BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147497.9 - phase: 0
(148 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87239 similar to PIR|T09028|T09028 hypothetical protein T27E11... 169 3e-43
BQ752218 similar to GP|19170914|emb hypothetical protein {Enceph... 31 0.16
TC80915 similar to PIR|S26025|S26025 NADH dehydrogenase (ubiquin... 30 0.36
BI310469 similar to GP|17979305|gb unknown protein {Arabidopsis ... 30 0.36
TC76514 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 29 0.80
TC89233 homologue to GP|18734|emb|CAA36734.1|| DNA-directed RNA ... 29 0.80
TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 29 0.80
TC76583 similar to GP|8886934|gb|AAF80620.1| F2D10.25 {Arabidops... 29 0.80
TC93050 similar to GP|8886934|gb|AAF80620.1| F2D10.25 {Arabidops... 28 1.4
TC83458 homologue to PIR|T07796|T07796 DNA-directed RNA polymera... 28 1.8
TC78826 similar to PIR|G96607|G96607 probable galactinol synthas... 28 1.8
TC81336 homologue to GP|20198031|gb|AAM15362.1 hypothetical prot... 28 1.8
TC78648 similar to PIR|S51529|S51529 SPF1 protein - sweet potato... 28 1.8
TC82216 similar to GP|16930419|gb|AAL31895.1 AT4g21800/F17L22_26... 27 2.3
AW696403 similar to GP|13346157|gb| bZIP protein DPBF4 {Arabidop... 27 2.3
BQ751141 similar to SP|P29551|EF3 Elongation factor 3 (EF-3). {P... 27 2.3
TC84772 weakly similar to GP|14532846|gb|AAK64105.1 unknown prot... 27 3.0
BG451281 similar to PIR|T06063|T060 hypothetical protein F19H22.... 27 3.0
BF649374 homologue to GP|11762186|gb| AT4g27960 {Arabidopsis tha... 27 3.0
TC80296 similar to PIR|H86265|H86265 protein F3F19.18 [imported]... 27 3.0
>TC87239 similar to PIR|T09028|T09028 hypothetical protein T27E11.130 -
Arabidopsis thaliana, partial (69%)
Length = 1228
Score = 169 bits (429), Expect = 3e-43
Identities = 96/125 (76%), Positives = 102/125 (80%), Gaps = 10/125 (8%)
Frame = +1
Query: 28 KTQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAI 87
+TQTSS S QPK SKPIPFSSTFDPSNPTAFL+NVFDFIAKEST FFDNDSAEKVVLSA+
Sbjct: 88 ETQTSSSS-QPKPSKPIPFSSTFDPSNPTAFLENVFDFIAKESTDFFDNDSAEKVVLSAV 264
Query: 88 CTVKVK--------KAKITVEEKMKVDKAAAV--AEKKAKDVYEKEDEKKDEESGLTAPN 137
VKVK KAKI EEK KVDKAA+V AEKKAK+V EKED+KKD ESGL APN
Sbjct: 265 RAVKVKKAKAVAAEKAKIAAEEKAKVDKAASVAAAEKKAKEVDEKEDDKKDGESGLAAPN 444
Query: 138 QGNLM 142
QGN M
Sbjct: 445 QGNGM 459
>BQ752218 similar to GP|19170914|emb hypothetical protein {Encephalitozoon
cuniculi}, partial (5%)
Length = 637
Score = 31.2 bits (69), Expect = 0.16
Identities = 20/44 (45%), Positives = 28/44 (63%)
Frame = -2
Query: 91 KVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGLT 134
K K+ K E+K+K +K A EKKAK +KE ++KD ES L+
Sbjct: 483 KAKEEKRQAEKKIKEEKKEA--EKKAKKD-KKEQDRKDAESALS 361
>TC80915 similar to PIR|S26025|S26025 NADH dehydrogenase (ubiquinone) (EC
1.6.5.3) chain 5 - pig roundworm mitochondrion, partial
(5%)
Length = 799
Score = 30.0 bits (66), Expect = 0.36
Identities = 18/66 (27%), Positives = 36/66 (54%), Gaps = 3/66 (4%)
