BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147536.6 + phase: 0
(281 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW560677 similar to GP|21553514|gb| unknown {Arabidopsis thalian... 212 1e-55
BF648773 155 1e-38
AW559952 similar to GP|18700123|gb| At2g39560/F12L6.22 {Arabidop... 118 3e-27
TC91452 weakly similar to GP|21537012|gb|AAM61353.1 unknown {Ara... 38 9e-05
TC90004 37 0.006
TC86952 similar to GP|23498240|emb|CAD49211. hypothetical protei... 30 1.0
TC83218 29 1.8
TC90445 homologue to PIR|C84507|C84507 hypothetical protein At2g... 28 3.0
TC81767 similar to GP|21537034|gb|AAM61375.1 unknown {Arabidopsi... 28 3.0
TC78746 similar to GP|22137234|gb|AAM91462.1 AT5g39360/MUL8_40 {... 28 3.9
TC84913 weakly similar to GP|22136618|gb|AAM91628.1 unknown prot... 28 3.9
AW689094 similar to GP|6016734|gb unknown protein {Arabidopsis t... 28 5.1
BQ751223 similar to GP|16660468|gb chitin synthase {Aspergillus ... 27 6.7
CA860047 weakly similar to GP|15081680|gb| AT3g12390/T2E22_130 {... 27 8.8
>AW560677 similar to GP|21553514|gb| unknown {Arabidopsis thaliana}, partial
(12%)
Length = 534
Score = 212 bits (540), Expect = 1e-55
Identities = 101/105 (96%), Positives = 101/105 (96%)
Frame = -2
Query: 177 TPMNCSPYCSPYNQHGFNPLFESTTDAEFNRLKSSPPPKFKFLQEAEEKLRRKMQDDNKG 236
TPMNCSPYCS YNQHGFNPLFES TDAEFNRLKSSPPPKFKFLQEAEE LRRKMQD NKG
Sbjct: 533 TPMNCSPYCSLYNQHGFNPLFESPTDAEFNRLKSSPPPKFKFLQEAEENLRRKMQDANKG 354
Query: 237 INGNEVDNSLITIIVDKKYEREVNHHQCHLQQYHSSTSQVLPLAS 281
INGNEVDNSLITIIVDKKYEREVNHHQCHLQQYHSSTSQVLPLAS
Sbjct: 353 INGNEVDNSLITIIVDKKYEREVNHHQCHLQQYHSSTSQVLPLAS 219
>BF648773
Length = 542
Score = 155 bits (393), Expect = 1e-38
Identities = 71/72 (98%), Positives = 72/72 (99%)
Frame = +2
Query: 34 KKIIKREIENDYGNGNPLKELFYMFCWKRPSTSLRQTGFTPPELCSSMRINDNFVHDPQV 93
+KIIKREIENDYGNGNPLKELFYMFCWKRPSTSLRQTGFTPPELCSSMRINDNFVHDPQV
Sbjct: 326 QKIIKREIENDYGNGNPLKELFYMFCWKRPSTSLRQTGFTPPELCSSMRINDNFVHDPQV 505
Query: 94 QTSKEFLFKPYG 105
QTSKEFLFKPYG
Sbjct: 506 QTSKEFLFKPYG 541
>AW559952 similar to GP|18700123|gb| At2g39560/F12L6.22 {Arabidopsis
thaliana}, partial (14%)
Length = 604
Score = 118 bits (295), Expect = 3e-27
Identities = 57/58 (98%), Positives = 57/58 (98%)
Frame = +1
Query: 1 MKSLSSVGLGLSIVFGCLLLALVAEVYYLLWWKKKIIKREIENDYGNGNPLKELFYMF 58
MKSLSSV LGLSIVFGCLLLALVAEVYYLLWWKKKIIKREIENDYGNGNPLKELFYMF
Sbjct: 430 MKSLSSVRLGLSIVFGCLLLALVAEVYYLLWWKKKIIKREIENDYGNGNPLKELFYMF 603
>TC91452 weakly similar to GP|21537012|gb|AAM61353.1 unknown {Arabidopsis
