BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147435.10 + phase: 0 /pseudo
(612 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90516 114 1e-25
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 90 2e-18
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 85 7e-17
BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [impo... 79 7e-15
BE248682 similar to GP|18568269|gb putative gag-pol polyprotein ... 72 8e-13
BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At... 48 6e-10
BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - ... 59 7e-09
BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-li... 56 4e-08
AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase... 40 0.002
TC83634 weakly similar to GP|11036868|gb|AAG27371.1 PxORF73 pept... 29 6.0
TC85605 similar to GP|437310|gb|AAA62850.1|| nodulin {Medicago t... 28 7.9
>TC90516
Length = 983
Score = 114 bits (285), Expect = 1e-25
Identities = 61/147 (41%), Positives = 91/147 (61%)
Frame = -2
Query: 3 VNFNRLSEVDNDLLVAPFHMEEIEVVVRESDGNKSPGADEYNFAFVKEFWYLMKNEVRIM 62
++F +S +DN LL A F + ++E+VV DGN+SP D +N F ++K ++ IM
Sbjct: 571 IDFFHISNLDNVLLSAQFLVSKMELVVSSLDGNESPRPDGFNLNFFIRLRNMLKADIEIM 392
Query: 63 FDQIHANELLPKSMLAYFVALIPKVSSPLELKDYRTISLLGSLYKLLAKVLARRLAGVLS 122
F+Q + L K YF+ LI KV P+ L D+ +S LGSL KL+AKVLA RLA ++
Sbjct: 391 FEQFYTPANLLKIFS*YFLTLISKVEYPILLGDFSLMSFLGSL*KLMAKVLALRLAHIME 212
Query: 123 SVISTTQSAFLKGTNLVDGVLVVNELV 149
+I QS F++G VDGV+ +NE++
Sbjct: 211 KIIFVNQSTFVRGRQHVDGVVAINEII 131
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 90.1 bits (222), Expect = 2e-18
Identities = 48/142 (33%), Positives = 82/142 (56%), Gaps = 1/142 (0%)
Frame = +3
Query: 14 DLLVAPFHMEEIEVVVRESDGNKSPGADEYNFAFVKEFWYLMKNEVRIMFDQIHANELLP 73
DLL + F E++ + D +K+PG D YN F K W ++ + V +P
Sbjct: 6 DLLCSEFTAVEVKNALFSMDSSKAPGIDGYNVHFFKCSWNIIGDSVIDAILDFFKTGFMP 185
Query: 74 KSMLAYFVALIPKVSSPLELKDYRTISLLGSLYKLLAKVLARRLAGVLSSVISTTQSAFL 133
K + +V L+PK + +K++R I+ +YK+++K+L R+ GVL+SV+S QSAF+
Sbjct: 186 KIINCTYVTLLPKEVNVTSVKNFRPIACCSVIYKIISKILTSRMQGVLNSVVSENQSAFV 365
Query: 134 KGTNLVDGVLVVNELV-DYAKK 154
KG + D +++ +ELV Y++K
Sbjct: 366 KGRVIFDNIILSHELVKSYSRK 431
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 85.1 bits (209), Expect = 7e-17
Identities = 49/115 (42%), Positives = 71/115 (61%)
Frame = +2
Query: 65 QIHANELLPKSMLAYFVALIPKVSSPLELKDYRTISLLGSLYKLLAKVLARRLAGVLSSV 124
+ H N L K + + F+ALIPKV +P L D+R ISL+GSLYK+L K+LA RL V+ SV
Sbjct: 17 EFHRNRKLFKGINSTFIALIPKVDNPQRLNDFRPISLVGSLYKILGKLLANRLRVVIGSV 196
Query: 125 ISTTQSAFLKGTNLVDGVLVVNELVDYAKKS*KKIVSFSRLILRRHMIP*SGVFW 179
