BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148289.1 + phase: 0
(340 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80729 auxin efflux carrier protein [Medicago truncatula] 172 2e-43
TC83905 auxin efflux carrier protein [Medicago truncatula] 169 1e-42
TC91788 auxin efflux carrier protein [Medicago truncatula] 167 7e-42
NP565645 NP565645|AY115840.1|AAM55301.1 auxin efflux carrier pro... 164 5e-41
TC81304 auxin efflux carrier protein [Medicago truncatula] 146 1e-35
BQ143930 39 0.003
BQ751233 similar to GP|18307448|em probable low-affinity hexose ... 31 0.78
TC90276 weakly similar to GP|20259057|gb|AAM14244.1 unknown prot... 30 1.7
TC88208 similar to GP|10177672|dbj|BAB11032. glucosidase II alph... 30 1.7
TC86454 homologue to GP|4567091|gb|AAD23582.1| SNF-1-like serine... 30 1.7
TC81380 similar to GP|19310773|gb|AAL85117.1 unknown protein {Ar... 28 5.1
TC79118 similar to GP|16323188|gb|AAL15328.1 AT5g67320/K8K14_4 {... 28 6.6
BE124004 weakly similar to EGAD|38497|4011 tola protein {Pseudom... 28 6.6
BQ149447 28 6.6
TC86798 similar to GP|1311386|pdb|1CBG| Cyanogenic Beta-Glucosid... 27 8.7
TC80063 similar to GP|6513925|gb|AAF14829.1| unknown protein {Ar... 27 8.7
>TC80729 auxin efflux carrier protein [Medicago truncatula]
Length = 2366
Score = 172 bits (436), Expect = 2e-43
Identities = 80/155 (51%), Positives = 107/155 (68%)
Frame = +1
Query: 1 MIGWEDVYKVIVAVVPLYFALILGYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFEFTA 60
MI W D+Y V+ AVVPLY A+IL YGSVRWWKIF+ +QC IN+ V F +PL +F F +
Sbjct: 1 MITWHDLYTVLTAVVPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 180
Query: 61 HIDPFKMNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVVGIPM 120
+P+ MN+ FI ADTL K+I++ + +W ++ S W IT FSL TL N+LV+GIP+
Sbjct: 181 TNNPYAMNYRFIAADTLQKIIMLLALTIWTNFTANGSLEWMITIFSLSTLPNTLVMGIPL 360
Query: 121 VKPMYGPMGVDLVVQASVVQAIIWLTLLLFVLEFR 155
+ MYG L+VQ V+Q IIW TLLLF+ E+R
Sbjct: 361 LIAMYGEYSGTLMVQVVVLQCIIWYTLLLFLFEYR 465
Score = 159 bits (401), Expect = 2e-39
Identities = 74/150 (49%), Positives = 107/150 (71%), Gaps = 5/150 (3%)
Frame = +1
Query: 195 TSSRLPFLQLMKR-----VWLKLIANPNSYGCVIGISWAFISNRWNLELPSMVEGSILIM 249
T +P +M R VW KLI NPN+Y +IG+ W+ ++ RW++ +P ++E SI I+
Sbjct: 1462 TVKHMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLIWSLVAFRWHVHMPKIIEKSISIL 1641
Query: 250 SKAGTGTAMFSMGIFMALQEKVISCGPSLTVFGLVLKFIAGPAAMAISAFIVGLRGDVLR 309
S AG G AMFS+G+FMALQ K+I+CG S+ F + ++F+ GPA MA ++ VGLRG++LR
Sbjct: 1642 SDAGLGMAMFSLGLFMALQPKMIACGNSVASFAMAVRFLTGPAVMAAASIAVGLRGNLLR 1821
Query: 310 IAIIQAAVPQSITSFIFAKEYELHAEVLST 339
+AI+QAA+PQ I F+FAKEY +H +LST
Sbjct: 1822 VAIVQAALPQGIVPFVFAKEYNVHPAILST 1911
