Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC148347.8 - phase: 0 /pseudo
         (97 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BG454068 weakly similar to GP|22202804|dbj hypothetical protein~...    80  1e-16
BG453710 weakly similar to GP|21537057|gb| unknown {Arabidopsis ...    56  2e-09
CA917035 similar to GP|7303305|gb|A CG6061-PA {Drosophila melano...    38  6e-04
BF633515                                                               35  0.004
TC91570 weakly similar to GP|7208782|emb|CAB76913.1 hypothetical...    28  0.76
AW775288 similar to GP|4140398|gb| sterol-C5(6)-desaturase {Nico...    26  2.2
TC87651 similar to GP|15292729|gb|AAK92733.1 putative polygalact...    26  2.9
TC83461 similar to PIR|T04778|T04778 hypothetical protein F10M10...    25  3.8
TC78641                                                                25  4.9
AW980950                                                               25  4.9
BQ752015 homologue to GP|18376006|em probable ATP citrate lyase ...    25  6.4
TC90002 weakly similar to GP|9757850|dbj|BAB08484.1 dimethylanil...    25  6.4
CA859602                                                               25  6.4
TC77848 similar to PIR|F86296|F86296 hypothetical protein AAF185...    25  6.4
BI268545                                                               24  8.4
TC86766 weakly similar to PIR|T06291|T06291 extensin homolog T9E...    24  8.4
TC78549 similar to GP|15027268|emb|CAC44902. replication licensi...    24  8.4
CB066686 similar to GP|14334416|gb| unknown protein {Arabidopsis...    24  8.4
AL377183 GP|2598587|emb cycloartenol synthase {Medicago truncatu...    24  8.4
TC86580 similar to GP|6513932|gb|AAF14836.1| unknown protein {Ar...    24  8.4

>BG454068 weakly similar to GP|22202804|dbj hypothetical protein~predicted by
           GeneMark.hmm etc. {Oryza sativa (japonica
           cultivar-group)}, partial (5%)
          Length = 673

 Score = 80.1 bits (196), Expect = 1e-16
 Identities = 39/93 (41%), Positives = 57/93 (60%)
 Frame = +3

Query: 5   WVKDVMRVSGLQWLQHVSYLVIDQGLLSVIAERWHEVTSSFHLPIWEMIVTLDDLSCLLH 64
           W  +V+R SGL  L   +Y  +D GLL   + RWH  TSSFHLP+ EM + LDD+SCLLH
Sbjct: 261 WFWNVIRASGLYPLLETNYGQVDHGLLIAFSLRWHSETSSFHLPVGEMTIALDDISCLLH 440

Query: 65  LSIESHMLYHNIYLTRSEAVDLMVELLGSDSGE 97
           + +  ++L+H   L+  +  + +V  LG +  E
Sbjct: 441 IPVGGNLLFHE-SLSIHQGTEYLVNYLGLEFEE 536


>BG453710 weakly similar to GP|21537057|gb| unknown {Arabidopsis thaliana},
           partial (6%)
          Length = 651

 Score = 56.2 bits (134), Expect = 2e-09
 Identities = 25/49 (51%), Positives = 33/49 (67%)
 Frame = -1

Query: 27  DQGLLSVIAERWHEVTSSFHLPIWEMIVTLDDLSCLLHLSIESHMLYHN 75
           D  ++  + ERWH  TSSFHLP+ EM +TLDD+  LLH+ I   ML H+
Sbjct: 474 DPSMIRAMCERWHTETSSFHLPMGEMTITLDDVYNLLHIPIHGCMLDHD 328


>CA917035 similar to GP|7303305|gb|A CG6061-PA {Drosophila melanogaster},
           partial (2%)
          Length = 365

 Score = 38.1 bits (87), Expect = 6e-04
 Identities = 17/39 (43%), Positives = 23/39 (58%)
 Frame = +2

Query: 8   DVMRVSGLQWLQHVSYLVIDQGLLSVIAERWHEVTSSFH 46
           +V+  SGL  L   +Y  +D  LL   ++RWH  TSSFH
Sbjct: 248 NVIMASGLYLLLETNYGQVDHSLLIAFSDRWHSETSSFH 364


>BF633515 
          Length = 249

 Score = 35.4 bits (80), Expect = 0.004
 Identities = 17/42 (40%), Positives = 23/42 (54%)
 Frame = +2

Query: 56 LDDLSCLLHLSIESHMLYHNIYLTRSEAVDLMVELLGSDSGE 97
          LDD++CL HL IE  ML H   + + E   L++  LG    E
Sbjct: 2  LDDVACLTHLPIEGRMLSHGKKMPKHEGAALLMRHLGVSQQE 127


