BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147963.2 - phase: 0
(129 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC92307 weakly similar to PIR|E84434|E84434 probable phloem-spec... 72 8e-14
TC81135 weakly similar to GP|10177856|dbj|BAB11208. phloem-speci... 69 4e-13
TC80392 weakly similar to GP|15088626|gb|AAK84134.1 nictaba {Nic... 67 3e-12
TC79147 weakly similar to GP|10177856|dbj|BAB11208. phloem-speci... 65 6e-12
TC80561 weakly similar to GP|10177856|dbj|BAB11208. phloem-speci... 60 2e-10
TC79148 57 3e-09
TC84458 weakly similar to PIR|B96604|B96604 hypothetical protein... 52 6e-08
BG588872 weakly similar to GP|21592660|gb phloem-specific lectin... 32 0.069
BQ137192 29 0.58
TC81478 weakly similar to GP|21740720|emb|CAD40841. OSJNBa0086B1... 27 2.9
TC80459 weakly similar to GP|22831085|dbj|BAC15947. contains EST... 25 6.4
TC83771 25 6.4
BF003631 similar to GP|297616|emb|C immunoglobulin variable regi... 25 6.4
TC85449 homologue to PIR|S71923|S71923 cysteine proteinase (EC 3... 25 8.4
TC81064 similar to GP|15451210|gb|AAK96876.1 Unknown protein {Ar... 25 8.4
>TC92307 weakly similar to PIR|E84434|E84434 probable phloem-specific lectin
[imported] - Arabidopsis thaliana, partial (19%)
Length = 817
Score = 71.6 bits (174), Expect = 8e-14
Identities = 50/130 (38%), Positives = 74/130 (56%), Gaps = 6/130 (4%)
Frame = +1
Query: 3 THILTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYEDTMFYENH 62
T L+PNT YV YL+ K+ + YG + P E+SV + + + N
Sbjct: 451 TLALSPNTRYVTYLVFKMIN-AYGFEYFPVELSVGIEGGHSSTKIVC-------LADPNA 606
Query: 63 RKMERNKLM--EDNKEIRV--PSKRDDGWMEIELGEFF-CGEVDMEVKMSVMEV-GYRLK 116
R+ R++++ E N+ +R+ P+ R DGW+EIE+GEFF G D EV+MSV+++ G K
Sbjct: 607 RRRHRSRIIVTEPNRVLRLQRPNVRSDGWLEIEMGEFFISGLEDEEVQMSVIDIKGGHWK 786
Query: 117 GGLIVEGIEV 126
G VEGIEV
Sbjct: 787 RGFFVEGIEV 816
>TC81135 weakly similar to GP|10177856|dbj|BAB11208. phloem-specific
lectin-like protein {Arabidopsis thaliana}, partial
(32%)
Length = 1186
Score = 69.3 bits (168), Expect = 4e-13
Identities = 48/132 (36%), Positives = 74/132 (55%), Gaps = 3/132 (2%)
Frame = +2
Query: 1 MRTHILTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYEDTMFYE 60
+ T +L+PNT Y Y++ K+ + G + PA++SV V+ G + T
Sbjct: 533 INTIVLSPNTQYAAYVVFKMIN-ASGFQNRPADLSVG----VEGGQS-------STKIVC 676
Query: 61 NHRKMERNKLMEDNKE-IRVPSKRDDGWMEIELGEFF-CGEVDMEVKMSVMEV-GYRLKG 117
+E + D + ++ PS R DGW+EIE+GEFF G + EV+M+V++ G K
Sbjct: 677 LDPNVEGRPQLHDRVDGLQRPSVRSDGWLEIEMGEFFNSGIENEEVQMNVIQTKGGNWKR 856
Query: 118 GLIVEGIEVRPK 129
GL++EGIEVRPK
Sbjct: 857 GLVLEGIEVRPK 892
>TC80392 weakly similar to GP|15088626|gb|AAK84134.1 nictaba {Nicotiana
tabacum}, partial (29%)
Length = 1161
Score = 66.6 bits (161), Expect = 3e-12
Identities = 49/129 (37%), Positives = 66/129 (50%)
Frame = +2
Query: 1 MRTHILTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYEDTMFYE 60
+ T L+PNT Y YL+ K+ G + P E+SV V+ G + D
Sbjct: 230 INTLSLSPNTQYAAYLVFKMIE-AEGFQNCPIEISVG----VEGGQSGTKIDCLDP---- 382
Query: 61 NHRKMERNKLMEDNKEIRVPSKRDDGWMEIELGEFFCGEVDMEVKMSVMEVGYRLKGGLI 120
