BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146776.17 - phase: 0
(304 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78009 similar to GP|19424097|gb|AAL87328.1 unknown protein {Ar... 497 e-141
BF632953 similar to GP|19424097|gb| unknown protein {Arabidopsis... 104 4e-23
TC83390 similar to SP|O75533|S3B1_HUMAN Splicing factor 3B subun... 38 0.004
TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 30 1.5
TC79150 similar to GP|18461304|dbj|BAB84499. putative receptor-l... 28 3.3
TC86477 homologue to GP|15408819|dbj|BAB64211. tubulin beta-4 ch... 28 4.4
BF634872 28 4.4
BF637342 similar to SP|Q99334|MT1_CO Metallothionein-like protei... 28 5.7
TC86058 similar to GP|15912261|gb|AAL08264.1 AT4g27720/T29A15_21... 28 5.7
TC90776 similar to GP|7269000|emb|CAB80733.1 contains EST gb:AI9... 28 5.7
BQ144005 27 7.5
TC88968 similar to GP|12329988|emb|CAC24566. trypsin inhibitor {... 27 9.7
BG646425 weakly similar to GP|11994649|db gene_id:MSD21.13~unkno... 27 9.7
TC80168 similar to PIR|T00487|T00487 probable potassium transpor... 27 9.7
TC87336 similar to GP|10178138|dbj|BAB11550. nucleic acid bindin... 27 9.7
>TC78009 similar to GP|19424097|gb|AAL87328.1 unknown protein {Arabidopsis
thaliana}, partial (35%)
Length = 1134
Score = 497 bits (1279), Expect = e-141
Identities = 254/281 (90%), Positives = 264/281 (93%)
Frame = +1
Query: 6 ILLLKSTYLHVYVSFESFSICYLLLQDAAKMYGAVLACTDEAVLPVSQVFDAIRELGNEG 65
I +L+++ ++ S + +L+ DAAKMYGAVLACTDEAVLPVSQVFDAIRELGNEG
Sbjct: 67 ISILEASIRFLFTLSLSLQLQHLIAMDAAKMYGAVLACTDEAVLPVSQVFDAIRELGNEG 246
Query: 66 VEKLDPLVITSASSLSKFPVESSSVDLVVLIWKSLDFPIDQLTQEVLRVLKAGGTTLIHK 125
VEKLDPLVITSASSLSKFPVESSSVDLVVLIWKSLDFPIDQLTQEVLRVLKAGGTTLI K
Sbjct: 247 VEKLDPLVITSASSLSKFPVESSSVDLVVLIWKSLDFPIDQLTQEVLRVLKAGGTTLIRK 426
Query: 126 SSQSAVGSGDKMIPDLENKLLLAGFSEIQALQSSVIKAKKPSWKIGSSFALKKFVKSSPK 185
SSQSAVGSGDKMIPDLENKLLLAGFSEIQALQSSVIKAKKPSWKIGSSFALKK VKSSPK
Sbjct: 427 SSQSAVGSGDKMIPDLENKLLLAGFSEIQALQSSVIKAKKPSWKIGSSFALKKVVKSSPK 606
Query: 186 VQIDFDSDLIDKNSLLSEEDLKKPELPSGDCEIGPTRKACKNCSCGRAEEEEKVLKLGLT 245
VQIDFDSDLID+NSLLSEEDLKKPELPSGDCEIGPTRKACKNCSCGRAEEEEKVLKLGLT
Sbjct: 607 VQIDFDSDLIDENSLLSEEDLKKPELPSGDCEIGPTRKACKNCSCGRAEEEEKVLKLGLT 786
Query: 246 AEQINNPQSACGSCGLGDAFRCSTCPYKGLPAFKMGETVLL 286
AEQINNPQSACGSCGLGDAFRCSTCPYKGLPAFKMGE V L
Sbjct: 787 AEQINNPQSACGSCGLGDAFRCSTCPYKGLPAFKMGEKVAL 909
>BF632953 similar to GP|19424097|gb| unknown protein {Arabidopsis thaliana},
partial (14%)
Length = 576
Score = 104 bits (260), Expect = 4e-23
Identities = 48/48 (100%), Positives = 48/48 (100%)
Frame = +1
Query: 211 LPSGDCEIGPTRKACKNCSCGRAEEEEKVLKLGLTAEQINNPQSACGS 258
LPSGDCEIGPTRKACKNCSCGRAEEEEKVLKLGLTAEQINNPQSACGS
Sbjct: 1 LPSGDCEIGPTRKACKNCSCGRAEEEEKVLKLGLTAEQINNPQSACGS 144
>TC83390 similar to SP|O75533|S3B1_HUMAN Splicing factor 3B subunit 1
(Spliceosome associated protein 155) (SAP 155)
(SF3b155), partial (5%)
Length = 892
Score = 38.1 bits (87), Expect = 0.004
Identities = 21/29 (72%), Positives = 22/29 (75%), Gaps = 1/29 (3%)
Frame = +1
Query: 259 CGLGDAFR-CSTCPYKGLPAFKMGETVLL 286
