BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146819.5 + phase: 0 /partial
(599 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80690 weakly similar to PIR|F84587|F84587 hypothetical protein... 180 1e-45
TC87201 similar to PIR|F86388|F86388 hypothetical protein AAG292... 32 0.91
TC89658 weakly similar to PIR|A84527|A84527 hypothetical protein... 31 1.2
BG648786 weakly similar to SP|P10495|GRP1_ Glycine-rich cell wal... 30 2.0
TC79378 similar to PIR|T00518|T00518 hypothetical protein At2g23... 30 2.6
TC90003 weakly similar to GP|19310489|gb|AAL84978.1 AT4g39730/T1... 30 3.4
BQ124448 weakly similar to GP|9279699|dbj| emb|CAB10253.1~gene_i... 29 4.5
TC87039 similar to GP|7269704|emb|CAB79652.1 predicted protein {... 29 4.5
TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-inf... 29 5.9
TC90065 similar to GP|13938209|gb|AAH07225.1 Unknown (protein fo... 28 7.7
TC89780 28 7.7
AW686285 weakly similar to GP|16551831|dbj unnamed protein produ... 28 7.7
TC78692 similar to GP|13384384|gb|AAK21352.1 putative extensin {... 28 7.7
BG584281 weakly similar to GP|14209590|dbj hypothetical protein~... 25 8.4
>TC80690 weakly similar to PIR|F84587|F84587 hypothetical protein At2g20310
[imported] - Arabidopsis thaliana, partial (21%)
Length = 1269
Score = 180 bits (457), Expect = 1e-45
Identities = 94/187 (50%), Positives = 120/187 (63%), Gaps = 15/187 (8%)
Frame = +3
Query: 1 IACRDYPHSRHLCGNFPYSSTPHERHCGQCHCYVCDSPAPCLKWGDGLLNTDHCHATDKS 60
+ACRDYPH+RHLC FP+SSTPHERHCGQCHCYVCDS APCLKWG G+L++DHCHA DK+
Sbjct: 627 VACRDYPHARHLCATFPFSSTPHERHCGQCHCYVCDSLAPCLKWGTGILSSDHCHANDKT 806
Query: 61 DIWKTLRQD-------SLSASTKSG---NVVNSQPNHMLPLETARLSPNSVLVNHTSRST 110
++WK R+D L AS G +V SQ N + P + +LSP SVL ++RST
Sbjct: 807 EVWKIKRKDFKRSLSSPLPASINYGTSLGMVRSQSNEIFPRDIVQLSPISVLRTQSARST 986
Query: 111 ALLPLSANLIPQNQAYWNACS----SLSSRLQNQVSRSTETHPHSV-NLIPQNQAYLPIA 165
A+ S N IPQNQ + S++S +QNQ++R TH V NL N A
Sbjct: 987 AMHTPSLNCIPQNQVSRPKATYSHISVNSGMQNQITRPINTHVSKVPNLTIPNGANHGRY 1166
Query: 166 MNARSSL 172
+ +RS+L
Sbjct: 1167LESRSAL 1187
Score = 70.5 bits (171), Expect(2) = 2e-14
Identities = 42/95 (44%), Positives = 55/95 (57%)
Frame = +3
Query: 291 SGLQNQASRSTATHPQSVNLIPQNQASQPITINAMPLQNYRLQNQISRPNNAPGCSTASN 350
S L+ Q++RSTA H S+N IPQNQ S+P + N +QNQI+RP N S N
Sbjct: 954 SVLRTQSARSTAMHTPSLNCIPQNQVSRPKATYSHISVNSGMQNQITRPINT-HVSKVPN 1130
Query: 351 FTIPNGANNGICQESGPTLARNRYPSNTAARMPLG 385
TIPNGAN+G ES L+R+R P + + G
Sbjct: 1131 LTIPNGANHGRYLESRSALSRDRNPPSLCPKAVTG 1235
Score = 48.9 bits (115), Expect = 5e-06
Identities = 33/96 (34%), Positives = 55/96 (56%)
Frame = +3
Query: 213 SGLQNQASRSTETHPHSVNLMPQNQVSRQITMNACSSLGSGLQNQASRSTATHPHSVNLM 272
S L+ Q++RST H S+N +PQNQVSR + S+ SG+QNQ +R TH V+ +
Sbjct: 954 SVLRTQSARSTAMHTPSLNCIPQNQVSRPKATYSHISVNSGMQNQITRPINTH---VSKV 1124
