BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148347.5 - phase: 0
(118 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ148646 weakly similar to GP|11596186|gb| cystatin-like protein... 95 4e-21
TC86653 weakly similar to GP|21592732|gb|AAM64681.1 cysteine pro... 87 1e-18
TC89955 similar to GP|22901842|gb|AAN10097.1 NADH dehydrogenase ... 65 5e-12
TC86465 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhi... 41 6e-05
TC77929 similar to PIR|T07139|T07139 cysteine proteinase inhibit... 40 1e-04
BI310700 weakly similar to GP|21592712|gb| cystatin-like protein... 37 0.001
TC87034 homologue to SP|P13983|EXTN_TOBAC Extensin precursor (Ce... 37 0.001
TC77459 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhi... 34 0.007
BI311369 similar to GP|11596186|gb| cystatin-like protein {Citru... 33 0.016
BQ150746 homologue to GP|10281475|gb| mutant green fluorescent p... 28 0.39
TC77149 similar to GP|3643249|gb|AAC36740.1| thaumatin-like prot... 27 1.1
TC76714 similar to SP|P41127|RL13_ARATH 60S ribosomal protein L1... 27 1.1
AJ502739 similar to GP|10119914|gb brain otoferlin {Mus musculus... 27 1.1
TC85417 similar to SP|Q39211|RP3A_ARATH DNA-directed RNA polymer... 26 1.9
BQ141665 similar to GP|18568267|gb putative polyprotein {Zea may... 26 2.5
TC91128 similar to GP|5031281|gb|AAD38146.1| unknown {Prunus arm... 25 4.3
TC91842 similar to GP|21427461|gb|AAM53243.1 actin-related prote... 25 4.3
TC87313 weakly similar to PIR|S70769|S70769 S-receptor kinase (E... 25 5.7
TC87312 similar to GP|9759225|dbj|BAB09637.1 contains similarity... 25 5.7
AJ503375 similar to PIR|B86447|B864 hypothetical protein AAF8132... 25 5.7
>BQ148646 weakly similar to GP|11596186|gb| cystatin-like protein {Citrus x
paradisi}, partial (34%)
Length = 645
Score = 94.7 bits (234), Expect = 4e-21
Identities = 61/116 (52%), Positives = 77/116 (65%)
Frame = +2
Query: 1 MRLESMVLVLVVLLAFTATKQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEK 60
MR +S+V VL+VLLA+ A QAI G SPI NINDP VI++ANFAV E++ +K
Sbjct: 8 MRFQSLVPVLLVLLAYVARIQAIEDG-YSPIGNINDPHVIEIANFAVTEFD---KKSGAT 175
Query: 61 LRLWKVIKGESQIVADGVNYRLTLSATKVYTSNTYEAIVLEWSLQHLRNLTSFKLI 116
++ K+IKGESQIV G NYRLT SA + YEAIV E + R LTSF+L+
Sbjct: 176 IKFEKIIKGESQIV-HGTNYRLTFSAKIDSLTKVYEAIVYE--NKSTRKLTSFRLV 334
>TC86653 weakly similar to GP|21592732|gb|AAM64681.1 cysteine proteinase
inhibitor like protein {Arabidopsis thaliana}, partial
(64%)
Length = 651
Score = 86.7 bits (213), Expect = 1e-18
Identities = 52/110 (47%), Positives = 71/110 (64%), Gaps = 2/110 (1%)
Frame = +2
Query: 7 VLVLVVLLAFTAT--KQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLW 64
VL L V+ FT + K A+ +G +PI N+ DP+V+++A FAV EY ++ KL
Sbjct: 188 VLFLSVVSVFTVSDGKDAL-VGGWTPIKNVKDPQVVEIAKFAVTEYG---KQSGSKLSFV 355
