BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147012.12 + phase: 0
(750 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AL376912 similar to GP|15623889|dbj contains EST C98124(C0742)~u... 330 2e-90
TC92063 similar to PIR|H71423|H71423 hypothetical protein dl3960... 142 7e-59
TC93454 similar to PIR|H71423|H71423 hypothetical protein dl3960... 219 2e-57
TC88544 similar to PIR|D96838|D96838 unknown protein T21F11.5 [i... 34 0.24
TC78091 similar to GP|17104531|gb|AAL34154.1 unknown protein {Ar... 33 0.53
BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xy... 30 2.6
AL368332 weakly similar to GP|21322711|e pherophorin-dz1 protein... 30 2.6
TC84308 similar to PIR|T47773|T47773 hypothetical protein F24I3.... 30 3.4
BG454595 similar to PIR|D86323|D863 protein F14D16.9 [imported] ... 30 4.5
TC83825 similar to GP|13374868|emb|CAC34502. putative protein {A... 30 4.5
CA916991 similar to GP|15706301|dbj THH1 {Arabidopsis thaliana},... 30 4.5
TC91652 similar to GP|11935197|gb|AAG42014.1 unknown protein {Ar... 30 4.5
TC82317 similar to GP|2921209|gb|AAC04691.1| beta-ketoacyl-ACP s... 29 5.8
AW736664 29 5.8
TC76386 similar to GP|12049598|emb|CAC19855. mitochondrial succi... 29 7.6
CB066657 similar to PIR|T45858|T45 hypothetical protein F3A4.130... 28 9.9
TC93044 weakly similar to PIR|T47960|T47960 hypothetical protein... 28 9.9
TC93128 similar to GP|20160696|dbj|BAB89639. hypothetical protei... 28 9.9
BG644737 similar to GP|14596153|gb| Unknown protein {Arabidopsis... 28 9.9
>AL376912 similar to GP|15623889|dbj contains EST C98124(C0742)~unknown
protein {Oryza sativa (japonica cultivar-group)},
partial (22%)
Length = 490
Score = 330 bits (845), Expect = 2e-90
Identities = 161/162 (99%), Positives = 161/162 (99%)
Frame = -3
Query: 426 NGSFRPVRVHVRGAIDGLAGIGRGTTFVPAAASPPTRFVFSRVPFGVGNRNYPQSAANDD 485
NGSFRPVRVH RGAIDGLAGIGRGTTFVPAAASPPTRFVFSRVPFGVGNRNYPQSAANDD
Sbjct: 488 NGSFRPVRVHARGAIDGLAGIGRGTTFVPAAASPPTRFVFSRVPFGVGNRNYPQSAANDD 309
Query: 486 SEARADHNGDLSGDGLTALVGLSQGGNYGTNIHTELTQREQEMGLQSTAGGASTGGIPVQ 545
SEARADHNGDLSGDGLTALVGLSQGGNYGTNIHTELTQREQEMGLQSTAGGASTGGIPVQ
Sbjct: 308 SEARADHNGDLSGDGLTALVGLSQGGNYGTNIHTELTQREQEMGLQSTAGGASTGGIPVQ 129
Query: 546 LLETPEHTIGIEWENDNSSSISLDLKTPLSHFPPFRFGVSFE 587
LLETPEHTIGIEWENDNSSSISLDLKTPLSHFPPFRFGVSFE
Sbjct: 128 LLETPEHTIGIEWENDNSSSISLDLKTPLSHFPPFRFGVSFE 3
>TC92063 similar to PIR|H71423|H71423 hypothetical protein dl3960w -
Arabidopsis thaliana, partial (13%)
Length = 769
Score = 142 bits (358), Expect(2) = 7e-59
Identities = 70/70 (100%), Positives = 70/70 (100%)
Frame = +1
Query: 681 VDSREKVTARYQLTCPSKREMLVFGSFKQTGTLLPKAPKGWGWRTALLFDELADLLQNGA 740
