BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147496.8 + phase: 1 /partial
(120 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF650795 similar to GP|20161490|dbj beta-1 3 glucanase-like prot... 185 4e-48
TC78740 similar to GP|3482921|gb|AAC33206.1| Unknown protein {Ar... 141 7e-35
BG585334 similar to GP|20161490|db beta-1 3 glucanase-like prote... 124 1e-29
TC90032 similar to GP|14090345|dbj|BAB55504. contains EST D15166... 102 3e-23
CA920150 similar to GP|18700155|gb| AT5g55180/MCO15_13 {Arabidop... 100 1e-22
TC89701 similar to PIR|T06662|T06662 hypothetical protein T6G15.... 100 2e-22
TC79599 similar to GP|9757867|dbj|BAB08454.1 gb|AAD30228.1~gene_... 96 4e-21
TC90592 similar to GP|21618307|gb|AAM67357.1 unknown {Arabidopsi... 93 3e-20
TC86647 similar to PIR|E84471|E84471 probable beta-1 3-glucanase... 92 4e-20
TC84652 similar to GP|14279169|gb|AAK58515.1 beta-1 3-glucanase-... 91 8e-20
TC81764 similar to GP|17104805|gb|AAL34291.1 putative glucan end... 84 1e-17
TC92433 weakly similar to GP|21592859|gb|AAM64809.1 unknown {Ara... 82 6e-17
TC79296 weakly similar to GP|14279169|gb|AAK58515.1 beta-1 3-glu... 80 1e-16
TC86615 weakly similar to GP|8778409|gb|AAF79417.1| F16A14.5 {Ar... 80 2e-16
AA660501 similar to GP|21537028|gb| unknown {Arabidopsis thalian... 77 2e-15
TC87810 similar to GP|21592752|gb|AAM64701.1 unknown {Arabidopsi... 76 3e-15
BG587358 similar to GP|6041834|gb|A putative glucan endo-1-3-bet... 75 8e-15
AL370014 similar to GP|14279169|gb| beta-1 3-glucanase-like prot... 75 8e-15
TC87078 similar to PIR|T50645|T50645 glucan endo-1 3-beta-D-gluc... 73 2e-14
TC80310 weakly similar to GP|20160496|dbj|BAB89448. beta-1 3-glu... 68 7e-13
>BF650795 similar to GP|20161490|dbj beta-1 3 glucanase-like protein {Oryza
sativa (japonica cultivar-group)}, partial (30%)
Length = 668
Score = 185 bits (469), Expect = 4e-48
Identities = 85/110 (77%), Positives = 96/110 (87%), Gaps = 1/110 (0%)
Frame = +3
Query: 1 TYSPSSTVPITAPSTSNS-PVSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPG 59
T SP+ST+P T P+T NS P+SSG+SWC+ASPSASQ LQVALDYACGYGGTDCSAIQPG
Sbjct: 186 TVSPTSTLPYTNPTTVNSFPISSGSSWCVASPSASQIGLQVALDYACGYGGTDCSAIQPG 365
Query: 60 GSCYNPNSVHDHASFAFNKYYQKNPVPNSCNFGGNAVLTNTNPSKASTIY 109
GSCYNPNS+HD AS+AFNKYY KNPVPNSCNFGG AV+T+TNPS + Y
Sbjct: 366 GSCYNPNSIHDLASYAFNKYYHKNPVPNSCNFGGTAVITSTNPSTGTCQY 515
>TC78740 similar to GP|3482921|gb|AAC33206.1| Unknown protein {Arabidopsis
thaliana}, partial (45%)
Length = 1624
Score = 141 bits (355), Expect = 7e-35
Identities = 64/107 (59%), Positives = 77/107 (71%), Gaps = 2/107 (1%)
Frame = +2
Query: 5 SSTVPIT--APSTSNSPVSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSC 62
+ TVP+T PS+++ P SSG SWC+A P Q +LQ ALDYACG GTDCS I GG C
Sbjct: 527 TGTVPVTNPPPSSTSPPSSSGGSWCVAKPGTPQSTLQTALDYACGTKGTDCSQINQGGIC 706
Query: 63 YNPNSVHDHASFAFNKYYQKNPVPNSCNFGGNAVLTNTNPSKASTIY 109