IS QSAF+K +++ V + + ++*+KI R IL+R + G+ W
Sbjct: 197 ISDAQSAFVKNRQILEMVFL*QMRLWMRLRN*RKIFCCLRWILKRLITLSIGLIW 361
>BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 687
Score = 78.6 bits (192), Expect = 7e-15
Identities = 42/125 (33%), Positives = 68/125 (53%)
Frame = +2
Query: 20 FHMEEIEVVVRESDGNKSPGADEYNFAFVKEFWYLMKNEVRIMFDQIHANELLPKSMLAY 79
F EE+ + + K+PG D F +++W+++ EV+ M Q+ N + + +
Sbjct: 257 FTEEEVLEAINQMHPVKAPGPDGLPALFFQKYWHIVGKEVQQMVLQVLNNSMETEELNKT 436
Query: 80 FVALIPKVSSPLELKDYRTISLLGSLYKLLAKVLARRLAGVLSSVISTTQSAFLKGTNLV 139
F+ LIPK +P KDYR ISL + K++ KV+A R+ L VI QSAF++G +
Sbjct: 437 FIVLIPKGKNPNTPKDYRPISLCNVVMKIITKVIANRVKQTLPDVIDVEQSAFVQGRLIT 616
Query: 140 DGVLV 144
D L+
Sbjct: 617 DNALI 631
>BE248682 similar to GP|18568269|gb putative gag-pol polyprotein {Zea mays},
partial (1%)
Length = 441
Score = 71.6 bits (174), Expect = 8e-13
Identities = 61/132 (46%), Positives = 70/132 (52%)
Frame = +1
Query: 214 RRRFVFKEALNKVIL*PRSYFCWLRKGLVV**GMRWI*IYSKVLRWEEMVWWCLICNMPM 273
RRR V K NK I * F RK LVV** MR I I SKVL E LI NMPM
Sbjct: 4 RRRLVSKGV*NKEIR*HLFCFFLWRKELVV**RMR*IVICSKVLMSREEERVSLIFNMPM 183
Query: 274 TLFVLGKLPWRIFGR*RLF*EVLKWLPG*RLIFPKALL*ELIFRRILCKWRAIF*IVVKG 333
LFV G IFG +L+ +VLKWLP *+L +AL * +F + R *IV +
Sbjct: 184 ILFV*GCRRLTIFGLLKLYCKVLKWLPD*KLTSIRAL**VSMFLGTSWRRRVDS*IVERS 363
Query: 334 VSRSSIWDYQWG 345
+ R WDY G
Sbjct: 364 LFRLFTWDYPVG 399
>BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (16%)
Length = 794
Score = 47.8 bits (112), Expect(2) = 6e-10
Identities = 24/64 (37%), Positives = 40/64 (62%)
Frame = +1
Query: 83 LIPKVSSPLELKDYRTISLLGSLYKLLAKVLARRLAGVLSSVISTTQSAFLKGTNLVDGV 142
L+PK L ++R ISL YK+++KVL++RL VL +I+ TQ+AF + + D +
Sbjct: 601 LVPKKLEAKRLVEFRPISLCNVAYKIVSKVLSKRLKSVLPWIITETQAAFGRRQLISDNI 780
Query: 143 LVVN 146
L+ +
Sbjct: 781 LIAH 792
Score = 34.3 bits (77), Expect(2) = 6e-10
Identities = 17/55 (30%), Positives = 29/55 (51%)
Frame = +3
Query: 8 LSEVDNDLLVAPFHMEEIEVVVRESDGNKSPGADEYNFAFVKEFWYLMKNEVRIM 62
++E N L+A EE+ V + + +K PG D N F ++FW M +++ M
Sbjct: 375 VTEEQNAQLMAQISREEVREAVFDINPHKCPGPDGMNVFFFQQFWDTMGDDLTSM 539
>BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - Arabidopsis
thaliana, partial (17%)
Length = 677
Score = 58.5 bits (140), Expect = 7e-09
Identities = 34/97 (35%), Positives = 53/97 (54%)
Frame = +3
Query: 39 GADEYNFAFVKEFWYLMKNEVRIMFDQIHANELLPKSMLAYFVALIPKVSSPLELKDYRT 98
G E +F + W+++K ++ M + A+ L + + LIPK P + + R
Sbjct: 387 GRME*QLSFFQHSWHIIKMDLLKMVNSFLASGKLDTRLNTTNICLIPKKKRPTRMTELRP 566
Query: 99 ISLLGSLYKLLAKVLARRLAGVLSSVISTTQSAFLKG 135
ISL YK+++KVL +RL L S+IS TQSAF+ G