Query: 65 KVIKGESQIVADGVNYRLTLSATKVYTSNTYEAIVLEWSLQHLRNLTSFK 114
KVIKGE+Q+V+ G NYRL L+A V + YEA+V E HL+NLTSFK
Sbjct: 356 KVIKGETQVVS-GTNYRLVLAAKVVSATKNYEALVWEKPWLHLKNLTSFK 502
>TC89955 similar to GP|22901842|gb|AAN10097.1 NADH dehydrogenase subunit 1
{Acryllium vulturinum}, partial (4%)
Length = 529
Score = 64.7 bits (156), Expect = 5e-12
Identities = 43/115 (37%), Positives = 66/115 (57%)
Frame = +1
Query: 1 MRLESMVLVLVVLLAFTATKQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEK 60
MR +S+ LVL+VL A AT QA+ N++ N++DP++ID+A F V E+N ++ E K
Sbjct: 55 MRFQSLFLVLIVLFASVATNQAL---NINRRVNVSDPQMIDIAKFTVTEHN--KQVTEAK 219
Query: 61 LRLWKVIKGESQIVADGVNYRLTLSATKVYTSNTYEAIVLEWSLQHLRNLTSFKL 115
L+ K++ G + D Y L +SA SN Y+++V E H NL F +
Sbjct: 220 LKFEKLLGG----LTDETVYILAISANNGSASNIYDSLVYESPPNHF-NLKYFHI 369
>TC86465 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhibitor
(Cystatin). [Cowpea] {Vigna unguiculata}, complete
Length = 660
Score = 41.2 bits (95), Expect = 6e-05
Identities = 28/94 (29%), Positives = 47/94 (49%), Gaps = 1/94 (1%)
Frame = +1
Query: 25 IGNLSPINNINDPKVID-VANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLT 83
+G + ++ + ID +A FAV+E+N +K L +VI + Q+VA G + +T
Sbjct: 67 VGGVRDVSGNQNSLAIDGLARFAVEEHN---KKQNALLEFSRVISAKEQVVA-GTIHHIT 234
Query: 84 LSATKVYTSNTYEAIVLEWSLQHLRNLTSFKLIQ 117
L YEA V E S + + + FKL++
Sbjct: 235 LEVKDGVNKKVYEAKVWEKSWMNFKEVQEFKLVE 336
>TC77929 similar to PIR|T07139|T07139 cysteine proteinase inhibitor -
soybean, partial (80%)
Length = 965
Score = 40.0 bits (92), Expect = 1e-04
Identities = 32/101 (31%), Positives = 47/101 (45%), Gaps = 2/101 (1%)
Frame = +2
Query: 16 FTATKQAIPIGNL--SPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQI 73
F + A P+G + SP + N ++ +A FAV ++N K L +V+K Q+
Sbjct: 164 FDHAQMATPLGGIQDSPSSE-NSLEIESLARFAVDQHN---AKQNSLLEFARVVKAREQV 331
Query: 74 VADGVNYRLTLSATKVYTSNTYEAIVLEWSLQHLRNLTSFK 114
VA G + LTL A YEA V + + LT FK
Sbjct: 332 VA-GTLHHLTLEAIDAGEKKIYEAKVWVKPWMNFKELTEFK 451
>BI310700 weakly similar to GP|21592712|gb| cystatin-like protein
{Arabidopsis thaliana}, partial (26%)
Length = 792
Score = 36.6 bits (83), Expect = 0.001
Identities = 24/87 (27%), Positives = 50/87 (56%), Gaps = 5/87 (5%)
Frame = +2
Query: 5 SMVLVLVVLLAFTATKQAIPIGNLSPINNINDPKVI-DVANFAVKEYNNRRRKPE----E 59
++V+ ++ + TA+ + +G + I+++ K + ++ FAVKEYN ++ E
Sbjct: 116 TIVITTLLCILSTASCGRVIVGARTEISDVGTNKEVQELGKFAVKEYNYKQGLNNGGGGE 295
Query: 60 KLRLWKVIKGESQIVADGVNYRLTLSA 86
L+ +V++ E Q+V+ G+ Y L +SA
Sbjct: 296 GLKFVEVVEAEQQVVS-GMKYYLNISA 373
>TC87034 homologue to SP|P13983|EXTN_TOBAC Extensin precursor (Cell wall
hydroxyproline-rich glycoprotein). [Common tobacco],
partial (2%)
Length = 644
Score = 36.6 bits (83), Expect = 0.001
Identities = 24/87 (27%), Positives = 50/87 (56%), Gaps = 5/87 (5%)
Frame = -2