thaliana}, partial (10%)
Length = 774
Score = 37.7 bits (86), Expect(2) = 9e-05
Identities = 30/103 (29%), Positives = 47/103 (45%), Gaps = 28/103 (27%)
Frame = +2
Query: 106 EDVIDADYMMQHHHDGLLGHPRFLFTIVEESKEDLESEDGKCGKD-------SRGRSLGD 158
ED ++ +M ++H L G R LFTI EE +E +ESE+G + +GR++
Sbjct: 119 EDELE-QHMAKYHC--LYGPSRVLFTIKEEEREGVESENGSSTTECSSVVTVMKGRAVRS 289
Query: 159 L---------------------LDVETPYLTPIASPHFFTPMN 180
+ LD TPY TP A+ ++TP +
Sbjct: 290 MRLSENVVVGGGDEVVVAVEEWLDESTPYSTPCATSPYYTPQS 418
Score = 25.0 bits (53), Expect(2) = 9e-05
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +1
Query: 69 QTGFTPPELCSSMRINDNFVHD 90
QT TPP CSS + FV D
Sbjct: 40 QTP*TPPSTCSSRTFSSTFVFD 105
>TC90004
Length = 749
Score = 37.4 bits (85), Expect = 0.006
Identities = 14/30 (46%), Positives = 22/30 (72%)
Frame = +2
Query: 6 SVGLGLSIVFGCLLLALVAEVYYLLWWKKK 35
S+ + LS+VFG ++L A ++Y LWWKK+
Sbjct: 644 SLCITLSLVFGFIVLMAFAHIFYFLWWKKR 733
>TC86952 similar to GP|23498240|emb|CAD49211. hypothetical protein
{Plasmodium falciparum 3D7}, partial (0%)
Length = 1103
Score = 30.0 bits (66), Expect = 1.0
Identities = 14/46 (30%), Positives = 21/46 (45%)
Frame = -3
Query: 175 FFTPMNCSPYCSPYNQHGFNPLFESTTDAEFNRLKSSPPPKFKFLQ 220
F + C+P+ P + H F+ F T+ F+ SS F F Q
Sbjct: 612 FHQLLGCAPFSLPRSSHSFSLFFHDTSSTSFSFFSSSHSFSFFFHQ 475
>TC83218
Length = 1170
Score = 29.3 bits (64), Expect = 1.8
Identities = 18/45 (40%), Positives = 20/45 (44%)
Frame = +2
Query: 170 IASPHFFTPMNCSPYCSPYNQHGFNPLFESTTDAEFNRLKSSPPP 214
I PHF NCSP C +H N F T F+ L SP P
Sbjct: 704 IPRPHFTLASNCSPKCCCIPRHVHNTSF---TLYLFSFLSQSPRP 829
>TC90445 homologue to PIR|C84507|C84507 hypothetical protein At2g13370
[imported] - Arabidopsis thaliana, partial (29%)
Length = 1672
Score = 28.5 bits (62), Expect = 3.0
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +1
Query: 226 LRRKMQDDNKGINGNEVDNSLITIIVDKK 254
L R Q+ NKG+ GN+V SL+ I+V+ K
Sbjct: 733 LERNFQNLNKGVRGNQV--SLLNIVVELK 813
>TC81767 similar to GP|21537034|gb|AAM61375.1 unknown {Arabidopsis
thaliana}, partial (90%)
Length = 698
Score = 28.5 bits (62), Expect = 3.0
Identities = 18/58 (31%), Positives = 33/58 (56%), Gaps = 2/58 (3%)
Frame = +2
Query: 34 KKIIKREIENDYGNGNPLKELFYMFCWKRPSTSLRQTGFT--PPELCSSMRINDNFVH 89
+K++++ +E NG+ LK++F WK + R G+T P E SSM ++ F++
Sbjct: 233 EKVVEKNVEM---NGDFLKKMFEKIEWKAFVEASRGMGYTELPEEADSSMLDSNEFLN 397
>TC78746 similar to GP|22137234|gb|AAM91462.1 AT5g39360/MUL8_40
{Arabidopsis thaliana}, partial (97%)
Length = 1222
Score = 28.1 bits (61), Expect = 3.9
