BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146865.13 - phase: 0
(592 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC91820 homologue to GP|23617110|dbj|BAC20792. contains ESTs C27... 389 e-160
BG647512 similar to PIR|B84924|B8 hypothetical protein At2g48160... 92 4e-19
TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1 ... 34 0.14
TC82106 similar to PIR|T49191|T49191 hypothetical protein MAA21.... 32 0.68
TC84579 31 1.2
TC88548 similar to GP|20196934|gb|AAB86455.2 bZIP family transcr... 30 2.0
TC88793 similar to PIR|T14294|T14294 polycomb-like protein - car... 30 2.6
AJ498702 similar to PIR|T01743|T01 ribosomal protein L10 chloro... 30 2.6
BE248178 similar to PIR|G86270|G862 hypothetical protein AAF8130... 30 3.4
TC77857 29 4.4
TC81143 weakly similar to PIR|T08562|T08562 hypothetical protein... 29 4.4
AW683488 similar to GP|12324581|gb hypothetical protein; 30600-2... 29 5.8
AJ500960 28 7.6
TC83719 similar to GP|18143763|dbj|BAB79812. hypothetical protei... 28 7.6
TC88373 weakly similar to GP|21553792|gb|AAM62885.1 unknown {Ara... 28 7.6
TC88169 similar to GP|20259099|gb|AAM14265.1 putative ubiquitin-... 28 7.6
TC77086 homologue to SP|P16048|GCSH_PEA Glycine cleavage system ... 28 7.6
AW689511 similar to GP|14140155|emb putative AP2 domain transcri... 28 9.9
TC79680 weakly similar to GP|21751020|dbj|BAC03887. unnamed prot... 28 9.9
NP565645 NP565645|AY115840.1|AAM55301.1 auxin efflux carrier pro... 28 9.9
>TC91820 homologue to GP|23617110|dbj|BAC20792. contains ESTs C27700(C52636)
AU222511(C52636)~transcription factor (PWWP domain
protein) -like, partial (3%)
Length = 870
Score = 389 bits (998), Expect(2) = e-160
Identities = 192/193 (99%), Positives = 192/193 (99%)
Frame = +3
Query: 296 SSNCENRGSADVGSSVLIGTMDNVSPSKNFTPCFENQVCNTKKDQTFCSVDCEAALPPSK 355
SSNCENRGSADVGSSVLIGTMDNVSPSKNFTPCFENQVCNTKKDQTFCSVDCEAALPPSK
Sbjct: 288 SSNCENRGSADVGSSVLIGTMDNVSPSKNFTPCFENQVCNTKKDQTFCSVDCEAALPPSK 467
Query: 356 RLHRALEAMSANAAEEGQAHVESSASRMTSIATCCISSIKTSPDVAINDHEGGGLELQKF 415
RLHRALEAMSANAAEEGQAHVESSASRMTSIATCCISSIKTSPDVAINDHEGGGLELQKF
Sbjct: 468 RLHRALEAMSANAAEEGQAHVESSASRMTSIATCCISSIKTSPDVAINDHEGGGLELQKF 647
Query: 416 DACGGGDSSHIIVHSISANSNPMISTENKLSNQVDEPSTRFQPQETGKNVLQCAADQIEE 475
DACGGGDSSHIIVHSISANSNPMISTENKLSNQVDEPSTRFQPQETGKNVLQCAADQIEE
Sbjct: 648 DACGGGDSSHIIVHSISANSNPMISTENKLSNQVDEPSTRFQPQETGKNVLQCAADQIEE 827
Query: 476 LSDFVVSHTANVD 488
L DFVVSHTANVD
Sbjct: 828 LXDFVVSHTANVD 866
Score = 195 bits (496), Expect(2) = e-160
Identities = 94/95 (98%), Positives = 94/95 (98%)
Frame = +2
Query: 201 RNPNRKRATNDTSKPTIRVEEELGVRNSSQSSQNICRNSEERCFEQDGDEHLPLVKRWRV 260
RNPNRKRATNDTSKPTIRVEEELGVRNSSQSSQNIC NSEERCFEQDGDEHLPLVKRWRV
Sbjct: 2 RNPNRKRATNDTSKPTIRVEEELGVRNSSQSSQNICINSEERCFEQDGDEHLPLVKRWRV 181
