Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC148406.1 + phase: 0 
         (114 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BQ154200 similar to GP|14488090|gb|A AT5g26741 {Arabidopsis thal...   215  2e-59
TC80370 similar to GP|21554331|gb|AAM63438.1 unknown {Arabidopsi...    32  0.036
TC80584 similar to PIR|T45688|T45688 hypothetical protein F18L15...    29  0.24
BQ151385 similar to GP|21537149|gb 60S ribosomal protein L13  BB...    27  0.90
TC77567 similar to PIR|S56719|S56719 serine/threonine-specific p...    27  1.5
TC77906 similar to GP|21553945|gb|AAM63026.1 unknown {Arabidopsi...    27  1.5
TC91481 homologue to GP|22135922|gb|AAM91543.1 phosphoserine ami...    26  2.0
TC77894 similar to GP|12583793|gb|AAG59645.1 hypothetical protei...    26  2.6
TC90626 similar to GP|15289892|dbj|BAB63587. hypothetical protei...    25  3.4
TC82045 similar to GP|13898655|gb|AAK48848.1 expansin {Prunus ce...    25  3.4
AW776463 homologue to SP|O23787|THI4 Thiazole biosynthetic enzym...    25  3.4
TC80103 weakly similar to PIR|H96561|H96561 probable peptide tra...    25  3.4
TC88644 similar to GP|21553998|gb|AAM63079.1 unknown {Arabidopsi...    25  3.4
TC81437 similar to GP|10177523|dbj|BAB10918. gene_id:K1F13.7~sim...    25  3.4
AL375832                                                               25  4.4
TC83294 weakly similar to PIR|S46797|S46797 ribosomal protein L1...    25  4.4
TC78954 weakly similar to PIR|D96810|D96810 hypothetical protein...    25  4.4
TC78517 similar to SP|Q9SSK1|SYN3_ARATH Asparaginyl-tRNA synthet...    25  5.8
AJ388803                                                               25  5.8
TC90228 similar to PIR|T47675|T47675 glycoprotein-like - Arabido...    25  5.8

>BQ154200 similar to GP|14488090|gb|A AT5g26741 {Arabidopsis thaliana},
           partial (29%)
          Length = 464

 Score =  215 bits (547), Expect(2) = 2e-59
 Identities = 102/102 (100%), Positives = 102/102 (100%)
 Frame = +1

Query: 13  ESFGYYNFPDQNYTMIEKRQLFLRSYQFCRKKSLTERIKGSLVRAKKVVWLKLRYARGLR 72
           ESFGYYNFPDQNYTMIEKRQLFLRSYQFCRKKSLTERIKGSLVRAKKVVWLKLRYARGLR
Sbjct: 91  ESFGYYNFPDQNYTMIEKRQLFLRSYQFCRKKSLTERIKGSLVRAKKVVWLKLRYARGLR 270

Query: 73  RKLVFFPRFKCGFYYRRRRFSQLLNNSGSSHHRKVDSSSCLW 114
           RKLVFFPRFKCGFYYRRRRFSQLLNNSGSSHHRKVDSSSCLW
Sbjct: 271 RKLVFFPRFKCGFYYRRRRFSQLLNNSGSSHHRKVDSSSCLW 396



 Score = 28.5 bits (62), Expect(2) = 2e-59
 Identities = 12/12 (100%), Positives = 12/12 (100%)
 Frame = +3

Query: 1  MGSVTMNPLFRT 12
          MGSVTMNPLFRT
Sbjct: 54 MGSVTMNPLFRT 89


>TC80370 similar to GP|21554331|gb|AAM63438.1 unknown {Arabidopsis
           thaliana}, partial (32%)
          Length = 808

 Score = 32.0 bits (71), Expect = 0.036
 Identities = 26/72 (36%), Positives = 37/72 (51%), Gaps = 6/72 (8%)
 Frame = +1

