BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147202.9 + phase: 0 /pseudo
(520 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-li... 154 1e-37
TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative ret... 80 7e-31
BG647708 weakly similar to GP|13786450|gb| putative reverse tran... 106 2e-23
BE248682 similar to GP|18568269|gb putative gag-pol polyprotein ... 65 5e-11
BG646730 52 7e-07
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 47 2e-05
AJ497561 weakly similar to GP|10140689|gb putative non-LTR retro... 44 2e-04
TC81772 42 4e-04
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 30 0.001
AW980456 39 0.006
BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At... 38 0.008
BG584442 38 0.008
BG585866 32 0.60
BE325721 similar to SP|P49147|PA2Y_ Cytosolic phospholipase A2 (... 28 6.6
>BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (18%)
Length = 789
Score = 154 bits (388), Expect = 1e-37
Identities = 81/187 (43%), Positives = 107/187 (56%)
Frame = -3
Query: 1 LILKVDMSKAYDRINWEYLRRVMLRLGFDAKWFGWIMMCVESVKYKVLVNQESEGPILPG 60
+ +K DM+KAYDRI W +LR V+ RLGF W WIM CV +V Y L+N +G +LP
Sbjct: 568 MAVKTDMTKAYDRIAWNFLREVLTRLGFHGIWISWIMECVSTVSYSFLINGGPQGRVLPS 389
Query: 61 SGLRKGDPLSPYLFILCAQGLTSLIDKAEGRGDLHGVRVCTGAPTFTHLLFVDDCFLFCR 120
GLR+GDPLSPYLFILC + L+ L +A +G L GV+V P HLLF DD F +
Sbjct: 388 RGLRQGDPLSPYLFILCTEVLSGLCQQALRKGTLPGVKVARNCPPINHLLFADDTMFFGK 209
Query: 121 AT*REVNALKNILSIYEDASGQQINFQKSEIFFSKNVGA*AKVNILNILQVNIAI*TGKY 180
+ L +I+ Y ASG+ IN KS I FS + L++ TGKY
Sbjct: 208 SNASSCAILLSIMDKYRAASGRCIN*TKSAITFSSKTSQAIIDRVKGELKIAKEGGTGKY 29
Query: 181 LGLPSVI 187
LG +++
Sbjct: 28 LGYRNIL 8
>TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative retroelement
{Oryza sativa (japonica cultivar-group)}, partial (1%)
Length = 625
Score = 80.1 bits (196), Expect(3) = 7e-31
Identities = 37/67 (55%), Positives = 48/67 (71%)
Frame = +2
Query: 38 MCVESVKYKVLVNQESEGPILPGSGLRKGDPLSPYLFILCAQGLTSLIDKAEGRGDLHGV 97
MCVES Y VLVN ++ PI+P GL++GD LSPY+FI+C +GL+ LI A+ RGD HG
Sbjct: 101 MCVESNDYYVLVNNDAVDPIIPSRGLQQGDHLSPYIFIICVEGLSFLIPHAKERGDTHGT 280
Query: 98 RVCTGAP 104
+ GAP
Sbjct: 281 SI*RGAP 301
Score = 55.1 bits (131), Expect(3) = 7e-31
Identities = 30/62 (48%), Positives = 42/62 (67%)
Frame = +3
Query: 129 LKNILSIYEDASGQQINFQKSEIFFSKNVGA*AKVNILNILQVNIAI*TGKYLGLPSVIG 188
+KNIL +YE+ SG+ I+ +KS I+ S+NV K +I IL V + T KYLGLPS+IG
Sbjct: 339 MKNILILYEEDSGKAISLRKS*IYCSRNVPDILKTSITYILGVQFMLGTCKYLGLPSMIG 518
Query: 189 TE 190
+
Sbjct: 519 RD 524
Score = 37.4 bits (85), Expect(3) = 7e-31
Identities = 11/30 (36%), Positives = 24/30 (79%)
Frame = +1
Query: 7 MSKAYDRINWEYLRRVMLRLGFDAKWFGWI 36
+SK Y+R++ +YL+ +M+++GF+ +W W+
Sbjct: 7 ISKVYNRVD*DYLKEIMIKMGFNNRWIYWM 96
>BG647708 weakly similar to GP|13786450|gb| putative reverse transcriptase
{Oryza sativa}, partial (9%)
Length = 708
Score = 106 bits (265), Expect = 2e-23
Identities = 50/99 (50%), Positives = 66/99 (66%)
Frame = +1
Query: 59 PGSGLRKGDPLSPYLFILCAQGLTSLIDKAEGRGDLHGVRVCTGAPTFTHLLFVDDCFLF 118
P GLR+GDPLSPYLFILCA L+ L+ + + +LHG++V P THLLF DD LF
