BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147364.11 - phase: 0
(184 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87506 similar to GP|9843655|emb|CAC03601.1 SC35-like splicing ... 261 1e-70
BI264830 similar to SP|P51279|PSAD_ Photosystem I reaction cente... 30 0.71
TC90301 similar to GP|14194175|gb|AAK56282.1 AT5g58470/mqj2_60 {... 30 0.71
TC92045 29 1.2
TC77881 homologue to GP|15528439|emb|CAC69137. MEK map kinase ki... 28 1.6
BQ750920 similar to GP|15811426|gb| glycine-rich protein {Coccid... 28 2.1
TC80200 similar to GP|21928041|gb|AAM78049.1 At2g01270/F10A8.15 ... 28 2.7
BQ752223 28 2.7
AJ501289 similar to GP|17529106|gb putative clathrin coat assemb... 28 2.7
BE325816 weakly similar to GP|15028133|gb| unknown protein {Arab... 28 2.7
TC78263 weakly similar to PIR|B85042|B85042 probable chloroplast... 27 3.5
TC81789 similar to PIR|B86440|B86440 probable protein kinase [im... 27 4.6
TC82471 homologue to SP|P07038|PMA1_NEUCR Plasma membrane ATPase... 27 4.6
TC78935 similar to GP|6760451|gb|AAF28357.1| S-ribonuclease bind... 27 4.6
BQ137309 similar to GP|19386767|dbj contains ESTs AU163700(E4234... 27 4.6
BF004047 weakly similar to GP|15027957|gb| putative arginine/ser... 27 6.0
TC78961 similar to GP|18252179|gb|AAL61922.1 unknown protein {Ar... 27 6.0
TC89843 similar to PIR|E85430|E85430 hypothetical protein AT4g36... 27 6.0
BQ141665 similar to GP|18568267|gb putative polyprotein {Zea may... 26 7.8
>TC87506 similar to GP|9843655|emb|CAC03601.1 SC35-like splicing factor
SCL28 28 kD {Arabidopsis thaliana}, partial (51%)
Length = 1558
Score = 261 bits (666), Expect = 1e-70
Identities = 129/129 (100%), Positives = 129/129 (100%)
Frame = +1
Query: 34 VGMIIPQSDQDLILGLSLPVMGRMTGPLVPRMTEVPLVPRMAEGLLAPRMMEGPLVPRTT 93
VGMIIPQSDQDLILGLSLPVMGRMTGPLVPRMTEVPLVPRMAEGLLAPRMMEGPLVPRTT
Sbjct: 811 VGMIIPQSDQDLILGLSLPVMGRMTGPLVPRMTEVPLVPRMAEGLLAPRMMEGPLVPRTT 990
Query: 94 EGPLMPRTMEGLLVPRTTEGPLVSRTREGPPVPRRMAEVQLMRRIWCLADLQAPGVMIAV 153
EGPLMPRTMEGLLVPRTTEGPLVSRTREGPPVPRRMAEVQLMRRIWCLADLQAPGVMIAV
Sbjct: 991 EGPLMPRTMEGLLVPRTTEGPLVSRTREGPPVPRRMAEVQLMRRIWCLADLQAPGVMIAV 1170
Query: 154 LQGLVHGHT 162
LQGLVHGHT
Sbjct: 1171LQGLVHGHT 1197
Score = 69.3 bits (168), Expect = 8e-13
Identities = 50/126 (39%), Positives = 60/126 (46%), Gaps = 5/126 (3%)
Frame = +3
Query: 1 MRVTTSHGSGRSGGGGSRRRARSRSPRRSRYRWVGMIIPQSDQDLILGLS----LPVMGR 56
MRVTTSHGSGRSGGGGSRRRARSRSPRRSRYR + D G P R
Sbjct: 663 MRVTTSHGSGRSGGGGSRRRARSRSPRRSRYRSYSKSPSPARDDSRDGRGRDDYSPKRSR 842
Query: 57 MTGPLV-PRMTEVPLVPRMAEGLLAPRMMEGPLVPRTTEGPLMPRTMEGLLVPRTTEGPL 115
V P + PR + +PR +G PR + PR + P +
Sbjct: 843 SYSRSVSPGDGKDDRSPRAKDDRSSPRAKDGGRSPRAKDDGRSPRAKDDGRSPHAKDNGR 1022
Query: 116 VSRTRE 121
RT++
Sbjct: 1023SPRTKD 1040
>BI264830 similar to SP|P51279|PSAD_ Photosystem I reaction center subunit II
(Photosystem I 16 kDa polypeptide) (PSI-D)., partial
(51%)
Length = 394
Score = 29.6 bits (65), Expect = 0.71
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = -3
Query: 9 SGRSGGGGSRRRARSRSPRRSRYR 32
SGR GG G RR RSP R R+R
Sbjct: 197 SGRCGGPGCCRRCGPRSPSRGRHR 126
>TC90301 similar to GP|14194175|gb|AAK56282.1 AT5g58470/mqj2_60 {Arabidopsis
thaliana}, partial (46%)
Length = 1222
Score = 29.6 bits (65), Expect = 0.71
Identities = 13/26 (50%), Positives = 14/26 (53%)