Query: 11  RTESFGYYNFPDQNYTMIEK---RQLFLRSYQFCR--KKSLTERIKGSLVRAK-KVVWLK 64
           R+ S G  N   Q+  +++    RQL+LRSY FCR  +K + E+ K    R K K V  K
Sbjct: 409 RSVSSGRENGLQQHPRVVDSISCRQLYLRSYTFCRDDEKKVLEKTKKCFGRVKVKKVKAK 588

Query: 65  LRYARGLRRKLV 76
            R   G + K +
Sbjct: 589 ERVVIGKKAKKI 624


>TC80584 similar to PIR|T45688|T45688 hypothetical protein F18L15.30 -
           Arabidopsis thaliana, partial (15%)
          Length = 714

 Score = 29.3 bits (64), Expect = 0.24
 Identities = 25/91 (27%), Positives = 42/91 (45%), Gaps = 8/91 (8%)
 Frame = +2

Query: 31  RQLFLRSYQFCRKK-SLTER-------IKGSLVRAKKVVWLKLRYARGLRRKLVFFPRFK 82
           RQ++LRSY+F RKK S+TE+       +K S++ A  ++    +  R +  +        
Sbjct: 206 RQVYLRSYKFSRKKLSVTEKTIKYFSSLKESVICASNILCCNSKGNRKIPGRAKHITHAA 385

Query: 83  CGFYYRRRRFSQLLNNSGSSHHRKVDSSSCL 113
              Y+R      LL+ S   H  + D   C+
Sbjct: 386 VSVYHR------LLSRSDKVHVARHDF*CCV 460


>BQ151385 similar to GP|21537149|gb 60S ribosomal protein L13  BBC1 protein
           {Arabidopsis thaliana}, partial (55%)
          Length = 720

 Score = 27.3 bits (59), Expect = 0.90
 Identities = 18/43 (41%), Positives = 22/43 (50%)
 Frame = +2

Query: 53  SLVRAKKVVWLKLRYARGLRRKLVFFPRFKCGFYYRRRRFSQL 95
           S VRA+KV+WL +   R   R  +   RFK   Y   RR S L
Sbjct: 410 SHVRARKVIWLVIPLPRNYARCYI---RFKVHTYQLVRRESHL 529


>TC77567 similar to PIR|S56719|S56719 serine/threonine-specific protein
           kinase SPK-1 (EC 2.7.1.-) - soybean, partial (64%)
          Length = 744

 Score = 26.6 bits (57), Expect = 1.5
 Identities = 15/35 (42%), Positives = 23/35 (64%), Gaps = 4/35 (11%)
 Frame = +1

Query: 21  PDQNY-TMIEKRQLFLRS---YQFCRKKSLTERIK 51
           P Q Y T+ +++QL LR     +FC K+S TER++
Sbjct: 487 PSQQYCTLNQRQQLDLRHTLLLRFCYKRSTTERLQ 591


>TC77906 similar to GP|21553945|gb|AAM63026.1 unknown {Arabidopsis
           thaliana}, partial (92%)
          Length = 1371

 Score = 26.6 bits (57), Expect = 1.5
 Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 2/44 (4%)
 Frame = +2

Query: 73  RKLVFFPRFKC--GFYYRRRRFSQLLNNSGSSHHRKVDSSSCLW 114
           R+ ++F +  C  GF++R   F  LL + GS  H  +  S  LW
Sbjct: 125 RRPIWFHKCSCYKGFHHRFSAFHNLLWDPGSFQHFGLVLSGYLW 256


>TC91481 homologue to GP|22135922|gb|AAM91543.1 phosphoserine
          aminotransferase {Arabidopsis thaliana}, partial (25%)
          Length = 607

 Score = 26.2 bits (56), Expect = 2.0
 Identities = 14/19 (73%), Positives = 15/19 (78%)
 Frame = +2

Query: 59 KVVWLKLRYARGLRRKLVF 77
          KVVWLKLR  R +RRKL F
Sbjct: 17 KVVWLKLR--RRIRRKLRF 67


>TC77894 similar to GP|12583793|gb|AAG59645.1 hypothetical protein {Oryza
           sativa}, partial (84%)
          Length = 837

