BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147538.1 + phase: 0
(138 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79948 similar to PIR|A84830|A84830 hypothetical protein At2g40... 34 0.021
TC86628 similar to PIR|T08971|T08971 hypothetical protein F19B15... 28 1.2
TC93545 similar to PIR|F84581|F84581 copia-like retroelement pol... 27 3.4
TC90759 similar to GP|20259313|gb|AAM14392.1 unknown protein {Ar... 26 4.4
TC90690 similar to GP|16974581|gb|AAL31186.1 At1g52150/F5F19_21 ... 26 5.8
TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 25 7.6
CA991311 weakly similar to SP|O81973|C933 Cytochrome P450 93A3 (... 25 7.6
TC83625 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 25 7.6
AW560893 25 7.6
AW685995 similar to PIR|I51618|I516 nucleolar phosphoprotein - A... 25 9.9
TC77577 GP|21615419|emb|CAD33929. microtubule associated protein... 25 9.9
TC78342 similar to GP|8571474|gb|AAF76897.1| proline/glycine bet... 25 9.9
>TC79948 similar to PIR|A84830|A84830 hypothetical protein At2g40480
[imported] - Arabidopsis thaliana, partial (28%)
Length = 1128
Score = 33.9 bits (76), Expect = 0.021
Identities = 28/113 (24%), Positives = 56/113 (48%)
Frame = +3
Query: 21 DSAIEKTKGETADGDKDEIIKELKKVKKQNFVTHCLLSAMIVLTVAWQLSEVSLVMRVKD 80
D I+K + + A+ +KD I+KEL+ + + + + IV + QL + +L +
Sbjct: 234 DFDIKKVEEQAAELEKDLIVKELETL---DVLEELGATKRIVEDLKQQLQKEALKLSATP 404
Query: 81 GMNHPFRSVGNMFKEMVKDVSGSNGQDAAGDKENNESTSLPSLKIPDMTNVDV 133
+N + + KEM K+ +G+N + ++ + S S+ PDM +D+
Sbjct: 405 DLNSNEQVGTPVIKEMNKETNGNNVNN--NQEQTSHIPSSCSMSSPDMILMDL 557
>TC86628 similar to PIR|T08971|T08971 hypothetical protein F19B15.190 -
Arabidopsis thaliana, partial (88%)
Length = 743
Score = 28.1 bits (61), Expect = 1.2
Identities = 12/37 (32%), Positives = 23/37 (61%)
Frame = +3
Query: 11 SEASSPLKQVDSAIEKTKGETADGDKDEIIKELKKVK 47
+E + +KQ+ A+ G AD D+DE+ EL++++
Sbjct: 528 NEQTENMKQIQEALSAPIGAAADFDEDELEAELEELE 638
>TC93545 similar to PIR|F84581|F84581 copia-like retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(72%)
Length = 661
Score = 26.6 bits (57), Expect = 3.4
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = +1
Query: 11 SEASSPLKQVDSAIEKTKGETADGDKDEIIKELKKVKKQNF 51
+E + +KQ+ + G AD D+DE+ EL+ ++ F
Sbjct: 538 NEQTENMKQIQELLSAPIGAAADFDEDELEAELEXLEGAEF 660
>TC90759 similar to GP|20259313|gb|AAM14392.1 unknown protein {Arabidopsis
thaliana}, partial (49%)
Length = 785
Score = 26.2 bits (56), Expect = 4.4
Identities = 15/50 (30%), Positives = 28/50 (56%), Gaps = 7/50 (14%)
Frame = +1
Query: 35 DKDEIIKELKKVKKQNFV-THCLLSAMIVLTVAWQL------SEVSLVMR 77
+KD+++ E KKVK + + HC +S L +A + E++LV++
Sbjct: 364 EKDDVVAEWKKVKNEMCLHVHCFVSGPTFLDLAAEFRYHIFSKEMTLVLK 513
>TC90690 similar to GP|16974581|gb|AAL31186.1 At1g52150/F5F19_21
{Arabidopsis thaliana}, partial (20%)
Length = 552
Score = 25.8 bits (55), Expect = 5.8
Identities = 12/31 (38%), Positives = 19/31 (60%)
Frame = +3