N + N+ + ++ PS R DGW+EIE+GEF G D +V M+V E K GL
Sbjct: 383 NVEGGQHNRAVG----LQRPSVRSDGWLEIEMGEFNSGLEDGDVLMNVKETN-NWKSGLF 547
Query: 121 VEGIEVRPK 129
VEGIEVRPK
Sbjct: 548 VEGIEVRPK 574
>TC79147 weakly similar to GP|10177856|dbj|BAB11208. phloem-specific
lectin-like protein {Arabidopsis thaliana}, partial
(32%)
Length = 958
Score = 65.5 bits (158), Expect = 6e-12
Identities = 43/125 (34%), Positives = 64/125 (50%), Gaps = 1/125 (0%)
Frame = +2
Query: 6 LTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYEDTMFYENHRKM 65
L+PNT Y YL+ K+ YG ++ P ++SV + + + + +N R
Sbjct: 473 LSPNTQYAAYLVFKLIDG-YGFETLPVDLSVGVEGGHSSTKIVCLDPNVERR--QNSRHA 643
Query: 66 ERNKLMEDNKEIRVPSKRDDGWMEIELGEFFCGEVDMEVKMSVMEV-GYRLKGGLIVEGI 124
+ + P R DGW+EIE+GEF G + EV+MSV+E+ KG +EGI
Sbjct: 644 RFYGRADRVVGLPRPGVRSDGWLEIEMGEFNSGLENEEVQMSVIEIKAGETKGNFFLEGI 823
Query: 125 EVRPK 129
EVRPK
Sbjct: 824 EVRPK 838
>TC80561 weakly similar to GP|10177856|dbj|BAB11208. phloem-specific
lectin-like protein {Arabidopsis thaliana}, partial
(52%)
Length = 1436
Score = 60.1 bits (144), Expect = 2e-10
Identities = 48/146 (32%), Positives = 71/146 (47%), Gaps = 19/146 (13%)
Frame = +3
Query: 3 THILTPNTTYVVYLITKVSHR-VYGLDSAPAEVSVAMA--NKVQNG----------MAYL 49
T +L+P T Y YL+ K S +G + P E S+ +A + V+ + Y
Sbjct: 606 TIMLSPKTLYGAYLVFKSSAAGAFGFEYQPCEASIDIAGGDTVERNVFLDAGRGRRLRYQ 785
Query: 50 YNKYEDTMFYENHRKMERNKLMEDNKEI----RVPSKRDDGWMEIELGEFFC-GEVDMEV 104
T + +E + + + P +R DGW+E+ELGEFF G D +V
Sbjct: 786 IVPRSRTTGILTRLRSPVEAPVEPTESVADLRKYPKERADGWLEMELGEFFNEGGDDKQV 965
Query: 105 KMSVMEV-GYRLKGGLIVEGIEVRPK 129
+ V EV G KGGL+V+GIE+RPK
Sbjct: 966 DIGVCEVKGGGWKGGLVVQGIEIRPK 1043
>TC79148
Length = 553
Score = 56.6 bits (135), Expect = 3e-09
Identities = 30/56 (53%), Positives = 37/56 (65%), Gaps = 3/56 (5%)
Frame = +1
Query: 77 IRVPSKRDDGWMEIELGEFF-CGEVDMEVKMSVMEV--GYRLKGGLIVEGIEVRPK 129
++ P R DGW+E E+GEFF G D EV+MS+ + GY K G VEGIEVRPK
Sbjct: 121 LQQPRMRSDGWLETEMGEFFNSGLEDEEVQMSITGIKDGYTWKRGFFVEGIEVRPK 288
>TC84458 weakly similar to PIR|B96604|B96604 hypothetical protein F14G9.14
[imported] - Arabidopsis thaliana, partial (24%)
Length = 766
Score = 52.0 bits (123), Expect = 6e-08
Identities = 42/131 (32%), Positives = 57/131 (43%), Gaps = 2/131 (1%)
Frame = +1
Query: 1 MRTHILTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYEDTMFYE 60
+ +L+P T Y YL K YGLD P +N +NG+ +K
Sbjct: 442 INNEMLSPKTMYGAYLKVKNVECAYGLDLLPC----IRSNCKRNGICNCEHK-------- 585
Query: 61 NHRKMERNKLMEDNKEIRVPSKRDDGWMEIELGEFFCGEVDM-EVKMSVMEV-GYRLKGG 118
D W+EIELG F+ +V + EV+M + EV G KG
Sbjct: 586 ------------------------DEWLEIELGSFYTEKVQVQEVRMCLKEVNGVDFKGR 693
Query: 119 LIVEGIEVRPK 129
LIV+GIE+RPK
Sbjct: 694 LIVDGIELRPK 726
>BG588872 weakly similar to GP|21592660|gb phloem-specific lectin PP2-like
protein {Arabidopsis thaliana}, partial (55%)
Length = 655
Score = 32.0 bits (71), Expect = 0.069
Identities = 21/75 (28%), Positives = 34/75 (45%)
Frame = +2
Query: 19 KVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYEDTMFYENHRKMERNKLMEDNKEIR 78
K++ R YGLDS P+EVS+ + N K ++ ++ + K N E