Query: 261 RMGKSSSTEGELNSIPHTPGKSCKEDINSPPQMIA 295
RMGKSSSTEGELNSIPHTPGKSCKEDINSPPQMIA
Sbjct: 182 RMGKSSSTEGELNSIPHTPGKSCKEDINSPPQMIA 286
>BG647512 similar to PIR|B84924|B8 hypothetical protein At2g48160 [imported]
- Arabidopsis thaliana, partial (7%)
Length = 596
Score = 92.4 bits (228), Expect = 4e-19
Identities = 45/47 (95%), Positives = 45/47 (95%)
Frame = +2
Query: 1 MKSSNSDMDSHGLVCPAEDDSAAVLKDESHDNEASKELTENVASVHS 47
MKSSNSDMDSHGLVCPAEDDSAAVLKDESHDNEASKELTE ASVHS
Sbjct: 455 MKSSNSDMDSHGLVCPAEDDSAAVLKDESHDNEASKELTEKCASVHS 595
>TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1
{Arabidopsis thaliana}, partial (47%)
Length = 1538
Score = 34.3 bits (77), Expect = 0.14
Identities = 39/196 (19%), Positives = 79/196 (39%), Gaps = 5/196 (2%)
Frame = +3
Query: 25 LKDESHDNEASKELTENVASVHSAKPLTYSSRKRSAAELCPQGFITDRHMPVRKNRSSSR 84
+K ++ S+E E+ + + + +Y + S +E + + DR RK+R S
Sbjct: 528 IKQKAKSESESEESKESDSELRKKRRKSYKKSRESDSESESESEVEDRKR--RKSRKYSE 701
Query: 85 VQPFMFPCNDSGKNAGSQLTNAAQGASVRRNKRLRKSP-----DLAGCNDFDSSALVLNG 139
+ K + ++ + S R+ +R S D +G +D
Sbjct: 702 SDSDTNSEEEDRKRRRRRKSSRRRRKSSRKKRRCSDSDESETDDESGYDDSGRKRKRSKR 881
Query: 140 SMEDKDNSSEILTNDSDEFSLNEGSAMDSNFKHTETSECPEEVELNKGLDLKIKGVVNKK 199
S + K SSE ++ S+E L SA+ + +E ++V+ K ++ + + K+
Sbjct: 882 SRKSKKKSSEPVSEGSEEIELGSDSAV------AKINEEIDDVDEEKMAEMNAEALKLKE 1043
Query: 200 KRNPNRKRATNDTSKP 215
+K A +D P
Sbjct: 1044LFESQKKPALDDDEPP 1091
>TC82106 similar to PIR|T49191|T49191 hypothetical protein MAA21.130 -
Arabidopsis thaliana, partial (5%)
Length = 990
Score = 32.0 bits (71), Expect = 0.68
Identities = 30/164 (18%), Positives = 71/164 (43%), Gaps = 14/164 (8%)
Frame = +2
Query: 97 KNAGSQLTNAAQGASVRRNKRLRKSPDLAGCNDFDSSALV--------LNGSMEDKDNSS 148
K++ S+ + + G V++++ + + ++ + L L + +D+ S
Sbjct: 95 KDSESEQSKSVSGVEVKKSEEMLQQVQSGSGSEMEEGELEPEPVRETELKPAPKDEAAGS 274
Query: 149 EILTNDSDEFSL---NEGSAMDSN--FKHTETSECPEEVELNKGLDLKIKGVVNKKKRNP 203
EI D+ + NE + D++ + +T EE + + +D ++K + P
Sbjct: 275 EIQQTSEDKQAQKKKNECHSGDADVVMEEKQTLSSKEEAKCTQDIDSEVKVAGKEVCELP 454
Query: 204 NRKRATNDTSKPTIRVEEELGVRNSSQSSQNICRNSEE-RCFEQ 246
+ +D + E E+G ++ +N+C N ++ RC E+
Sbjct: 455 ---KTQDDPTNEISVAESEIGTTSNVDDKKNVCLNGDDTRCKEE 577
>TC84579
Length = 698
Score = 31.2 bits (69), Expect = 1.2
Identities = 31/123 (25%), Positives = 56/123 (45%), Gaps = 2/123 (1%)
Frame = +1
Query: 93 NDSGKNAGSQLTNAAQGASVRRNKRLRKSPDLAGCNDFDSSALVLNGSMEDKDNSSEILT 152
++ GK Q T Q R K+ +K+ L + S A + GSM+ ++ L
Sbjct: 151 SEEGKMLFKQQTKQLQVDEEERIKK-QKAKSLVRSEEGRSRAEAVEGSMQKVYAANSPLQ 327
Query: 153 NDSDEFSLNEGSAMDSNFKHTETSECPEEVELNKGLDLKIKGVVNKKKRNPNRK--RATN 210
N +EF +E +A +SE P+ + + + VV+K++R+ +K R+ +
Sbjct: 328 NKQEEFQPSESAAALGKSGAANSSEMPDASDALQAQN----NVVSKQRRSYKQKHNRSLS 495
Query: 211 DTS 213
TS
Sbjct: 496 KTS 504
