Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC147482.11 - phase: 0 
         (109 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC78962 similar to PIR|S38958|S38958 chorismate mutase (EC 5.4.9...   141  5e-53
TC89002 weakly similar to GP|12325088|gb|AAG52497.1 putative cho...   142  2e-35
TC80726 similar to GP|18491975|emb|CAD22399. unnamed protein pro...    98  4e-22
TC87476 homologue to PIR|T11622|T11622 extensin class 1 precurso...    28  0.42
TC80478 similar to GP|15215746|gb|AAK91418.1 At1g12930/F13K23_14...    25  3.5
BQ135870                                                               25  6.0
BQ139391 weakly similar to GP|9758470|dbj| receptor kinase-like ...    24  7.8
TC84239 weakly similar to PIR|A96747|A96747 probable RNA-binding...    24  7.8

>TC78962 similar to PIR|S38958|S38958 chorismate mutase (EC 5.4.99.5)
           precursor - Arabidopsis thaliana, partial (75%)
          Length = 1318

 Score =  141 bits (355), Expect(2) = 5e-53
 Identities = 72/72 (100%), Positives = 72/72 (100%)
 Frame = +2

Query: 1   ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY 60
           ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY
Sbjct: 809 ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY 988

Query: 61  GQEVAVNLKDQK 72
           GQEVAVNLKDQK
Sbjct: 989 GQEVAVNLKDQK 1024



 Score = 81.3 bits (199), Expect(2) = 5e-53
 Identities = 39/41 (95%), Positives = 40/41 (97%)
 Frame = +3

Query: 69   KDQKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD 109
            K +KTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD
Sbjct: 1014 KIKKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD 1136


>TC89002 weakly similar to GP|12325088|gb|AAG52497.1 putative chorismate
           mutase; 16810-15349 {Arabidopsis thaliana}, partial
           (42%)
          Length = 1028

 Score =  142 bits (359), Expect = 2e-35
 Identities = 69/109 (63%), Positives = 85/109 (77%)
 Frame = +1

Query: 1   ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY 60
           ALSKRIHYGKFVAEAKFQ  P  Y+AAI A+D+ RL+ELLTY  VEES+++RV +KA+T+
Sbjct: 448 ALSKRIHYGKFVAEAKFQECPSVYEAAIKAKDRKRLLELLTYESVEESVQKRVEMKARTF 627

Query: 61  GQEVAVNLKDQKTEPVYKINPSLVADLYSDWIMPLTKEVQVAYLLRKLD 109
            QEV +N      +PVY+I   L+ +LY  WIMPLTKEVQV YLLR+LD
Sbjct: 628 SQEVKINEAGDVADPVYRIKSGLIGNLYGKWIMPLTKEVQVEYLLRRLD 774


>TC80726 similar to GP|18491975|emb|CAD22399. unnamed protein product
           {synthetic construct}, partial (41%)
          Length = 872

 Score = 98.2 bits (243), Expect = 4e-22
 Identities = 45/96 (46%), Positives = 69/96 (71%)
 Frame = +1

Query: 1   ALSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTY 60
           A+SKR+HYGKFVAE KF+ +P  Y+  I ++DK+ LM+LLT+  VEE +K+RV  KA  +
Sbjct: 487 AISKRVHYGKFVAEVKFRESPQDYEPLIRSKDKEGLMKLLTFKSVEEMVKKRVEKKATLF 666

Query: 61  GQEVAVNLKDQKTEPVYKINPSLVADLYSDWIMPLT 96
           GQ++++N  D  ++   K +PS+ + LY  W++PLT
Sbjct: 667 GQDISLNSSDD-SKGKQKFDPSVASKLYEKWVIPLT 771


>TC87476 homologue to PIR|T11622|T11622 extensin class 1 precursor - cowpea,
           partial (48%)
          Length = 939

 Score = 28.5 bits (62), Expect = 0.42
 Identities = 22/80 (27%), Positives = 40/80 (49%)
 Frame = -1

Query: 24  YKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTYGQEVAVNLKDQKTEPVYKINPSL 83
           ++A +  + +D + E++   EVE    +   VKAK    EVAV       E + K+   +
Sbjct: 717 WEAGVSCRGRDDMEEVMEMVEVETCKYKEAVVKAK----EVAVTYSHMVEEVMEKVEVEI 550

Query: 84  VADLYSDWIMPLTKEVQVAY 103
            + +  + ++ L KEV+V Y
Sbjct: 549 CSSM--EEVVKL-KEVEVTY 499


>TC80478 similar to GP|15215746|gb|AAK91418.1 At1g12930/F13K23_14
           {Arabidopsis thaliana}, partial (27%)
          Length = 1388

 Score = 25.4 bits (54), Expect = 3.5
 Identities = 10/22 (45%), Positives = 13/22 (58%)
 Frame = +2

Query: 73  TEPVYKINPSLVADLYSDWIMP 94
           T P +   P++V DLY  W MP
Sbjct: 548 TRPPFMSYPTVVRDLYLMWCMP 613


>BQ135870 
          Length = 809

 Score = 24.6 bits (52), Expect = 6.0
 Identities = 24/74 (32%), Positives = 32/74 (42%)
 Frame = -1

Query: 2   LSKRIHYGKFVAEAKFQAAPDSYKAAIIAQDKDRLMELLTYPEVEESIKRRVAVKAKTYG 61
           LS R+ YGK      FQA   S+     A  K RL+EL       ES+  +  +    YG
Sbjct: 749 LSMRVSYGKTGDGKLFQAKNSSF----CAS*KARLIEL----P*RESLLPQSDLGGGVYG 594

Query: 62  QEVAVNLKDQKTEP 75
           Q V    + Q+  P
Sbjct: 593 QIVKF*CRPQRCRP 552


>BQ139391 weakly similar to GP|9758470|dbj| receptor kinase-like protein
           {Arabidopsis thaliana}, partial (3%)
          Length = 622

 Score = 24.3 bits (51), Expect = 7.8
 Identities = 10/21 (47%), Positives = 15/21 (70%)
 Frame = +2

Query: 79  INPSLVADLYSDWIMPLTKEV 99
           +NPSL +DL+  +I  L KE+
Sbjct: 230 LNPSLRSDLFRHFIFHLEKEI 292


>TC84239 weakly similar to PIR|A96747|A96747 probable RNA-binding domain
           T10D10.21 [imported] - Arabidopsis thaliana, partial
           (14%)
          Length = 735

 Score = 24.3 bits (51), Expect = 7.8
 Identities = 22/65 (33%), Positives = 33/65 (49%), Gaps = 5/65 (7%)
 Frame = +3

Query: 50  KRRVAVKAKTYGQEVAVNLKDQKT--EPVYKINPSLVADLYSDWIM---PLTKEVQVAYL 104
           KRR+  K + +  EVA++     T  +     N SL   + S+ +     L+K  Q +YL
Sbjct: 153 KRRLVAKLQGHFGEVALHSSGAFTIEKCFTACNLSLRETIVSELLAVQSELSKTKQGSYL 332

Query: 105 LRKLD 109
           LRKLD
Sbjct: 333 LRKLD 347


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.316    0.132    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,326,589
Number of Sequences: 36976
Number of extensions: 18981
Number of successful extensions: 99
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of query: 109
length of database: 9,014,727
effective HSP length: 85
effective length of query: 24
effective length of database: 5,871,767
effective search space: 140922408
effective search space used: 140922408
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)


Medicago: description of AC147482.11