 Score = 25.8 bits (55), Expect = 2.6
 Identities = 12/28 (42%), Positives = 17/28 (59%), Gaps = 1/28 (3%)
 Frame = +1

Query: 88  RRRRFSQL-LNNSGSSHHRKVDSSSCLW 114
           R+ RFS+    N  +SH R++  S CLW
Sbjct: 202 RKYRFSRRGCRNRVASHPRRLSQSQCLW 285


>TC90626 similar to GP|15289892|dbj|BAB63587. hypothetical protein {Oryza
           sativa (japonica cultivar-group)}, partial (12%)
          Length = 519

 Score = 25.4 bits (54), Expect = 3.4
 Identities = 13/39 (33%), Positives = 24/39 (61%)
 Frame = +2

Query: 73  RKLVFFPRFKCGFYYRRRRFSQLLNNSGSSHHRKVDSSS 111
           R ++FF +F C F++     S++LN   +SH+  V ++S
Sbjct: 11  RTIIFFFQFSCSFHHSYES-SKILNYFIASHNSVVCNAS 124


>TC82045 similar to GP|13898655|gb|AAK48848.1 expansin {Prunus cerasus},
           partial (91%)
          Length = 814

 Score = 25.4 bits (54), Expect = 3.4
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = -2

Query: 41  CRKKSLTERIKGSLVRAKKVVWLKLRYARGLRRK 74
           CR++   ER         + VWL+L+   GLRR+
Sbjct: 141 CRRRRWRERFAMLRRSRHRGVWLRLK*GEGLRRR 40


>AW776463 homologue to SP|O23787|THI4 Thiazole biosynthetic enzyme
           chloroplast precursor. [Sweet orange] {Citrus sinensis},
           partial (44%)
          Length = 644

 Score = 25.4 bits (54), Expect = 3.4
 Identities = 22/57 (38%), Positives = 26/57 (45%)
 Frame = -3

Query: 50  IKGSLVRAKKVVWLKLRYARGLRRKLVFFPRFKCGFYYRRRRFSQLLNNSGSSHHRK 106
           IK S   A K  W+ +R  R  RR      R +C     RRR S L  N GS H R+
Sbjct: 372 IKSSAATALKSFWI-IRKRRKDRRNRHRL*RRRC-----RRR*SCLSCNDGSFHERR 220


>TC80103 weakly similar to PIR|H96561|H96561 probable peptide transporter
            [imported] - Arabidopsis thaliana, partial (23%)
          Length = 1236

 Score = 25.4 bits (54), Expect = 3.4
 Identities = 10/31 (32%), Positives = 19/31 (61%)
 Frame = +1

Query: 9    LFRTESFGYYNFPDQNYTMIEKRQLFLRSYQ 39
            L RTE+F Y+     N+T+ ++ Q+ +  +Q
Sbjct: 1009 LIRTENFNYHLRTHLNFTITKRTQILILLFQ 1101


>TC88644 similar to GP|21553998|gb|AAM63079.1 unknown {Arabidopsis
           thaliana}, partial (68%)
          Length = 896

 Score = 25.4 bits (54), Expect = 3.4
 Identities = 10/13 (76%), Positives = 12/13 (91%)
 Frame = +1

Query: 101 SSHHRKVDSSSCL 113
           S H+R+VDSSSCL
Sbjct: 418 SPHNRRVDSSSCL 456


>TC81437 similar to GP|10177523|dbj|BAB10918. gene_id:K1F13.7~similar to
           unknown protein~sp|P55606 {Arabidopsis thaliana},
           partial (47%)
          Length = 1426

 Score = 25.4 bits (54), Expect = 3.4
 Identities = 13/38 (34%), Positives = 19/38 (49%)
 Frame = +3

