Length = 563
Score = 27.3 bits (59), Expect = 9.5
Identities = 17/56 (30%), Positives = 25/56 (44%)
Frame = -2
Query: 299 NGFNSDRVTKAIEKIKAAKNKSSQGRLESFFKPTANPSVPIKRKQLIIRCPLCEFL 354
+GF R K K NKS + + ++ NP IK Q+I CPL + +
Sbjct: 454 DGFFDRRERKRTRNYKIIINKSQENKNYKYWALYQNP---IKVNQMISECPLLKII 296
>TC93151 homologue to GP|22136928|gb|AAM91808.1 unknown protein {Arabidopsis
thaliana}, partial (4%)
Length = 657
Score = 27.3 bits (59), Expect = 9.5
Identities = 14/37 (37%), Positives = 21/37 (55%)
Frame = -3
Query: 70 DGKPPEMKNQELKKRLSKRAEATAGLTEALEADNKED 106
+G PP + + + A+A AG+ EA EA NKE+
Sbjct: 391 EGVPPPNGEPAVAEPKMEGADAAAGVEEAGEAPNKEE 281
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.135 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,497,080
Number of Sequences: 36976
Number of extensions: 108288
Number of successful extensions: 531
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 530
length of query: 363
length of database: 9,014,727
effective HSP length: 97
effective length of query: 266
effective length of database: 5,428,055
effective search space: 1443862630
effective search space used: 1443862630
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148406.5