BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146807.10 - phase: 0
(234 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA921522 181 3e-46
AL366606 78 3e-15
BE203370 60 9e-10
TC82298 SP|P03730|VTAI_LAMBD Tail assembly protein I. {Bacteriop... 31 0.47
BQ123351 31 0.47
TC87036 similar to GP|11138330|gb|AAG31326.1 putative serine/thr... 28 2.3
TC87233 similar to PIR|T06048|T06048 kinesin-related protein kat... 28 3.0
TC77879 similar to PIR|D86442|D86442 probable amino acid permeas... 28 4.0
TC77878 similar to PIR|D86442|D86442 probable amino acid permeas... 28 4.0
TC92512 similar to GP|11385586|gb|AAG34873.1 putative chloroplas... 27 6.7
TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1 ... 27 6.7
TC89517 weakly similar to PIR|A85475|A85475 hypothetical protein... 27 8.8
TC81446 weakly similar to GP|15777865|gb|AAL05893.1 At1g54460/F2... 27 8.8
>CA921522
Length = 767
Score = 181 bits (458), Expect = 3e-46
Identities = 89/179 (49%), Positives = 114/179 (62%), Gaps = 2/179 (1%)
Frame = +3
Query: 1 MNAIEIFHSKNPVPTLLADTYHAIHDRTLKGRGYILCCVPLLYRWFISHLPSS--FHDNS 58
+ AI+IF S+NPVPTLL DTY +IH RT GRG ILCC LLYRW SHLP + F N
Sbjct: 186 IKAIQIFLSRNPVPTLLGDTYLSIHRRTQAGRGTILCCAQLLYRWITSHLPRTPRFTTNP 365
Query: 59 ENWSYSQRIMALTPNEVVWITPTAQVKEIITGCGDFLNVPLLGTRGGINYNPELAMRQFG 118
EN +S+R+M+LTP EVVW II CG F NVPLLG GGI+YNP LA QFG
Sbjct: 366 ENLLWSKRLMSLTPAEVVWYDRVYDKGTIIDSCGKFANVPLLGMEGGISYNPTLARHQFG 545
Query: 119 FPMKAKPINLATSPEFFYYSNAPTGQREAFIGAWSKVCRKSVKHLGVRSGIAHEAYTQW 177
+PM+ KP+++ ++ ++ TG RE + AW + R+ LG ++G +YTQW
Sbjct: 546 YPMERKPLSIYLENVYYLNADDSTGMREHVVRAWHTIRRRDKDQLGKKTGAISSSYTQW 722
>AL366606
Length = 393
Score = 78.2 bits (191), Expect = 3e-15
Identities = 41/57 (71%), Positives = 43/57 (74%), Gaps = 6/57 (10%)
Frame = -1
Query: 1 MNAIEIFHSKNPVPTLLADTYHAIHDRTLKGRGYILCCVPLL------YRWFISHLP 51
MNAIE+FHSKNPVPTLLADTYHAIHDRTLKGRGYIL L +R F S LP
Sbjct: 318 MNAIEVFHSKNPVPTLLADTYHAIHDRTLKGRGYILWLHILFSNRVVYFRTFRSSLP 148
>BE203370
Length = 454
Score = 59.7 bits (143), Expect = 9e-10
Identities = 27/41 (65%), Positives = 33/41 (79%)
Frame = +1
Query: 1 MNAIEIFHSKNPVPTLLADTYHAIHDRTLKGRGYILCCVPL 41
++AI++F +KNPVPTLLAD YH+IHDR GRG IL C PL
Sbjct: 331 IHAIQMFLTKNPVPTLLADMYHSIHDRNQVGRGAILGCAPL 453
>TC82298 SP|P03730|VTAI_LAMBD Tail assembly protein I. {Bacteriophage
lambda}, complete
Length = 1004
Score = 30.8 bits (68), Expect = 0.47
Identities = 11/23 (47%), Positives = 18/23 (77%)
Frame = +1
Query: 209 RCNFYARYCGSDSESESEDHPTT 231
RC + AR+CG+D+ +ES++ P T
Sbjct: 652 RCQYGARWCGADAGTESQNSPYT 720
>BQ123351
Length = 725
Score = 30.8 bits (68), Expect = 0.47
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +2
Query: 148 FIGAWSKVCRKSVKHLGVRSGIAHEAYTQW 177
FI AW V ++ LG RS + +E+YT+W
Sbjct: 8 FIQAWRAVHKREKGQLGRRS*LVYESYTKW 97
>TC87036 similar to GP|11138330|gb|AAG31326.1 putative serine/threonine
kinase GDBrPK {Vitis vinifera}, partial (66%)
Length = 1212
Score = 28.5 bits (62), Expect = 2.3
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 45 WFISHLPSSFHDNSENWS 62
WF+ +LP F ++ ENWS
Sbjct: 692 WFLKNLPLEFMEDGENWS 745
>TC87233 similar to PIR|T06048|T06048 kinesin-related protein katB -
Arabidopsis thaliana, partial (18%)
Length = 1488
Score = 28.1 bits (61), Expect = 3.0
Identities = 18/52 (34%), Positives = 30/52 (57%), Gaps = 4/52 (7%)
