Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC147178.12 + phase: 0 
         (163 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC90609 similar to GP|21555067|gb|AAM63768.1 unknown {Arabidopsi...   334  1e-92
BQ751450 similar to GP|21954534|db 6-phosphogluconate dehydrogen...    28  1.2
TC89624 similar to GP|20160656|dbj|BAB89601. contains EST AU0581...    28  1.6
TC78518 similar to PIR|JC7234|JC7234 27k vesicle-associated memb...    28  1.6
TC82617 similar to GP|14190477|gb|AAK55719.1 AT5g64260/MSJ1_10 {...    28  2.1
AA660656 weakly similar to GP|6498428|dbj| Similar to Arabidopsi...    27  2.8
BQ150300 similar to GP|10178184|dbj 50S ribosomal protein L29 {A...    27  3.6
BF633702                                                               26  6.2
TC78434 similar to GP|3790100|gb|AAC67586.1| pyrophosphate-depen...    26  8.1
TC91118                                                                26  8.1
TC85214 similar to PIR|S26688|S26688 legumin K - garden pea, par...    26  8.1
TC81332 similar to GP|15810167|gb|AAL06985.1 At1g64110/F22C12_22...    26  8.1

>TC90609 similar to GP|21555067|gb|AAM63768.1 unknown {Arabidopsis
           thaliana}, partial (71%)
          Length = 661

 Score =  334 bits (856), Expect = 1e-92
 Identities = 163/163 (100%), Positives = 163/163 (100%)
 Frame = +3

Query: 1   MLLFSPISTPKTFTNHLNHPLRKPKSHHVRLKLKITNMAKEGSDTNSSPTEIAAIAGGLI 60
           MLLFSPISTPKTFTNHLNHPLRKPKSHHVRLKLKITNMAKEGSDTNSSPTEIAAIAGGLI
Sbjct: 45  MLLFSPISTPKTFTNHLNHPLRKPKSHHVRLKLKITNMAKEGSDTNSSPTEIAAIAGGLI 224

Query: 61  STPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVSYLVVVGIVAWSIYTKTKTGSGLPNGP 120
           STPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVSYLVVVGIVAWSIYTKTKTGSGLPNGP
Sbjct: 225 STPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVSYLVVVGIVAWSIYTKTKTGSGLPNGP 404

Query: 121 FGLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPADQCFG 163
           FGLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPADQCFG
Sbjct: 405 FGLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPADQCFG 533


>BQ751450 similar to GP|21954534|db 6-phosphogluconate dehydrogenase
           {Aspergillus oryzae}, partial (36%)
          Length = 661

 Score = 28.5 bits (62), Expect = 1.2
 Identities = 11/18 (61%), Positives = 14/18 (77%)
 Frame = -2

Query: 17  LNHPLRKPKSHHVRLKLK 34
           L+HPLR P+ HH RL L+
Sbjct: 486 LHHPLRLPQGHHQRLPLQ 433


>TC89624 similar to GP|20160656|dbj|BAB89601. contains EST
           AU058101(S2419)~similar to Arabidopsis thaliana
           chromosome5 At5g58640~unknown protein, partial (60%)
          Length = 730

 Score = 28.1 bits (61), Expect = 1.6
 Identities = 16/56 (28%), Positives = 25/56 (44%)
 Frame = +2

Query: 3   LFSPISTPKTFTNHLNHPLRKPKSHHVRLKLKITNMAKEGSDTNSSPTEIAAIAGG 58
           LFSP   PK   ++  HP+ +P+  H++  L      + G D    P  +  I  G
Sbjct: 125 LFSPSPPPKPSRHNHVHPIPQPQQSHLQQPLDFPIQKQSGID----PIGVGPIGAG 280


>TC78518 similar to PIR|JC7234|JC7234 27k vesicle-associated membrane
           protein-associated protein - curled-leaved tobacco,
           partial (83%)
          Length = 827

 Score = 28.1 bits (61), Expect = 1.6
 Identities = 21/62 (33%), Positives = 33/62 (52%), Gaps = 10/62 (16%)
 Frame = +1

Query: 2   LLFSPISTPKTFTNHLNHPL-RKPKSHHVR-LKLKITNMA--------KEGSDTNSSPTE 51
           LL S I++P T T  +N  +  K   H+V   KL++  +A        +EGSD +SSP  
Sbjct: 343 LLQSVIASPGTTTKDINPEMFSKEAGHNVEECKLRVVYVAPPGPPSPVREGSDEDSSPDS 522

Query: 52  IA 53
           ++
Sbjct: 523 VS 528


>TC82617 similar to GP|14190477|gb|AAK55719.1 AT5g64260/MSJ1_10 {Arabidopsis
           thaliana}, partial (47%)
          Length = 710

 Score = 27.7 bits (60), Expect = 2.1
 Identities = 11/29 (37%), Positives = 15/29 (50%)
 Frame = -1

