BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147483.2 + phase: 0
(384 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79182 similar to GP|15028159|gb|AAK76703.1 unknown protein {Ar... 174 4e-84
TC83098 weakly similar to PIR|T04894|T04894 hypothetical protein... 122 2e-28
TC79183 similar to GP|15028159|gb|AAK76703.1 unknown protein {Ar... 78 8e-22
BI268769 similar to GP|15028159|gb| unknown protein {Arabidopsis... 49 4e-07
AJ500787 34 0.083
TC89235 similar to SP|P57758|CTNS_ARATH Cystinosin homolog. [Mou... 31 0.70
CA990288 weakly similar to GP|15451218|gb Unknown protein {Arabi... 30 2.0
TC76739 similar to GP|18767124|gb|AAL79277.1 unknown {Saccharomy... 28 4.5
CA990124 weakly similar to PIR|A96697|A96 protein F1N21.18 [impo... 28 5.9
CB065289 similar to GP|21647520|gb conserved hypothetical protei... 28 5.9
TC76736 homologue to GP|22725683|gb|AAN04890.1 ribosomal protein... 28 7.7
>TC79182 similar to GP|15028159|gb|AAK76703.1 unknown protein {Arabidopsis
thaliana}, partial (61%)
Length = 1205
Score = 174 bits (440), Expect(2) = 4e-84
Identities = 118/240 (49%), Positives = 145/240 (60%), Gaps = 2/240 (0%)
Frame = +1
Query: 143 *RREKTLESKTKSSVLWDCNTKWHTKRSSSRGVLLHVSKIVSGECNSTLVYPSKSC*KWS 202
*RREKT E+K K V D N KW T+ ++ +LL +SKI+ C ST+ Y +SC*+W+
Sbjct: 313 *RREKTFETKRKI*VR-DSNKKW*TQSNT*TRILLWISKIIGW*CYSTISYLHESC*EWT 489
Query: 203 FSLGIHPRF-F****SISSYVQYLYNQALVHTTFCRWTLWNISSYSH*LALKG*FNEIWI 261
F GI RF F*** + S N A + +FC LWN+SS +*LA * E I
Sbjct: 490 FGNGIERRFIF***GAFGSC-----NTA*TNPSFCG-KLWNVSSSIN*LAPSE*CFESRI 651
Query: 262 HRIYWNKTP*GICCGT*YIWTIFGMDHGCYIHM*SNSSNMVEH*KR*CRGLKSFHVRFCT 321
H W KT C T I ++ GM HGCY++ S++SNMV++ KR C G KSFHV CT
Sbjct: 652 HSTKWKKTTLPGTCNTQCIGSMVGMAHGCYLYRWSDTSNMVKYQKREC*GFKSFHVHLCT 831
Query: 322 DC*YFLRGKYSCTNN*V*KHQSKFAMVVGCHSLRGTRLFYNIAVHLL*ILSQ-Q*IK**W 380
DC* L GKYSC N + KHQS +AMV GCHSL L YNIAVH L I S+ IK* W
Sbjct: 832 DC*CHLCGKYSCKNYRMGKHQS*YAMVAGCHSLCCIGLIYNIAVHQLQIPSKDNNIK*LW 1011
Score = 155 bits (393), Expect(2) = 4e-84
Identities = 71/98 (72%), Positives = 84/98 (85%)
Frame = +2
Query: 30 DDISFSLGLMSLVSWGVAEIPQIITIFRNKSSHGISLAFLLTWVAGDICNLVGCLLEPAT 89
D+ISF+ G +SL+ WGVAEIPQIIT FR KSSHG+S+ FLLTWVAGDI NLVGCLLEPAT
Sbjct: 2 DNISFTFGFISLICWGVAEIPQIITNFRAKSSHGVSIVFLLTWVAGDIFNLVGCLLEPAT 181
Query: 90 LPTQFYTALLYASTTIILLLQIVYYDHILRWCKHRQNV 127
LPTQ+YTALLY TTI+L++Q YYD+I +WCK RQ +
Sbjct: 182 LPTQYYTALLYTITTIVLVVQSFYYDYIYKWCKRRQKI 295
>TC83098 weakly similar to PIR|T04894|T04894 hypothetical protein F18F4.200
- Arabidopsis thaliana, partial (29%)
Length = 467
Score = 122 bits (307), Expect = 2e-28
Identities = 56/96 (58%), Positives = 72/96 (74%)
Frame = +2