Frame = -3
Query: 40 SSKPIPFSSTFDPSNPTAFLKNVFDFIAKEST--SFFD-NDSAEKVVLSAICTVKVKKAK 96
SSKP S +P+NP+++ F++++K +T F + S + +L I +K + +
Sbjct: 698 SSKPPSISFISNPTNPSSYFTLDFNWVSKLTTFCGFLNLGVSENRRILKQIPKIKTRPMR 519
Query: 97 ITVEEK 102
+ + EK
Sbjct: 518 LKIREK 501
>BI310469 similar to GP|17979305|gb unknown protein {Arabidopsis thaliana},
partial (81%)
Length = 793
Score = 30.0 bits (66), Expect = 0.36
Identities = 20/61 (32%), Positives = 32/61 (51%)
Frame = +3
Query: 10 IKKSFNVFVTKRIIVQTVKTQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKE 69
I KSF + K + + +K + P+ +PK +K +PF ST TAFL F +A +
Sbjct: 168 IVKSFPIRKAKPL--ELLKVLSFPPTTKPKDNKKLPFPST------TAFLPTNFTILAHQ 323
Query: 70 S 70
+
Sbjct: 324 A 326
>TC76514 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (61%)
Length = 1288
Score = 28.9 bits (63), Expect = 0.80
Identities = 14/32 (43%), Positives = 18/32 (55%)
Frame = +3
Query: 100 EEKMKVDKAAAVAEKKAKDVYEKEDEKKDEES 131
E+ V K AAVA+K KD + +DE D S
Sbjct: 882 EDAKPVSKPAAVAKKSKKDSSDSDDEDDDSSS 977
>TC89233 homologue to GP|18734|emb|CAA36734.1|| DNA-directed RNA polymerase
{Glycine max}, partial (21%)
Length = 648
Score = 28.9 bits (63), Expect = 0.80
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
+SPSY P S P S ++ PS+PT
Sbjct: 84 TSPSYSPTSPSYSPTSPSYSPSSPT 158
Score = 28.5 bits (62), Expect = 1.0
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +3
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNP 55
+SPSY P S P S T+ PS+P
Sbjct: 105 TSPSYSPTSPSYSPSSPTYSPSSP 176
>TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (69%)
Length = 1615
Score = 28.9 bits (63), Expect = 0.80
Identities = 14/32 (43%), Positives = 18/32 (55%)
Frame = +3
Query: 100 EEKMKVDKAAAVAEKKAKDVYEKEDEKKDEES 131
E+ V K AAVA+K KD + +DE D S
Sbjct: 204 EDAKPVSKPAAVAKKSKKDSSDSDDEDDDSSS 299
>TC76583 similar to GP|8886934|gb|AAF80620.1| F2D10.25 {Arabidopsis
thaliana}, partial (3%)
Length = 794
Score = 28.9 bits (63), Expect = 0.80
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +3
Query: 30 QTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDS 78
+TS S+ FSS+ P P +L+N FDF +S S D+ S
Sbjct: 78 KTSGFSFDDSVPSTPLFSSSSSPQRPKDWLENAFDFSRFDSFSTHDSVS 224
>TC93050 similar to GP|8886934|gb|AAF80620.1| F2D10.25 {Arabidopsis
thaliana}, partial (4%)
Length = 800
Score = 28.1 bits (61), Expect = 1.4
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +3
Query: 46 FSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDS 78
FSS+ P P +L+N FDF +S S D+ S
Sbjct: 678 FSSSSSPQRPKNWLENTFDFSRFDSFSTHDSVS 776
>TC83458 homologue to PIR|T07796|T07796 DNA-directed RNA polymerase (EC
2.7.7.6) largest chain - soybean (fragment), partial
(63%)
Length = 1220
Score = 27.7 bits (60), Expect = 1.8
Identities = 11/24 (45%), Positives = 16/24 (65%)
Frame = +2
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNP 55
+SPSY P S P SS++ P++P
Sbjct: 1049 TSPSYSPTSPSYSPTSSSYSPTSP 1120
Score = 26.2 bits (56), Expect = 5.2