Sbjct: 567 ISLCNVGYKIISKVLCQRLKVCLPSLISETQSAFVHG 677
>BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (18%)
Length = 789
Score = 56.2 bits (134), Expect = 4e-08
Identities = 24/63 (38%), Positives = 46/63 (72%)
Frame = -3
Query: 93 LKDYRTISLLGSLYKLLAKVLARRLAGVLSSVISTTQSAFLKGTNLVDGVLVVNELVDYA 152
+ +YRTI+ + YK++AK+L++R+ +L S+IS +QSAF+ G + D VL+ ++++ Y
Sbjct: 778 VSEYRTIAPCNTQYKIIAKILSKRMQPLLRSIISPSQSAFVPGRAISDNVLITHKILHYL 599
Query: 153 KKS 155
++S
Sbjct: 598 RQS 590
>AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (4%)
Length = 305
Score = 40.4 bits (93), Expect = 0.002
Identities = 23/60 (38%), Positives = 31/60 (51%), Gaps = 2/60 (3%)
Frame = +1
Query: 17 VAPFHMEEIEVVVRESDGNKS--PGADEYNFAFVKEFWYLMKNEVRIMFDQIHANELLPK 74
V F EE+ V + D + S PG D NF FVKE+W L+K + + + H N L K
Sbjct: 112 VLDFSEEEVRKAVWDCDSSHS*NPGPDGVNFTFVKEYWELIKVDFLRVVMEFHTNGKLGK 291
>TC83634 weakly similar to GP|11036868|gb|AAG27371.1 PxORF73 peptide
{Plutella xylostella granulovirus}, partial (29%)
Length = 163
Score = 28.9 bits (63), Expect = 6.0
Identities = 20/52 (38%), Positives = 25/52 (47%)
Frame = +2
Query: 551 GGYGGGLWGDFGGELGLEFYLEAKTICMGGGVAY*PFGRFGGNKMVR*GG*V 602
GGYGGG+ GG +G Y +GGGV G GG + GG*+
Sbjct: 20 GGYGGGISNGIGGGIGGSAYKG-----IGGGV-----GGIGGGVIGGVGG*I 145
>TC85605 similar to GP|437310|gb|AAA62850.1|| nodulin {Medicago truncatula},
partial (62%)
Length = 1077
Score = 28.5 bits (62), Expect = 7.9
Identities = 25/111 (22%), Positives = 48/111 (42%), Gaps = 2/111 (1%)
Frame = -2
Query: 215 RRFVFKEALNKVIL*PRSYFCWLRKGLVV**GMRWI*IYSKVLRWEEMVWWCLICNMPMT 274
RRF+++ +N+ +L + W +V W +Y + ++W + WW +
Sbjct: 647 RRFLYRWLVNRQLLNWWLMYRWFLNWWLV----HWRLLYWRFVKWGLLNWWLVHRRFLYR 480
Query: 275 LFVLGKLP--WRIFGR*RLF*EVLKWLPG*RLIFPKALL*ELIFRRILCKW 323
V +L W ++ +* V +W L+ + L L++RR L W
Sbjct: 479 WLVNRQLLN*WFVYRWFLNW*LVYRWFLNWWLVHWRLLNWWLVYRRFLYMW 327
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.358 0.165 0.605
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,802,757
Number of Sequences: 36976
Number of extensions: 307118
Number of successful extensions: 3201
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1329
Number of HSP's successfully gapped in prelim test: 135
Number of HSP's that attempted gapping in prelim test: 1778
Number of HSP's gapped (non-prelim): 1578
length of query: 612
length of database: 9,014,727
effective HSP length: 102
effective length of query: 510
effective length of database: 5,243,175
effective search space: 2674019250
effective search space used: 2674019250
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC147435.10