VDSREKVTARYQLTCPSKREMLVFGSFKQTGTLLPKAPKGWGWRTALLFDELADLLQNGA
Sbjct: 172 VDSREKVTARYQLTCPSKREMLVFGSFKQTGTLLPKAPKGWGWRTALLFDELADLLQNGA 351
Query: 741 LRVIAVVQLV 750
LRVIAVVQLV
Sbjct: 352 LRVIAVVQLV 381
Score = 104 bits (259), Expect(2) = 7e-59
Identities = 56/87 (64%), Positives = 56/87 (64%)
Frame = +3
Query: 594 DGQVKHSPEVFYAGSLWKVSIQAFNDEDPQGRRTLGNVFITSKLSPSGCEQISAIVIIVN 653
DGQVKHSPEVFYAGSLWKVSIQAFNDEDPQGRRTL
Sbjct: 3 DGQVKHSPEVFYAGSLWKVSIQAFNDEDPQGRRTL------------------------- 107
Query: 654 SLNDNAGLFLHRRKAEITDVHRKVHMY 680
GLFLHRRKAEITDVHRKVHMY
Sbjct: 108 ------GLFLHRRKAEITDVHRKVHMY 170
>TC93454 similar to PIR|H71423|H71423 hypothetical protein dl3960w -
Arabidopsis thaliana, partial (6%)
Length = 362
Score = 219 bits (559), Expect = 2e-57
Identities = 103/106 (97%), Positives = 106/106 (99%)
Frame = +3
Query: 256 NRLVRDQSRDSFSSSSCSGLTSSEWGRYGTPLLSWGGHVGRRQVKAHPRGNYRDEDDVFI 315
NRLVRDQSRDSFSSSSCSGLTSSEWGRYGTPLLSWGGHVGRRQVKAHPRGNYRDEDDVFI
Sbjct: 3 NRLVRDQSRDSFSSSSCSGLTSSEWGRYGTPLLSWGGHVGRRQVKAHPRGNYRDEDDVFI 182
Query: 316 NIFEGGSLLYCNMSFDALLNVRKQLEEIGFPCKAVNDGLWLQMLLS 361
NIFEGGSLLYCNMSFDALLNVRKQLEEIGFPCKAVNDGLWLQ++L+
Sbjct: 183 NIFEGGSLLYCNMSFDALLNVRKQLEEIGFPCKAVNDGLWLQVVLT 320
>TC88544 similar to PIR|D96838|D96838 unknown protein T21F11.5 [imported] -
Arabidopsis thaliana, partial (30%)
Length = 1668
Score = 33.9 bits (76), Expect = 0.24
Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 17/58 (29%)
Frame = +3
Query: 1 MENPQYPN---------NPPPSPYNQRSASIPKQPPS--------HHSDNNDPTTAEL 41
+ PQ PN +PPP+P +A+ K PPS HS+NN P T L
Sbjct: 54 VSKPQNPNPTLHHSTTSSPPPTPATMTAATATKDPPSPSPYAKSEAHSNNNQPQTQHL 227
>TC78091 similar to GP|17104531|gb|AAL34154.1 unknown protein {Arabidopsis
thaliana}, partial (52%)
Length = 1863
Score = 32.7 bits (73), Expect = 0.53
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = +2
Query: 10 PPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRALDCNLA 49
PPPS YNQR +Q SH S+ + +T L NL+
Sbjct: 1388 PPPSKYNQRQQFFEQQGSSHSSNGSSSSTDSLLGQTQNLS 1507
>BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xylostella
granulovirus}, partial (42%)
Length = 909
Score = 30.4 bits (67), Expect = 2.6
Identities = 14/36 (38%), Positives = 20/36 (54%)
Frame = +2
Query: 3 NPQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDPTT 38
+P +P+ PPP+P+ P PP +H N PTT
Sbjct: 218 HPHHPHTPPPTPH-------PPNPPPNHP--NHPTT 298
>AL368332 weakly similar to GP|21322711|e pherophorin-dz1 protein {Volvox
carteri f. nagariensis}, partial (10%)
Length = 478
Score = 30.4 bits (67), Expect = 2.6
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +1
Query: 3 NPQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDP 36
