BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147178.4 - phase: 0
(248 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87684 similar to PIR|E85184|E85184 hypothetical protein dl4315... 414 e-132
TC79911 similar to PIR|E85184|E85184 hypothetical protein dl4315... 275 1e-74
AW776618 homologue to GP|13359435|db putative senescence-associa... 118 2e-27
TC82616 weakly similar to GP|19310556|gb|AAL85011.1 putative pro... 34 0.060
TC78226 similar to GP|20873469|emb|CAD12839. putative metallopho... 30 0.66
TC88803 similar to GP|20466193|gb|AAM20414.1 unknown protein {Ar... 30 0.86
TC81075 GP|2688966|gb|AAB88887.1| carnitine acetyl transferase {... 29 1.5
TC86518 homologue to PIR|T09640|T09640 protein phosphatase 2C - ... 27 7.3
TC79574 similar to PIR|T05688|T05688 hypothetical protein F20M13... 27 7.3
AL382068 homologue to GP|9279716|dbj ABC transporter {Arabidopsi... 27 7.3
>TC87684 similar to PIR|E85184|E85184 hypothetical protein dl4315c [imported]
- Arabidopsis thaliana, partial (65%)
Length = 1700
Score = 414 bits (1063), Expect(2) = e-132
Identities = 209/213 (98%), Positives = 209/213 (98%)
Frame = +2
Query: 1 MLSGSCYLPHPDKVATGGEDAHFICEDEQAIGVADGVGGWADVGVNAGLYAQELVANSAR 60
MLSGSCYLPHPDKVATGGEDAHFICEDEQAIGVADGVGGWADVGVNA LYAQELVAN AR
Sbjct: 863 MLSGSCYLPHPDKVATGGEDAHFICEDEQAIGVADGVGGWADVGVNARLYAQELVANCAR 1042
Query: 61 AIREEPKGSFNPVRVLEKAHSKTKAMGSSTVCIIALIDEALNAINLGDSGFIVIRDGSVI 120
A EEPKGSFNPVRVLEKAHSKTKAMGSSTVCIIALIDEALNAINLGDSGFIVIRDGSVI
Sbjct: 1043 ASSEEPKGSFNPVRVLEKAHSKTKAMGSSTVCIIALIDEALNAINLGDSGFIVIRDGSVI 1222
Query: 121 FKSPVQQRGFNFPYQLARSGTEGDLPSSGEVFTVPVAPGDIIVAGTDGLFDNMYNNDIVG 180
FKSPVQQRGFNFPYQLARSGTEGDLPSSGEVFTVPVAPGDIIVAGTDGLFDNMYNNDIVG
Sbjct: 1223 FKSPVQQRGFNFPYQLARSGTEGDLPSSGEVFTVPVAPGDIIVAGTDGLFDNMYNNDIVG 1402
Query: 181 VVVGATRARLGPQATAQKIAALARQRALDTKRQ 213
VVVGATRARLGPQATAQKIAALARQRALDTKRQ
Sbjct: 1403 VVVGATRARLGPQATAQKIAALARQRALDTKRQ 1501
Score = 75.1 bits (183), Expect(2) = e-132
Identities = 37/41 (90%), Positives = 38/41 (92%)
Frame = +3
Query: 208 LDTKRQSPFSAAALEYGYRFDGGKLDDLTVVVSYISNSVNE 248
L K +SPFSAAALEYGYRFDGGKLDDLTVVVSYISNSVNE
Sbjct: 1485 LTRKGRSPFSAAALEYGYRFDGGKLDDLTVVVSYISNSVNE 1607
>TC79911 similar to PIR|E85184|E85184 hypothetical protein dl4315c
[imported] - Arabidopsis thaliana, partial (57%)
Length = 924
Score = 275 bits (703), Expect = 1e-74
Identities = 142/201 (70%), Positives = 163/201 (80%)
Frame = +3
Query: 45 VNAGLYAQELVANSARAIREEPKGSFNPVRVLEKAHSKTKAMGSSTVCIIALIDEALNAI 104
VN+G Y++EL++NS AIREEPKGS +P RVLEKA+S TKA GSST CIIAL D+ L+AI
Sbjct: 12 VNSGYYSRELMSNSVDAIREEPKGSVDPARVLEKAYSSTKAKGSSTACIIALTDQGLHAI 191