Sbjct: 467 KLADRAYGLDSLPSEVSIEVGN----------YKSQENVYIRSQSKRNGKAYCHCNCE-- 610
Query: 79 VPSKRDDGWMEIELG 93
+D W+EI+ G
Sbjct: 611 ----HNDEWLEIK*G 643
>BQ137192
Length = 1026
Score = 28.9 bits (63), Expect = 0.58
Identities = 13/42 (30%), Positives = 27/42 (63%), Gaps = 1/42 (2%)
Frame = +2
Query: 41 KVQNGMAYLYNKY-EDTMFYENHRKMERNKLMEDNKEIRVPS 81
+V M ++N++ EDT + R++ N+++ED + IR+P+
Sbjct: 524 QVTQQMTQMHNQHHEDTDTRQTTRRIRNNEILEDAESIRLPT 649
>TC81478 weakly similar to GP|21740720|emb|CAD40841. OSJNBa0086B14.13 {Oryza
sativa}, partial (57%)
Length = 1585
Score = 26.6 bits (57), Expect = 2.9
Identities = 15/45 (33%), Positives = 20/45 (44%), Gaps = 3/45 (6%)
Frame = +3
Query: 58 FYENHRKMERNKLMEDNKEIRVPSK---RDDGWMEIELGEFFCGE 99
F N R +KL+ + I P D WME+ GE+ C E
Sbjct: 1029 FERNFR*RTNSKLVSTREGIESPFNWWILDSLWMELNHGEYMCXE 1163
>TC80459 weakly similar to GP|22831085|dbj|BAC15947. contains EST
AU163066(S15063)~unknown protein {Oryza sativa (japonica
cultivar-group)}, partial (4%)
Length = 1116
Score = 25.4 bits (54), Expect = 6.4
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +2
Query: 58 FYENHRKMERNKLMEDNKEIRVPSKRDDGWMEI 90
FY+N +++ KL +N E+ SK D W+++
Sbjct: 317 FYDNGLAIKQFKLSVNNFELHYMSKDVDHWLKL 415
>TC83771
Length = 531
Score = 25.4 bits (54), Expect = 6.4
Identities = 10/17 (58%), Positives = 15/17 (87%)
Frame = -2
Query: 23 RVYGLDSAPAEVSVAMA 39
RV+GLD AP+E++V +A
Sbjct: 365 RVFGLDVAPSELTVLLA 315
>BF003631 similar to GP|297616|emb|C immunoglobulin variable region {Mus
musculus domesticus}, partial (16%)
Length = 436
Score = 25.4 bits (54), Expect = 6.4
Identities = 11/30 (36%), Positives = 19/30 (62%)
Frame = +3
Query: 9 NTTYVVYLITKVSHRVYGLDSAPAEVSVAM 38
NTTYV + ++SH LD+ + +SV++
Sbjct: 3 NTTYVYIIKIRLSHGTSSLDNIISSLSVSL 92
>TC85449 homologue to PIR|S71923|S71923 cysteine proteinase (EC 3.4.22.-) -
garden pea, complete
Length = 1534
Score = 25.0 bits (53), Expect = 8.4
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +3
Query: 97 CGEVDMEVKMSVMEVGYRLKGGL 119
CG M+V +V+ VGY ++ G+
Sbjct: 930 CGSTPMDVNHAVLAVGYGIEDGV 998
>TC81064 similar to GP|15451210|gb|AAK96876.1 Unknown protein {Arabidopsis
thaliana}, partial (24%)
Length = 928
Score = 25.0 bits (53), Expect = 8.4
Identities = 17/57 (29%), Positives = 28/57 (48%), Gaps = 3/57 (5%)
Frame = +2
Query: 76 EIRVPSKRDDGWMEIELGEFFCGEVDMEVKMSV---MEVGYRLKGGLIVEGIEVRPK 129
+IRV + DD + +ELG F DM ++V +G+ + L + G+ V K
Sbjct: 758 DIRVLRQCDDDHLVLELGMNFLTGDDMSAILAVKLRKRLGFGMSAKLHITGMHVEXK 928
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.136 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,247,679
Number of Sequences: 36976
Number of extensions: 34584
Number of successful extensions: 183
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 175
length of query: 129
length of database: 9,014,727
effective HSP length: 85
effective length of query: 44
effective length of database: 5,871,767
effective search space: 258357748
effective search space used: 258357748
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC147963.2