Query: 273 PQNQVSRQITMNACSSLGSGLQNQASRSTATHPQSV 308
P +T+ ++ G L+++++ S +P S+
Sbjct: 1125 P------NLTIPNGANHGRYLESRSALSRDRNPPSL 1214
Score = 26.6 bits (57), Expect(2) = 2e-14
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +1
Query: 377 NTAARMPLGVRNHAIQKKRG 396
++ + LGVR+HAIQ++RG
Sbjct: 1210 HSVPKQSLGVRSHAIQRERG 1269
>TC87201 similar to PIR|F86388|F86388 hypothetical protein AAG29221.1
[imported] - Arabidopsis thaliana, partial (41%)
Length = 991
Score = 31.6 bits (70), Expect = 0.91
Identities = 29/101 (28%), Positives = 48/101 (46%), Gaps = 2/101 (1%)
Frame = +3
Query: 85 PNHMLPLETARLSPNS-VLVNHTSRSTALLPLSANLIPQNQAYWNACSSLSSRLQNQVSR 143
P LP+ ++ ++ +S NH +RST LP+S + I + Y + SS +
Sbjct: 30 PTLRLPVSSSTITTSSYTYTNHHARSTLCLPISPSTIASSSIYLQ-ITPTSSICV*VTTS 206
Query: 144 STETHPHSVNLIPQNQAYLPIAMNARSSL-GSGLQLQVSRS 183
ST P S + I + YL +A + + + + LQVS S
Sbjct: 207 STLCLPISSSTITTSSIYLQVATSPTICVHPTSICLQVSSS 329
>TC89658 weakly similar to PIR|A84527|A84527 hypothetical protein At2g15280
[imported] - Arabidopsis thaliana, partial (59%)
Length = 1217
Score = 31.2 bits (69), Expect = 1.2
Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Frame = -2
Query: 176 LQLQVSRSAETHPLSVNLM---PQNQASRQITMNAYSSLGSGLQNQASRSTETHPHSVNL 232
+Q+ +SR P S NL+ P+ + S I N+Y + +G+ ++ S H SVNL
Sbjct: 1045 VQVLMSRVLSFPPSSGNLIQSEPKTRVSISIYQNSYKASSNGVH*KSQESNHLHQSSVNL 866
>BG648786 weakly similar to SP|P10495|GRP1_ Glycine-rich cell wall structural
protein 1.0 precursor (GRP 1.0). [Kidney bean French
bean], partial (17%)
Length = 668
Score = 30.4 bits (67), Expect = 2.0
Identities = 18/51 (35%), Positives = 25/51 (48%)
Frame = +1
Query: 396 GHGVGTGNPVTMNHVTAPGAPGFSNHINTQYSDRSNAAAATGFSNSRNGYG 446
G VG ++N+ +APG G N N YSDR + + G + GYG
Sbjct: 292 GAAVGARESNSVNYGSAPGGYGGGNSAN--YSDRESNSVNYGSAPGVGGYG 438
>TC79378 similar to PIR|T00518|T00518 hypothetical protein At2g23140
[imported] - Arabidopsis thaliana, partial (36%)
Length = 2133
Score = 30.0 bits (66), Expect = 2.6
Identities = 33/169 (19%), Positives = 74/169 (43%), Gaps = 4/169 (2%)
Frame = +1
Query: 201 RQITMNAYSSLGSGLQNQASRSTETHPHSVNLMPQNQVSRQITMNACSSLGSGLQN---- 256
+ T+N S L +++ +R + PHS + +P + S + + +S G+ + +
Sbjct: 1069 KSTTLNQASILHGYMESGTTRESPVFPHSRSNLPSSPESAR--SRSFNSPGNNIPSSGGI 1242
Query: 257 QASRSTATHPHSVNLMPQNQVSRQITMNACSSLGSGLQNQASRSTATHPQSVNLIPQNQA 316
Q ++ HP S++ + + M+A + +G+ + RS ++ S++ + Q
Sbjct: 1243 QREGTSPLHPRSISKRSLSGIVNGQYMDAARTSPTGIDD---RSASSDESSIDSMGQPSI 1413
Query: 317 SQPITINAMPLQNYRLQNQISRPNNAPGCSTASNFTIPNGANNGICQES 365
S ++ + QN + R + G + +NF G ++ Q S
Sbjct: 1414 SPSRRESSGAFSSELSQNHV-RAVSDSGAFSDANFQETQGDDSNASQLS 1557
>TC90003 weakly similar to GP|19310489|gb|AAL84978.1 AT4g39730/T19P19_120
{Arabidopsis thaliana}, partial (75%)