>TC91570 weakly similar to GP|7208782|emb|CAB76913.1 hypothetical protein
           {Cicer arietinum}, partial (17%)
          Length = 1446

 Score = 27.7 bits (60), Expect = 0.76
 Identities = 17/55 (30%), Positives = 26/55 (46%)
 Frame = -2

Query: 20  HVSYLVIDQGLLSVIAERWHEVTSSFHLPIWEMIVTLDDLSCLLHLSIESHMLYH 74
           H S L +    LS++   WH   SS     + ++  + DLS L H  + S + YH
Sbjct: 584 HYSLLALSVQDLSLL---WHRHLSSSLAYHYSLVFFVQDLSLL*HRHLSSSLAYH 429


>AW775288 similar to GP|4140398|gb| sterol-C5(6)-desaturase {Nicotiana
           tabacum}, partial (73%)
          Length = 716

 Score = 26.2 bits (56), Expect = 2.2
 Identities = 8/22 (36%), Positives = 13/22 (58%)
 Frame = +2

Query: 5   WVKDVMRVSGLQWLQHVSYLVI 26
           W K   R++ L W+ ++ YL I
Sbjct: 422 WTKSFPRINNLGWIAYIGYLAI 487


>TC87651 similar to GP|15292729|gb|AAK92733.1 putative polygalacturonase PG1
           {Arabidopsis thaliana}, partial (35%)
          Length = 822

 Score = 25.8 bits (55), Expect = 2.9
 Identities = 24/99 (24%), Positives = 39/99 (39%), Gaps = 11/99 (11%)
 Frame = +1

Query: 10  MRVSGLQWLQHVSYLVIDQGLLSVIAERWHEVTS---------SFHLP--IWEMIVTLDD 58
           +++    W+  + YL     L  +I E W E T+          F LP  +   ++TL  
Sbjct: 154 IQILNFSWISFMDYLFFGLPLYYLI*EMWMEGTTFTPSKRKYLPFFLPTLLLVFLLTLTQ 333

Query: 59  LSCLLHLSIESHMLYHNIYLTRSEAVDLMVELLGSDSGE 97
           +   + L     ML+H   L + E V  M  L     G+
Sbjct: 334 MILAIRLRTVFSMLHH---LEQLEMVQQMTHLHSRKHGK 441


>TC83461 similar to PIR|T04778|T04778 hypothetical protein F10M10.90 -
           Arabidopsis thaliana, partial (25%)
          Length = 663

 Score = 25.4 bits (54), Expect = 3.8
 Identities = 15/43 (34%), Positives = 24/43 (54%), Gaps = 3/43 (6%)
 Frame = -2

Query: 41  VTSSFHLP-IWEMIVTLDDLSCLLHL--SIESHMLYHNIYLTR 80
           VTS   +P + + + T      +LHL   I +H L+H++ LTR
Sbjct: 653 VTSILKIPKLLQCLYTSPPFKPILHLFLKISNHRLHHHLILTR 525


>TC78641 
          Length = 550

 Score = 25.0 bits (53), Expect = 4.9
 Identities = 9/27 (33%), Positives = 18/27 (66%)
 Frame = +1

Query: 30  LLSVIAERWHEVTSSFHLPIWEMIVTL 56
           +++VI+ +W  +  SF LP+W +  T+
Sbjct: 367 VIAVISNKWIYIYFSFPLPLWLLY*TI 447


>AW980950 
          Length = 548

 Score = 25.0 bits (53), Expect = 4.9
 Identities = 9/27 (33%), Positives = 18/27 (66%)
 Frame = +1

Query: 30  LLSVIAERWHEVTSSFHLPIWEMIVTL 56
           +++VI+ +W  +  SF LP+W +  T+
Sbjct: 430 VIAVISNKWIYIYFSFPLPLWLLY*TI 510


>BQ752015 homologue to GP|18376006|em probable ATP citrate lyase subunit 1
           {Neurospora crassa}, partial (23%)
          Length = 523

 Score = 24.6 bits (52), Expect = 6.4
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = -2

Query: 6   VKDVMRVSGLQWLQHVSYLVIDQGLLSVIAER 37
           V+  +  +GLQ +   S LV++QG+L   A+R
Sbjct: 234 VEHALDGAGLQTVDEASGLVVEQGVLGKRAQR 139


>TC90002 weakly similar to GP|9757850|dbj|BAB08484.1 dimethylaniline
           monooxygenase (N-oxide-forming)-like protein
           {Arabidopsis thaliana}, partial (26%)
          Length = 778

 Score = 24.6 bits (52), Expect = 6.4
 Identities = 15/47 (31%), Positives = 23/47 (48%)
 Frame = -2