Sbjct: 4 PEKGLRQGDPLSPYLFILCANVLSGLLKREGNKQNLHGIQVARSDPKITHLLFADDSLLF 183
Query: 119 CRAT*REVNALKNILSIYEDASGQQINFQKSEIFFSKNV 157
RA E + +L Y+ ASGQ +NF+KSE+ +S+NV
Sbjct: 184 ARANLTEAATIMQVLHSYQSASGQLVNFEKSEVSYSQNV 300
Score = 39.3 bits (90), Expect = 0.004
Identities = 20/60 (33%), Positives = 32/60 (53%)
Frame = +3
Query: 378 LNVKELLQPGQKSWNLDVLQHYLSSQSVFEAMNTLLFNSTAYDERVWRPGKRGIYTVRSA 437
L+V EL+ K WN D++ H ++ + + + L D+ +W K GIY+VRSA
Sbjct: 396 LSVDELIDYDTKQWNRDLIFHSFNNYAAHQIIKIPLSMRQPEDKIIWHWEKDGIYSVRSA 575
>BE248682 similar to GP|18568269|gb putative gag-pol polyprotein {Zea mays},
partial (1%)
Length = 441
Score = 65.5 bits (158), Expect = 5e-11
Identities = 34/90 (37%), Positives = 51/90 (55%)
Frame = +3
Query: 62 GLRKGDPLSPYLFILCAQGLTSLIDKAEGRGDLHGVRVCTGAPTFTHLLFVDDCFLFCRA 121
GL++GDPL+P+LF+L A+G++ L+ A R G V G +HL + DD
Sbjct: 27 GLKQGDPLAPFLFLLVAEGISGLMKNAVNRNLFQGFDVKRGGTRVSHLQYADDTLCIGMP 206
Query: 122 T*REVNALKNILSIYEDASGQQINFQKSEI 151
T + LK +L +E ASG ++NF KS +
Sbjct: 207 TVDNLWTLKALLQGFEMASGLKVNFHKSSL 296
>BG646730
Length = 799
Score = 51.6 bits (122), Expect = 7e-07
Identities = 34/125 (27%), Positives = 57/125 (45%), Gaps = 3/125 (2%)
Frame = +1
Query: 316 FEANKGNNLSYVWKSIHEAIPMVKQGTRWKIGNGDKIRVFQDRWLVNG---GLAQPAA*Y 372
F GN SY W+S+ ++ G+RW I NG +R+++D WL N + P +
Sbjct: 373 FALEIGNQPSYAWRSMFNIKDVIDLGSRWSISNGQNVRIWKDDWLPNQTGFKVCSPMVDF 552
Query: 373 NRLLQLNVKELLQPGQKSWNLDVLQHYLSSQSVFEAMNTLLFNSTAYDERVWRPGKRGIY 432
+ EL+ KS D++++ S+ + +N L D R+ + Y
Sbjct: 553 EE--ATCISELIDTNTKSSKRDLVRNVFSAFEAKQILNIPLSWRLWPDRRILYWERDRNY 726
Query: 433 TVRSA 437
+VRSA
Sbjct: 727 SVRSA 741
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 47.0 bits (110), Expect = 2e-05
Identities = 18/55 (32%), Positives = 31/55 (55%)
Frame = +3
Query: 2 ILKVDMSKAYDRINWEYLRRVMLRLGFDAKWFGWIMMCVESVKYKVLVNQESEGP 56
++K+D+ KAYD W +++ +ML LGF K+ W+M + + Y N + P
Sbjct: 450 MVKIDLXKAYDSXEWPFIKHLMLELGFPYKFVNWVMAXLTTASYTFNXNGDLTXP 614
>AJ497561 weakly similar to GP|10140689|gb putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 621
Score = 43.9 bits (102), Expect = 2e-04
Identities = 21/64 (32%), Positives = 34/64 (52%)
Frame = +2
Query: 305 LKARYYRRNAIFEANKGNNLSYVWKSIHEAIPMVKQGTRWKIGNGDKIRVFQDRWLVNGG 364
LK +Y+ + AN G+N S+ W+S+ ++ G RW IG+G +I V W+ N
Sbjct: 428 LKFKYFPQWDFSYANLGHNPSFTWRSLLSTQSLLTLGHRWMIGDGSQINVSSMSWIRNRP 607
Query: 365 LAQP 368
+P
Sbjct: 608 TLRP 619
>TC81772
Length = 982
Score = 42.4 bits (98), Expect = 4e-04
Identities = 16/40 (40%), Positives = 26/40 (65%)
Frame = -2
Query: 321 GNNLSYVWKSIHEAIPMVKQGTRWKIGNGDKIRVFQDRWL 360
G+N SY+W S+ ++++G WKIG+G I ++ D WL
Sbjct: 714 GHNPSYIWCSV*TLRMVLEEGHHWKIGDGSSINIWTDHWL 595
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 30.0 bits (66), Expect(2) = 0.001
Identities = 28/124 (22%), Positives = 44/124 (34%)
Frame = +2
Query: 26 LGFDAKWFGWIMMCVESVKYKVLVNQESEGPILPGSGLRKGDPLSPYLFILCAQGLTSLI 85
+ F W WI CV + VLVN ++ + LF + G+ + +
Sbjct: 380 MSFLVLWRKWIKECVSTATTSVLVNGSPTNVLMKSL-------VQTQLFTRYSFGVVNPV 538
Query: 86 DKAEGRGDLHGVRVCTGAPTFTHLLFVDDCFLFCRAT*REVNALKNILSIYEDASGQQIN 145