Length = 749
Score = 29.6 bits (65), Expect = 3.4
Identities = 18/59 (30%), Positives = 26/59 (43%), Gaps = 8/59 (13%)
Frame = -2
Query: 204 TMNAYSSLGSGLQNQASRSTETH--------PHSVNLMPQNQVSRQITMNACSSLGSGL 254
T+ S+ SG+QN+ T TH H+ + + S MN C+ GSGL
Sbjct: 691 TLKPESNTNSGIQNKKG*LTNTHNNRLKSYLTHNWGIQNRKLRSNFAMMNGCAGFGSGL 515
Score = 28.5 bits (62), Expect = 7.7
Identities = 20/74 (27%), Positives = 29/74 (39%), Gaps = 8/74 (10%)
Frame = -2
Query: 243 TMNACSSLGSGLQNQASRSTATH--------PHSVNLMPQNQVSRQITMNACSSLGSGLQ 294
T+ S+ SG+QN+ T TH H+ + + S MN C+ GSGL
Sbjct: 691 TLKPESNTNSGIQNKKG*LTNTHNNRLKSYLTHNWGIQNRKLRSNFAMMNGCAGFGSGLI 512
Query: 295 NQASRSTATHPQSV 308
PQ +
Sbjct: 511 QLIMTKIPHRPQLI 470
>BQ124448 weakly similar to GP|9279699|dbj|
emb|CAB10253.1~gene_id:MWI23.18~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (6%)
Length = 627
Score = 29.3 bits (64), Expect = 4.5
Identities = 15/42 (35%), Positives = 22/42 (51%)
Frame = -3
Query: 121 PQNQAYWNACSSLSSRLQNQVSRSTETHPHSVNLIPQNQAYL 162
PQ A S+L S+ + S ++H H VN +P +AYL
Sbjct: 256 PQRNGKILASSAL*SKAGFPIQSSDDSHRHPVNGVPSKRAYL 131
>TC87039 similar to GP|7269704|emb|CAB79652.1 predicted protein {Arabidopsis
thaliana}, partial (57%)
Length = 1150
Score = 29.3 bits (64), Expect = 4.5
Identities = 13/35 (37%), Positives = 15/35 (42%), Gaps = 2/35 (5%)
Frame = -1
Query: 1 IACRDYPHSRHLCGNF--PYSSTPHERHCGQCHCY 33
+ C Y C N + S PHE HC HCY
Sbjct: 880 VECFPYYDQHAYCHNLYNKHCSIPHELHCWGLHCY 776
>TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-infected
erythrocyte surface antigen {Plasmodium falciparum},
partial (2%)
Length = 2007
Score = 28.9 bits (63), Expect = 5.9
Identities = 35/173 (20%), Positives = 65/173 (37%), Gaps = 5/173 (2%)
Frame = +1
Query: 425 QYSDRSNAAAATGFSNSRNGYGQDGIRITNAINPLLTQPSHSLAYETQPSYQSNSIQNLY 484
Q + + +++ F N + + T+ + + S A S Q+++ +
Sbjct: 1150 QEGTQESGSSSNTFDNKDAASNKVQLTTTSDTSSEQKKDESSSAESKSESSQNDNANS-- 1323
Query: 485 GYNNFQGDESLSSYVARLNTNRYLNEHQIGSQNENIINSGATVQDVFQQNPHGGIQNEGF 544
G +N DES N N+ + Q+ + +EN + ++ +N + NE
Sbjct: 1324 GQSNTTSDESA-------NDNK--DSSQVTTSSENSAEGNSNTENNSDENQNDSKNNEN- 1473
Query: 545 LARDSSGAENT-----NQNISYVQNLVSQNISQNANESSTPFNGNTQLSLDDI 592
DS N NQN + Q S+N NES N + + D+
Sbjct: 1474 -TNDSGNTSNDANVNENQNENAAQTKTSENEGDAQNESVESKKENNESAHKDV 1629
>TC90065 similar to GP|13938209|gb|AAH07225.1 Unknown (protein for
IMAGE:3161441) {Homo sapiens}, partial (10%)
Length = 656
Score = 28.5 bits (62), Expect = 7.7
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +1
Query: 189 LSVNLMPQNQASRQITMNAYSSLGSGLQNQASRSTETHPHSV 230
LS NL+ + Y +L SGL N++S+ T + H+V
Sbjct: 319 LSPNLLRNKSVYEGVLQRGYRTLNSGLCNRSSKVTGNYFHNV 444
>TC89780
Length = 1402
Score = 28.5 bits (62), Expect = 7.7
Identities = 32/113 (28%), Positives = 47/113 (41%), Gaps = 6/113 (5%)