Query: 2   QEQWVKDVMRVSGLQWLQHVSYLVIDQGLLSVIAERWHEVTSSFHLP 48
           +E+ V  V +   LQWL    +L  + G+ ++  E WH      HLP
Sbjct: 234 EEKRVCIVAQHDYLQWLLFHLFLGKECGICTLYNELWHLHR*RIHLP 94


>CA859602 
          Length = 587

 Score = 24.6 bits (52), Expect = 6.4
 Identities = 10/33 (30%), Positives = 22/33 (66%)
 Frame = -1

Query: 45  FHLPIWEMIVTLDDLSCLLHLSIESHMLYHNIY 77
           F LP++ ++  ++ L+ LLH+ + +  + +NIY
Sbjct: 317 FILPLYNLLNDINVLNLLLHIFVYTLNI*YNIY 219


>TC77848 similar to PIR|F86296|F86296 hypothetical protein AAF18512.1
           [imported] - Arabidopsis thaliana, complete
          Length = 1592

 Score = 24.6 bits (52), Expect = 6.4
 Identities = 11/52 (21%), Positives = 24/52 (46%)
 Frame = +2

Query: 23  YLVIDQGLLSVIAERWHEVTSSFHLPIWEMIVTLDDLSCLLHLSIESHMLYH 74
           +L++   LL     RW++    +    W + + +  L C +   + S +L+H
Sbjct: 593 FLLVQVVLLLDFVHRWNDTWVGYDEQFWYIALFVVSLVCYVATFVFSGVLFH 748


>BI268545 
          Length = 467

 Score = 24.3 bits (51), Expect = 8.4
 Identities = 13/39 (33%), Positives = 20/39 (50%)
 Frame = -1

Query: 18  LQHVSYLVIDQGLLSVIAERWHEVTSSFHLPIWEMIVTL 56
           + H SY   D  ++ +I +  H +   FHLPI+E    L
Sbjct: 290 IDHRSY---DMFIIQIIEK*RHCLEKHFHLPIYESFAYL 183


>TC86766 weakly similar to PIR|T06291|T06291 extensin homolog T9E8.80 -
           Arabidopsis thaliana, partial (18%)
          Length = 1260

 Score = 24.3 bits (51), Expect = 8.4
 Identities = 9/14 (64%), Positives = 12/14 (85%)
 Frame = +3

Query: 62  LLHLSIESHMLYHN 75
           L HLS+ESHM +H+
Sbjct: 537 LYHLSLESHMHHHH 578


>TC78549 similar to GP|15027268|emb|CAC44902. replication licensing factor
            MCM7 homologue {Zea mays}, partial (73%)
          Length = 1900

 Score = 24.3 bits (51), Expect = 8.4
 Identities = 6/15 (40%), Positives = 13/15 (86%)
 Frame = -2

Query: 61   CLLHLSIESHMLYHN 75
            CL+H+++ + +L+HN
Sbjct: 1434 CLIHITLSNSLLFHN 1390


>CB066686 similar to GP|14334416|gb| unknown protein {Arabidopsis thaliana},
           partial (4%)
          Length = 306

 Score = 24.3 bits (51), Expect = 8.4
 Identities = 13/36 (36%), Positives = 20/36 (55%)
 Frame = +1

Query: 30  LLSVIAERWHEVTSSFHLPIWEMIVTLDDLSCLLHL 65
           LLS++ E+      SF   +WE+I+ L  L   LH+
Sbjct: 166 LLSLVREK----NFSFKFQVWEIIIELFLLRSYLHI 261


>AL377183 GP|2598587|emb cycloartenol synthase {Medicago truncatula}, partial
           (12%)
          Length = 370

 Score = 24.3 bits (51), Expect = 8.4
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = +1

Query: 39  HEVTSSFHLPIWEMIVTLDDLSCLLHLSIE 68
           H + +SF   +WE IVT   L  L  L +E
Sbjct: 88  HHIETSFPYGLWESIVTKSCLHKLQMLHLE 177


>TC86580 similar to GP|6513932|gb|AAF14836.1| unknown protein {Arabidopsis
           thaliana}, partial (83%)
          Length = 2108

 Score = 24.3 bits (51), Expect = 8.4
 Identities = 10/23 (43%), Positives = 14/23 (60%)
 Frame = +2

Query: 39  HEVTSSFHLPIWEMIVTLDDLSC 61
           H+  S +HLP    I++L  LSC
Sbjct: 101 HKTDSKYHLPTSLFILSLSFLSC 169


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.323    0.136    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,715,714
Number of Sequences: 36976
Number of extensions: 53475
Number of successful extensions: 348
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 343
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 347
length of query: 97
length of database: 9,014,727
effective HSP length: 73
effective length of query: 24
effective length of database: 6,315,479
effective search space: 151571496
effective search space used: 151571496
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 50 (23.9 bits)


Medicago: description of AC148347.8