Query: 5 SMVLVLVVLLAFTATKQAIPIGNLSPINNINDPKVI-DVANFAVKEYNNRRRKPE----E 59
++V+ ++ + TA+ + +G + I+++ K + ++ FAVKEYN ++ E
Sbjct: 571 TIVITTLLCILSTASCGRVIVGARTEISDVGTNKEVQELGKFAVKEYNYKQGLNNGGGGE 392
Query: 60 KLRLWKVIKGESQIVADGVNYRLTLSA 86
L+ +V++ E Q+V+ G+ Y L +SA
Sbjct: 391 GLKFVEVVEAEQQVVS-GMKYYLNISA 314
>TC77459 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhibitor
(Cystatin). [Cowpea] {Vigna unguiculata}, partial (92%)
Length = 618
Score = 34.3 bits (77), Expect = 0.007
Identities = 24/82 (29%), Positives = 43/82 (52%)
Frame = +3
Query: 35 NDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLTLSATKVYTSNT 94
N ++ ++A FAV+E+N ++ E +R V+ + ++V+ G Y +TL
Sbjct: 114 NSVEIDNLAIFAVQEHNIKQNAVLEFVR---VLNAKKKVVS-GTLYDITLETKDGGKQKV 281
Query: 95 YEAIVLEWSLQHLRNLTSFKLI 116
YEA +LE + + + FKLI
Sbjct: 282 YEAKILEKPWLNFKEVQEFKLI 347
>BI311369 similar to GP|11596186|gb| cystatin-like protein {Citrus x
paradisi}, partial (17%)
Length = 283
Score = 33.1 bits (74), Expect = 0.016
Identities = 16/24 (66%), Positives = 20/24 (82%)
Frame = +1
Query: 61 LRLWKVIKGESQIVADGVNYRLTL 84
L+ KV+KGESQ+VA G NYRLT+
Sbjct: 16 LKFEKVVKGESQVVA-GTNYRLTI 84
>BQ150746 homologue to GP|10281475|gb| mutant green fluorescent protein
{Cloning vector pGreenscript A}, partial (3%)
Length = 907
Score = 28.5 bits (62), Expect = 0.39
Identities = 16/49 (32%), Positives = 25/49 (50%)
Frame = -2
Query: 9 VLVVLLAFTATKQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKP 57
+L V L KQ + S INN ND + ++NF +K+ +N+ P
Sbjct: 153 ILHVNLTRMLVKQTNLNNHSSQINNSNDDACLRLSNFLIKKISNKNCLP 7
>TC77149 similar to GP|3643249|gb|AAC36740.1| thaumatin-like protein
precursor Mdtl1 {Malus x domestica}, partial (88%)
Length = 976
Score = 26.9 bits (58), Expect = 1.1
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +1
Query: 95 YEAIVLEWSLQHLRNLTSFKLIQ 117
Y +I+L W QH N+++ KL++
Sbjct: 586 YRSILLSWKFQHRGNMSTHKLLR 654
>TC76714 similar to SP|P41127|RL13_ARATH 60S ribosomal protein L13 (BBC1
protein homolog). [Mouse-ear cress] {Arabidopsis
thaliana}, complete
Length = 966
Score = 26.9 bits (58), Expect = 1.1
Identities = 11/29 (37%), Positives = 18/29 (61%)
Frame = -1
Query: 29 SPINNINDPKVIDVANFAVKEYNNRRRKP 57
S INN ND + ++NF +K+ +N+ P
Sbjct: 831 SQINNSNDDACLRLSNFLIKKISNKNCLP 745
>AJ502739 similar to GP|10119914|gb brain otoferlin {Mus musculus}, partial
(1%)
Length = 478
Score = 26.9 bits (58), Expect = 1.1
Identities = 16/27 (59%), Positives = 19/27 (70%)
Frame = +2
Query: 58 EEKLRLWKVIKGESQIVADGVNYRLTL 84
E KL+L V GES+ A GVNYRL+L
Sbjct: 11 ERKLKLKVVRSGESKD-ASGVNYRLSL 88
>TC85417 similar to SP|Q39211|RP3A_ARATH DNA-directed RNA polymerase II 36
kDa polypeptide A (EC 2.7.7.6) (RNA polymerase II
subunit 3)., partial (64%)
Length = 855
Score = 26.2 bits (56), Expect = 1.9
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = +2