+P +P NPPPSP++ P PP HS + P
Sbjct: 103 SPFHPINPPPSPFH------PFNPPPPHSIKSPP 186
>TC84308 similar to PIR|T47773|T47773 hypothetical protein F24I3.210 -
Arabidopsis thaliana, partial (42%)
Length = 755
Score = 30.0 bits (66), Expect = 3.4
Identities = 15/32 (46%), Positives = 20/32 (61%), Gaps = 1/32 (3%)
Frame = +2
Query: 61 NSGSFSDIVVNAMGSTYHLHRLIL-SRSSYFR 91
N +FSD+V + G H HR IL +RS +FR
Sbjct: 146 NGQAFSDVVFSVEGRLVHAHRCILAARSLFFR 241
>BG454595 similar to PIR|D86323|D863 protein F14D16.9 [imported] -
Arabidopsis thaliana, partial (34%)
Length = 669
Score = 29.6 bits (65), Expect = 4.5
Identities = 15/40 (37%), Positives = 20/40 (49%)
Frame = +2
Query: 4 PQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRA 43
P+ P PPP+ + +R KQPP HH + T RA
Sbjct: 161 PKIPPPPPPTRFPRR-----KQPPLHHLRPREATVLPARA 265
>TC83825 similar to GP|13374868|emb|CAC34502. putative protein {Arabidopsis
thaliana}, partial (12%)
Length = 558
Score = 29.6 bits (65), Expect = 4.5
Identities = 23/82 (28%), Positives = 37/82 (45%), Gaps = 1/82 (1%)
Frame = -1
Query: 210 LGNSLSDPDGVRTSCYVEVPL-GAGTTTGVGIEGPSEEGSCYHSDNNNRLVRDQSRDSFS 268
L N ++DP + ++ P A +T+ I GS + S N + + SFS
Sbjct: 345 LSNDINDPFSITL---LDPPSPSASSTSSSSISSSYSYGSSFSSSNPSNSSSNSPTPSFS 175
Query: 269 SSSCSGLTSSEWGRYGTPLLSW 290
SS S ++ S + + T L SW
Sbjct: 174 FSSFSPISWS*FAKVRTSLPSW 109
>CA916991 similar to GP|15706301|dbj THH1 {Arabidopsis thaliana}, partial
(16%)
Length = 784
Score = 29.6 bits (65), Expect = 4.5
Identities = 11/24 (45%), Positives = 16/24 (65%)
Frame = -1
Query: 559 ENDNSSSISLDLKTPLSHFPPFRF 582
+N NS +D+KTP++HFP F
Sbjct: 598 QNSNSFPFYMDIKTPINHFPRENF 527
>TC91652 similar to GP|11935197|gb|AAG42014.1 unknown protein {Arabidopsis
thaliana}, partial (78%)
Length = 887
Score = 29.6 bits (65), Expect = 4.5
Identities = 14/36 (38%), Positives = 22/36 (60%)
Frame = +1
Query: 9 NPPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRAL 44
NPPP +SA + + P S ++N+D T E++AL
Sbjct: 604 NPPPIQ-EMKSADVKETPESSSANNSDHTNLEMQAL 708
>TC82317 similar to GP|2921209|gb|AAC04691.1| beta-ketoacyl-ACP synthase I
{Perilla frutescens}, partial (35%)
Length = 717
Score = 29.3 bits (64), Expect = 5.8
Identities = 19/73 (26%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
Frame = +2
Query: 2 ENPQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRALDCNLASLCE--HVQIEG 59
+NP PPP P +S+P PP + P R++ +LA + +
Sbjct: 113 KNPPMRPPPPPEPQRDSPSSLPPSPPPLLKERRIP-----RSVS*SLAWVASLYMATMST 277
Query: 60 FNSGSFSDIVVNA 72
F++ SFS ++V +
Sbjct: 278 FSTKSFSPVIVGS 316
>AW736664
Length = 281
Score = 29.3 bits (64), Expect = 5.8
Identities = 15/41 (36%), Positives = 23/41 (55%)
Frame = -2