Query: 33 SFSLGLMSLVSWGVAEIPQIITIFRNKSSHGISLAFLLTWVAGDICNLVGCLLEPATLPT 92
S +LG++S++ W +AEIPQ+IT +R KSSHG+S+ FLLTW+ GD+ NL GCLLEPATLPT
Sbjct: 170 SITLGVISVIVWMIAEIPQLITNYREKSSHGLSVTFLLTWIIGDLFNLFGCLLEPATLPT 349
Query: 93 QFYTALLYASTTIILLLQIVYYDHILRWCKHRQNVK 128
Q YTA+LY T+ L LQ YY HI KH++ K
Sbjct: 350 QLYTAVLYTLITLTLCLQATYYGHIYPRLKHKRQFK 457
>TC79183 similar to GP|15028159|gb|AAK76703.1 unknown protein {Arabidopsis
thaliana}, partial (23%)
Length = 931
Score = 77.8 bits (190), Expect(2) = 8e-22
Identities = 70/199 (35%), Positives = 94/199 (47%), Gaps = 36/199 (18%)
Frame = +1
Query: 131 LLLLQLYY-----LTG**RREKTLESKTKSSVLWDCNTKWHTKRSSSRGVLL-------- 177
++L+ LYY + *RREKT E+K K V D N KW T+ ++ +LL
Sbjct: 289 IILIILYYYCYVIIIDL*RREKTFETKRKI*VR-DSNKKW*TQSNT*TRILLWVSTHL*L 465
Query: 178 ----------------------HVSKIVSGECNSTLVYPSKSC*KWSFSLGIHPRF-F** 214
+SKI+ C ST+ Y +SC*+W+F GI RF F**
Sbjct: 466 NC*LVD*AIF*TDEF*IN*LSQQISKIIGW*CYSTISYLHESC*EWTFGNGIERRFIF** 645
Query: 215 **SISSYVQYLYNQALVHTTFCRWTLWNISSYSH*LALKG*FNEIWIHRIYWNKTP*GIC 274
* + S N A + +FC LWN+SS +*LA * E IH W KT
Sbjct: 646 *GAFGSC-----NTA*TNPSFCG-KLWNVSSSIN*LAPSE*CFESRIHSTKWKKTTLPGT 807
Query: 275 CGT*YIWTIFGMDHGCYIH 293
C T I ++ GM HGCY++
Sbjct: 808 CNTQCIGSMVGMAHGCYLY 864
Score = 43.5 bits (101), Expect(2) = 8e-22
Identities = 20/40 (50%), Positives = 28/40 (70%), Gaps = 2/40 (5%)
Frame = +2
Query: 99 LYASTTIILLLQIVYYDHILRWCKHRQ--NVKSKLLLLQL 136
LY TTI+L++Q YYD+I +WCK RQ N++ L L Q+
Sbjct: 143 LYTITTIVLVVQSFYYDYIYKWCKRRQKINIEEVLSLNQI 262
>BI268769 similar to GP|15028159|gb| unknown protein {Arabidopsis thaliana},
partial (13%)
Length = 554
Score = 48.9 bits (115), Expect(2) = 4e-07
Identities = 30/52 (57%), Positives = 34/52 (64%), Gaps = 1/52 (1%)
Frame = +2
Query: 330 KYSCTNN*V*KHQSKFAMVVGCHSLRGTRLFYNIAVHLL*ILSQ-Q*IK**W 380
+YSC N + KHQS +AMV GCHSL L YNIAVH L I S+ IK* W
Sbjct: 221 QYSCKNYRMGKHQS*YAMVAGCHSLCCIGLIYNIAVHQLQIPSKDNNIK*LW 376
Score = 22.7 bits (47), Expect(2) = 4e-07
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = +1
Query: 311 GLKSFHVRFCT 321
G KSFHV CT
Sbjct: 61 GFKSFHVHLCT 93
>AJ500787
Length = 566
Score = 34.3 bits (77), Expect = 0.083
Identities = 25/86 (29%), Positives = 37/86 (42%), Gaps = 1/86 (1%)
Frame = +3
Query: 35 SLGLMSLVSWGVAEIPQIITIFRNKSSHGISLAFLLTWVAGDICNLVGCLLEPATLPTQF 94
+LG +SL +PQ F+ S G S +LTWV GD + A P QF
Sbjct: 210 TLGYLSLGIESTVPMPQAYQNFKRHSVSGFSKWIILTWVGGDSFKTFYYIYTHA--PLQF 383
Query: 95 Y-TALLYASTTIILLLQIVYYDHILR 119
++ S +++ Q V Y +R
Sbjct: 384 IPCGIVQLSVDFVIVFQTVVYGRKMR 461
>TC89235 similar to SP|P57758|CTNS_ARATH Cystinosin homolog. [Mouse-ear
cress] {Arabidopsis thaliana}, partial (92%)
Length = 1103