Frame = +1
Query: 6 SHGSGRSGGGGSRRRARSRSPRRSRY 31
+HG R G GG RRR S RR Y
Sbjct: 769 NHGGNRGGYGGDRRRDNSGPDRRDHY 846
>TC92045
Length = 873
Score = 28.9 bits (63), Expect = 1.2
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +1
Query: 7 HGSGRSGGGGSRRRARSRSPRRSRYRWVGMIIPQSDQDLILGLSLPVM 54
HG+G GGG RRR PRR+ + + L+L S PV+
Sbjct: 31 HGAGDEEGGGGRRRPTLAPPRRNFFSVFFLKFLYQKFHLLLLYSNPVL 174
>TC77881 homologue to GP|15528439|emb|CAC69137. MEK map kinase kinsae
{Medicago sativa subsp. x varia}, complete
Length = 1546
Score = 28.5 bits (62), Expect = 1.6
Identities = 18/55 (32%), Positives = 27/55 (48%)
Frame = +2
Query: 5 TSHGSGRSGGGGSRRRARSRSPRRSRYRWVGMIIPQSDQDLILGLSLPVMGRMTG 59
TS GS + G + +R P+R R + + +PQ D +L + L LP G G
Sbjct: 245 TSTGSATASGSPNNNNSR---PQRRRRDHLTLPLPQRDTNLAVPLPLPPSGGSGG 400
>BQ750920 similar to GP|15811426|gb| glycine-rich protein {Coccidioides
immitis}, partial (18%)
Length = 729
Score = 28.1 bits (61), Expect = 2.1
Identities = 27/83 (32%), Positives = 40/83 (47%), Gaps = 4/83 (4%)
Frame = +1
Query: 1 MRVTTSHGSGRSGGGGSRRRARSRSPRRSRYRWVGMIIPQSDQDLIL---GLSLPVMGRM 57
++++ S G+G SGGGG RR SRS R R I L+L GL + ++G +
Sbjct: 397 LQLSGSGGNG-SGGGGGRRGGSSRSGGRGRSSLGSGISGAVGLGLLLGGVGLLVRLVGAV 573
Query: 58 TGPLVPRMTEVPLVP-RMAEGLL 79
G L + L + +GLL
Sbjct: 574 DGNLDSDLAAFDLFSVHLVDGLL 642
>TC80200 similar to GP|21928041|gb|AAM78049.1 At2g01270/F10A8.15
{Arabidopsis thaliana}, partial (43%)
Length = 1069
Score = 27.7 bits (60), Expect = 2.7
Identities = 13/32 (40%), Positives = 22/32 (68%), Gaps = 2/32 (6%)
Frame = -2
Query: 1 MRVTTSHGSGRSGGGG--SRRRARSRSPRRSR 30
+ +T +G+ GGGG SRRR R+R+ ++S+
Sbjct: 378 VNLTKDRTTGKRGGGGGSSRRRNRNRAQKQSK 283
>BQ752223
Length = 751
Score = 27.7 bits (60), Expect = 2.7
Identities = 15/25 (60%), Positives = 17/25 (68%), Gaps = 3/25 (12%)
Frame = -1
Query: 12 SGGGG--SRRRARSRSP-RRSRYRW 33
SGG G +RRR R+R P RR R RW
Sbjct: 328 SGGSGHSTRRRVRAREPRRRQRRRW 254
>AJ501289 similar to GP|17529106|gb putative clathrin coat assembly protein
{Arabidopsis thaliana}, complete
Length = 636
Score = 27.7 bits (60), Expect = 2.7
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +1
Query: 14 GGGSRRRARSRSPRRSRYR 32
GG RR RS +PRR RYR
Sbjct: 10 GGDYRRLVRSETPRRHRYR 66
>BE325816 weakly similar to GP|15028133|gb| unknown protein {Arabidopsis
thaliana}, partial (7%)
Length = 673
Score = 27.7 bits (60), Expect = 2.7
Identities = 21/65 (32%), Positives = 29/65 (44%), Gaps = 3/65 (4%)
Frame = +3
Query: 35 GMIIPQSDQDLILGLSLPVMGRMTGPL--VPRMTEVPLVPRMAEG-LLAPRMMEGPLVPR 91
G I P + +G+ LP+MG GPL P PL M+ G ++P + P P
Sbjct: 411 GRIRPPGRDNQQVGMQLPMMGSPYGPLGMPPPGPMQPLSHGMSPGPPISPGVFMSPFNPS 590
Query: 92 TTEGP 96
GP
Sbjct: 591 VWPGP 605
>TC78263 weakly similar to PIR|B85042|B85042 probable chloroplast protein
import component [imported] - Arabidopsis thaliana,
partial (41%)
Length = 1057
Score = 27.3 bits (59), Expect = 3.5
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +2
Query: 4 TTSHGSGRSGGGGSRRRARSRSPRRSRYRWVGMIIPQSDQ 43
T +HG G SRR + S P Y W G IP + +
Sbjct: 269 TDNHGHLSLGAPISRRSSSSTRPEAYIYHWSGFRIPANSE 388
>TC81789 similar to PIR|B86440|B86440 probable protein kinase [imported] -
Arabidopsis thaliana, partial (54%)
Length = 1189
Score = 26.9 bits (58), Expect = 4.6
Identities = 16/46 (34%), Positives = 24/46 (51%)