Query: 54 HVQIEGFNSGSFSDIVVNAMGSTYHLHRLILSRSSYFRFEL 94
H + + F S +F+ +VV+A + HLH LI + FEL
Sbjct: 124 HNKEKVFTSNNFASLVVSATTNQIHLH*LISTSKHVXEFEL 2
>TC76386 similar to GP|12049598|emb|CAC19855. mitochondrial succinate
dehydrogenase iron-sulphur subunit {Arabidopsis
thaliana}, partial (60%)
Length = 707
Score = 28.9 bits (63), Expect = 7.6
Identities = 15/35 (42%), Positives = 17/35 (47%), Gaps = 2/35 (5%)
Frame = +2
Query: 4 PQYPNNPPPSP--YNQRSASIPKQPPSHHSDNNDP 36
P P NPPPSP + S+P Q P HH P
Sbjct: 110 PNLPTNPPPSPCLRSPSPTSLP-QSPRHHRPKKIP 211
>CB066657 similar to PIR|T45858|T45 hypothetical protein F3A4.130 -
Arabidopsis thaliana, partial (25%)
Length = 782
Score = 28.5 bits (62), Expect = 9.9
Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 3 NPQYPN-NPPPSPYNQRSASIPKQPPSHHSDNNDP 36
N +PN NPP P+++R IP PP + ++ P
Sbjct: 102 NSPHPNLNPPHYPHHRRRHRIPP*PPPRYDSSSIP 206
>TC93044 weakly similar to PIR|T47960|T47960 hypothetical protein F15G16.70
- Arabidopsis thaliana, partial (24%)
Length = 764
Score = 28.5 bits (62), Expect = 9.9
Identities = 24/78 (30%), Positives = 38/78 (47%)
Frame = +1
Query: 140 NKMLETDLGKMSLKSDLKDTSMPNLLVELGDSEGDFNSDICDSNQRVQLASYDISPNLNP 199
NK+L T LGK+ + + TS P+ L+ + + +S IC + RV +S LN
Sbjct: 214 NKLLYTPLGKIFIIQPDEMTSPPHPLLPSESAFYELDSTICGYSPRV------LSSFLNQ 375
Query: 200 SYPSDMEGPSLGNSLSDP 217
+P + +LSDP
Sbjct: 376 PHPIE--------TLSDP 405
>TC93128 similar to GP|20160696|dbj|BAB89639. hypothetical protein~similar
to Arabidopsis thaliana chromosome 4 At4g37510, partial
(23%)
Length = 673
Score = 28.5 bits (62), Expect = 9.9
Identities = 11/26 (42%), Positives = 13/26 (49%)
Frame = +1
Query: 269 SSSCSGLTSSEWGRYGTPLLSWGGHV 294
S SC G+ WGR G L W G +
Sbjct: 583 SFSCRGIRGRRWGR*GRGFLDWWGSI 660
>BG644737 similar to GP|14596153|gb| Unknown protein {Arabidopsis thaliana},
partial (19%)
Length = 471
Score = 28.5 bits (62), Expect = 9.9
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +2
Query: 393 GSTTGSYIQEHNHNNMPGGGGNIYVAESSTGERNG 427
GSTTG++ H +N PG G + S G +G
Sbjct: 62 GSTTGAHTPVHTLSNFPGPKGEYLGSRSPMGRTSG 166
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.134 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,075,185
Number of Sequences: 36976
Number of extensions: 392446
Number of successful extensions: 2721
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 2439
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2692
length of query: 750
length of database: 9,014,727
effective HSP length: 103
effective length of query: 647
effective length of database: 5,206,199
effective search space: 3368410753
effective search space used: 3368410753
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC147012.12