>TC88548 similar to GP|20196934|gb|AAB86455.2 bZIP family transcription
factor {Arabidopsis thaliana}, partial (34%)
Length = 1570
Score = 30.4 bits (67), Expect = 2.0
Identities = 38/151 (25%), Positives = 61/151 (40%), Gaps = 23/151 (15%)
Frame = +2
Query: 425 HIIVHSISANSNPMISTENKLSNQVDEPSTRFQPQ-----ETGKN------VLQCAADQ- 472
++I+HSI A+ M S + + + E + E G+N V + +A+Q
Sbjct: 620 NLIIHSIMASEKAMASQDAQGKKEKSETGLAIRDSALAIPEVGRNRGQHPNVYRVSAEQR 799
Query: 473 -------IEELSDFVVSHTANVDLKTQVH-GETYPDLDS-KCNEAESNQDSPALS--LPP 521
+ L D + S + ++ G P L S C E SPA +P
Sbjct: 800 RAIGSGSTKTLKDHMKSSATDGKMQQWFREGIAGPMLSSGMCTEVFQFDVSPAPGAIVPA 979
Query: 522 NIEANIITSNHSNTTSNASEHNRINLHSVAD 552
ANI + N N+T+ NR LH++ D
Sbjct: 980 AAVANISSENRQNSTNVNKSRNRRILHTLPD 1072
>TC88793 similar to PIR|T14294|T14294 polycomb-like protein - carrot,
partial (23%)
Length = 1184
Score = 30.0 bits (66), Expect = 2.6
Identities = 22/83 (26%), Positives = 37/83 (44%), Gaps = 8/83 (9%)
Frame = +1
Query: 48 AKPLTYSSRKRSAAELCPQGFI--------TDRHMPVRKNRSSSRVQPFMFPCNDSGKNA 99
AKPL +R+ + L PQ + +H+P + +S+ Q +G +
Sbjct: 370 AKPLRKRTRRTTTTHLPPQTTNAHPTPLPNSQQHIPHQNQPTSNLPQQTPIHAQSNGNSL 549
Query: 100 GSQLTNAAQGASVRRNKRLRKSP 122
+ TN + ASV+R K+ SP
Sbjct: 550 HRRGTNRRKSASVKRFKKDESSP 618
>AJ498702 similar to PIR|T01743|T01 ribosomal protein L10 chloroplast -
common tobacco, partial (76%)
Length = 663
Score = 30.0 bits (66), Expect = 2.6
Identities = 24/72 (33%), Positives = 35/72 (48%), Gaps = 1/72 (1%)
Frame = +1
Query: 193 KGVVNKKKRNP-NRKRATNDTSKPTIRVEEELGVRNSSQSSQNICRNSEERCFEQDGDEH 251
+ +K++RN NRKR T P + L + S++S+N N+E RC E EH
Sbjct: 151 RNFTHKERRNS*NRKRTT*KLLPPRRNQLQRLHRKTISRTSKNSS*NNETRCCE----EH 318
Query: 252 LPLVKRWRVRMG 263
+ L R MG
Sbjct: 319 VGL*ST*RNTMG 354
>BE248178 similar to PIR|G86270|G862 hypothetical protein AAF81300.1
[imported] - Arabidopsis thaliana, partial (5%)
Length = 411
Score = 29.6 bits (65), Expect = 3.4
Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 1/70 (1%)
Frame = +1
Query: 263 GKSSSTEGELNSIPHTPGKSCKEDINSPPQMIASSNCENRGSAD-VGSSVLIGTMDNVSP 321
G SSS+ E++S P SC E I SP ++ + + R S D VGSS T +N++
Sbjct: 124 GGSSSSLSEMDSKPFLGSSSCGE-IRSP---ASNQSLQERSSQDAVGSSGGPKTNENLTR 291
Query: 322 SKNFTPCFEN 331
+ + T EN
Sbjct: 292 TTSRTEVMEN 321
>TC77857
Length = 558
Score = 29.3 bits (64), Expect = 4.4
Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 4/59 (6%)
Frame = +1
Query: 519 LPPNIEANIITSNHSNTTSNASEHNR----INLHSVADVMKKEIISPNLDPPRNEVVIS 573
L PN +N + +NT H+ IN+H + M ++II+PN P+ E++IS
Sbjct: 163 LLPNPISNTGENFSNNTLHPLMVHSLVVEDINIHEIISSMVEKIITPNCYVPKLELLIS 339
>TC81143 weakly similar to PIR|T08562|T08562 hypothetical protein T22F8.40 -
Arabidopsis thaliana, partial (27%)
Length = 988
Score = 29.3 bits (64), Expect = 4.4
