BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147178.5 + phase: 0 /pseudo
(676 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AL368615 similar to SP|P09125|MSP8_ Merozoite surface protein CM... 264 7e-71
AL368616 weakly similar to GP|10177619|dbj contains similarity t... 127 1e-29
TC78356 weakly similar to GP|18092161|gb|AAL59140.1 cGMP-gated c... 35 0.094
TC85985 similar to GP|5139695|dbj|BAA81686.1 expressed in cucumb... 35 0.12
TC89570 similar to GP|13936308|gb|AAK40307.1 putative methyl-bin... 34 0.16
TC85625 SP|Q40345|IDHP_MEDSA Isocitrate dehydrogenase [NADP] ch... 34 0.21
CB894755 similar to GP|9757890|db phosphoribosylanthranilate tra... 33 0.27
TC87847 similar to GP|15929245|gb|AAH15068.1 Similar to adaptor-... 32 0.61
TC88911 weakly similar to GP|13926280|gb|AAK49610.1 AT5g14920/F2... 32 0.79
TC83787 similar to GP|9757890|dbj|BAB08397.1 phosphoribosylanthr... 32 1.0
TC77641 similar to GP|21595125|gb|AAM66074.1 endosperm-specific ... 31 1.4
TC87729 similar to GP|9759461|dbj|BAB10377.1 gene_id:MAF19.15~un... 31 1.8
BQ140761 weakly similar to GP|18076245|emb phosphophoryn {Rattus... 30 2.3
BQ155388 similar to GP|20197493|gb|A expressed protein {Arabidop... 30 3.9
BF646634 weakly similar to GP|160687|gb|AA serine rich protein (... 29 6.7
TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 29 6.7
TC77658 similar to PIR|T46895|T46895 acyl-CoA dehydrogenase (EC ... 29 6.7
TC79086 similar to GP|23499033|emb|CAD51113. ubiquitin-protein l... 29 6.7
TC89826 weakly similar to PIR|T49138|T49138 hypothetical protein... 28 8.8
TC87583 similar to GP|19715579|gb|AAL91615.1 AT3g24740/K7P8_3 {A... 28 8.8
>AL368615 similar to SP|P09125|MSP8_ Merozoite surface protein CMZ-8
(Fragment). {Eimeria acervulina}, partial (5%)
Length = 498
Score = 264 bits (675), Expect = 7e-71
Identities = 131/131 (100%), Positives = 131/131 (100%)
Frame = +1
Query: 1 MDFDDLDAPVKPRVSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPV 60
MDFDDLDAPVKPRVSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPV
Sbjct: 106 MDFDDLDAPVKPRVSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPV 285
Query: 61 KHEPNASVKTDAESKIEGKVDSRNVDVEMTEAVDGSREVNPMDEEDTVVREIDVYFSPSI 120
KHEPNASVKTDAESKIEGKVDSRNVDVEMTEAVDGSREVNPMDEEDTVVREIDVYFSPSI
Sbjct: 286 KHEPNASVKTDAESKIEGKVDSRNVDVEMTEAVDGSREVNPMDEEDTVVREIDVYFSPSI 465
Query: 121 DNDTKLYVMQY 131
DNDTKLYVMQY
Sbjct: 466 DNDTKLYVMQY 498
>AL368616 weakly similar to GP|10177619|dbj contains similarity to unknown
protein~dbj|BAA91655.1~gene_id:K6M13.8 {Arabidopsis
thaliana}, partial (5%)
