BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146819.1 + phase: 0
(221 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87682 weakly similar to PIR|T04527|T04527 hypothetical protein... 67 2e-18
TC83706 weakly similar to PIR|T48283|T48283 ankyrin-like protein... 31 0.33
TC78688 weakly similar to PIR|B86326|B86326 hypothetical protein... 31 0.43
TC91435 similar to PIR|T38900|T38900 probable alpha-mannosidase ... 29 1.6
BE202920 29 1.6
TC88173 similar to GP|4512677|gb|AAD21731.1| unknown protein {Ar... 28 3.6
TC77197 similar to GP|15215652|gb|AAK91371.1 AT3g57090/F24I3_170... 28 3.6
TC79124 1-aminocyclopropanecarboxylic acid synthase [Medicago tr... 28 3.6
TC81932 similar to GP|13430778|gb|AAK26011.1 unknown protein {Ar... 28 3.6
TC79773 similar to PIR|C96774|C96774 AtHVA22a 65476-64429 [impo... 27 6.2
AW775133 weakly similar to PIR|T02440|T024 hypothetical protein ... 27 6.2
TC85237 similar to GP|4200165|emb|CAA76145.1 neutral invertase {... 27 8.1
BE205155 weakly similar to PIR|C96774|C967 AtHVA22a 65476-64429... 27 8.1
BM815214 similar to GP|22136992|gb unknown protein {Arabidopsis ... 27 8.1
>TC87682 weakly similar to PIR|T04527|T04527 hypothetical protein F16A16.170
- Arabidopsis thaliana, partial (38%)
Length = 1040
Score = 67.4 bits (163), Expect(3) = 2e-18
Identities = 33/87 (37%), Positives = 55/87 (62%)
Frame = +2
Query: 40 MMLKWLPLWIVDKLLLILTWFILGDMEKYGIKRPSMGPLQLKNTVGKTPVLDIGALEKIR 99
++L +L V+KL++I + + GD+ KYGI PS GP +K GK PV+D+G ++KI+
Sbjct: 683 VLLNYLSPSTVEKLVVIASRIVYGDLSKYGIPFPSEGPFTMKMKYGKFPVIDVGTVKKIK 862
Query: 100 SGDINVVPGIKRINKNGEVELVNGEKL 126
SG+I V+P E+E ++G ++
Sbjct: 863 SGEIQVLP--------AEIESISGNQV 919
Score = 38.9 bits (89), Expect(3) = 2e-18
Identities = 20/41 (48%), Positives = 30/41 (72%), Gaps = 2/41 (4%)
Frame = +1
Query: 1 MELSLDLSNHHALPSMVVRSSVHVLPREI--FGISTFELAV 39
ME++LDL+N A PS++VRS VH+L R++ +G EL+V
Sbjct: 580 MEIALDLANFGAKPSIIVRSPVHILSRDMMYYGGGFVELSV 702
Score = 21.9 bits (45), Expect(3) = 2e-18
Identities = 8/30 (26%), Positives = 18/30 (59%), Gaps = 1/30 (3%)
Frame = +1
Query: 129 DAVVLATGYRSNVPSWLQEGEFF-SKNGYP 157
D+++ TG++ + WL F +++G+P
Sbjct: 949 DSIIFCTGFQRSTQKWL*GWVIF*NEDGFP 1038
>TC83706 weakly similar to PIR|T48283|T48283 ankyrin-like protein -
Arabidopsis thaliana, partial (34%)
Length = 746
Score = 31.2 bits (69), Expect = 0.33
Identities = 17/74 (22%), Positives = 39/74 (51%)
Frame = +1
Query: 12 ALPSMVVRSSVHVLPREIFGISTFELAVMMLKWLPLWIVDKLLLILTWFILGDMEKYGIK 71
+L ++V++SV V+ RE T + L W+ ++ L +++ ++GD ++ I
Sbjct: 286 SLAVVIVQTSVVVIEREAKKQMT--AVINKLMWIACVLISVAFLAMSYIVVGDQKELAIA 459
Query: 72 RPSMGPLQLKNTVG 85
++G + + T+G
Sbjct: 460 ATALGTVIMAATLG 501
>TC78688 weakly similar to PIR|B86326|B86326 hypothetical protein AAF82235.1
[imported] - Arabidopsis thaliana, partial (63%)
Length = 1294
Score = 30.8 bits (68), Expect = 0.43