Identities = 25/99 (25%), Positives = 43/99 (43%), Gaps = 7/99 (7%)
Frame = +2
Query: 116 KRLRKSPDLAGCNDFDSSAL-------VLNGSMEDKDNSSEILTNDSDEFSLNEGSAMDS 168
K+ RK + A + DS A +L+ ++D D+ S + D+ N+G MDS
Sbjct: 317 KQTRKLFEKAMKAEMDSKARNKKSRNQILDNEIDDDDDDSVVFDQFKDKRRQNKGPRMDS 496
Query: 169 NFKHTETSECPEEVELNKGLDLKIKGVVNKKKRNPNRKR 207
+ +S P L++ KG + +P RK+
Sbjct: 497 SSSKRSSSGKP---FLSRHFSFGSKGSKSMLDNHPTRKK 604
>AW683488 similar to GP|12324581|gb hypothetical protein; 30600-24965
{Arabidopsis thaliana}, partial (11%)
Length = 624
Score = 28.9 bits (63), Expect = 5.8
Identities = 36/155 (23%), Positives = 60/155 (38%), Gaps = 10/155 (6%)
Frame = +1
Query: 291 PQMIASSNCENRGSADVGSSVLIGTMDNVSPSKNFTPCFENQVCNTKKDQTF----CSVD 346
P +A+S C+N GS + SS + T N CF Q TKK T+ VD
Sbjct: 37 PPSLATSQCKN*GSIHLSSSTKVHTNANSGRKALIHFCFRFQ-SGTKKMPTYKIRGIDVD 213
Query: 347 CEAALPPSKRLHRALEAMSANAAEEGQAHVESSASRMTSIATCCI------SSIKTSPDV 400
S+ ++ ++ + + EE A +ES ++ C S S +
Sbjct: 214 FPYEAYDSQLVY--MDKVMQSLQEESNALLESPTGTGKTLCLLCATLAWRKSLGSFSTGM 387
Query: 401 AINDHEGGGLELQKFDACGGGDSSHIIVHSISANS 435
+I + G E+ + G IV++ +S
Sbjct: 388 SIKTGDNGKTEVSSSQSEPGASKFPTIVYASRTHS 492
>AJ500960
Length = 552
Score = 28.5 bits (62), Expect = 7.6
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +3
Query: 545 INLHSVADVMKKEIISPNLDPPRNEVVIS 573
IN+H + M ++II+PN P+ E++IS
Sbjct: 333 INIHEIISSMVEKIITPNCYVPKLELLIS 419
>TC83719 similar to GP|18143763|dbj|BAB79812. hypothetical protein
{Clostridium perfringens str. 13}, partial (7%)
Length = 542
Score = 28.5 bits (62), Expect = 7.6
Identities = 13/34 (38%), Positives = 17/34 (49%)
Frame = -1
Query: 519 LPPNIEANIITSNHSNTTSNASEHNRINLHSVAD 552
LPPN+ +NI T S SEHN + + D
Sbjct: 470 LPPNMVSNIEEHGKMETQSEGSEHNSMRKNKTQD 369
>TC88373 weakly similar to GP|21553792|gb|AAM62885.1 unknown {Arabidopsis
thaliana}, partial (25%)
Length = 970
Score = 28.5 bits (62), Expect = 7.6
Identities = 18/56 (32%), Positives = 32/56 (57%), Gaps = 1/56 (1%)
Frame = -1
Query: 182 VELNKGLDLKIKGVVNKKKRNPNRKRATNDTSKPTIRVEEELGVRNSSQSSQN-IC 236
+ELNK L ++ + ++ +K N TND + +RVEE +G ++ +Q+ IC
Sbjct: 952 IELNKILSIQKQLNMDSRK---NEMIITNDFQEAALRVEERVGYKSKLPMTQSKIC 794
>TC88169 similar to GP|20259099|gb|AAM14265.1 putative ubiquitin-conjugating
enzyme E2 {Arabidopsis thaliana}, partial (23%)
Length = 1824
Score = 28.5 bits (62), Expect = 7.6
Identities = 24/77 (31%), Positives = 34/77 (43%)
Frame = -2
Query: 249 DEHLPLVKRWRVRMGKSSSTEGELNSIPHTPGKSCKEDINSPPQMIASSNCENRGSADVG 308
D LP K+W + S T LN K ++ S ++ +S CE S D
Sbjct: 905 DNPLPSSKKWW-SSEQFSITSNCLNWFELLLSKESEKSFLS--WILITSGCEVLSSVDCN 735
Query: 309 SSVLIGTMDNVSPSKNF 325
SSV G+M + SK+F
Sbjct: 734 SSVGCGSMQKLQVSKSF 684
>TC77086 homologue to SP|P16048|GCSH_PEA Glycine cleavage system H protein
mitochondrial precursor. [Garden pea] {Pisum sativum},