>TC83905 auxin efflux carrier protein [Medicago truncatula]
Length = 1869
Score = 169 bits (428), Expect = 1e-42
Identities = 80/155 (51%), Positives = 108/155 (69%)
Frame = +1
Query: 1 MIGWEDVYKVIVAVVPLYFALILGYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFEFTA 60
MI +D+Y V+ AVVPLY A+IL YGSVRWWKIF+ +QC IN+ V F +PL +F F +
Sbjct: 1 MITLKDLYTVLTAVVPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAVFAVPLLSFHFIS 180
Query: 61 HIDPFKMNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVVGIPM 120
+P++MNF FI ADTL K+I++ ++LW + + W IT FSL TL N+LV+GIP+
Sbjct: 181 TNNPYQMNFRFIAADTLQKIIMLVALSLWTLFTKNGNLEWMITIFSLSTLPNTLVMGIPL 360
Query: 121 VKPMYGPMGVDLVVQASVVQAIIWLTLLLFVLEFR 155
+ MYG L+VQ V+Q IIW TLLLF+ E+R
Sbjct: 361 LIAMYGDYSGSLMVQVVVLQCIIWYTLLLFLFEYR 465
Score = 123 bits (309), Expect = 9e-29
Identities = 64/142 (45%), Positives = 88/142 (61%), Gaps = 9/142 (6%)
Frame = +1
Query: 208 VWLKLIANPNSYGCVIG---------ISWAFISNRWNLELPSMVEGSILIMSKAGTGTAM 258
VW KLI NPN+Y +IG + ++ N + P S AG G AM
Sbjct: 1402 VWRKLIRNPNTYSSLIGLI*VPCCL*VGCSYA*NNRRNQFP--------YCSDAGLGMAM 1557
Query: 259 FSMGIFMALQEKVISCGPSLTVFGLVLKFIAGPAAMAISAFIVGLRGDVLRIAIIQAAVP 318
FS+G+FMALQ K+I+CG S+ F + ++F+ GPA MA ++ VGLRG +L +AI+QAA+P
Sbjct: 1558 FSLGLFMALQPKIIACGNSVASFAMAIRFLTGPAVMAAASIAVGLRGTLLHVAIVQAALP 1737
Query: 319 QSITSFIFAKEYELHAEVLSTA 340
Q I F+FAKEY +H +LSTA
Sbjct: 1738 QGIVPFVFAKEYNVHPAILSTA 1803
>TC91788 auxin efflux carrier protein [Medicago truncatula]
Length = 2076
Score = 167 bits (422), Expect = 7e-42
Identities = 77/156 (49%), Positives = 111/156 (70%), Gaps = 1/156 (0%)
Frame = +1
Query: 1 MIGWEDVYKVIVAVVPLYFALILGYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFEFTA 60
MI +D+Y V A+VPLY A+IL YGSVRWWKIFT +QC IN+ V F +PL +F F +
Sbjct: 1 MITGKDIYDVFAAIVPLYVAMILAYGSVRWWKIFTPDQCSGINRFVAVFAVPLLSFHFIS 180
Query: 61 HIDPFKMNFLFIGADTLSKLIIVAVIALWAKCS-SKVSYSWSITSFSLCTLTNSLVVGIP 119
DP+ MN+ F+ AD+L K++I+ + +W S ++ S W+IT FSL TL N+LV+GIP
Sbjct: 181 SNDPYAMNYHFLAADSLQKVVILGALFIWNTFSKNQDSLDWTITLFSLSTLPNTLVMGIP 360
Query: 120 MVKPMYGPMGVDLVVQASVVQAIIWLTLLLFVLEFR 155
+++ MYG +L+VQ V+Q++IW TL+LF+ E+R
Sbjct: 361 LLRAMYGDFSGNLMVQIVVLQSVIWYTLMLFLFEYR 468
Score = 159 bits (401), Expect = 2e-39
Identities = 78/171 (45%), Positives = 115/171 (66%)
Frame = +1
Query: 169 KASSISNVTCEGEESKDVEANNIVEYTSSRLPFLQLMKRVWLKLIANPNSYGCVIGISWA 228
K+ IS + E DV N + S + +M VW KLI NPN+Y +IG+ W+
Sbjct: 1303 KSPYISQKKMDLEGGGDVSKNTQMPPASVMTRLILIM--VWRKLIRNPNTYSSLIGLVWS 1476
Query: 229 FISNRWNLELPSMVEGSILIMSKAGTGTAMFSMGIFMALQEKVISCGPSLTVFGLVLKFI 288
+S R+++E+PS+V+GSI I+S AG G AMFS+G+FMALQ K+I+CG + F + ++F+