YNPNS+ +HASFAFN YY KNP SC+FGG A +TNTNPS + I+
Sbjct: 707 YNPNSLQNHASFAFNSYYVKNPAATSCDFGGVATITNTNPSSGTCIF 847
>BG585334 similar to GP|20161490|db beta-1 3 glucanase-like protein {Oryza
sativa (japonica cultivar-group)}, partial (39%)
Length = 835
Score = 124 bits (310), Expect = 1e-29
Identities = 60/105 (57%), Positives = 74/105 (70%), Gaps = 2/105 (1%)
Frame = +1
Query: 1 TYSPSSTVPITAPSTSNSPVSSGAS--WCIASPSASQRSLQVALDYACGYGGTDCSAIQP 58
T S TV T P T +S G WC+AS SA++ +L+VALDYACGYG DCS +Q
Sbjct: 406 TTPTSPTVTPTTPGTGSSGGGGGGGGQWCVASESAAETTLKVALDYACGYGA-DCSQLQQ 582
Query: 59 GGSCYNPNSVHDHASFAFNKYYQKNPVPNSCNFGGNAVLTNTNPS 103
GG+CY+PN++ DHAS+AFN YYQKNP P SC FGG A LT+ +PS
Sbjct: 583 GGACYDPNTLKDHASYAFNDYYQKNPAPTSCVFGGVASLTSKDPS 717
>TC90032 similar to GP|14090345|dbj|BAB55504. contains EST
D15166(C0193)~unknown protein {Oryza sativa (japonica
cultivar-group)}, partial (27%)
Length = 1286
Score = 102 bits (254), Expect = 3e-23
Identities = 48/104 (46%), Positives = 71/104 (68%), Gaps = 2/104 (1%)
Frame = +2
Query: 8 VPITAPSTSNSPVSSGASWCIASPSASQRSLQVALDYACGYGGT-DCSAIQPGGSCYNPN 66
V +TA + + SP S+G WCIAS +A+Q LQ A+++ACG G DC+AIQP C+ P+
Sbjct: 437 VDMTADANATSPTSNGTKWCIASSNATQLDLQNAINWACGTSGNVDCTAIQPSQPCFEPD 616
Query: 67 SVHDHASFAFNKYYQKNPVPN-SCNFGGNAVLTNTNPSKASTIY 109
++ HAS+AFN YYQ+N + +C+FGG VL + +P+ + IY
Sbjct: 617 NLVSHASYAFNSYYQQNGASDVACSFGGTGVLVDKDPTYDNCIY 748
>CA920150 similar to GP|18700155|gb| AT5g55180/MCO15_13 {Arabidopsis
thaliana}, partial (19%)
Length = 642
Score = 100 bits (250), Expect = 1e-22
Identities = 50/119 (42%), Positives = 67/119 (56%), Gaps = 10/119 (8%)
Frame = -1
Query: 5 SSTVPITAPSTSNSPVSSG---------ASWCIASPSASQRSLQVALDYACGYGGTDCSA 55
S+ P+ S PVS G +WC+A+ S+ L+ ALDYACG GG DC+
Sbjct: 588 SAQAPVAGVGRSKVPVSGGDVSVVENKGQTWCVANGGYSEEKLKHALDYACGEGGADCAP 409
Query: 56 IQPGGSCYNPNSVHDHASFAFNKYYQ-KNPVPNSCNFGGNAVLTNTNPSKASTIYDHGF 113
IQPG +CYNPN++ HASFAFN YYQ K+ +C+FGG A + P + G+
Sbjct: 408 IQPGATCYNPNTLEAHASFAFNSYYQKKSRGGGTCDFGGAAYVVTQAPRYGKCDFPTGY 232
>TC89701 similar to PIR|T06662|T06662 hypothetical protein T6G15.150 -
Arabidopsis thaliana, partial (31%)
Length = 948
Score = 100 bits (248), Expect = 2e-22
Identities = 50/114 (43%), Positives = 66/114 (57%), Gaps = 3/114 (2%)
Frame = +2
Query: 4 PSSTVPITAPSTSNSPVSSG--ASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGS 61
P S + +T T + G ASWC+ AS +LQ ALDYACG G DC +QP G
Sbjct: 98 PVSVILLTITMTMMNVNGQGGSASWCVVRSDASFNALQTALDYACG-AGADCLPLQPDGL 274
Query: 62 CYNPNSVHDHASFAFNKYYQKNP-VPNSCNFGGNAVLTNTNPSKASTIYDHGFK 114
C+ PN++ HAS+AFN YYQK P SC+F G + + T+PS S +Y +K
Sbjct: 275 CFLPNTIQAHASYAFNSYYQKRARAPGSCDFSGTSTIAQTDPSYGSCVYPSYYK 436
>TC79599 similar to GP|9757867|dbj|BAB08454.1
gb|AAD30228.1~gene_id:K9I9.2~similar to unknown protein
{Arabidopsis thaliana}, partial (23%)
Length = 1530
Score = 95.5 bits (236), Expect = 4e-21