Query: 105 NLGDSGFIVIRDGSVIFKSPVQQRGFNFPYQLARSGTEGDLPSSGEVFTVPVAPGDIIVA 164
NLGDSGF+VIRDG IF+SPVQQ FNF YQL S + DLPSSG+VFTV VAPGD+IVA
Sbjct: 192 NLGDSGFMVIRDGCTIFQSPVQQHDFNFTYQLECS-SHSDLPSSGQVFTVAVAPGDVIVA 368
Query: 165 GTDGLFDNMYNNDIVGVVVGATRARLGPQATAQKIAALARQRALDTKRQSPFSAAALEYG 224
GTDGLFDN+YNN+I VVV RA PQ TAQKIAALARQRALD RQ+PFS AA + G
Sbjct: 369 GTDGLFDNLYNNEITAVVVHGVRAGFSPQVTAQKIAALARQRALDKDRQTPFSTAAQDAG 548
Query: 225 YRFDGGKLDDLTVVVSYISNS 245
+R+ GGKLDD TVVVSYI+ S
Sbjct: 549 FRYYGGKLDDTTVVVSYITGS 611
>AW776618 homologue to GP|13359435|db putative senescence-associated protein
{Pisum sativum}, partial (56%)
Length = 613
Score = 118 bits (296), Expect = 2e-27
Identities = 78/207 (37%), Positives = 117/207 (55%), Gaps = 3/207 (1%)
Frame = +3
Query: 17 GGEDAHFICE-DEQAIGVADGVGGWADVGVNAGLYAQELVANSARAIREEPKGSFNPVRV 75
GGEDA F+ + I VADGV GWA+ V L+ +EL+AN+ +++E + +P +
Sbjct: 6 GGEDAFFVSNYNGGVIAVADGVSGWAEEDVYPSLFPRELMANAYNFVQDEEVNN-DPQIL 182
Query: 76 LEKAHSKTKAMGSSTVCIIALIDEA--LNAINLGDSGFIVIRDGSVIFKSPVQQRGFNFP 133
+ KAH+ T + GS+TV I+A++++ L N+GD G VIR+G VIF + Q+ F+ P
Sbjct: 183 IRKAHAATFSTGSATV-IVAMLEKNGNLKIANVGDCGLRVIRNGQVIFSTSPQEHYFDCP 359
Query: 134 YQLARSGTEGDLPSSGEVFTVPVAPGDIIVAGTDGLFDNMYNNDIVGVVVGATRARLGPQ 193
YQL+ S G V V + GD IV G+DGLFDN+++++I T A
Sbjct: 360 YQLS-SERVGQTYLDAMVSNVELMEGDTIVMGSDGLFDNVFDHEI-----ALTVANKEVS 521
Query: 194 ATAQKIAALARQRALDTKRQSPFSAAA 220
AQ + LA ++D SP+S A
Sbjct: 522 EAAQALTNLANSHSMDLNFDSPYSLEA 602
>TC82616 weakly similar to GP|19310556|gb|AAL85011.1 putative protein
phosphatase-2C {Arabidopsis thaliana}, partial (35%)
Length = 1184
Score = 33.9 bits (76), Expect = 0.060
Identities = 19/85 (22%), Positives = 39/85 (45%)
Frame = +2
Query: 160 DIIVAGTDGLFDNMYNNDIVGVVVGATRARLGPQATAQKIAALARQRALDTKRQSPFSAA 219
+ +V +DG++D + NN+++ +V A R ++TA K+ A
Sbjct: 479 EFVVLASDGIWDVLTNNEVINIVASAPR-----KSTAAKMLV---------------KRA 598
Query: 220 ALEYGYRFDGGKLDDLTVVVSYISN 244
+ Y++ G K+DD V ++ +
Sbjct: 599 VKAWMYKYPGSKIDDCAAVCLFLDD 673
>TC78226 similar to GP|20873469|emb|CAD12839. putative metallophosphatase
{Lupinus luteus}, partial (58%)
Length = 1150
Score = 30.4 bits (67), Expect = 0.66
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = +3
Query: 121 FKSPVQQRGFNFPYQLARSGTEGDLPSSGEVFTVPVAPGD 160
F + VQQ PY +A E D P+SG + P + G+
Sbjct: 267 FTAQVQQISSKVPYMIASGNHERDWPNSGSFYDTPDSGGE 386
>TC88803 similar to GP|20466193|gb|AAM20414.1 unknown protein {Arabidopsis
thaliana}, partial (61%)
Length = 1247
Score = 30.0 bits (66), Expect = 0.86
Identities = 16/63 (25%), Positives = 29/63 (45%)
Frame = +3