complete
Length = 859
Score = 28.5 bits (62), Expect = 7.6
Identities = 15/43 (34%), Positives = 25/43 (57%)
Frame = -1
Query: 362 EAMSANAAEEGQAHVESSASRMTSIATCCISSIKTSPDVAIND 404
E++ A A EE AH+ S+ + TS + CC+ T+ + IN+
Sbjct: 172 ESLRALAVEE--AHILSAIVKTTSASLCCVCDKSTNNNKNINE 50
>AW689511 similar to GP|14140155|emb putative AP2 domain transcription factor
{Oryza sativa}, partial (32%)
Length = 703
Score = 28.1 bits (61), Expect = 9.9
Identities = 20/76 (26%), Positives = 33/76 (43%)
Frame = +1
Query: 516 ALSLPPNIEANIITSNHSNTTSNASEHNRINLHSVADVMKKEIISPNLDPPRNEVVISEG 575
+L PP +N+ HS++ SN HN +L ++ S PP + +
Sbjct: 43 SLFAPPTTFSNLF-HKHSHSNSNY-HHNNNSLTTMTQSTTTSSTSSQESPPTHSIQKQSP 216
Query: 576 TKCLKPAVDDVNRAND 591
T+ +P DD N N+
Sbjct: 217 TRPKRPRDDDNNDNNN 264
>TC79680 weakly similar to GP|21751020|dbj|BAC03887. unnamed protein product
{Homo sapiens}, partial (13%)
Length = 2636
Score = 28.1 bits (61), Expect = 9.9
Identities = 21/82 (25%), Positives = 34/82 (40%)
Frame = -3
Query: 256 KRWRVRMGKSSSTEGELNSIPHTPGKSCKEDINSPPQMIASSNCENRGSADVGSSVLIGT 315
K W +R S++ E + + K C ED+N + +SS N S+ S+
Sbjct: 1770 KYWGLRKNVSANRSNEKSENQNINQKDCSEDMN----LNSSSEKSNSFSSYFSSNETENC 1603
Query: 316 MDNVSPSKNFTPCFENQVCNTK 337
D K C+ N +C T+
Sbjct: 1602 TDLHKMKKR---CYRNDLCETE 1546
>NP565645 NP565645|AY115840.1|AAM55301.1 auxin efflux carrier protein
[Medicago truncatula]
Length = 1575
Score = 28.1 bits (61), Expect = 9.9
Identities = 40/187 (21%), Positives = 76/187 (40%), Gaps = 2/187 (1%)
Frame = +1
Query: 345 VDCEAALPPSKRLHRALEAMSANAAEEGQAHVESSASRMTSIATCCISSIKTSPDVAIND 404
++ EA + +LH + +A+ Q+H AS +T+ I S+++S +
Sbjct: 574 LETEAVIKEDGKLHVIVRRSNASRRSHSQSHTTPRASNLTNAE---IYSLQSSRNPTPRG 744
Query: 405 HEGGGLELQKFDACGGGDSSHIIVHSISANSNPMISTENKLSNQVDEPSTRFQPQETGKN 464
+L GG +S+ + + +N +S + KN
Sbjct: 745 SSFNHTDLHSM--MGGSRNSNFGAFDLKGTPPRTCNYDNSMSTTKGNYPAPNPEMFSPKN 918
Query: 465 VLQCAADQIEELSDFVVSHTANV--DLKTQVHGETYPDLDSKCNEAESNQDSPALSLPPN 522
V+ AA + E+L F S + ++ D+K V E D E + ++ A ++PP
Sbjct: 919 VV--AAKKTEDLHMFFWSSSDSLVSDVKLNVSPEKVEGED----EHKRGEEGGAKTMPP- 1077
Query: 523 IEANIIT 529
A+I+T
Sbjct: 1078--ASIMT 1092
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.308 0.124 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,372,107
Number of Sequences: 36976
Number of extensions: 231685
Number of successful extensions: 1019
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1015
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1019
length of query: 592
length of database: 9,014,727
effective HSP length: 102
effective length of query: 490
effective length of database: 5,243,175
effective search space: 2569155750
effective search space used: 2569155750
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146865.13