Identities = 48/107 (44%), Positives = 68/107 (62%), Gaps = 4/107 (3%)
Frame = +1
Query: 1 TYSPSSTVPITAPSTSNSPVSSGAS--WCIASPSASQRSLQVALDYACGYGGTDCSAI-Q 57
T P + + +PS S P + WC+A PS +LQ ALDYACG GG DC I
Sbjct: 910 TCPPCNPIENGSPSASPYPQIAPVQKLWCVAKPSVPDATLQEALDYACGEGGADCLEITT 1089
Query: 58 PGGSCYNPNSVHDHASFAFNKYYQKNP-VPNSCNFGGNAVLTNTNPS 103
P G+CYNP+++ HAS+AFN Y+QK+ + +C+FGG A+L +++PS
Sbjct: 1090 PQGNCYNPDTLVAHASYAFNSYWQKHKRIGGTCDFGGTAMLIHSDPS 1230
>TC90592 similar to GP|21618307|gb|AAM67357.1 unknown {Arabidopsis
thaliana}, partial (36%)
Length = 683
Score = 92.8 bits (229), Expect = 3e-20
Identities = 42/85 (49%), Positives = 58/85 (67%), Gaps = 1/85 (1%)
Frame = +1
Query: 26 WCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQKNPV 85
WC+A +A +LQ ALD+ACG GG DC IQ GG CY+ NSV + AS+AFN Y+ K+ +
Sbjct: 136 WCVAKNNAEDAALQTALDWACGAGGADCGPIQNGGPCYDVNSVQNTASYAFNDYFLKHGL 315
Query: 86 -PNSCNFGGNAVLTNTNPSKASTIY 109
+SC+F NA +T+ NPS+ +Y
Sbjct: 316 TDDSCSFNNNAAVTSLNPSEFVFVY 390
>TC86647 similar to PIR|E84471|E84471 probable beta-1 3-glucanase [imported]
- Arabidopsis thaliana, partial (33%)
Length = 1087
Score = 92.4 bits (228), Expect = 4e-20
Identities = 42/97 (43%), Positives = 58/97 (59%), Gaps = 1/97 (1%)
Frame = +3
Query: 18 SPVSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFN 77
S ++G +WC+A+P A + LQ ALD+ACG GG DC IQ +C++PN++ HASFAFN
Sbjct: 336 SKSTTGNTWCVANPYADKNKLQDALDFACGEGGADCRPIQNNATCFDPNTLVAHASFAFN 515
Query: 78 KYYQKNP-VPNSCNFGGNAVLTNTNPSKASTIYDHGF 113
YYQK SC FGG + + P + G+
Sbjct: 516 SYYQKQARAGGSCYFGGTSYVVTQEPKYGKCEFPTGY 626
>TC84652 similar to GP|14279169|gb|AAK58515.1 beta-1 3-glucanase-like
protein {Olea europaea}, partial (24%)
Length = 788
Score = 91.3 bits (225), Expect = 8e-20
Identities = 49/110 (44%), Positives = 60/110 (54%), Gaps = 2/110 (1%)
Frame = +2
Query: 5 SSTVPIT-APSTSNSPVSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCY 63
S P+T AP T++ WCI S LQ LDYAC G DC IQPGG+CY
Sbjct: 170 SPNAPVTPAPKTTSQ-------WCIPKGGVSDDQLQANLDYACSREGMDCGPIQPGGACY 328
Query: 64 NPNSVHDHASFAFNKYYQK-NPVPNSCNFGGNAVLTNTNPSKASTIYDHG 112
+PN+V H++FA N YYQK P +C+F A LT+ NPS Y G
Sbjct: 329 DPNTVASHSAFAMNLYYQKFGRNPWNCDFSQTASLTSQNPSYNGCTYPSG 478
>TC81764 similar to GP|17104805|gb|AAL34291.1 putative glucan endo-1
3-beta-glucosidase precursor {Arabidopsis thaliana},
partial (71%)
Length = 1560
Score = 84.3 bits (207), Expect = 1e-17
Identities = 42/94 (44%), Positives = 58/94 (61%), Gaps = 3/94 (3%)
Frame = +1
Query: 16 SNSPVSSGAS--WCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHAS 73
+ SP S ++ +C+A A LQ L +ACG GG +C+ IQ G CY PN+V HAS
Sbjct: 901 ARSPGSGNSTGIFCVAKDGADTDKLQNGLSWACGQGGANCAPIQQGQRCYLPNNVKSHAS 1080
Query: 74 FAFNKYYQKNP-VPNSCNFGGNAVLTNTNPSKAS 106
+A+N YYQKN V +C+F G A +T+ +PS S
Sbjct: 1081YAYNDYYQKNQGVGGTCDFDGTAEITSKDPSYGS 1182
>TC92433 weakly similar to GP|21592859|gb|AAM64809.1 unknown {Arabidopsis