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +2
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
+SPSY P S P S ++ P++P+
Sbjct: 1007 TSPSYSPTSPSYSPTSPSYSPTSPS 1081
Score = 25.8 bits (55), Expect = 6.8
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +2
Query: 31 TSSPSYQPKSSKPIPFSSTFDPSNP 55
TSSP Y P S P S + P++P
Sbjct: 815 TSSPGYSPSSPGYSPSSPGYSPTSP 889
Score = 25.4 bits (54), Expect = 8.8
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
SSP Y P S P S ++ P++P+
Sbjct: 965 SSPGYSPTSPAYSPTSPSYSPTSPS 1039
>TC78826 similar to PIR|G96607|G96607 probable galactinol synthase F25P12.95
[imported] - Arabidopsis thaliana, partial (83%)
Length = 1238
Score = 27.7 bits (60), Expect = 1.8
Identities = 24/92 (26%), Positives = 38/92 (41%), Gaps = 7/92 (7%)
Frame = +3
Query: 39 KSSKPIPFSSTF-------DPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVK 91
K +KP PF+ D P + NV++ + +N EKV + C
Sbjct: 636 KITKPTPFAEQDFLNMYFKDKYKP---IPNVYNLVLAMMWRHPENVELEKVQVVHYCAAG 806
Query: 92 VKKAKITVEEKMKVDKAAAVAEKKAKDVYEKE 123
K + T EE+ + + KK K++YE E
Sbjct: 807 SKPWRYTGEEQNMDREDIKMLVKKWKEIYEDE 902
>TC81336 homologue to GP|20198031|gb|AAM15362.1 hypothetical protein
{Arabidopsis thaliana}, partial (2%)
Length = 1542
Score = 27.7 bits (60), Expect = 1.8
Identities = 14/62 (22%), Positives = 30/62 (47%)
Frame = +1
Query: 69 ESTSFFDNDSAEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKD 128
EST+ D + + +S++C+ + + EK + + K+ DV ++E+ +D
Sbjct: 1033 ESTAKMQADVGDGMDISSVCSSAAVSELVELSEKDSIGNSETDVTKEKDDVAQEENAPRD 1212
Query: 129 EE 130
E
Sbjct: 1213 *E 1218
>TC78648 similar to PIR|S51529|S51529 SPF1 protein - sweet potato, partial
(18%)
Length = 990
Score = 27.7 bits (60), Expect = 1.8
Identities = 20/67 (29%), Positives = 29/67 (42%), Gaps = 13/67 (19%)
Frame = +1
Query: 28 KTQTSSPSYQPKSSKP---IPFSSTFDP----------SNPTAFLKNVFDFIAKESTSFF 74
K ++ P P SS P +PFSSTF P S+P F + A +S ++
Sbjct: 235 KFKSVQPPSLPLSSSPSFHLPFSSTFSPTDFLISPFFLSSPNVFASPTTEAFANQSFNWN 414
Query: 75 DNDSAEK 81
N E+
Sbjct: 415 KNSLGEE 435
>TC82216 similar to GP|16930419|gb|AAL31895.1 AT4g21800/F17L22_260
{Arabidopsis thaliana}, partial (31%)
Length = 702
Score = 27.3 bits (59), Expect = 2.3
Identities = 25/100 (25%), Positives = 45/100 (45%), Gaps = 7/100 (7%)
Frame = -1
Query: 5 TATSSIKKSFNVFVTKRIIVQTVKTQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLK-NVF 63
+++SSI S +V+V K + + + +SS S KP+ ++ P + L ++F
Sbjct: 453 SSSSSIASSSSVYVPKSTLSSSSISSSSSSSSSSLFFKPVLNTTVSPPDFSISLLSLSMF 274
Query: 64 DFIAKESTSFFDNDSAEKVVL------SAICTVKVKKAKI 97
F S S F + + L SA+ + +KK I
Sbjct: 273 SFRRFSSNSCFSSRLLSRSAL*VSMYSSALASTALKKPSI 154
>AW696403 similar to GP|13346157|gb| bZIP protein DPBF4 {Arabidopsis
thaliana}, partial (28%)
Length = 587
Score = 27.3 bits (59), Expect = 2.3
Identities = 21/95 (22%), Positives = 40/95 (42%)
Frame = +2
Query: 54 NPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAE 113
N LKNV+ +ST+ + +A+ ++ ++ + + AA ++