Query: 68 KGESQIVADGVNYRLTLSATKVYTSNTYEAIVLEWSLQHLRNLTS 112
+G +I+ +G +L LS T + T+ ++ + WSL HLR S
Sbjct: 596 RGSGRIMPNGHQLQLLLSCT----NQTFTSMRI*WSL*HLRRKRS 718
>BQ141665 similar to GP|18568267|gb putative polyprotein {Zea mays}, partial
(1%)
Length = 1195
Score = 25.8 bits (55), Expect = 2.5
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +2
Query: 49 EYNNRRRKPEEKLRLWKVIKGESQ 72
E N RR+K E+ R+W +GE++
Sbjct: 212 EQNQRRKKAAEERRIWDGERGENK 283
>TC91128 similar to GP|5031281|gb|AAD38146.1| unknown {Prunus armeniaca},
partial (64%)
Length = 747
Score = 25.0 bits (53), Expect = 4.3
Identities = 15/46 (32%), Positives = 23/46 (49%), Gaps = 5/46 (10%)
Frame = +2
Query: 78 VNYRLTLSATKVYTSNTYEAIVLEWSLQ-----HLRNLTSFKLIQA 118
+NY +T+S + +NT + I+ W L L+N F LI A
Sbjct: 50 INYLITISTGETVINNTAQGIIK*WILMTKQDAKLQNCPYFVLIIA 187
>TC91842 similar to GP|21427461|gb|AAM53243.1 actin-related protein 3
{Arabidopsis thaliana}, partial (78%)
Length = 1021
Score = 25.0 bits (53), Expect = 4.3
Identities = 8/22 (36%), Positives = 15/22 (67%)
Frame = +1
Query: 47 VKEYNNRRRKPEEKLRLWKVIK 68
VKEYN ++P + ++ W+ +K
Sbjct: 724 VKEYNKHDKEPAKYIKQWRGVK 789
>TC87313 weakly similar to PIR|S70769|S70769 S-receptor kinase (EC 2.7.1.-)
Ark1 precursor - Arabidopsis thaliana, partial (4%)
Length = 555
Score = 24.6 bits (52), Expect = 5.7
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = +2
Query: 96 EAIVLEWSLQHLRNLT 111
+ + LEW + HLRN T
Sbjct: 323 QEVSLEWEISHLRNFT 370
>TC87312 similar to GP|9759225|dbj|BAB09637.1 contains similarity to protein
kinase~gene_id:K19M22.13 {Arabidopsis thaliana}, partial
(43%)
Length = 1531
Score = 24.6 bits (52), Expect = 5.7
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = +1
Query: 96 EAIVLEWSLQHLRNLT 111
+ + LEW + HLRN T
Sbjct: 337 QEVSLEWEISHLRNFT 384
>AJ503375 similar to PIR|B86447|B864 hypothetical protein AAF81322.1
[imported] - Arabidopsis thaliana, partial (47%)
Length = 515
Score = 24.6 bits (52), Expect = 5.7
Identities = 14/49 (28%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Frame = +1
Query: 48 KEYNNRRRKPEE-KLRLWKVIKGESQIVADGVNYRLTLSATKVYTSNTY 95
++ NNRR+K E+ + LW++ G + Y+L L + S Y
Sbjct: 49 RKSNNRRKKVEDFSMVLWEITLGTAYF*GLKRTYKLALKIQRKVVSPKY 195
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.134 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,088,501
Number of Sequences: 36976
Number of extensions: 32204
Number of successful extensions: 211
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of query: 118
length of database: 9,014,727
effective HSP length: 94
effective length of query: 24
effective length of database: 5,538,983
effective search space: 132935592
effective search space used: 132935592
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)
Medicago: description of AC148347.5