
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147482.11 - phase: 0
(109 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78962 similar to PIR|S38958|S38958 chorismate mutase (EC 5.4.9... 141 5e-53
TC89002 weakly similar to GP|12325088|gb|AAG52497.1 putative cho... 142 2e-35
TC80726 similar to GP|18491975|emb|CAD22399. unnamed protein pro... 98 4e-22
TC87476 homologue to PIR|T11622|T11622 extensin class 1 precurso... 28 0.42
TC80478 similar to GP|15215746|gb|AAK91418.1 At1g12930/F13K23_14... 25 3.5
BQ135870 25 6.0
BQ139391 weakly similar to GP|9758470|dbj| receptor kinase-like ... 24 7.8
TC84239 weakly similar to PIR|A96747|A96747 probable RNA-binding... 24 7.8
>TC78962 similar to PIR|S38958|S38958 chorismate mutase (EC 5.4.99.5)
precursor - Arabidopsis thaliana, partial (75%)
Length = 1318
Score = 141 bits (355), Expect(2) = 5e-53
Identities = 72/72 (100%), Positives = 72/72 (100%)
Frame = +2
Query: 1 ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY 60
ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY
Sbjct: 809 ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY 988
Query: 61 GQEVAVNLKDQK 72
GQEVAVNLKDQK
Sbjct: 989 GQEVAVNLKDQK 1024
Score = 81.3 bits (199), Expect(2) = 5e-53
Identities = 39/41 (95%), Positives = 40/41 (97%)
Frame = +3
Query: 69 KDQKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD 109
K +KTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD
Sbjct: 1014 KIKKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD 1136
>TC89002 weakly similar to GP|12325088|gb|AAG52497.1 putative chorismate
mutase; 16810-15349 {Arabidopsis thaliana}, partial
(42%)
Length = 1028
Score = 142 bits (359), Expect = 2e-35
Identities = 69/109 (63%), Positives = 85/109 (77%)
Frame = +1
Query: 1 ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY 60
ALSKRIHYGKFVAEAKFQ P Y+AAI A+D+ RL+ELLTY VEES+++RV +KA+T+
Sbjct: 448 ALSKRIHYGKFVAEAKFQECPSVYEAAIKAKDRKRLLELLTYESVEESVQKRVEMKARTF 627
Query: 61 GQEVAVNLKDQKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD 109
QEV +N +PVY+I L+ +LY WIMPLTKEVQV YLLR+LD
Sbjct: 628 SQEVKINEAGDVADPVYRIKSGLIGNLYGKWIMPLTKEVQVEYLLRRLD 774
>TC80726 similar to GP|18491975|emb|CAD22399. unnamed protein product
{synthetic construct}, partial (41%)
Length = 872
Score = 98.2 bits (243), Expect = 4e-22
Identities = 45/96 (46%), Positives = 69/96 (71%)
Frame = +1
Query: 1 ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY 60
A+SKR+HYGKFVAE KF+ +P Y+ I ++DK+ LM+LLT+ VEE +K+RV KA +
Sbjct: 487 AISKRVHYGKFVAEVKFRESPQDYEPLIRSKDKEGLMKLLTFKSVEEMVKKRVEKKATLF 666
Query: 61 GQEVAVNLKDQKTEPVYKINPSLVADLYSDWIMPLT 96
GQ++++N D ++ K +PS+ + LY W++PLT
Sbjct: 667 GQDISLNSSDD-SKGKQKFDPSVASKLYEKWVIPLT 771
>TC87476 homologue to PIR|T11622|T11622 extensin class 1 precursor - cowpea,
partial (48%)
Length = 939
Score = 28.5 bits (62), Expect = 0.42
Identities = 22/80 (27%), Positives = 40/80 (49%)
Frame = -1
Query: 24 YKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTYGQEVAVNLKDQKTEPVYKINPSL 83
++A + + +D + E++ EVE + VKAK EVAV E + K+ +
Sbjct: 717 WEAGVSCRGRDDMEEVMEMVEVETCKYKEAVVKAK----EVAVTYSHMVEEVMEKVEVEI 550
Query: 84 VADLYSDWIMPLTKEVQVAY 103
+ + + ++ L KEV+V Y
Sbjct: 549 CSSM--EEVVKL-KEVEVTY 499
>TC80478 similar to GP|15215746|gb|AAK91418.1 At1g12930/F13K23_14
{Arabidopsis thaliana}, partial (27%)
Length = 1388
Score = 25.4 bits (54), Expect = 3.5
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = +2
Query: 73 TEPVYKINPSLVADLYSDWIMP 94
T P + P++V DLY W MP
Sbjct: 548 TRPPFMSYPTVVRDLYLMWCMP 613
>BQ135870
Length = 809
Score = 24.6 bits (52), Expect = 6.0
Identities = 24/74 (32%), Positives = 32/74 (42%)
Frame = -1
Query: 2 LSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTYG 61
LS R+ YGK FQA S+ A K RL+EL ES+ + + YG
Sbjct: 749 LSMRVSYGKTGDGKLFQAKNSSF----CAS*KARLIEL----P*RESLLPQSDLGGGVYG 594
Query: 62 QEVAVNLKDQKTEP 75
Q V + Q+ P
Sbjct: 593 QIVKF*CRPQRCRP 552
>BQ139391 weakly similar to GP|9758470|dbj| receptor kinase-like protein
{Arabidopsis thaliana}, partial (3%)
Length = 622
Score = 24.3 bits (51), Expect = 7.8
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = +2
Query: 79 INPSLVADLYSDWIMPLTKEV 99
+NPSL +DL+ +I L KE+
Sbjct: 230 LNPSLRSDLFRHFIFHLEKEI 292
>TC84239 weakly similar to PIR|A96747|A96747 probable RNA-binding domain
T10D10.21 [imported] - Arabidopsis thaliana, partial
(14%)
Length = 735
Score = 24.3 bits (51), Expect = 7.8
Identities = 22/65 (33%), Positives = 33/65 (49%), Gaps = 5/65 (7%)
Frame = +3
Query: 50 KRRVAVKAKTYGQEVAVNLKDQKT--EPVYKINPSLVADLYSDWIM---PLTKEVQVAYL 104
KRR+ K + + EVA++ T + N SL + S+ + L+K Q +YL
Sbjct: 153 KRRLVAKLQGHFGEVALHSSGAFTIEKCFTACNLSLRETIVSELLAVQSELSKTKQGSYL 332
Query: 105 LRKLD 109
LRKLD
Sbjct: 333 LRKLD 347
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.132 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,326,589
Number of Sequences: 36976
Number of extensions: 18981
Number of successful extensions: 99
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of query: 109
length of database: 9,014,727
effective HSP length: 85
effective length of query: 24
effective length of database: 5,871,767
effective search space: 140922408
effective search space used: 140922408
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)
Medicago: description of AC147482.11