Sbjct: 1477 LVSFRFHIEMPSIVKGSISILSDAGLGMAMFSLGLFMALQPKLIACGKRVATFSMAVRFL 1656
Query: 289 AGPAAMAISAFIVGLRGDVLRIAIIQAAVPQSITSFIFAKEYELHAEVLST 339
GPA +A ++ +G+RG +L +AI+QAA+PQ I F+FAKEY LH +LST
Sbjct: 1657 TGPAVIAATSIAIGIRGVLLHVAIVQAALPQGIVPFVFAKEYNLHPNILST 1809
>NP565645 NP565645|AY115840.1|AAM55301.1 auxin efflux carrier protein [Medicago
truncatula]
Length = 1575
Score = 164 bits (415), Expect = 5e-41
Identities = 80/170 (47%), Positives = 116/170 (68%), Gaps = 5/170 (2%)
Frame = +1
Query: 175 NVTCEGEESKDVEANNIVEYTSSRLPFLQLMKR-----VWLKLIANPNSYGCVIGISWAF 229
NV+ E E +D E E + +P +M R VW KLI NPN+Y +IG++W+
Sbjct: 1000 NVSPEKVEGED-EHKRGEEGGAKTMPPASIMTRLILIMVWRKLIRNPNTYSSIIGLTWSL 1176
Query: 230 ISNRWNLELPSMVEGSILIMSKAGTGTAMFSMGIFMALQEKVISCGPSLTVFGLVLKFIA 289
IS RWN+E+P ++ SI I+S AG G AMFS+G+FMALQ ++I+CG + F + ++F+
Sbjct: 1177 ISFRWNIEMPVIIAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNRIAAFSMAIRFLT 1356
Query: 290 GPAAMAISAFIVGLRGDVLRIAIIQAAVPQSITSFIFAKEYELHAEVLST 339
GPA MA ++ +VGLRG +L +AI+QAA+PQ I F+FAKEY +H ++LST
Sbjct: 1357 GPAVMAAASIVVGLRGTLLNVAIVQAALPQGIVPFVFAKEYNVHPDILST 1506
Score = 163 bits (412), Expect = 1e-40
Identities = 78/156 (50%), Positives = 107/156 (68%), Gaps = 1/156 (0%)
Frame = +1
Query: 1 MIGWEDVYKVIVAVVPLYFALILGYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFEFTA 60
MI D Y V+ ++VPLY A+IL YGSV+WWKIF+ +QC IN+ V F +PL +F F A
Sbjct: 1 MITLTDFYHVMTSMVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIA 180
Query: 61 HIDPFKMNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVVGIPM 120
+P+KMN F+ ADTL K+I++ + +WA S + W+IT FS+ TL N+LV+GIP+
Sbjct: 181 SNNPYKMNTRFLVADTLQKIIVLLALTIWANVSKRGCLEWTITLFSISTLPNTLVMGIPL 360
Query: 121 VKPMY-GPMGVDLVVQASVVQAIIWLTLLLFVLEFR 155
+K MY G L+VQ V+Q IIW TL+LF+ EFR
Sbjct: 361 LKCMYGGEFSGSLMVQIVVLQCIIWYTLILFMFEFR 468
>TC81304 auxin efflux carrier protein [Medicago truncatula]
Length = 831
Score = 146 bits (368), Expect = 1e-35
Identities = 63/125 (50%), Positives = 97/125 (77%)
Frame = +3
Query: 215 NPNSYGCVIGISWAFISNRWNLELPSMVEGSILIMSKAGTGTAMFSMGIFMALQEKVISC 274
NPN+Y +IG++W+ +S R+++E+P+++ SI I+S AG G AMFS+G+FMALQ K+I+C
Sbjct: 6 NPNTYSSLIGLTWSLVSFRYHIEMPAIIAKSISILSDAGLGMAMFSLGLFMALQPKIIAC 185
Query: 275 GPSLTVFGLVLKFIAGPAAMAISAFIVGLRGDVLRIAIIQAAVPQSITSFIFAKEYELHA 334
G S+ F + ++F+ GPA MA ++F VGL+G + +AI+QAA+PQ I F+FAKEY +H
Sbjct: 186 GNSIAAFSMGVRFLVGPAVMAAASFAVGLKGVLFHVAIVQAALPQGIVPFVFAKEYNVHP 365
Query: 335 EVLST 339
++LST
Sbjct: 366 DILST 380
>BQ143930
Length = 502
Score = 38.9 bits (89), Expect = 0.003
Identities = 29/83 (34%), Positives = 39/83 (46%), Gaps = 3/83 (3%)
Frame = -1