Length = 538
Score = 127 bits (320), Expect = 1e-29
Identities = 61/61 (100%), Positives = 61/61 (100%)
Frame = +1
Query: 611 EVACDIHGCYVLKSAQNDPFRDVVIDMLRGSGPNGKLKKAEILEAARRKLGRDVPNNEYI 670
EVACDIHGCYVLKSAQNDPFRDVVIDMLRGSGPNGKLKKAEILEAARRKLGRDVPNNEYI
Sbjct: 1 EVACDIHGCYVLKSAQNDPFRDVVIDMLRGSGPNGKLKKAEILEAARRKLGRDVPNNEYI 180
Query: 671 K 671
K
Sbjct: 181 K 183
>TC78356 weakly similar to GP|18092161|gb|AAL59140.1 cGMP-gated channel
{Oncorhynchus mykiss}, partial (4%)
Length = 854
Score = 35.0 bits (79), Expect = 0.094
Identities = 27/83 (32%), Positives = 38/83 (45%), Gaps = 7/83 (8%)
Frame = +1
Query: 30 EPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNASVKT-------DAESKIEGKVDS 82
EP VP+ P P+ TE ++ T TPV E N S+ T +A+ EGK D+
Sbjct: 313 EPVSVPEPAPVPI----TEEVKVGATEETPV--EDNKSLNTLLNENVEEAKKPEEGKADA 474
Query: 83 RNVDVEMTEAVDGSREVNPMDEE 105
V E E + EV +E+
Sbjct: 475 EEVKAEPKEEEPKTEEVKGQEEK 543
>TC85985 similar to GP|5139695|dbj|BAA81686.1 expressed in cucumber
hypocotyls {Cucumis sativus}, partial (42%)
Length = 892
Score = 34.7 bits (78), Expect = 0.12
Identities = 20/51 (39%), Positives = 25/51 (48%), Gaps = 1/51 (1%)
Frame = +3
Query: 18 APKSSKFKPKKEEPSLVPKSEPPPLA-SAKTEPQEIDLTAPTPVKHEPNAS 67
AP ++ KPK P PKS PP + +A T + AP PV P AS
Sbjct: 177 APVAAPTKPKSPAPVASPKSSPPASSPTAATVTPAVSPAAPVPVAKSPAAS 329
>TC89570 similar to GP|13936308|gb|AAK40307.1 putative methyl-binding domain
protein MBD105 {Zea mays}, partial (9%)
Length = 728
Score = 34.3 bits (77), Expect = 0.16
Identities = 25/93 (26%), Positives = 40/93 (42%), Gaps = 6/93 (6%)
Frame = +3
Query: 20 KSSKFKPK-KEEPSLVPKSEPPPLAS-----AKTEPQEIDLTAPTPVKHEPNASVKTDAE 73
+SS+ K K P PK++PP S +K E E + V+ + K D +
Sbjct: 342 RSSRIVEKVKAAPPAEPKTDPPKKRSRKSSASKKEASEDEAEETKDVEMQEAGETKDDKD 521
Query: 74 SKIEGKVDSRNVDVEMTEAVDGSREVNPMDEED 106
++E KV + N D + E D + P + D
Sbjct: 522 VELEKKVVNENEDKKGAEDADVKESIQPGETTD 620
>TC85625 SP|Q40345|IDHP_MEDSA Isocitrate dehydrogenase [NADP] chloroplast
precursor (EC 1.1.1.42) (Oxalosuccinate decarboxylase),
partial (97%)
Length = 1733
Score = 33.9 bits (76), Expect = 0.21
Identities = 26/83 (31%), Positives = 38/83 (45%), Gaps = 4/83 (4%)
Frame = -2
Query: 234 KNVATSNNQNKQTTPSMEQKSDGAPSIEQKSDSNQCWVPLEYHGCKS--DISSRYFQQM- 290
K + SNN K T + PSI Q C+VPL Y KS + SR F +
Sbjct: 1510 KQIKQSNNMGKNPTLAF-------PSIRQMKQK*PCFVPLSYFF*KSI*SLQSRPFSHLP 1352
Query: 291 -VTHESSPINFEMSTYDYIATLC 312
+T+ S + F++S++ LC
Sbjct: 1351 KITNLSRDLCFKISSHSIDEFLC 1283
>CB894755 similar to GP|9757890|db phosphoribosylanthranilate
transferase-like protein {Arabidopsis thaliana}, partial