Frame = +1
Query: 176 QWNLMLKKE----LVKVRRELEEKDELLMRDSKRARGRCNFYARYCGSDSES 223
QWNL+LKKE +K RR+ +K + + S + + R +++ C SD +S
Sbjct: 721 QWNLLLKKERLGLTLKGRRQHYQKISVELNVSLKLQIRR*YHSMTCISDYKS 876
>TC77879 similar to PIR|D86442|D86442 probable amino acid permease
[imported] - Arabidopsis thaliana, partial (81%)
Length = 2037
Score = 27.7 bits (60), Expect = 4.0
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 3/50 (6%)
Frame = -3
Query: 161 KHLGVRSGIAHEAYTQWNLMLKKE---LVKVRRELEEKDELLMRDSKRAR 207
+ +G SG + +WNLMLK+ V+ +R +EE+ E+ + KR +
Sbjct: 196 ERIGRVSGSMR*QFVRWNLMLKENYHGCVQWQRGIEEEKEIKVIKLKRVK 47
>TC77878 similar to PIR|D86442|D86442 probable amino acid permease
[imported] - Arabidopsis thaliana, partial (17%)
Length = 564
Score = 27.7 bits (60), Expect = 4.0
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 3/50 (6%)
Frame = -1
Query: 161 KHLGVRSGIAHEAYTQWNLMLKKE---LVKVRRELEEKDELLMRDSKRAR 207
+ +G SG + +WNLMLK+ V+ +R +EE+ E+ + KR +
Sbjct: 168 ERIGRVSGSMR*QFVRWNLMLKENYHGCVQWQRGIEEEKEIKVIKLKRVK 19
>TC92512 similar to GP|11385586|gb|AAG34873.1 putative chloroplast RNA
helicase VDL isoform 1 {Nicotiana tabacum}, partial
(32%)
Length = 826
Score = 26.9 bits (58), Expect = 6.7
Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 4/52 (7%)
Frame = -2
Query: 163 LGVRSGIAHEAYTQWNLML-KKELVKVRRELEEKDELLMRD---SKRARGRC 210
L + SG+ T+W LML ++E + E EEK+++ D ++R R RC
Sbjct: 246 LAMASGVVVVVVTRWWLMLVEEEEEEEEEEEEEKEKVWF*DEGRTRRGRRRC 91
>TC77925 similar to GP|18700163|gb|AAL77693.1 AT4g02720/T10P11_1
{Arabidopsis thaliana}, partial (47%)
Length = 1538
Score = 26.9 bits (58), Expect = 6.7
Identities = 16/44 (36%), Positives = 23/44 (51%)
Frame = +3
Query: 183 KELVKVRRELEEKDELLMRDSKRARGRCNFYARYCGSDSESESE 226
KE+ + + E +E DS+ + R Y + SDSESESE
Sbjct: 522 KEIKQKAKSESESEESKESDSELRKKRRKSYKKSRESDSESESE 653
>TC89517 weakly similar to PIR|A85475|A85475 hypothetical protein AT4g40080
[imported] - Arabidopsis thaliana, partial (46%)
Length = 1177
Score = 26.6 bits (57), Expect = 8.8
Identities = 19/71 (26%), Positives = 28/71 (38%), Gaps = 1/71 (1%)
Frame = -3
Query: 8 HSKNPVPTLLADTYHAIHDRTLKGRGYILCCVPLLYRWFISHLPSSFHDNSENWSYS-QR 66
H NP+ +H IH Y+ + L W I P+SFH + S+S Q
Sbjct: 719 HHHNPI------IFHQIH--------YLNNHLVLFTHWSIRFFPNSFHQGQQRISFS*QI 582
Query: 67 IMALTPNEVVW 77
+ N +W
Sbjct: 581 TIGKIRNSFLW 549
>TC81446 weakly similar to GP|15777865|gb|AAL05893.1 At1g54460/F20D21_28
{Arabidopsis thaliana}, partial (36%)
Length = 1420
Score = 26.6 bits (57), Expect = 8.8
Identities = 11/32 (34%), Positives = 17/32 (52%)
Frame = +2
Query: 55 HDNSENWSYSQRIMALTPNEVVWITPTAQVKE 86
HD+ +NWS + +A T V PT ++ E
Sbjct: 626 HDDEDNWSVASSSVASTRKVTVGTAPTFRIYE 721
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.136 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,296,711
Number of Sequences: 36976
Number of extensions: 118027
Number of successful extensions: 607
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 606
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 606
length of query: 234
length of database: 9,014,727
effective HSP length: 93
effective length of query: 141
effective length of database: 5,575,959
effective search space: 786210219
effective search space used: 786210219
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146807.10