Query: 60  ISTPVIGWSLYTLKTTGCGLPPGPGGSIG 88
           +S P+  W L+ L T GC     P  S+G
Sbjct: 224 VSFPLRSWPLWYLSTRGCSCTSSPKESVG 138


>AA660656 weakly similar to GP|6498428|dbj| Similar to Arabidopsis thaliana
           chromosome II BAC T27A16 sequence; hypothetical protein.
           (AC005496), partial (14%)
          Length = 634

 Score = 27.3 bits (59), Expect = 2.8
 Identities = 12/33 (36%), Positives = 19/33 (57%)
 Frame = +3

Query: 76  GCGLPPGPGGSIGALEGVSYLVVVGIVAWSIYT 108
           G  L   PGG +  + G   ++VVG++ WS+ T
Sbjct: 357 GYALSQLPGGWLAKIFGGXNVLVVGVLIWSVAT 455


>BQ150300 similar to GP|10178184|dbj 50S ribosomal protein L29 {Arabidopsis
           thaliana}, partial (51%)
          Length = 695

 Score = 26.9 bits (58), Expect = 3.6
 Identities = 15/37 (40%), Positives = 24/37 (64%)
 Frame = +3

Query: 109 KTKTGSGLPNGPFGLLGAVEGLSYLALVAIVVVFGLQ 145
           K K G G+PN P+ L   ++ LS LA++ I ++F L+
Sbjct: 474 KLKGGPGIPNSPYMLSSELQ-LS-LAVIYITIIFRLE 578


>BF633702 
          Length = 361

 Score = 26.2 bits (56), Expect = 6.2
 Identities = 10/36 (27%), Positives = 19/36 (52%)
 Frame = -2

Query: 46  NSSPTEIAAIAGGLISTPVIGWSLYTLKTTGCGLPP 81
           +S+ +EI A+       P++   +Y+ K  G  +PP
Sbjct: 309 DSTTSEILAVIPSFA*PPIVPMQMYSAKAVGSAVPP 202


>TC78434 similar to GP|3790100|gb|AAC67586.1| pyrophosphate-dependent
          phosphofructokinase beta subunit {Citrus x paradisi},
          partial (49%)
          Length = 1050

 Score = 25.8 bits (55), Expect = 8.1
 Identities = 13/36 (36%), Positives = 17/36 (47%)
 Frame = +3

Query: 2  LLFSPISTPKTFTNHLNHPLRKPKSHHVRLKLKITN 37
          L  + IS    F+N L+HPL K    H R    + N
Sbjct: 9  LFLNLISLVVPFSNLLSHPLSKTNLRHSRFSTLLNN 116


>TC91118 
          Length = 766

 Score = 25.8 bits (55), Expect = 8.1
 Identities = 10/28 (35%), Positives = 16/28 (56%)
 Frame = +3

Query: 22  RKPKSHHVRLKLKITNMAKEGSDTNSSP 49
           RKP++HH+R  L +  +A+  S     P
Sbjct: 561 RKPRTHHLRKSLSVVRLAEITSGLRLRP 644


>TC85214 similar to PIR|S26688|S26688 legumin K - garden pea, partial (94%)
          Length = 1831

 Score = 25.8 bits (55), Expect = 8.1
 Identities = 13/63 (20%), Positives = 30/63 (46%)
 Frame = -3

Query: 80   PPGPGGSIGALEGVSYLVVVGIVAWSIYTKTKTGSGLPNGPFGLLGAVEGLSYLALVAIV 139
            PP    ++G +  +S+ + +  ++W   +     SG+   PF     + G+S++ L   +
Sbjct: 1121 PPWTTTTVGIIFSLSFFLTMSGMSWVSLSFFLAMSGMSWIPFSFFLTMSGMSWVPLSFFL 942

Query: 140  VVF 142
             +F
Sbjct: 941  TMF 933


>TC81332 similar to GP|15810167|gb|AAL06985.1 At1g64110/F22C12_22
           {Arabidopsis thaliana}, partial (39%)
          Length = 1514

 Score = 25.8 bits (55), Expect = 8.1
 Identities = 10/27 (37%), Positives = 18/27 (66%)
 Frame = +2

Query: 7   ISTPKTFTNHLNHPLRKPKSHHVRLKL 33
           I+ PK+  N L + L   +SHH++++L
Sbjct: 278 ITVPKSMKNSLCYSLTT*RSHHLKMRL 358


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.318    0.140    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,425,622
Number of Sequences: 36976
Number of extensions: 71030
Number of successful extensions: 489
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 482
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 487
length of query: 163
length of database: 9,014,727
effective HSP length: 89
effective length of query: 74
effective length of database: 5,723,863
effective search space: 423565862
effective search space used: 423565862
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)


Medicago: description of AC147178.12