thaliana}, partial (53%)
Length = 686
Score = 81.6 bits (200), Expect = 6e-17
Identities = 41/86 (47%), Positives = 51/86 (58%), Gaps = 1/86 (1%)
Frame = +3
Query: 22 SGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQ 81
S A +C+ S + LQ A+DYACG G DCS I G C+ PN+V DH ++A N YYQ
Sbjct: 165 SSALYCVCKDGVSSQLLQKAIDYACGTGA-DCSPILQNGPCFQPNTVKDHCNYAVNSYYQ 341
Query: 82 -KNPVPNSCNFGGNAVLTNTNPSKAS 106
K V SC+F G A T T P+ AS
Sbjct: 342 RKGNVQGSCDFAGAAAPTQTPPTAAS 419
>TC79296 weakly similar to GP|14279169|gb|AAK58515.1 beta-1 3-glucanase-like
protein {Olea europaea}, partial (90%)
Length = 1710
Score = 80.5 bits (197), Expect = 1e-16
Identities = 48/120 (40%), Positives = 63/120 (52%), Gaps = 2/120 (1%)
Frame = +3
Query: 3 SPSSTVPIT-APSTSNSPVSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGS 61
+P ST P+T AP TS A WC+ S LQ +DYAC G DC I PGG+
Sbjct: 1158 NPPSTSPVTPAPITS-------AGWCVPKAGVSDAQLQNNIDYACSQG-IDCGPILPGGA 1313
Query: 62 CYNPNSVHDHASFAFNKYYQK-NPVPNSCNFGGNAVLTNTNPSKASTIYDHGFKLQSDTT 120
C+ PN+V HA+F N YYQ +C+F A LT+ NPS + Y G ++D +
Sbjct: 1314 CFEPNTVASHAAFVMNLYYQTFGRNQWNCDFTQTAKLTSQNPSYNACNYAGGST*KADAS 1493
>TC86615 weakly similar to GP|8778409|gb|AAF79417.1| F16A14.5 {Arabidopsis
thaliana}, partial (31%)
Length = 1278
Score = 80.1 bits (196), Expect = 2e-16
Identities = 35/88 (39%), Positives = 54/88 (60%), Gaps = 1/88 (1%)
Frame = +2
Query: 21 SSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYY 80
SSGA +C+ ++LQ A+DYACG G DC+ IQ G C+ PN++ DH ++A N Y+
Sbjct: 245 SSGALYCVCKDGVGDQNLQKAIDYACG-AGADCTQIQQNGPCFQPNTIKDHCNYAVNSYF 421
Query: 81 Q-KNPVPNSCNFGGNAVLTNTNPSKAST 107
Q K +C+F G A + T P+ +++
Sbjct: 422 QKKGQAQGACDFAGMATPSQTPPTSSTS 505
>AA660501 similar to GP|21537028|gb| unknown {Arabidopsis thaliana}, partial
(65%)
Length = 581
Score = 76.6 bits (187), Expect = 2e-15
Identities = 39/74 (52%), Positives = 47/74 (62%), Gaps = 1/74 (1%)
Frame = +2
Query: 38 LQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQK-NPVPNSCNFGGNAV 96
LQ AL++ACG GG DCS IQ CY PN++ DHAS+AFN Y+QK SC F G A+
Sbjct: 20 LQDALNWACGKGGADCSKIQQDQPCYFPNTLKDHASYAFNSYFQKFKNNGGSCYFRGAAM 199
Query: 97 LTNTNPSKASTIYD 110
T +PS S YD
Sbjct: 200 TTEVDPSHDSCHYD 241
>TC87810 similar to GP|21592752|gb|AAM64701.1 unknown {Arabidopsis
thaliana}, partial (52%)
Length = 1094
Score = 76.3 bits (186), Expect = 3e-15
Identities = 37/91 (40%), Positives = 50/91 (54%), Gaps = 1/91 (1%)
Frame = +3
Query: 20 VSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKY 79
+ S A+WC+ A LQ LDYACG G DC+ + CYNPN+V H S+A N Y
Sbjct: 210 IPSSANWCVCKDGADA-ILQKTLDYACG-AGADCNPLHTNAPCYNPNTVRAHCSYAVNSY 383
Query: 80 YQ-KNPVPNSCNFGGNAVLTNTNPSKASTIY 109
YQ K +C+F G A + ++PS + Y
Sbjct: 384 YQKKGQQALACDFAGTATVVTSDPSVSGCAY 476
>BG587358 similar to GP|6041834|gb|A putative glucan
endo-1-3-beta-glucosidase {Arabidopsis thaliana},