Query: 2   GSVTMNPLFRTESFGYYNFPDQNYTMIEKRQLFLRSYQ 39
           GSV+    F T  +    FPD     +EK QL L+ ++
Sbjct: 321 GSVSEKSSFGTIVYAPSEFPDAKPETLEKTQLILQQFK 434


>AL375832 
          Length = 535

 Score = 25.0 bits (53), Expect = 4.4
 Identities = 14/38 (36%), Positives = 22/38 (57%)
 Frame = -1

Query: 29  EKRQLFLRSYQFCRKKSLTERIKGSLVRAKKVVWLKLR 66
           +KRQ      Q   K +LTE I  +L RA+ +++LK +
Sbjct: 262 KKRQFLFIKRQCRNKHALTENITLALGRARGLLFLKTK 149


>TC83294 weakly similar to PIR|S46797|S46797 ribosomal protein L14.e.B
           cytosolic - yeast (Saccharomyces cerevisiae), partial
           (34%)
          Length = 477

 Score = 25.0 bits (53), Expect = 4.4
 Identities = 15/39 (38%), Positives = 18/39 (45%)
 Frame = +2

Query: 54  LVRAKKVVWLKLRYARGLRRKLVFFPRFKCGFYYRRRRF 92
           L + KK  W K    R  R  L  F RFK     ++RRF
Sbjct: 290 LAKWKKSSWAKKLEDRKKRSVLTDFDRFKLSKLKKQRRF 406


>TC78954 weakly similar to PIR|D96810|D96810 hypothetical protein T11I11.6
           [imported] - Arabidopsis thaliana, partial (45%)
          Length = 2329

 Score = 25.0 bits (53), Expect = 4.4
 Identities = 9/21 (42%), Positives = 14/21 (65%)
 Frame = +2

Query: 29  EKRQLFLRSYQFCRKKSLTER 49
           +K   F  +Y+FC+K + TER
Sbjct: 443 KKHHFFSHTYRFCKKLNSTER 505


>TC78517 similar to SP|Q9SSK1|SYN3_ARATH Asparaginyl-tRNA synthetase
           cytoplasmic 3 (EC 6.1.1.22) (Asparagine-- tRNA ligase 3)
           (AsnRS 3)., partial (37%)
          Length = 795

 Score = 24.6 bits (52), Expect = 5.8
 Identities = 14/38 (36%), Positives = 19/38 (49%)
 Frame = -3

Query: 74  KLVFFPRFKCGFYYRRRRFSQLLNNSGSSHHRKVDSSS 111
           K +F  +    F  RRR+F   LNN+ SS   K+   S
Sbjct: 415 KNLFSTKLDLLFRSRRRKFEMSLNNNTSSSLNKLSKIS 302


>AJ388803 
          Length = 442

 Score = 24.6 bits (52), Expect = 5.8
 Identities = 11/35 (31%), Positives = 19/35 (53%)
 Frame = +1

Query: 79  PRFKCGFYYRRRRFSQLLNNSGSSHHRKVDSSSCL 113
           P  K     +++  +Q  N+S    HR+ DS++CL
Sbjct: 133 PSHKINRRIQKKSSNQTSNSSDPPFHRQEDSNTCL 237


>TC90228 similar to PIR|T47675|T47675 glycoprotein-like - Arabidopsis
          thaliana, complete
          Length = 1312

 Score = 24.6 bits (52), Expect = 5.8
 Identities = 8/17 (47%), Positives = 12/17 (70%)
 Frame = +3

Query: 71 LRRKLVFFPRFKCGFYY 87
          L R +  FP+F+C FY+
Sbjct: 15 LXRNITLFPQFRC*FYW 65


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.329    0.140    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,285,345
Number of Sequences: 36976
Number of extensions: 58649
Number of successful extensions: 408
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 407
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 408
length of query: 114
length of database: 9,014,727
effective HSP length: 90
effective length of query: 24
effective length of database: 5,686,887
effective search space: 136485288
effective search space used: 136485288
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 50 (23.9 bits)


Medicago: description of AC148406.1