(10%)
Length = 674
Score = 33.5 bits (75), Expect = 0.27
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 1/65 (1%)
Frame = +3
Query: 6 LDAPVKPRVSRFAPKSSKFKPKKEEPSL-VPKSEPPPLASAKTEPQEIDLTAPTPVKHEP 64
+D + PR S P++ P E PS+ V +S PPP K EP P P E
Sbjct: 342 VDEEIPPRPSAPVPENPP-TPSSEPPSVKVEESPPPPSEPEKVEPPPPSEAGPQPKAEEV 518
Query: 65 NASVK 69
N +++
Sbjct: 519 NKNLR 533
>TC87847 similar to GP|15929245|gb|AAH15068.1 Similar to adaptor-related
protein complex AP-3 beta 1 subunit {Mus musculus},
partial (2%)
Length = 931
Score = 32.3 bits (72), Expect = 0.61
Identities = 27/86 (31%), Positives = 40/86 (46%), Gaps = 2/86 (2%)
Frame = +1
Query: 28 KEEPSLVPKSEPPPLAS--AKTEPQEIDLTAPTPVKHEPNASVKTDAESKIEGKVDSRNV 85
K EP+ P + PLAS KT+ Q + P P+K + K AES DS+
Sbjct: 412 KSEPTPPPNPKVKPLASKPMKTQTQAQAQSTPVPIK----SGTKRVAESSTGN--DSKRS 573
Query: 86 DVEMTEAVDGSREVNPMDEEDTVVRE 111
+ T A GS + N ++E+ + E
Sbjct: 574 KKKTTTAGGGSDDENEVEEDAKLTGE 651
>TC88911 weakly similar to GP|13926280|gb|AAK49610.1 AT5g14920/F2G14_40
{Arabidopsis thaliana}, partial (11%)
Length = 1320
Score = 32.0 bits (71), Expect = 0.79
Identities = 27/68 (39%), Positives = 33/68 (47%), Gaps = 5/68 (7%)
Frame = +2
Query: 8 APVKPRVSRF-APKSSKFKPKKEEPSLVPKSEPPPLASAKT----EPQEIDLTAPTPVKH 62
AP+KP V + APK S KP VPKS+PP A K+ P+ + P P K
Sbjct: 257 APLKPPVPKAPAPKPSPVKPP------VPKSKPPTTAPVKSPVPDPPKLAPVKLPVP-KV 415
Query: 63 EPNASVKT 70
P S KT
Sbjct: 416 TPALSPKT 439
>TC83787 similar to GP|9757890|dbj|BAB08397.1 phosphoribosylanthranilate
transferase-like protein {Arabidopsis thaliana}, partial
(11%)
Length = 1008
Score = 31.6 bits (70), Expect = 1.0
Identities = 24/93 (25%), Positives = 34/93 (35%)
Frame = +2
Query: 9 PVKPRVSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNASV 68
P P P S K + PS + K EPPP P P P + P
Sbjct: 194 PENPPAPSSEPPSGKVEESPPPPSELEKVEPPPSDQPSGAP-------PPPSEAGPQPKA 352
Query: 69 KTDAESKIEGKVDSRNVDVEMTEAVDGSREVNP 101
+ + E K+E + + V+ E V + NP
Sbjct: 353 EGEQEPKVEPEPVPEQIPVQ--EEVVDPMDANP 445
>TC77641 similar to GP|21595125|gb|AAM66074.1 endosperm-specific
protein-like protein {Arabidopsis thaliana}, partial
(82%)
Length = 1488
Score = 31.2 bits (69), Expect = 1.4
Identities = 21/62 (33%), Positives = 28/62 (44%), Gaps = 5/62 (8%)
Frame = +3
Query: 14 VSRFAPKSSKFKPKKEE--PSLVPKSEPPPLASAKTE---PQEIDLTAPTPVKHEPNASV 68
V+ +P SS P + P+ PKS PP L + T P+ T TP K P+ S
Sbjct: 27 VTTLSPSSSSSSPSPPQSPPTTSPKSSPPTLTTPNTTTSFPKPNSPTKSTPAKPSPS*SS 206
Query: 69 KT 70
T
Sbjct: 207TT 212
>TC87729 similar to GP|9759461|dbj|BAB10377.1 gene_id:MAF19.15~unknown
