Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC147406.5 - phase: 0 /pseudo
         (99 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BE248449 similar to GP|12330687|gb 2-isopropylmalate synthase {A...    31  0.068
AL368496 similar to GP|16612268|gb| AT3g61860/F21F14_30 {Arabido...    29  0.26
TC78771 similar to GP|16612268|gb|AAL27502.1 AT3g61860/F21F14_30...    29  0.26
TC79742 similar to GP|10177241|dbj|BAB10615. gb|AAC98455.1~gene_...    25  4.9
TC83797 similar to GP|21618159|gb|AAM67209.1 probable riboflavin...    25  6.4
TC92387                                                                25  6.4
TC84051                                                                24  8.3
BQ751034 similar to GP|18307453|e probable DNA-directed RNA poly...    24  8.3

>BE248449 similar to GP|12330687|gb 2-isopropylmalate synthase {Arabidopsis
           thaliana}, partial (18%)
          Length = 344

 Score = 31.2 bits (69), Expect = 0.068
 Identities = 18/50 (36%), Positives = 29/50 (58%)
 Frame = +2

Query: 33  LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
           +LGK  G HAMR+RL++      G+E+ D+       +F   A+++K IT
Sbjct: 56  VLGKLSGRHAMRKRLEEL-----GYELKDDQVESLFWRFKAVAEQKKRIT 190


>AL368496 similar to GP|16612268|gb| AT3g61860/F21F14_30 {Arabidopsis
           thaliana}, partial (36%)
          Length = 616

 Score = 29.3 bits (64), Expect = 0.26
 Identities = 15/55 (27%), Positives = 26/55 (47%)
 Frame = -2

Query: 37  NLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIFDH 91
           NL Y   +  L++ +   G  E +D  +GF  V +++  D E+ I     + F H
Sbjct: 585 NLEYDTRQSELERLFSKYGRIERVDMKSGFAFVYYEDERDAEEAIRALDNIPFGH 421


>TC78771 similar to GP|16612268|gb|AAL27502.1 AT3g61860/F21F14_30
           {Arabidopsis thaliana}, partial (87%)
          Length = 1254

 Score = 29.3 bits (64), Expect = 0.26
 Identities = 15/55 (27%), Positives = 26/55 (47%)
 Frame = +1

Query: 37  NLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVITGGPWLIFDH 91
           NL Y   +  L++ +   G  E +D  +GF  V +++  D E+ I     + F H
Sbjct: 109 NLEYDTRQSELERLFSKYGRIERVDMKSGFAFVYYEDERDAEEAIRALDNIPFGH 273


>TC79742 similar to GP|10177241|dbj|BAB10615.
           gb|AAC98455.1~gene_id:MRN17.17~strong similarity to
           unknown protein {Arabidopsis thaliana}, partial (32%)
          Length = 1063

 Score = 25.0 bits (53), Expect = 4.9
 Identities = 13/56 (23%), Positives = 30/56 (53%), Gaps = 6/56 (10%)
 Frame = +2

Query: 1   MRMEIVFYPRFI---SNNQSFNNYVRHGRMQFVVKLLGKNLGYH---AMRERLQKT 50
           + + + F+  ++   +NN + NN ++H ++   +K L ++L      +  + LQKT
Sbjct: 317 LSLSLYFFTSYLISNNNNHNHNNNIKHHQITSTIKSLSQSLSIKKTTSSPQTLQKT 484


>TC83797 similar to GP|21618159|gb|AAM67209.1 probable riboflavin
           biosynthesis related protein {Arabidopsis thaliana},
           partial (44%)
          Length = 842

 Score = 24.6 bits (52), Expect = 6.4
 Identities = 16/37 (43%), Positives = 19/37 (51%)
 Frame = +2

Query: 61  DNDNGFYMVKFDEAADKEKVITGGPWLIFDHCLAVTH 97
           DND+GFYM K  E A K    T    L+   C+ V H
Sbjct: 137 DNDDGFYMRKSVELARKGLGYTSPNPLV--GCVIVKH 241


>TC92387 
          Length = 713

 Score = 24.6 bits (52), Expect = 6.4
 Identities = 16/75 (21%), Positives = 31/75 (41%), Gaps = 1/75 (1%)
 Frame = +2

Query: 13  SNNQSFNNYVRHGRMQFVVKLLGKNLG-YHAMRERLQKTWRTQGGFEIMDNDNGFYMVKF 71
           S   S+   +   R++ V +LL       + M ++++  W    G +I    +G Y   F
Sbjct: 155 SRRISYTTIIACPRLRLVGRLLTNRPN*SYVMIKKIEAFWSLVKGVKIQKI*SGLYTF*F 334

Query: 72  DEAADKEKVITGGPW 86
               D + ++   PW
Sbjct: 335 HHHLDLQSILKKCPW 379


>TC84051 
          Length = 818

 Score = 24.3 bits (51), Expect = 8.3
 Identities = 13/35 (37%), Positives = 16/35 (45%)
 Frame = -1

Query: 22  VRHGRMQFVVKLLGKNLGYHAMRERLQKTWRTQGG 56
           VR    QFVV   G+   Y   R R +  W+ Q G
Sbjct: 764 VRPHLRQFVVPASGRGTMYQQRRPRPRCAWKPQRG 660


>BQ751034 similar to GP|18307453|e probable DNA-directed RNA polymerase I
           {Neurospora crassa}, partial (13%)
          Length = 791

 Score = 24.3 bits (51), Expect = 8.3
 Identities = 10/20 (50%), Positives = 12/20 (60%)
 Frame = +3

Query: 23  RHGRMQFVVKLLGKNLGYHA 42
           RHGR   VV+  G   G+HA
Sbjct: 504 RHGRADCVVRFHGPRRGWHA 563


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.327    0.141    0.460 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,165,918
Number of Sequences: 36976
Number of extensions: 33235
Number of successful extensions: 222
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 222
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of query: 99
length of database: 9,014,727
effective HSP length: 75
effective length of query: 24
effective length of database: 6,241,527
effective search space: 149796648
effective search space used: 149796648
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 50 (23.9 bits)


Medicago: description of AC147406.5