Frame = +1
Query: 477 SNSIQNLYGYNNFQGDESL--SSYVARLNTNRYLNEHQIGSQNENIINSGATVQDVFQQN 534
S S + GYNN Q S S+ + + ++ LN G+QNEN +N +
Sbjct: 334 SKSYDDTRGYNN-QNRTSFRTSNLLGQEKSHSPLN----GNQNENSVNCQYQSSETKHHG 498
Query: 535 PHGGIQNEGFLARDSSGAENTNQNISYVQNLVSQN----ISQNANESSTPFNG 583
PHG G+ + S + T+ QN S+N + E S FNG
Sbjct: 499 PHG----RGYPDSEPSHSFPTDSQDGLHQNDASKNKNDLYALVFREQSPEFNG 645
>AW686285 weakly similar to GP|16551831|dbj unnamed protein product {Homo
sapiens}, partial (8%)
Length = 666
Score = 28.5 bits (62), Expect = 7.7
Identities = 29/100 (29%), Positives = 39/100 (39%), Gaps = 12/100 (12%)
Frame = +3
Query: 493 ESLSSYVARLNTNRYLNEHQIGSQNENIINSGATVQDVFQQNPHG-GIQNEGFLARD--- 548
E + + L + Y N H+ Q + N Q Q P G N+G + +
Sbjct: 48 EDVLQKIQHLYSYNYNNSHKFSQQRGSNYNQ----QVEKSQFPQGINSANQGAVGKPLVA 215
Query: 549 SSGAENTNQNISYVQNLVSQNISQN--------ANESSTP 580
SG Q + Q VSQN SQN AN +STP
Sbjct: 216 ESGNGQQQQPVQQSQQQVSQNQSQNLANGQPNQANRTSTP 335
>TC78692 similar to GP|13384384|gb|AAK21352.1 putative extensin {Oryza
sativa (japonica cultivar-group)}, partial (21%)
Length = 1264
Score = 28.5 bits (62), Expect = 7.7
Identities = 32/113 (28%), Positives = 44/113 (38%), Gaps = 13/113 (11%)
Frame = +3
Query: 284 NACSSLGSGLQNQASRSTATHPQSVNLIPQNQASQPITINAMPLQNYRLQNQISRPNNAP 343
NA + S L+ A + ++ + N N + ITI A L NYRL+ ++S N
Sbjct: 39 NAAMAAWSSLKQLAKETASSSTEPEN----NDELEQITI-ARALLNYRLKEKMSMSNPNA 203
Query: 344 GCSTASNFTIPNGANNG-------------ICQESGPTLARNRYPSNTAARMP 383
F I N ICQ++GP RNR S T P
Sbjct: 204 RIPFQKKFQIQNIRPTSSQSPATTSKILPLICQKTGP---RNRPSSATPNENP 353
>BG584281 weakly similar to GP|14209590|dbj hypothetical protein~similar to
Arabidopsis thaliana chromosome 3 MMM17.14, partial
(28%)
Length = 761
Score = 25.0 bits (53), Expect(2) = 8.4
Identities = 17/48 (35%), Positives = 22/48 (45%)
Frame = +2
Query: 79 NVVNSQPNHMLPLETARLSPNSVLVNHTSRSTALLPLSANLIPQNQAY 126
N +S P H L S NS+ N SR+T PLSA P + +
Sbjct: 56 NSSSSSPPHYSSLP----SSNSIPPNFISRATTPKPLSATRRPWEEVF 187
Score = 21.6 bits (44), Expect(2) = 8.4
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +1
Query: 148 HPHSVNLIPQN 158
HPH + LIPQ+
Sbjct: 277 HPHPLRLIPQS 309
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.125 0.364
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,067,708
Number of Sequences: 36976
Number of extensions: 316403
Number of successful extensions: 1402
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 1264
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1391
length of query: 599
length of database: 9,014,727
effective HSP length: 102
effective length of query: 497
effective length of database: 5,243,175
effective search space: 2605857975
effective search space used: 2605857975
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146819.5