BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146862.7 - phase: 0
(198 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77874 similar to GP|6498433|dbj|BAA87836.1 hypothetical protei... 433 e-122
TC88048 similar to GP|20302596|dbj|BAB91125. Ser/Thr kinase {Ara... 31 0.27
TC79991 similar to GP|10177307|dbj|BAB10568. contains similarity... 30 0.61
TC83510 similar to GP|15148393|gb|AAK84777.1 gamma-gliadin {Trit... 30 0.80
TC84479 ENBP1 30 0.80
AL375206 29 1.0
TC86145 homologue to GP|10334499|emb|CAC10211. hypothetical prot... 29 1.0
TC86146 homologue to GP|10334499|emb|CAC10211. hypothetical prot... 29 1.0
AW685919 homologue to PIR|S25298|S252 extensin (clone Tom J-10) ... 29 1.4
TC76563 homologue to PIR|C29356|C29356 hydroxyproline-rich glyco... 29 1.4
TC89300 similar to GP|10176904|dbj|BAB10097. emb|CAB72147.1~gene... 29 1.4
TC79833 similar to PIR|S56678|S56678 aspartate transaminase (EC ... 29 1.4
TC82229 similar to GP|14532838|gb|AAK64101.1 unknown protein {Ar... 29 1.4
BF637188 similar to GP|14334908|gb putative membrane transporter... 28 1.8
BQ143960 weakly similar to SP|P21997|SSGP_ Sulfated surface glyc... 28 3.0
TC80640 27 4.0
TC77287 SP|Q02735|CAPP_MEDSA Phosphoenolpyruvate carboxylase (EC... 27 4.0
TC85618 weakly similar to PIR|T11578|T11578 probable lipoxygenas... 27 4.0
AL367111 homologue to GP|19550437|gb| carotenoid isomerase {Lyco... 27 6.8
BF642076 homologue to GP|23505128|em hypothetical protein {Plasm... 26 8.8
>TC77874 similar to GP|6498433|dbj|BAA87836.1 hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (42%)
Length = 1747
Score = 433 bits (1113), Expect = e-122
Identities = 197/197 (100%), Positives = 197/197 (100%)
Frame = +3
Query: 1 MQITCEASLKCLQGKGPPFTFQCNGSSMEVFPELNNEPGNHPSGNVPEPNHRLGSEFLEP 60
MQITCEASLKCLQGKGPPFTFQCNGSSMEVFPELNNEPGNHPSGNVPEPNHRLGSEFLEP
Sbjct: 111 MQITCEASLKCLQGKGPPFTFQCNGSSMEVFPELNNEPGNHPSGNVPEPNHRLGSEFLEP 290
Query: 61 SNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHPVENQFQYAPINMVSQGY 120
SNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHPVENQFQYAPINMVSQGY
Sbjct: 291 SNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHPVENQFQYAPINMVSQGY 470
Query: 121 PREQYQEFQNFVVIDFEATCDKDKNPHPQEIIEFPSVIVSSVTGQLEACFQTYVRPTCNQ 180
PREQYQEFQNFVVIDFEATCDKDKNPHPQEIIEFPSVIVSSVTGQLEACFQTYVRPTCNQ
Sbjct: 471 PREQYQEFQNFVVIDFEATCDKDKNPHPQEIIEFPSVIVSSVTGQLEACFQTYVRPTCNQ 650
Query: 181 HLSDFCKDLTGIQQIQV 197
HLSDFCKDLTGIQQIQV
Sbjct: 651 HLSDFCKDLTGIQQIQV 701
>TC88048 similar to GP|20302596|dbj|BAB91125. Ser/Thr kinase {Arabidopsis
thaliana}, partial (61%)
Length = 2349
Score = 31.2 bits (69), Expect = 0.27
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = +1
Query: 46 VPEPNHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHPVE 105
+ +P + L + SNE+ N Y D C++HP + C + FE+H P E
Sbjct: 925 IRQPLYDLHRSYSNFSNEYSNGFGYEGD------CYYHPVDNEPCGIELFEHHDDEEPSE 1086
Query: 106 N 106
+
Sbjct: 1087H 1089
>TC79991 similar to GP|10177307|dbj|BAB10568. contains similarity to zinc
finger protein~gene_id:MDC12.23 {Arabidopsis thaliana},
partial (8%)
Length = 1023