Sbjct: 338 NLDELLKNVWTVEVNQSTNTDNEGTAQS-------------SEACLQRQASLALKAAFSK 478
Query: 114 KKAKDVYEKEDEKKDEESGLTAPNQGNLMRVIIGG 148
K +V+ +KKD E + Q L + IGG
Sbjct: 479 KTVDEVWRDIQQKKDSEEKKSRERQTTLGEIDIGG 583
>BQ751141 similar to SP|P29551|EF3 Elongation factor 3 (EF-3). {Pneumocystis
carinii}, partial (15%)
Length = 569
Score = 27.3 bits (59), Expect = 2.3
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = -2
Query: 93 KKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDE 125
KKAK+T E K K K+ ++V+ ED+
Sbjct: 154 KKAKLTSSEARKKKKERMARRKRGEEVFSDEDD 56
>TC84772 weakly similar to GP|14532846|gb|AAK64105.1 unknown protein
{Arabidopsis thaliana}, partial (17%)
Length = 683
Score = 26.9 bits (58), Expect = 3.0
Identities = 15/36 (41%), Positives = 20/36 (54%), Gaps = 2/36 (5%)
Frame = +2
Query: 31 TSSPSYQPKSSKPIPFSS--TFDPSNPTAFLKNVFD 64
T SPS PK + P+P +S + P +PT VFD
Sbjct: 200 TPSPSLTPKPATPLPSASSKSMFPPSPTLCSTLVFD 307
>BG451281 similar to PIR|T06063|T060 hypothetical protein F19H22.130 -
Arabidopsis thaliana, partial (12%)
Length = 556
Score = 26.9 bits (58), Expect = 3.0
Identities = 15/42 (35%), Positives = 21/42 (49%), Gaps = 3/42 (7%)
Frame = +3
Query: 24 VQTVKTQTSSPSYQPK---SSKPIPFSSTFDPSNPTAFLKNV 62
+ T + SSP P S P S+ F PS P+ FLK++
Sbjct: 93 IDTSSSSLSSPLSSPPKP*SQITFPLSNHFSPSPPSPFLKSL 218
>BF649374 homologue to GP|11762186|gb| AT4g27960 {Arabidopsis thaliana},
partial (14%)
Length = 418
Score = 26.9 bits (58), Expect = 3.0
Identities = 15/68 (22%), Positives = 33/68 (48%)
Frame = -1
Query: 7 TSSIKKSFNVFVTKRIIVQTVKTQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFI 66
+ S +K F + + +++T+K Q ++ KS K ++S + ++ N+F ++
Sbjct: 295 SKSPQKPFK*QIHNKSLIKTIKQQKGIQIFKNKSLKSYYYASNYKS*KSKLYVLNIF-YM 119
Query: 67 AKESTSFF 74
KE F
Sbjct: 118 IKEKKGLF 95
>TC80296 similar to PIR|H86265|H86265 protein F3F19.18 [imported] -
Arabidopsis thaliana, partial (34%)
Length = 1734
Score = 26.9 bits (58), Expect = 3.0
Identities = 26/90 (28%), Positives = 39/90 (42%), Gaps = 3/90 (3%)
Frame = -3
Query: 12 KSFNV-FVTK--RIIVQTVKTQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAK 68
KSFNV FV+ RI + + P SS+ P SST S+P + F +
Sbjct: 1096 KSFNVVFVSTLPRICRSPSPSCSEIPPSSSLSSRSSPSSSTLSASSPLVS*SELLSFSSS 917
Query: 69 ESTSFFDNDSAEKVVLSAICTVKVKKAKIT 98
++ S S+ VLS + + K+T
Sbjct: 916 DTPS*SSASSSSLPVLSLPSWLSSSEFKLT 827
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.126 0.336
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,427,573
Number of Sequences: 36976
Number of extensions: 37895
Number of successful extensions: 379
Number of sequences better than 10.0: 66
Number of HSP's better than 10.0 without gapping: 361
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 375
length of query: 148
length of database: 9,014,727
effective HSP length: 87
effective length of query: 61
effective length of database: 5,797,815
effective search space: 353666715
effective search space used: 353666715
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC147497.9