Score = 31.2 bits (69), Expect = 0.70
Identities = 13/35 (37%), Positives = 21/35 (59%)
Frame = +1
Query: 36 LGLMSLVSWGVAEIPQIITIFRNKSSHGISLAFLL 70
LG + + W ++ PQ+I FR KS G++ F+L
Sbjct: 67 LGWFAFIVWSISFYPQVILNFRRKSVVGLNFDFVL 171
>CA990288 weakly similar to GP|15451218|gb Unknown protein {Arabidopsis
thaliana}, partial (46%)
Length = 586
Score = 29.6 bits (65), Expect = 2.0
Identities = 16/48 (33%), Positives = 24/48 (49%)
Frame = +1
Query: 75 GDICNLVGCLLEPATLPTQFYTALLYASTTIILLLQIVYYDHILRWCK 122
G + L CLL P L F LL +++LL++ DH + WC+
Sbjct: 289 G*MMKLAHCLLWPLVLLLDFLVPLLPLLLMVLILLEV---DHNVPWCQ 423
>TC76739 similar to GP|18767124|gb|AAL79277.1 unknown {Saccharomyces
cerevisiae}, partial (29%)
Length = 371
Score = 28.5 bits (62), Expect = 4.5
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -3
Query: 147 KTLESKTKSSVLWDCNTKWHTKRS 170
K ++ +KS V+WDC++KW S
Sbjct: 114 KLYDTFSKSRVVWDCSSKWEVNFS 43
>CA990124 weakly similar to PIR|A96697|A96 protein F1N21.18 [imported] -
Arabidopsis thaliana, partial (72%)
Length = 537
Score = 28.1 bits (61), Expect = 5.9
Identities = 19/51 (37%), Positives = 26/51 (50%)
Frame = +1
Query: 84 LLEPATLPTQFYTALLYASTTIILLLQIVYYDHILRWCKHRQNVKSKLLLL 134
LLEP + + + L +T L L+I + RW K N+KS LLLL
Sbjct: 52 LLEPLKVLSLPFLVLF--TTNSSLFLKISLFFSTTRWMKQPTNLKSMLLLL 198
>CB065289 similar to GP|21647520|gb conserved hypothetical protein
{Chlorobium tepidum TLS}, partial (25%)
Length = 654
Score = 28.1 bits (61), Expect = 5.9
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = -2
Query: 148 TLESKTKSSVLWDCNTKWHTKRSSSRGVLLHVSKI 182
T+ S+T++ V C+T W T+R RG + H++ I
Sbjct: 458 TMVSRTRTRV---CSTCWQTRRPILRGSICHLTPI 363
>TC76736 homologue to GP|22725683|gb|AAN04890.1 ribosomal protein L14 {Vigna
angularis}, partial (38%)
Length = 566
Score = 27.7 bits (60), Expect = 7.7
Identities = 9/21 (42%), Positives = 15/21 (70%)
Frame = -1
Query: 150 ESKTKSSVLWDCNTKWHTKRS 170
++ +KS V+WDC++KW S
Sbjct: 299 DTLSKSRVVWDCSSKWEVNFS 237
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.354 0.155 0.589
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,284,895
Number of Sequences: 36976
Number of extensions: 286635
Number of successful extensions: 3340
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 2480
Number of HSP's successfully gapped in prelim test: 86
Number of HSP's that attempted gapping in prelim test: 817
Number of HSP's gapped (non-prelim): 2634
length of query: 384
length of database: 9,014,727
effective HSP length: 98
effective length of query: 286
effective length of database: 5,391,079
effective search space: 1541848594
effective search space used: 1541848594
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC147483.2