Score = 30.0 bits (66), Expect = 0.61
Identities = 15/62 (24%), Positives = 25/62 (40%)
Frame = -3
Query: 41 HPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFY 100
H ++P +H P + HN +HH Y H H + Q ++ N+ Y
Sbjct: 844 HHCFSLPSHHHSHTHHHNHPHIQHHNHNQHHHHYRHHNPNHQHLYHHQVLHSHSHSNY*Y 665
Query: 101 PH 102
P+
Sbjct: 664 PN 659
>TC83510 similar to GP|15148393|gb|AAK84777.1 gamma-gliadin {Triticum
aestivum}, partial (8%)
Length = 971
Score = 29.6 bits (65), Expect = 0.80
Identities = 22/75 (29%), Positives = 27/75 (35%), Gaps = 17/75 (22%)
Frame = +3
Query: 64 FHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFY----------------PHPVE-N 106
FHN P H T CH +P N F NH Y P P+ N
Sbjct: 387 FHNNPCMHQGSWVKTKCHNNP-------SNRFSNHLYFKRSNRVFPPTELQQRPSPISGN 545
Query: 107 QFQYAPINMVSQGYP 121
Q+ P + S+G P
Sbjct: 546 PSQFHPGSSGSRGRP 590
>TC84479 ENBP1
Length = 5108
Score = 29.6 bits (65), Expect = 0.80
Identities = 21/69 (30%), Positives = 31/69 (44%), Gaps = 1/69 (1%)
Frame = -2
Query: 62 NEFHNKPTYHHDYSTWTACHFHPHKMQ-QCQMNAFENHFYPHPVENQFQYAPINMVSQGY 120
N FH HH + HFH H Q Q + F+ H + HP+ A +N Y
Sbjct: 556 NHFHK----HHQH------HFHDHLYQHQKHHHHFQIHLH-HPI------ADLNQQPYSY 428
Query: 121 PREQYQEFQ 129
P++ +Q+ Q
Sbjct: 427 PQQTHQQIQ 401
>AL375206
Length = 451
Score = 29.3 bits (64), Expect = 1.0
Identities = 17/46 (36%), Positives = 23/46 (49%)
Frame = -3
Query: 43 SGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQ 88
S N P +HR + PS+ FH P + +ST T+ H PH Q
Sbjct: 257 SANPPFSHHRHKT----PSS-FHRPPPHSSHFSTHTSSHLQPHIQQ 135
>TC86145 homologue to GP|10334499|emb|CAC10211. hypothetical protein {Cicer
arietinum}, partial (91%)
Length = 1311
Score = 29.3 bits (64), Expect = 1.0
Identities = 13/38 (34%), Positives = 16/38 (41%)
Frame = -3
Query: 65 HNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPH 102
HN P H H HPH QC+ +NH + H
Sbjct: 529 HNPPRCHR-------LHHHPHHPHQCRHVDHQNHLHHH 437
>TC86146 homologue to GP|10334499|emb|CAC10211. hypothetical protein {Cicer
arietinum}, partial (95%)
Length = 1084
Score = 29.3 bits (64), Expect = 1.0
Identities = 13/38 (34%), Positives = 16/38 (41%)
Frame = -2
Query: 65 HNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPH 102
HN P H H HPH QC+ +NH + H
Sbjct: 348 HNPPRCHR-------LHHHPHHPHQCRHVDHQNHLHHH 256
>AW685919 homologue to PIR|S25298|S252 extensin (clone Tom J-10) - tomato,
partial (23%)
Length = 663
Score = 28.9 bits (63), Expect = 1.4
Identities = 14/44 (31%), Positives = 19/44 (42%)
Frame = +2
Query: 40 NHPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFH 83
+H + N+P P HRL + H P +HH T H H
Sbjct: 53 HHTTTNLPHPLHRLLHHHTTINLPHHRHPLHHHLTITSLLPHLH 184
>TC76563 homologue to PIR|C29356|C29356 hydroxyproline-rich glycoprotein
(clone Hyp2.13) - kidney bean (fragment), partial (34%)
Length = 507
Score = 28.9 bits (63), Expect = 1.4
Identities = 14/44 (31%), Positives = 19/44 (42%)
Frame = +3
Query: 40 NHPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFH 83
+H + N+P P HRL + H P +HH T H H
Sbjct: 282 HHTTTNLPHPLHRLLHHHTTINLPHHRHPLHHHLTITSLLPHLH 413
>TC89300 similar to GP|10176904|dbj|BAB10097.