Query: 54 HCLLSAMIVLTVAWQLSEVSLVMRVKDGMNH 84
H L+ + V+ +W LS ++L+MR G NH
Sbjct: 321 HLLVGSATVIGASWVLSYLNLIMR---GANH 404
>TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (13%)
Length = 663
Score = 25.4 bits (54), Expect = 7.6
Identities = 12/32 (37%), Positives = 19/32 (58%)
Frame = +3
Query: 18 KQVDSAIEKTKGETADGDKDEIIKELKKVKKQ 49
K+ + E KGE DGD+ + ++ KK KK+
Sbjct: 261 KKKEKKEENVKGEEEDGDEKKDKEKKKKEKKE 356
>CA991311 weakly similar to SP|O81973|C933 Cytochrome P450 93A3 (EC 1.14.-.-)
(P450 CP5). [Soybean] {Glycine max}, partial (33%)
Length = 768
Score = 25.4 bits (54), Expect = 7.6
Identities = 12/43 (27%), Positives = 20/43 (45%)
Frame = +3
Query: 81 GMNHPFRSVGNMFKEMVKDVSGSNGQDAAGDKENNESTSLPSL 123
G N + + NMF M++ V + ++ KEN E + L
Sbjct: 432 GFNKRLKELMNMFDTMIESVIREHQEEMKKRKENGEGAHVKDL 560
>TC83625 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (9%)
Length = 908
Score = 25.4 bits (54), Expect = 7.6
Identities = 12/32 (37%), Positives = 19/32 (58%)
Frame = +2
Query: 18 KQVDSAIEKTKGETADGDKDEIIKELKKVKKQ 49
K+ + E KGE DGD+ + ++ KK KK+
Sbjct: 653 KKKEKKEENVKGEEEDGDEKKDKEKKKKEKKE 748
>AW560893
Length = 629
Score = 25.4 bits (54), Expect = 7.6
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = -1
Query: 36 KDEIIKELKKVKKQNFVTHCLL 57
K E KKVKK F+ CLL
Sbjct: 179 KSECTSSYKKVKKMRFLIQCLL 114
>AW685995 similar to PIR|I51618|I516 nucleolar phosphoprotein - African
clawed frog, partial (5%)
Length = 516
Score = 25.0 bits (53), Expect = 9.9
Identities = 16/44 (36%), Positives = 29/44 (65%)
Frame = +3
Query: 6 ETVKQSEASSPLKQVDSAIEKTKGETADGDKDEIIKELKKVKKQ 49
+T K+ +A P K+V+ +EKTKG+ A ++E K+ KK +++
Sbjct: 33 KTSKKVKAEEP-KKVEK-VEKTKGKKAAKAEEEKKKQQKKKEEK 158
>TC77577 GP|21615419|emb|CAD33929. microtubule associated protein {Cicer
arietinum}, complete
Length = 992
Score = 25.0 bits (53), Expect = 9.9
Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = -3
Query: 37 DEIIKELKKVKKQNFVTHCLLSAMIVLTVAWQLSEVSLVMRVK-DGMNHPFR 87
++ IKELK ++ F +L + WQL +S+V++ + MN P R
Sbjct: 663 EQSIKELKD*TRKLFNIKHVLRI*FTCSCTWQLRTMSVVIKYHCEFMNKPMR 508
>TC78342 similar to GP|8571474|gb|AAF76897.1| proline/glycine betaine
transporter {Atriplex hortensis}, partial (90%)
Length = 1717
Score = 25.0 bits (53), Expect = 9.9
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +3
Query: 72 VSLVMRVKDGMNHPFR 87
++LV+ +KDGMN P R
Sbjct: 708 IALVLSIKDGMNSPAR 755
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.308 0.127 0.343
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,231,487
Number of Sequences: 36976
Number of extensions: 33190
Number of successful extensions: 163
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of query: 138
length of database: 9,014,727
effective HSP length: 86
effective length of query: 52
effective length of database: 5,834,791
effective search space: 303409132
effective search space used: 303409132
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC147538.1