protein {Arabidopsis thaliana}, partial (37%)
Length = 2210
Score = 30.8 bits (68), Expect = 1.8
Identities = 48/199 (24%), Positives = 70/199 (35%), Gaps = 15/199 (7%)
Frame = +1
Query: 81 DSRNVDVEMTEAVDGSREVNPMDEEDTV-----VREIDVYFSPSIDNDTKL-YVMQYPLR 134
D + VD ++ GS P D++D EI F D + + Y Q +
Sbjct: 427 DEQEVDAAARRSISGS----PRDDKDHTHDLDSAPEIRDVFGDFDDEEEDMGYAAQQDIE 594
Query: 135 PSWRPYDLDEQCE-EVRLKPGTSEVEVDLSIDLESYNVEKATADKLKYTKQTLSTTWKPP 193
Y +E+ E L+P + + + E N+E T DK L +PP
Sbjct: 595 QDSNRYPAEEEGSYEKSLRPEDILADEEHQYESEEENIEMKTKDKPLGPPLELEIPLRPP 774
Query: 194 PA-----NGCAVGLLMGDKLHLHPIHAVVQLRPSRHYLDSGGSEKKNVATSNN---QNKQ 245
PA N V +MG + P D G+ KK + NN K
Sbjct: 775 PALPGKMNVFKVSNIMG--VDPKPFDPKTYEEEDTFVTDEFGN-KKRIRLENNIVRWRKV 945
Query: 246 TTPSMEQKSDGAPSIEQKS 264
T P DG S+E +
Sbjct: 946 TNP------DGTTSVESNA 984
>BQ140761 weakly similar to GP|18076245|emb phosphophoryn {Rattus
norvegicus}, partial (9%)
Length = 620
Score = 30.4 bits (67), Expect = 2.3
Identities = 21/81 (25%), Positives = 38/81 (45%)
Frame = +1
Query: 18 APKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNASVKTDAESKIE 77
APK K K K+ S+ S+ +S+++E + P P + + ++S +D++S +
Sbjct: 379 APKKEKAKKAKKAKSVSSSSDSSDDSSSESESEPEPXNVPLP-ESDDSSSASSDSDSSSD 555
Query: 78 GKVDSRNVDVEMTEAVDGSRE 98
DS + D D S E
Sbjct: 556 SSSDSSS-DSSSXSDXDSSSE 615
>BQ155388 similar to GP|20197493|gb|A expressed protein {Arabidopsis
thaliana}, partial (49%)
Length = 498
Score = 29.6 bits (65), Expect = 3.9
Identities = 13/43 (30%), Positives = 25/43 (57%)
Frame = +2
Query: 14 VSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTA 56
+SR +++ + PS +P+ PPPLA+ +T+P+ + A
Sbjct: 32 LSRSFISTARSSLRPAAPSPLPRLRPPPLATPRTQPRLFSMPA 160
>BF646634 weakly similar to GP|160687|gb|AA serine rich protein (SERP I)
{Plasmodium falciparum}, partial (3%)
Length = 616
Score = 28.9 bits (63), Expect = 6.7
Identities = 19/38 (50%), Positives = 25/38 (65%)
Frame = +1
Query: 69 KTDAESKIEGKVDSRNVDVEMTEAVDGSREVNPMDEED 106
K+ +SK + KV S N++VEM EA GS +V DEED
Sbjct: 277 KSGVKSKKKKKV-SFNLNVEMYEANPGSYQVLDDDEED 387
>TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (13%)
Length = 663
Score = 28.9 bits (63), Expect = 6.7
Identities = 23/100 (23%), Positives = 40/100 (40%)
Frame = +3
Query: 7 DAPVKPRVSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNA 66
D K + + K K K +E+ S K + K E + K++
Sbjct: 324 DKEKKKKEKKEKGKEDKDKDGEEKKSKKDKEKKKD----KNEDDDEGEDGSKKKKNKDKK 491
Query: 67 SVKTDAESKIEGKVDSRNVDVEMTEAVDGSREVNPMDEED 106
K + + K EGKV R++D+E T ++ D+E+
Sbjct: 492 EKKKEEDEKEEGKVSVRDIDIEETAKEGKEKKKKKEDKEE 611
