Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC148404.10 + phase: 0 
         (400 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC79535 weakly similar to GP|10178054|dbj|BAB11418. gene_id:MUB3...    86  3e-17
BG648384 similar to GP|14718995|gb ceramide glucosyltransferase ...    29  2.8
TC80121 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precurs...    29  2.8
AW585898 weakly similar to GP|15983422|gb| At2g19880/F6F22.9 {Ar...    29  2.8
TC86336 similar to GP|14626761|gb|AAK71662.1 mature anther-speci...    29  3.7
TC86335 similar to GP|14626761|gb|AAK71662.1 mature anther-speci...    29  3.7
TC77680 similar to GP|18650600|gb|AAL75900.1 AT4g38220/F20D10_34...    28  4.8
TC78429 similar to GP|16604607|gb|AAL24096.1 putative AtBgamma p...    28  4.8
TC89850 similar to GP|22748338|gb|AAN05340.1 Unknown protein {Or...    28  6.3
TC84344                                                                28  6.3
TC92619 similar to PIR|T47763|T47763 hypothetical protein F24I3....    28  6.3
TC87324 homologue to PIR|E86217|E86217 protein T27G7.10 [importe...    28  8.2
TC88376 similar to GP|8778315|gb|AAF79324.1| F14J16.29 {Arabidop...    28  8.2

>TC79535 weakly similar to GP|10178054|dbj|BAB11418.
           gene_id:MUB3.3~pir||T03813~similar to unknown protein
           {Arabidopsis thaliana}, partial (86%)
          Length = 1356

 Score = 85.9 bits (211), Expect = 3e-17
 Identities = 75/286 (26%), Positives = 132/286 (45%), Gaps = 37/286 (12%)
 Frame = +2

Query: 143 ESHHLSLSLPMNVSYNGLKHIIVGKGITVEVRRAREISFYYQSDLDLQRNGSVICSNQKN 202
           ++  + LSLP +V    LK +++  G  V V+ AR +S  +   L L  N S      +N
Sbjct: 446 DAKDMRLSLPHDVDAGVLKKVVLADGAVVTVKGARSVSLRHPLTLPLPLNRS------QN 607

Query: 203 EFWPFLQSMC-----------VPLIPIRIIGSASL----IAYVARNPYVQI-----GTAL 242
            F   L ++             PL+ +RI+G  SL     A  + N  +++     G   
Sbjct: 608 GFAAGLLTLAEHLRHASRGQDAPLLSLRIVGPTSLEAPSSASTSSNNRLKLKRLAPGLVE 787

Query: 243 ISEDAVELLPEKCYHGCVFRKQA------------CPVASLN---LRLILLEKILRSLLG 287
           +S  +   L +        +++A             P+ASLN     L+  E++L S+LG
Sbjct: 788 LSSQSKSKLVDTSLSTVDLQEEAPTLLTPTQFTALWPLASLNGSNANLLGFERLLSSVLG 967

Query: 288 HKILQDRLSGLIKANIKAYAGVKFPLELERDV--GNNATLSTLPDWRTRPSVERVWFEVM 345
            K  +     L+KA++ A   VK   + E+ +  G+  +    P+WRT+P   R+ FEV+
Sbjct: 968 PKANEKGSFRLLKADVSAQTFVKIGFQAEKKLKEGDGISFEGFPEWRTKPDTVRLHFEVL 1147

Query: 346 ARVEDSRLKPLSIKKVKPFIESDSVSWANLMSNLSYTKLRPVLLPP 391
           A+V+  ++ P  + +V P +  DSV+  N+++N       P++ PP
Sbjct: 1148AKVDGDKVIPERVMQVNPVVTEDSVA-PNMLTNNGTMSKMPLVQPP 1282


>BG648384 similar to GP|14718995|gb ceramide glucosyltransferase {Gossypium
           arboreum}, partial (26%)
          Length = 784

 Score = 29.3 bits (64), Expect = 2.8
 Identities = 11/19 (57%), Positives = 15/19 (78%)
 Frame = +2

Query: 135 GPFELRVHESHHLSLSLPM 153
           GP ELR+H   H++LSLP+
Sbjct: 113 GPVELRIHPLPHINLSLPL 169


>TC80121 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precursor (EC
           3.1.1.11) (Pectin methylesterase 3) (PE 3)., partial
           (5%)
          Length = 792

 Score = 29.3 bits (64), Expect = 2.8
 Identities = 18/54 (33%), Positives = 28/54 (51%)
 Frame = -3

Query: 34  SSTHSNITHIFQDILKAISSRQKWDLNDVRVFNFDVAKIRFGTSQNYLFRIGSS 87
           SST    T++FQ I   +  RQ+     + + NF + + R+GT  N+  RI  S
Sbjct: 616 SSTFQRNTNLFQPINTRLLIRQRSTNPRLNIINFTICQ-RYGTVSNHRHRIVES 458


>AW585898 weakly similar to GP|15983422|gb| At2g19880/F6F22.9 {Arabidopsis
           thaliana}, partial (12%)
          Length = 622

 Score = 29.3 bits (64), Expect = 2.8
 Identities = 11/19 (57%), Positives = 15/19 (78%)
 Frame = +1

Query: 135 GPFELRVHESHHLSLSLPM 153
           GP ELR+H   H++LSLP+
Sbjct: 253 GPVELRIHPLPHINLSLPL 309


>TC86336 similar to GP|14626761|gb|AAK71662.1 mature anther-specific
          protein LAT61 {Lycopersicon esculentum}, partial (37%)
          Length = 778