Identities = 49/207 (23%), Positives = 80/207 (37%), Gaps = 26/207 (12%)
Frame = +3
Query: 41 MLKWLPLWIVDKLLLILTWFILG------DMEKYGIKRPSMGPLQLKNTVGKTPVLDIGA 94
+LK L ++ + L+++ FI +EKYG+K P + + +
Sbjct: 330 LLKSLLCLLLSPMRLVISKFIESYLLWKLPLEKYGLKPEH--PFEEDFASCQIAITPESF 503
Query: 95 LEKIRSGDINVVPGIKRINKNGEVELVNGEKLDIDAVVLATGY--RSNVPSWLQEGEFFS 152
+ G I K NG +E + K+D D V+LATGY + + + L E F S
Sbjct: 504 FNEADKGKIIFKRASKWCFWNGGIEFDDNTKIDADVVLLATGYDGKKKLKTILPE-PFSS 680
Query: 153 KNGYPK--MPFPHGW--------------KGNSGLYAVGFTKRGLSGASSDAVKI--AQD 194
YP MP G + S LY L+G + K+ A+
Sbjct: 681 LLEYPTGIMPLYRGTVHPSIPNMAFVGYVESVSNLYTSEIRSMWLAGLIDNKFKLPSAEK 860
Query: 195 IGKVWKQETKQKKQRTTACHRRCISQF 221
+ ++ + K+ T R CI+ F
Sbjct: 861 MLSQTIKDMETMKKSTRFYKRNCITTF 941
>TC91435 similar to PIR|T38900|T38900 probable alpha-mannosidase - fission
yeast (Schizosaccharomyces pombe), partial (3%)
Length = 756
Score = 28.9 bits (63), Expect = 1.6
Identities = 23/75 (30%), Positives = 36/75 (47%)
Frame = -1
Query: 78 LQLKNTVGKTPVLDIGALEKIRSGDINVVPGIKRINKNGEVELVNGEKLDIDAVVLATGY 137
L ++N V + VL + A+ + D+ G NGE+ +VN LD+ +A Y
Sbjct: 567 LIVENPVARVIVLQLQAIRR----DLEWPQG------NGELAIVNLNFLDVVLEQVALEY 418
Query: 138 RSNVPSWLQEGEFFS 152
NVPS L++ S
Sbjct: 417 ALNVPSRLEDAPALS 373
>BE202920
Length = 465
Score = 28.9 bits (63), Expect = 1.6
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = -1
Query: 203 TKQKKQRTTACHRRC 217
TKQ +QR+TAC RRC
Sbjct: 222 TKQTRQRSTACDRRC 178
>TC88173 similar to GP|4512677|gb|AAD21731.1| unknown protein {Arabidopsis
thaliana}, partial (19%)
Length = 898
Score = 27.7 bits (60), Expect = 3.6
Identities = 11/29 (37%), Positives = 21/29 (71%)
Frame = +1
Query: 103 INVVPGIKRINKNGEVELVNGEKLDIDAV 131
++++PG++R+ KNGE E V+ D+ A+
Sbjct: 526 VSIIPGLQRLGKNGEEEDVS----DVSAI 600
>TC77197 similar to GP|15215652|gb|AAK91371.1 AT3g57090/F24I3_170
{Arabidopsis thaliana}, partial (68%)
Length = 1034
Score = 27.7 bits (60), Expect = 3.6
Identities = 25/109 (22%), Positives = 44/109 (39%), Gaps = 1/109 (0%)
Frame = +3
Query: 100 SGDINVVPGIKRINKNGEVELVNGEKLDIDAVVLATGY-RSNVPSWLQEGEFFSKNGYPK 158
S D +++ G +R E + + E + + L+ S P +Q G ++ P
Sbjct: 138 SCDPDIIAGCEREVAEAEKQPASDEAMQESLLRLSWALVHSKRPEDVQRGIAMLQSSLPS 317
Query: 159 MPFPHGWKGNSGLYAVGFTKRGLSGASSDAVKIAQDIGKVWKQETKQKK 207
P + L AVG+ K G S + V+ +I W+Q K+
Sbjct: 318 TVDPLKQREKLYLLAVGYYKSGDYSRSRELVEKCLEIASDWRQALSLKE 464
>TC79124 1-aminocyclopropanecarboxylic acid synthase [Medicago truncatula]
Length = 1862
Score = 27.7 bits (60), Expect = 3.6
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +1
Query: 32 ISTFELAVMMLKWLPLWIVDKLLLILTWFILG 63
I T + +LK L W++ ++ L+L WF +G
Sbjct: 277 IKTLFIQPKILKVLSKWVLQRISLLLIWFKIG 372
>TC81932 similar to GP|13430778|gb|AAK26011.1 unknown protein {Arabidopsis
thaliana}, partial (18%)
Length = 1139