emb|CAB72147.1~gene_id:MAE1.1~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (29%)
Length = 974
Score = 28.9 bits (63), Expect = 1.4
Identities = 15/58 (25%), Positives = 22/58 (37%)
Frame = -2
Query: 50 NHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHPVENQ 107
NH L + ++ T H S PH++ C H YPH V++Q
Sbjct: 889 NHHLSNNHFPTHLPLQDRRTLHQT-SLIPRLQIQPHRLHVCHCRH*NPHHYPHLVDHQ 719
>TC79833 similar to PIR|S56678|S56678 aspartate transaminase (EC 2.6.1.1)
precursor - soybean, partial (66%)
Length = 1045
Score = 28.9 bits (63), Expect = 1.4
Identities = 13/46 (28%), Positives = 24/46 (51%)
Frame = +1
Query: 124 QYQEFQNFVVIDFEATCDKDKNPHPQEIIEFPSVIVSSVTGQLEAC 169
Q++ + V +DF + D P P+ I +FP ++ G++ AC
Sbjct: 430 QFRLYLGLVHVDFSPYFNSDFIPTPKSIYQFPPGPITITFGEMLAC 567
>TC82229 similar to GP|14532838|gb|AAK64101.1 unknown protein {Arabidopsis
thaliana}, partial (8%)
Length = 679
Score = 28.9 bits (63), Expect = 1.4
Identities = 21/89 (23%), Positives = 35/89 (38%), Gaps = 10/89 (11%)
Frame = -3
Query: 24 NGSSMEVFPELNNEPGNHPSGNVP-------EPNHRLGSEFLEPSNEFHNKPTYHHDYST 76
+ S F L N P NH + + + ++ F + + H+ P YHH S+
Sbjct: 599 SASYWRYFHLLTNHPLNH*QPHYDFLAWSWNQIDQKVEDHFESRACDSHDHPVYHHQLSS 420
Query: 77 WTACHFHPHKMQQCQMNAFE---NHFYPH 102
H H + C+M + +HF H
Sbjct: 419 LKIDHLHVLCSRLCEMRHQQKGRHHFSVH 333
>BF637188 similar to GP|14334908|gb putative membrane transporter protein
{Arabidopsis thaliana}, partial (19%)
Length = 448
Score = 28.5 bits (62), Expect = 1.8
Identities = 19/59 (32%), Positives = 26/59 (43%)
Frame = -3
Query: 57 FLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHPVENQFQYAPINM 115
FL+ EF YHH HFHP K Q+ + + + + VENQ +P M
Sbjct: 365 FLQQKVEFVPPLLYHH--------HFHPLKEQEMKES*LQFLMPKYNVENQVVLSPPKM 213
>BQ143960 weakly similar to SP|P21997|SSGP_ Sulfated surface glycoprotein 185
(SSG 185). {Volvox carteri}, partial (13%)
Length = 1023
Score = 27.7 bits (60), Expect = 3.0
Identities = 16/55 (29%), Positives = 24/55 (43%)
Frame = +2
Query: 36 NEPGNHPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDYSTWTACHFHPHKMQQC 90
++P H + N P +H +PSN F +H HF PHK+ +C
Sbjct: 425 HKPSTHYTDNTP--SHFSLISHPKPSNNFFVLIQSNHPQPKNPLSHFSPHKLIKC 583
>TC80640
Length = 980
Score = 27.3 bits (59), Expect = 4.0
Identities = 15/57 (26%), Positives = 20/57 (34%), Gaps = 9/57 (15%)
Frame = +2
Query: 61 SNEFHNKPTYHHD---------YSTWTACHFHPHKMQQCQMNAFENHFYPHPVENQF 108