 Score = 28.9 bits (63), Expect = 3.7
 Identities = 15/29 (51%), Positives = 17/29 (57%)
 Frame = +1

Query: 14 FFIIFFLQLFTAFSNASSSSSSTHSNITH 42
          FF IF   L T F+   SSS S HSN +H
Sbjct: 25 FFFIFSSSLLTNFNIIFSSSFSRHSNNSH 111


>TC86335 similar to GP|14626761|gb|AAK71662.1 mature anther-specific
          protein LAT61 {Lycopersicon esculentum}, partial (60%)
          Length = 1595

 Score = 28.9 bits (63), Expect = 3.7
 Identities = 15/29 (51%), Positives = 17/29 (57%)
 Frame = +2

Query: 14 FFIIFFLQLFTAFSNASSSSSSTHSNITH 42
          FF IF   L T F+   SSS S HSN +H
Sbjct: 41 FFFIFSSSLLTNFNIIFSSSFSRHSNNSH 127


>TC77680 similar to GP|18650600|gb|AAL75900.1 AT4g38220/F20D10_340
           {Arabidopsis thaliana}, partial (86%)
          Length = 1433

 Score = 28.5 bits (62), Expect = 4.8
 Identities = 17/43 (39%), Positives = 24/43 (55%)
 Frame = +2

Query: 7   LRLHQLPFFIIFFLQLFTAFSNASSSSSSTHSNITHIFQDILK 49
           ++L++   F+I FL        +SSSSSS  SNI   FQ  L+
Sbjct: 59  MKLNRGSIFLIAFLLSTLTSLQSSSSSSSEESNIISRFQQYLQ 187


>TC78429 similar to GP|16604607|gb|AAL24096.1 putative AtBgamma protein
           {Arabidopsis thaliana}, partial (62%)
          Length = 1596

 Score = 28.5 bits (62), Expect = 4.8
 Identities = 16/29 (55%), Positives = 20/29 (68%), Gaps = 3/29 (10%)
 Frame = +3

Query: 15  FIIFFLQLFTAF---SNASSSSSSTHSNI 40
           F+ FFL LFT      N+SSSSSS+ S+I
Sbjct: 186 FLFFFLSLFTTSPPNPNSSSSSSSSSSSI 272


>TC89850 similar to GP|22748338|gb|AAN05340.1 Unknown protein {Oryza sativa
           (japonica cultivar-group)}, partial (34%)
          Length = 1093

 Score = 28.1 bits (61), Expect = 6.3
 Identities = 12/37 (32%), Positives = 20/37 (53%)
 Frame = +1

Query: 15  FIIFFLQLFTAFSNASSSSSSTHSNITHIFQDILKAI 51
           FI+FF Q+F A    S+ S +   +  H+   ++K I
Sbjct: 766 FIVFFNQIFDALCKLSADSDANVQSAAHLLDRLVKDI 876


>TC84344 
          Length = 769

 Score = 28.1 bits (61), Expect = 6.3
 Identities = 14/52 (26%), Positives = 25/52 (47%), Gaps = 2/52 (3%)
 Frame = +3

Query: 65  FNFDVAKIRFGTSQNYLFRIGSSKNNFTVKFSDEISSWNHN--KFTTTPKPD 114
           F+F ++  RF T+  + F +    NNF       + ++N+   +F T   PD
Sbjct: 177 FSFPISLTRFCTTTTHPFAVSYLVNNFGFSTESTLKAFNNKQVRFNTPDNPD 332


>TC92619 similar to PIR|T47763|T47763 hypothetical protein F24I3.110 -
          Arabidopsis thaliana, partial (43%)
          Length = 682

 Score = 28.1 bits (61), Expect = 6.3
 Identities = 15/27 (55%), Positives = 18/27 (66%)
 Frame = +3

Query: 18 FFLQLFTAFSNASSSSSSTHSNITHIF 44
          FFLQLF+ +S  SSS  STH  + H F
Sbjct: 60 FFLQLFS-YSPPSSSPYSTHKTLFHQF 137


>TC87324 homologue to PIR|E86217|E86217 protein T27G7.10 [imported] -
           Arabidopsis thaliana, partial (37%)
          Length = 1897

 Score = 27.7 bits (60), Expect = 8.2
 Identities = 19/59 (32%), Positives = 27/59 (45%), Gaps = 6/59 (10%)
 Frame = +2

Query: 114 DLASLVDQLSS------IAFLDYIKLEGPFELRVHESHHLSLSLPMNVSYNGLKHIIVG 166
           DL  L D+  S      IA++DY+ L    +   H    +SL L + V Y    H+I G
Sbjct: 425 DLMRLFDEYGSPSTAGDIAYIDYLFLGDYVDRGQHSLETISLLLALKVEYPNNIHLIRG 601


>TC88376 similar to GP|8778315|gb|AAF79324.1| F14J16.29 {Arabidopsis
          thaliana}, partial (25%)
          Length = 706

 Score = 27.7 bits (60), Expect = 8.2
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = +3

Query: 20 LQLFTAFSNASSSSSSTHSNITHIF 44
          LQ++T  S+ +SSS   + NI HIF
Sbjct: 6  LQIYTLISSINSSSHGLNENIHHIF 80


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.322    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,195,127
Number of Sequences: 36976
Number of extensions: 192431
Number of successful extensions: 1402
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1357
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1387
length of query: 400
length of database: 9,014,727
effective HSP length: 98
effective length of query: 302
effective length of database: 5,391,079
effective search space: 1628105858
effective search space used: 1628105858
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)


Medicago: description of AC148404.10