>TC77658 similar to PIR|T46895|T46895 acyl-CoA dehydrogenase (EC 1.3.99.3)
[validated] - Arabidopsis thaliana, partial (94%)
Length = 2886
Score = 28.9 bits (63), Expect = 6.7
Identities = 20/58 (34%), Positives = 30/58 (51%)
Frame = -3
Query: 165 DLESYNVEKATADKLKYTKQTLSTTWKPPPANGCAVGLLMGDKLHLHPIHAVVQLRPS 222
DL Y++ + ++ Y LST + P +N + LL K HLH H+ VQ RP+
Sbjct: 805 DLLRYSLRR*PTNQQMYC--FLSTAFGPQGSNHLPL*LLWSSK-HLHHFHSQVQSRPN 641
>TC79086 similar to GP|23499033|emb|CAD51113. ubiquitin-protein ligase 1
putative {Plasmodium falciparum 3D7}, partial (0%)
Length = 1418
Score = 28.9 bits (63), Expect = 6.7
Identities = 28/124 (22%), Positives = 50/124 (39%)
Frame = +2
Query: 7 DAPVKPRVSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNA 66
+AP + PK++ + + EE + + EPPP KT+P E L + T P
Sbjct: 200 EAPKTTPPPKTNPKNNSVEDEDEEETDTDEDEPPP----KTKPIEQTLKSGTKPSIAP-- 361
Query: 67 SVKTDAESKIEGKVDSRNVDVEMTEAVDGSREVNPMDEEDTVVREIDVYFSPSIDNDTKL 126
++ + E ++ + TE + +E P EED F D ++
Sbjct: 362 -ARSGTKRPAENNDTKQSNKKKTTEEKNKKKEKEP--EEDDNSNNKKASFQRVFTEDDEV 532
Query: 127 YVMQ 130
++Q
Sbjct: 533 AILQ 544
>TC89826 weakly similar to PIR|T49138|T49138 hypothetical protein T10D17.10
- Arabidopsis thaliana, partial (59%)
Length = 1130
Score = 28.5 bits (62), Expect = 8.8
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +3
Query: 38 EPPPLASAKTEPQEIDLTAPTPVKHEP 64
+P P++S+KT+P T+P P KH P
Sbjct: 213 QPNPVSSSKTQPSSPS-TSPPPEKHHP 290
>TC87583 similar to GP|19715579|gb|AAL91615.1 AT3g24740/K7P8_3 {Arabidopsis
thaliana}, partial (41%)
Length = 1220
Score = 28.5 bits (62), Expect = 8.8
Identities = 21/87 (24%), Positives = 38/87 (43%)
Frame = -3
Query: 201 GLLMGDKLHLHPIHAVVQLRPSRHYLDSGGSEKKNVATSNNQNKQTTPSMEQKSDGAPSI 260
G +G ++HL P+H + HYL+ +N+ K + Q +D A +I
Sbjct: 780 GAFLGHEMHLQPVH*IFVNLLRLHYLED---------PTNSHPKDSDDG--QMNDDALTI 634
Query: 261 EQKSDSNQCWVPLEYHGCKSDISSRYF 287
K N+ VP ++ C +R++
Sbjct: 633 HLK---NELLVP*QFQWCYPSFGTRWW 562
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.327 0.140 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,998,807
Number of Sequences: 36976
Number of extensions: 304568
Number of successful extensions: 2297
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 2208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2284
length of query: 676
length of database: 9,014,727
effective HSP length: 103
effective length of query: 573
effective length of database: 5,206,199
effective search space: 2983152027
effective search space used: 2983152027
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC147178.5