partial (21%)
Length = 460
Score = 74.7 bits (182), Expect = 8e-15
Identities = 35/88 (39%), Positives = 50/88 (56%), Gaps = 1/88 (1%)
Frame = -2
Query: 23 GASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYY-Q 81
G +WC+ + A++ ++ AL YAC G C +QPG C+ P+SV HAS+AF+ Y+ Q
Sbjct: 447 GKAWCVVAKGANKTAVVEALSYACSQGNRTCELVQPGKPCFEPDSVVGHASYAFSSYWAQ 268
Query: 82 KNPVPNSCNFGGNAVLTNTNPSKASTIY 109
V +CNF G A +PS S Y
Sbjct: 267 FRRVGGTCNFNGLATQIAEDPSYGSCKY 184
>AL370014 similar to GP|14279169|gb| beta-1 3-glucanase-like protein {Olea
europaea}, partial (13%)
Length = 514
Score = 74.7 bits (182), Expect = 8e-15
Identities = 37/74 (50%), Positives = 50/74 (67%), Gaps = 3/74 (4%)
Frame = +1
Query: 42 LDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKYYQ---KNPVPNSCNFGGNAVLT 98
LDYACG G DCS IQPGG+C+ PN++ +HA++A N +Y +NP+ +C+F A LT
Sbjct: 1 LDYACGQG-IDCSLIQPGGACFEPNTLANHAAYAMNLFYHTVGQNPL--TCDFSQTATLT 171
Query: 99 NTNPSKASTIYDHG 112
+ NPS S IY G
Sbjct: 172 SNNPSYNSCIYTGG 213
>TC87078 similar to PIR|T50645|T50645 glucan endo-1 3-beta-D-glucosidase (EC
3.2.1.39) [imported] - garden pea, partial (94%)
Length = 1628
Score = 73.2 bits (178), Expect = 2e-14
Identities = 36/92 (39%), Positives = 51/92 (55%), Gaps = 1/92 (1%)
Frame = +1
Query: 13 PSTSNSPVSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHA 72
P T P + SWC+ P AS +LQ ++Y C DC IQPGG C+ N++ A
Sbjct: 1072 PVTPVQPRPAIGSWCVPKPDASNAALQANINYVCSQK-VDCRPIQPGGVCFAANNLRALA 1248
Query: 73 SFAFNKYYQ-KNPVPNSCNFGGNAVLTNTNPS 103
++A N YYQ P +C+F + V+ +TNPS
Sbjct: 1249 TYAMNAYYQAMGRHPFNCDFSNSGVIVSTNPS 1344
>TC80310 weakly similar to GP|20160496|dbj|BAB89448. beta-1 3-glucanase-like
protein {Oryza sativa (japonica cultivar-group)},
partial (70%)
Length = 599
Score = 68.2 bits (165), Expect = 7e-13
Identities = 33/91 (36%), Positives = 54/91 (59%), Gaps = 1/91 (1%)
Frame = +1
Query: 20 VSSGASWCIASPSASQRSLQVALDYACGYGGTDCSAIQPGGSCYNPNSVHDHASFAFNKY 79
V+ +WC+A PS+ Q +L L+YAC + DC +Q G C +P ++ + AS A N Y
Sbjct: 175 VNGQKTWCVAKPSSDQATLLSNLNYACSH--VDCRVLQKGCPCSSPENLMNRASIAMNLY 348
Query: 80 YQKNPVPN-SCNFGGNAVLTNTNPSKASTIY 109
Y+ + +C+F G+A++ T+PS + IY
Sbjct: 349 YRSKGTDHWNCDFRGSALVVVTDPSYGNCIY 441
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.125 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,439,458
Number of Sequences: 36976
Number of extensions: 70342
Number of successful extensions: 390
Number of sequences better than 10.0: 74
Number of HSP's better than 10.0 without gapping: 368
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 370
length of query: 120
length of database: 9,014,727
effective HSP length: 84
effective length of query: 36
effective length of database: 5,908,743
effective search space: 212714748
effective search space used: 212714748
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 52 (24.6 bits)
Medicago: description of AC147496.8