Query: 239 PSMVEGSILIMSKAGTG---TAMFSMGIFMALQEKVISCGPSLTVFGLVLKFIAGPAAMA 295
PS EG L+ S+ G ++ FS G+ A + +CG S LV IAG A A
Sbjct: 382 PSGAEGLDLV-SRQSCGLMRSSFFSDGLAGAPSDSGCACGSSRVAIVLVASHIAGGACQA 206
Query: 296 ISAFIVGLRGDVLRIAIIQAAVP 318
A GL GD LR ++ +P
Sbjct: 205 PLATSAGLVGDSLRTGVLATIIP 137
>BQ751233 similar to GP|18307448|em probable low-affinity hexose transporter
HXT3 {Neurospora crassa}, partial (30%)
Length = 659
Score = 30.8 bits (68), Expect = 0.78
Identities = 21/91 (23%), Positives = 44/91 (48%), Gaps = 4/91 (4%)
Frame = +3
Query: 74 ADTLSKLIIVAVIALWAKCS--SKVSYSWSITSFSLCTLTNSLVVG-IPMVKPMYGPMGV 130
ADTL + + ++ A W+ ++S + F++ L N + +G + +V PMY
Sbjct: 369 ADTLGRRLTISAFAFWSCVGIVIEISSQSAWYQFAIGRLVNGVGIGALSVVVPMYQSEST 548
Query: 131 DLVVQASVVQAI-IWLTLLLFVLEFRRTGIE 160
+++ +V +++TL ++ E G E
Sbjct: 549 PAIIRGVIVSTYQLFITLGIWTAEMGHWGTE 641
>TC90276 weakly similar to GP|20259057|gb|AAM14244.1 unknown protein
{Arabidopsis thaliana}, partial (56%)
Length = 697
Score = 29.6 bits (65), Expect = 1.7
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +3
Query: 199 LPFLQLMKRVWLKLIANPNSYGCVI 223
+P+LQ MKR LKL+ N NS+ C++
Sbjct: 129 VPYLQEMKREVLKLLRN*NSFLCLV 203
>TC88208 similar to GP|10177672|dbj|BAB11032. glucosidase II alpha subunit
{Arabidopsis thaliana}, partial (34%)
Length = 1505
Score = 29.6 bits (65), Expect = 1.7
Identities = 11/38 (28%), Positives = 20/38 (51%)
Frame = -1
Query: 24 GYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFEFTAH 61
G S+ W+++ +++C + L C LP F+F H
Sbjct: 761 GPSSLEWFQLSGKQECSPL*HLTCVSPLPCTQFQFLDH 648
>TC86454 homologue to GP|4567091|gb|AAD23582.1| SNF-1-like serine/threonine
protein kinase {Glycine max}, partial (90%)
Length = 2194
Score = 29.6 bits (65), Expect = 1.7
Identities = 21/62 (33%), Positives = 29/62 (45%), Gaps = 2/62 (3%)
Frame = -3
Query: 216 PNSYGCVIGISWAFISNRW--NLELPSMVEGSILIMSKAGTGTAMFSMGIFMALQEKVIS 273
P+SY VI ISW +SN W N V+ I + K T A S +Q +++S
Sbjct: 1208 PHSYSLVINISWKAVSNDWRSNFTRVDTVQTRIHCLLKLSTEIA--SGDTITVVQ*QIVS 1035
Query: 274 CG 275
G
Sbjct: 1034 HG 1029
>TC81380 similar to GP|19310773|gb|AAL85117.1 unknown protein {Arabidopsis
thaliana}, partial (57%)
Length = 1640
Score = 28.1 bits (61), Expect = 5.1
Identities = 23/68 (33%), Positives = 31/68 (44%), Gaps = 2/68 (2%)
Frame = -2
Query: 47 CYFTLPLFAFEFTAHIDPFKMNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSFS 106
C L F F+ H D M+F +GA +LS LI +A CS++V+ S
Sbjct: 1555 CGVRLEAKQFTFSVHSD---MDF*IVGATSLSWLISTWKLAKCC*CSARVAAKDSAWPLE 1385
Query: 107 L--CTLTN 112
L C L N
Sbjct: 1384 LPSCALRN 1361
>TC79118 similar to GP|16323188|gb|AAL15328.1 AT5g67320/K8K14_4 {Arabidopsis
thaliana}, partial (44%)