Score = 27.7 bits (60), Expect = 3.6
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -3
Query: 177 TKRGLSGASSDAVKIAQDIGKVWKQETKQKKQRTTACHRRC 217
T+ +S ASS + A + + + + QR CHRRC
Sbjct: 486 TRLSVSSASSASNSTASALSPPLHHQLQHRLQRRVFCHRRC 364
>TC79773 similar to PIR|C96774|C96774 AtHVA22a 65476-64429 [imported] -
Arabidopsis thaliana, partial (88%)
Length = 928
Score = 26.9 bits (58), Expect = 6.2
Identities = 13/32 (40%), Positives = 20/32 (61%), Gaps = 1/32 (3%)
Frame = +2
Query: 32 ISTFELA-VMMLKWLPLWIVDKLLLILTWFIL 62
I+ FEL +L+W+P+W KL+L W +L
Sbjct: 278 ITLFELTFAKILEWIPIWPYAKLIL-TCWLVL 370
>AW775133 weakly similar to PIR|T02440|T024 hypothetical protein At2g41450
[imported] - Arabidopsis thaliana, partial (5%)
Length = 604
Score = 26.9 bits (58), Expect = 6.2
Identities = 18/61 (29%), Positives = 32/61 (51%), Gaps = 2/61 (3%)
Frame = -2
Query: 86 KTPVLDIGALEKIRSGDINVVPGIKRINKNGEVELVNGEKLDIDAVVLAT--GYRSNVPS 143
+TP + A+ +RS NV+ G+K++N+ V E+ DI+ ++A G R+
Sbjct: 492 QTPAKTLSAI--VRSAGGNVISGLKKVNEASTTIFVTCEE-DIEEAMMAAKKGIRTFDSE 322
Query: 144 W 144
W
Sbjct: 321 W 319
>TC85237 similar to GP|4200165|emb|CAA76145.1 neutral invertase {Daucus
carota}, partial (68%)
Length = 2255
Score = 26.6 bits (57), Expect = 8.1
Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
Frame = +1
Query: 109 IKRINKNGEVELVNGEKLDIDAVVLATGYRSNVPSWL-----QEGEFFSKNGYP 157
IK+IN E+ E+ DAV Y +PSWL +EG +F N P
Sbjct: 1150 IKKIN---EIYRYKTEEYSTDAVNKFNIYPEQIPSWLVDWISEEGGYFIGNLQP 1302
>BE205155 weakly similar to PIR|C96774|C967 AtHVA22a 65476-64429 [imported]
- Arabidopsis thaliana, partial (24%)
Length = 610
Score = 26.6 bits (57), Expect = 8.1
Identities = 15/34 (44%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +3
Query: 32 ISTFELAVM-MLKWLPLWIVDKLLLILTWFILGD 64
I FE A M +L W LW KL++I W I+ D
Sbjct: 153 IYLFEYAFMNLLLWFQLWPYTKLMIIF-WLIIPD 251
>BM815214 similar to GP|22136992|gb unknown protein {Arabidopsis thaliana},
partial (24%)
Length = 596
Score = 26.6 bits (57), Expect = 8.1
Identities = 16/55 (29%), Positives = 30/55 (54%)
Frame = +1
Query: 88 PVLDIGALEKIRSGDINVVPGIKRINKNGEVELVNGEKLDIDAVVLATGYRSNVP 142
P+L LE +R+ + + IK + ++G V +G DA++ TGY+ ++P
Sbjct: 154 PLLKGMKLENVRNICFHTL--IKCVYEDGLVAFEDGFSTYADAIIHCTGYKYHIP 312
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.138 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,259,140
Number of Sequences: 36976
Number of extensions: 96519
Number of successful extensions: 500
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 496
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 500
length of query: 221
length of database: 9,014,727
effective HSP length: 92
effective length of query: 129
effective length of database: 5,612,935
effective search space: 724068615
effective search space used: 724068615
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146819.1