+HL F +D L + AL+ L I+ SG ++N
Sbjct: 539 -------------------VVSHLQFANDTLLLETKNWANIRALRAALVIF*AMSGLKVN 661
Query: 146 FQKS 149
F KS
Sbjct: 662 FHKS 673
Score = 30.0 bits (66), Expect(2) = 0.001
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +1
Query: 1 LILKVDMSKAYDRINWEYLRRVMLRLGF 28
L+ KVD KAY ++W YL V+ R F
Sbjct: 304 LLFKVDFEKAYHSVDWAYLDSVLGRYVF 387
>AW980456
Length = 779
Score = 38.5 bits (88), Expect = 0.006
Identities = 19/48 (39%), Positives = 27/48 (55%)
Frame = -2
Query: 409 MNTLLFNSTAYDERVWRPGKRGIYTVRSASRFCLTELVEGDYGCQFGN 456
M+T L + D +W+ K G Y V+SA RFC+ EL + Y + GN
Sbjct: 241 MSTPLISHA*LDRLIWKDEKHGKYYVKSAYRFCVEELFDSSYLHRPGN 98
>BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (10%)
Length = 767
Score = 38.1 bits (87), Expect = 0.008
Identities = 15/36 (41%), Positives = 24/36 (66%)
Frame = -3
Query: 321 GNNLSYVWKSIHEAIPMVKQGTRWKIGNGDKIRVFQ 356
G+ SY W+SIH A ++KQG + IGNG+ +++
Sbjct: 747 GSWASYAWRSIHSAQHLIKQGAKVIIGNGENTNIWE 640
>BG584442
Length = 775
Score = 38.1 bits (87), Expect = 0.008
Identities = 32/77 (41%), Positives = 43/77 (55%)
Frame = +2
Query: 210 **LNLYSNLFLPTL*VFF*FPHLLVMKSRK**ILYGREMVEIEIRESGGFHGTNYLSIKE 269
**L+++S +LP *VFF F L MK ++ *IL+ M+E +E G N L IK
Sbjct: 431 **LSMHSKAYLPM**VFFSF*ILK*MKLKRL*ILFHGFMLEKIAKECIGCLRRNCLYIKI 610
Query: 270 MVVWVLRIYICSIWHFL 286
M WVL+ SI+ L
Sbjct: 611 MEAWVLQTSRRSIFQCL 661
>BG585866
Length = 828
Score = 32.0 bits (71), Expect = 0.60
Identities = 29/118 (24%), Positives = 53/118 (44%), Gaps = 4/118 (3%)
Frame = +3
Query: 337 MVKQGTRWKIGNGDKIRVFQDRWLVNGGLAQPAA*YN-RLLQLNVKELLQPG-QKSWNLD 394
++K G W+ G+G+ + W G L A + L L VK++ G Q + +L
Sbjct: 102 VLKSGYTWRAGSGNS-SFWYTNWSSLGLLGTQAPFVDIHDLHLTVKDVFTTGGQHTQSL- 275
Query: 395 VLQHYLSSQSVFEAMNT--LLFNSTAYDERVWRPGKRGIYTVRSASRFCLTELVEGDY 450
+ + + E +N L FN++ D +W G+YT +S + L++ +Y
Sbjct: 276 ---YTILPTDIAEVINNTHLNFNASIGDAYIWPHNSNGVYTAKSGYSWILSQTETVNY 440
>BE325721 similar to SP|P49147|PA2Y_ Cytosolic phospholipase A2 (CPLA2)
[Includes: Phospholipase A2 (EC 3.1.1.4) (CPLA2),
partial (2%)
Length = 654
Score = 28.5 bits (62), Expect = 6.6
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = -2
Query: 15 NWEYLRRVMLRLGFDAKWFGWIMMCVESVKYKVLVNQESEGPIL 58
NW L RV LGF+ WFG+++ C E +++ E E +L
Sbjct: 134 NWVQLNRV---LGFEF-WFGFLLPCFEP---NIIIKSEREKKVL 24
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.344 0.154 0.514
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,282,519
Number of Sequences: 36976
Number of extensions: 231443
Number of successful extensions: 2042
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1500
Number of HSP's successfully gapped in prelim test: 52
Number of HSP's that attempted gapping in prelim test: 527
Number of HSP's gapped (non-prelim): 1574
length of query: 520
length of database: 9,014,727
effective HSP length: 100
effective length of query: 420
effective length of database: 5,317,127
effective search space: 2233193340
effective search space used: 2233193340
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC147202.9