Identities = 15/57 (26%), Positives = 25/57 (43%)
Frame = +1
Query: 6 SVGLGLSIVFGCLLLALVAEVYYLLWWKKKIIKREIENDYGNGNPLKELFYMFCWKR 62
S+ L LS+ L + W K + ++ NDY P++ LF++ KR
Sbjct: 28 SLSLSLSLSLSAFLSFKCVSSFLCFSWTKLPLLKDEGNDYHKAVPMRTLFFLPMHKR 198
>TC84913 weakly similar to GP|22136618|gb|AAM91628.1 unknown protein
{Arabidopsis thaliana}, partial (6%)
Length = 930
Score = 28.1 bits (61), Expect = 3.9
Identities = 10/40 (25%), Positives = 22/40 (55%), Gaps = 6/40 (15%)
Frame = -3
Query: 46 GNGNPLKELFYMFCWKRPSTSLRQTGFT------PPELCS 79
G+ + E ++++CW+ P +LR+ +T P +C+
Sbjct: 781 GHSRVIIETYFLYCWRHPLRTLRKVRWTRTANENPSSICN 662
>AW689094 similar to GP|6016734|gb unknown protein {Arabidopsis thaliana},
partial (8%)
Length = 368
Score = 27.7 bits (60), Expect = 5.1
Identities = 12/31 (38%), Positives = 18/31 (57%)
Frame = -2
Query: 49 NPLKELFYMFCWKRPSTSLRQTGFTPPELCS 79
NP KELFY + K + +GF+P ++ S
Sbjct: 304 NPHKELFYKYKLKHACDQILLSGFSPSKVAS 212
>BQ751223 similar to GP|16660468|gb chitin synthase {Aspergillus oryzae},
partial (33%)
Length = 736
Score = 27.3 bits (59), Expect = 6.7
Identities = 40/164 (24%), Positives = 66/164 (39%), Gaps = 3/164 (1%)
Frame = -2
Query: 119 HDG-LLGHPRFLFTIVEESKEDLESEDGKCGKDSRGRSLGDLLDVETPYLTPIASPHFFT 177
HDG +LGH R T++ ESKE L + G D + + + + +L + P
Sbjct: 645 HDGEILGHKRGQVTVLAESKEVLLVK----GVDVANTVVTNDIVGDDEWLALVYGPQ--- 487
Query: 178 PMNCSPYCSPYN--QHGFNPLFESTTDAEFNRLKSSPPPKFKFLQEAEEKLRRKMQDDNK 235
++C N + GF L + D F L PP +F+ E N
Sbjct: 486 AIHCETAWQTGNTAEEGFEGLGQVVGDEVFVYLDHGPP---RFVVVLESSF--TTDTGNL 322
Query: 236 GINGNEVDNSLITIIVDKKYEREVNHHQCHLQQYHSSTSQVLPL 279
G+ G S+ ++ ++ ++HH+ L Q T V+ L
Sbjct: 321 GVVGTGRHKSVERVLAHRRI--GIDHHEV-LVQLGIDTDDVIDL 199
>CA860047 weakly similar to GP|15081680|gb| AT3g12390/T2E22_130 {Arabidopsis
thaliana}, partial (37%)
Length = 543
Score = 26.9 bits (58), Expect = 8.8
Identities = 9/27 (33%), Positives = 18/27 (66%)
Frame = -2
Query: 90 DPQVQTSKEFLFKPYGEDVIDADYMMQ 116
D +++ +KE +F+P D ++ YM+Q
Sbjct: 452 DIRIRNNKENIFRPSDSDTVNTGYMLQ 372
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,961,113
Number of Sequences: 36976
Number of extensions: 171059
Number of successful extensions: 1108
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1107
length of query: 281
length of database: 9,014,727
effective HSP length: 95
effective length of query: 186
effective length of database: 5,502,007
effective search space: 1023373302
effective search space used: 1023373302
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147536.6