Frame = -2
Query: 88 LVPRTTEGPLMPRTMEGLLVPRTTEGPLVSRTREGPPVPRRMAEVQ 133
L P GPL ++ L + + TE P +R + PP PRR+ + Q
Sbjct: 978 LPPIQATGPLGA*WVDPLDLRKHTE*PSRARHQGSPPAPRRIVDRQ 841
>TC82471 homologue to SP|P07038|PMA1_NEUCR Plasma membrane ATPase (EC
3.6.3.6) (Proton pump). {Neurospora crassa}, partial
(19%)
Length = 780
Score = 26.9 bits (58), Expect = 4.6
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 6/40 (15%)
Frame = -2
Query: 9 SGRSGGGGSR--RRARSRSPRRSRY----RWVGMIIPQSD 42
S R GGGSR RR R RRSRY RW + P+++
Sbjct: 467 SQRCPGGGSRHRRRCRRHRRRRSRYGRRQRWRSVHRPRTN 348
>TC78935 similar to GP|6760451|gb|AAF28357.1| S-ribonuclease binding
protein SBP1 {Petunia x hybrida}, partial (93%)
Length = 1304
Score = 26.9 bits (58), Expect = 4.6
Identities = 14/23 (60%), Positives = 14/23 (60%)
Frame = -3
Query: 6 SHGSGRSGGGGSRRRARSRSPRR 28
SHG G GGGG RRR RS R
Sbjct: 63 SHGGG--GGGGRRRRREERSLSR 1
>BQ137309 similar to GP|19386767|dbj contains ESTs AU163700(E4234)
C98924(E3256) AU163699(E4234) AU162188(E3256)~unknown
protein, partial (8%)
Length = 1032
Score = 26.9 bits (58), Expect = 4.6
Identities = 17/30 (56%), Positives = 19/30 (62%), Gaps = 3/30 (10%)
Frame = -3
Query: 6 SHGSGRSGGG-GSRR--RARSRSPRRSRYR 32
+HG G SGGG G RR R R R+ RSR R
Sbjct: 802 AHGDGASGGGRGVRRPQRVRGRTRVRSRPR 713
>BF004047 weakly similar to GP|15027957|gb| putative arginine/serine-rich
protein {Arabidopsis thaliana}, partial (11%)
Length = 835
Score = 26.6 bits (57), Expect = 6.0
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = +1
Query: 2 RVTTSHGSGRSGGGGSRRRARSRSPRRS 29
R T +SGGGGS R+ +RS R++
Sbjct: 331 RKPTKKEEKKSGGGGSSRKPTTRSQRKA 414
>TC78961 similar to GP|18252179|gb|AAL61922.1 unknown protein {Arabidopsis
thaliana}, partial (71%)
Length = 974
Score = 26.6 bits (57), Expect = 6.0
Identities = 17/30 (56%), Positives = 18/30 (59%)
Frame = +2
Query: 3 VTTSHGSGRSGGGGSRRRARSRSPRRSRYR 32
V+ S G RS SR ARSRSPRR R R
Sbjct: 506 VSRSPGRSRSPAR-SRSPARSRSPRRGRSR 592
>TC89843 similar to PIR|E85430|E85430 hypothetical protein AT4g36470
[imported] - Arabidopsis thaliana, partial (54%)
Length = 940
Score = 26.6 bits (57), Expect = 6.0
Identities = 11/30 (36%), Positives = 16/30 (52%)
Frame = -1
Query: 92 TTEGPLMPRTMEGLLVPRTTEGPLVSRTRE 121
T GPL+PR + + P T+ L R +E
Sbjct: 649 TCSGPLLPRNVSTIFPPTTSSSDLDRRNKE 560
>BQ141665 similar to GP|18568267|gb putative polyprotein {Zea mays}, partial
(1%)
Length = 1195
Score = 26.2 bits (56), Expect = 7.8
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = +1
Query: 4 TTSHGSGRSGGGGSRRRARSRSPRRSR 30
T G+ +GGGG+RR S RR+R
Sbjct: 427 TRGAGNSPNGGGGARRPGASPGGRRAR 507
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.141 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,853,841
Number of Sequences: 36976
Number of extensions: 60663
Number of successful extensions: 578
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 536
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 555
length of query: 184
length of database: 9,014,727
effective HSP length: 90
effective length of query: 94
effective length of database: 5,686,887
effective search space: 534567378
effective search space used: 534567378
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 55 (25.8 bits)
Medicago: description of AC147364.11