S + + PTYHH +S W C HP + MN + P F
Sbjct: 623 SQDLED*PTYHHQVVCH*NYLTHSPWKLCPMHPPAVNLPSMNLILVMYKPKLARTNF 793
>TC77287 SP|Q02735|CAPP_MEDSA Phosphoenolpyruvate carboxylase (EC 4.1.1.31)
(PEPCASE). [Alfalfa] {Medicago sativa}, partial (33%)
Length = 1124
Score = 27.3 bits (59), Expect = 4.0
Identities = 9/23 (39%), Positives = 12/23 (52%)
Frame = -2
Query: 51 HRLGSEFLEPSNEFHNKPTYHHD 73
H+ G + PS E H P YH +
Sbjct: 787 HQYGMQLESPSGELHQAPAYHQE 719
>TC85618 weakly similar to PIR|T11578|T11578 probable lipoxygenase (EC
1.13.11.12) CPRD46 drought-inducible - cowpea, partial
(29%)
Length = 1257
Score = 27.3 bits (59), Expect = 4.0
Identities = 20/78 (25%), Positives = 27/78 (33%), Gaps = 8/78 (10%)
Frame = -1
Query: 68 PTYHHDYSTWTACHFHPHKMQ--------QCQMNAFENHFYPHPVENQFQYAPINMVSQG 119
P+YH W H Q QC + F FY H
Sbjct: 714 PSYHQGCHAWNEPMIHKKNSQDQ*ESHQEQCSL*TFP--FYAH----------------- 592
Query: 120 YPREQYQEFQNFVVIDFE 137
+P+E +Q QNFVV+ +
Sbjct: 591 FPQELHQYLQNFVVLQIQ 538
>AL367111 homologue to GP|19550437|gb| carotenoid isomerase {Lycopersicon
esculentum}, partial (4%)
Length = 293
Score = 26.6 bits (57), Expect = 6.8
Identities = 15/49 (30%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
Frame = -2
Query: 38 PGNHPSGNVPEPNHRLGSEFLEPSNEFHNKPTYHHDY-STWTACHFHPH 85
P N P+ P PN+ ++P + + PT+HH + HFH H
Sbjct: 262 PSNQPTN--PRPNNXC----IKPFTKITSLPTFHHFFLQLHHPFHFHHH 134
>BF642076 homologue to GP|23505128|em hypothetical protein {Plasmodium
falciparum 3D7}, partial (0%)
Length = 503
Score = 26.2 bits (56), Expect = 8.8
Identities = 11/40 (27%), Positives = 19/40 (47%)
Frame = -3
Query: 65 HNKPTYHHDYSTWTACHFHPHKMQQCQMNAFENHFYPHPV 104
H+ HD+ + H H H Q ++F+ H P+P+
Sbjct: 288 HHDDXDXHDHEQHSQ-HLHHHHQDHLQHHSFQLHLSPNPL 172
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.135 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,869,640
Number of Sequences: 36976
Number of extensions: 168390
Number of successful extensions: 977
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 957
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 975
length of query: 198
length of database: 9,014,727
effective HSP length: 91
effective length of query: 107
effective length of database: 5,649,911
effective search space: 604540477
effective search space used: 604540477
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146862.7