Query: 97 IDEALNAINLGDSGFIVIRDGSVIFKSPVQQRGFNFPYQLARSGTEGDLPSSGEVFTVPV 156
+DE + +NLG +G++ R G + F S Y EG + + G+++ V
Sbjct: 93 VDEEIGGLNLGSAGYLGRRQGVINFTS---------TYDSRTQEVEGSIATRGDLWRVEA 245
Query: 157 APG 159
+ G
Sbjct: 246 SRG 254
>TC81075 GP|2688966|gb|AAB88887.1| carnitine acetyl transferase {Magnaporthe
grisea}, partial (3%)
Length = 351
Score = 29.3 bits (64), Expect = 1.5
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = -2
Query: 173 MYNNDIVGVVVGATRARLGPQATAQKIAALARQRALDT 210
+YN +VG+ VG R+R P A+ +K L R LDT
Sbjct: 200 LYNLALVGLRVGIRRSRKSPAASFRKKLILWLPRLLDT 87
>TC86518 homologue to PIR|T09640|T09640 protein phosphatase 2C - alfalfa,
partial (50%)
Length = 831
Score = 26.9 bits (58), Expect = 7.3
Identities = 19/87 (21%), Positives = 33/87 (37%)
Frame = +1
Query: 160 DIIVAGTDGLFDNMYNNDIVGVVVGATRARLGPQATAQKIAALARQRALDTKRQSPFSAA 219
D+++ +DGL+D + N + V + ARQ + Q P A
Sbjct: 385 DLLILASDGLWDKVSNQEAVDI---------------------ARQFCVGNNNQQPLMAC 501
Query: 220 ALEYGYRFDGGKLDDLTVVVSYISNSV 246
G LDD +V++ + + V
Sbjct: 502 KKLAKLSVSRGSLDDTSVMIIKLKHYV 582
>TC79574 similar to PIR|T05688|T05688 hypothetical protein F20M13.160 -
Arabidopsis thaliana, partial (38%)
Length = 1412
Score = 26.9 bits (58), Expect = 7.3
Identities = 14/36 (38%), Positives = 21/36 (57%), Gaps = 1/36 (2%)
Frame = +2
Query: 152 FTVPVAPGDIIVAGTDGLFDNMYN-NDIVGVVVGAT 186
FT+P P ++ +G+D NM N D V ++V AT
Sbjct: 491 FTLPGYPDIVLASGSDHTMVNMRNLEDYVSLIVDAT 598
>AL382068 homologue to GP|9279716|dbj ABC transporter {Arabidopsis thaliana},
partial (6%)
Length = 274
Score = 26.9 bits (58), Expect = 7.3
Identities = 19/82 (23%), Positives = 36/82 (43%), Gaps = 2/82 (2%)
Frame = +3
Query: 51 AQELVANSARAIREEPKGSFNP--VRVLEKAHSKTKAMGSSTVCIIALIDEALNAINLGD 108
A ELVAN + +EP + ++ + T G + VC I + +++ D
Sbjct: 18 AVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVDTGRTVVCTIH--EPSIDIFEAFD 191
Query: 109 SGFIVIRDGSVIFKSPVQQRGF 130
++ R G VI+ P+ + +
Sbjct: 192 ELLLMKRGGQVIYAGPLGRHSY 257
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.136 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,853,202
Number of Sequences: 36976
Number of extensions: 61659
Number of successful extensions: 250
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 247
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 247
length of query: 248
length of database: 9,014,727
effective HSP length: 94
effective length of query: 154
effective length of database: 5,538,983
effective search space: 853003382
effective search space used: 853003382
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC147178.4