Length = 1098
Score = 27.7 bits (60), Expect = 6.6
Identities = 31/127 (24%), Positives = 52/127 (40%), Gaps = 3/127 (2%)
Frame = -1
Query: 15 VPLYFALILGYGSVRWWKIFTREQCDAINK--LVCYFTLPLFAFEFTAHIDPFKMNFLFI 72
+PLY +I + F C +++ L Y L L A E+T + PFK
Sbjct: 720 LPLYVLII--------FPSFNDHMCKDLSRDPLAIYSPLGLNATEYTGCLCPFK------ 583
Query: 73 GADTLSKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLV-VGIPMVKPMYGPMGVD 131
LW S +S+S+++ S +N L+ + +P GP+G+
Sbjct: 582 ---------------LWMSFPSSISHSFTVESNEALANSNFLLGLFVP------GPVGLH 466
Query: 132 LVVQASV 138
L+V S+
Sbjct: 465 LMVYISL 445
>BE124004 weakly similar to EGAD|38497|4011 tola protein {Pseudomonas
aeruginosa}, partial (10%)
Length = 568
Score = 27.7 bits (60), Expect = 6.6
Identities = 13/39 (33%), Positives = 25/39 (63%)
Frame = -3
Query: 78 SKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVV 116
S+ +I + I++W C+S + +W +TSF + + NS +V
Sbjct: 284 SQELIKSSISIW-DCNSDLVLAWYLTSFRILSYENSSLV 171
>BQ149447
Length = 634
Score = 27.7 bits (60), Expect = 6.6
Identities = 13/39 (33%), Positives = 25/39 (63%)
Frame = -2
Query: 78 SKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVV 116
S+ +I + I++W C+S + +W +TSF + + NS +V
Sbjct: 234 SQELIKSSISIW-DCNSDLVLAWYLTSFRILSYENSSLV 121
>TC86798 similar to GP|1311386|pdb|1CBG| Cyanogenic Beta-Glucosidase Mol_id:
1; Molecule: Cyanogenic Beta-Glucosidase; Chain: Null;
Ec: 3.2., partial (97%)
Length = 1972
Score = 27.3 bits (59), Expect = 8.7
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = -1
Query: 89 WAKCSSKVSYSWSITSFSLCT 109
W S + Y+WS ++FS CT
Sbjct: 328 WVFMSKGIPYTWSFSTFSYCT 266
>TC80063 similar to GP|6513925|gb|AAF14829.1| unknown protein {Arabidopsis
thaliana}, partial (71%)
Length = 736
Score = 27.3 bits (59), Expect = 8.7
Identities = 18/59 (30%), Positives = 31/59 (52%)
Frame = +1
Query: 67 MNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVVGIPMVKPMY 125
M +F+ + L ++ IV+ + CS ++ TS S+ L NSL + +P + PMY
Sbjct: 481 MFMVFVNLEYL-EMEIVSSVHFQTSCSGHLN----TTSISVKIL*NSLKITVPYMNPMY 642
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.139 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,181,362
Number of Sequences: 36976
Number of extensions: 159285
Number of successful extensions: 1226
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 1214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1223
length of query: 340
length of database: 9,014,727
effective HSP length: 97
effective length of query: 243
effective length of database: 5,428,055
effective search space: 1319017365
effective search space used: 1319017365
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148289.1