CGLGD FR CST PYKG PAFKM + V L
Sbjct: 646 CGLGDEFRCCSTHPYKG-PAFKMEKKVEL 729
>TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (69%)
Length = 1615
Score = 29.6 bits (65), Expect = 1.5
Identities = 14/36 (38%), Positives = 22/36 (60%)
Frame = +2
Query: 191 DSDLIDKNSLLSEEDLKKPELPSGDCEIGPTRKACK 226
D++ D++S S+E+ KKP + PT+KACK
Sbjct: 56 DTESSDEDSESSDEEDKKPAAKASKNVSAPTKKACK 163
>TC79150 similar to GP|18461304|dbj|BAB84499. putative receptor-like protein
kinase {Oryza sativa (japonica cultivar-group)}, partial
(7%)
Length = 1077
Score = 28.5 bits (62), Expect = 3.3
Identities = 27/88 (30%), Positives = 40/88 (44%), Gaps = 14/88 (15%)
Frame = +3
Query: 74 ITSASSLSKFPVE-----------SSSVDLVVLIWKSLDFPIDQLT--QEVLRVLKAGGT 120
I S SS S FP++ S++ + ++W+S DFP D L QE+
Sbjct: 384 IWSTSSFSPFPLQLKLQNNGNLVLSTTNGNISILWQSFDFPTDTLLPGQEINE-----RA 548
Query: 121 TLIHKSSQSAVGSG-DKMIPDLENKLLL 147
TL+ S++ SG K D +N L L
Sbjct: 549 TLVSSKSETNYSSGFYKFYFDNDNALRL 632
>TC86477 homologue to GP|15408819|dbj|BAB64211. tubulin beta-4 chain {Oryza
sativa (japonica cultivar-group)}, partial (98%)
Length = 1816
Score = 28.1 bits (61), Expect = 4.4
Identities = 15/47 (31%), Positives = 22/47 (45%)
Frame = +2
Query: 224 ACKNCSCGRAEEEEKVLKLGLTAEQINNPQSACGSCGLGDAFRCSTC 270
ACK+ C EEE+VL+ L + Q + + C L +F C
Sbjct: 467 ACKDFKCATPWEEERVLEWELYSFQRSEKNTLIE*CSLSLSFHLQRC 607
>BF634872
Length = 688
Score = 28.1 bits (61), Expect = 4.4
Identities = 28/86 (32%), Positives = 40/86 (45%), Gaps = 3/86 (3%)
Frame = +1
Query: 52 SQVFDAIRELGNEGVEKLDPLVITSASSLS---KFPVESSSVDLVVLIWKSLDFPIDQLT 108
S V DA+ + EG+ LD L+ T ++S + S VD VL S++ ID L
Sbjct: 262 SAVEDAVSKKIREGIPTLDNLLQTLPKTISIDETAALNISFVDNPVLSNSSIELEIDGLF 441
Query: 109 QEVLRVLKAGGTTLIHKSSQSAVGSG 134
E VL + H+ S +V SG
Sbjct: 442 TERNDVLV---PQVYHRRSDISVSSG 510
>BF637342 similar to SP|Q99334|MT1_CO Metallothionein-like protein CAP3. [)]
{cingulata}, complete
Length = 316
Score = 27.7 bits (60), Expect = 5.7
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +3
Query: 254 SACGSCGLGDAFRCSTCPYK 273
++ GSCG G + C+ CP+K
Sbjct: 105 ASSGSCGCGSSCTCAGCPHK 164
>TC86058 similar to GP|15912261|gb|AAL08264.1 AT4g27720/T29A15_210
{Arabidopsis thaliana}, partial (96%)
Length = 1979
Score = 27.7 bits (60), Expect = 5.7
Identities = 16/48 (33%), Positives = 24/48 (49%)
Frame = -3
Query: 142 ENKLLLAGFSEIQALQSSVIKAKKPSWKIGSSFALKKFVKSSPKVQID 189
E + + SE+ ++ S+ AK W I SSF L V + KV I+
Sbjct: 1086 EERAINLDASELPNIEDSMNVAKIKPWGISSSFGLNAGVHRNTKVYIE 943
>TC90776 similar to GP|7269000|emb|CAB80733.1 contains EST gb:AI998867.1
{Arabidopsis thaliana}, partial (17%)
Length = 1264
Score = 27.7 bits (60), Expect = 5.7
Identities = 19/61 (31%), Positives = 32/61 (52%)
Frame = +2
Query: 152 EIQALQSSVIKAKKPSWKIGSSFALKKFVKSSPKVQIDFDSDLIDKNSLLSEEDLKKPEL 211
E Q + + A KPS+++ S L + + V+ DF+ DK +L+EE+ +PE
Sbjct: 758 EGQMVDGILTSASKPSYELPSQEELIRQAFAGDDVEDDFEK---DKQEILNEEN-PEPEK 925
Query: 212 P 212
P
Sbjct: 926 P 928
>BQ144005
Length = 769
Score = 27.3 bits (59), Expect = 7.5
Identities = 20/66 (30%), Positives = 37/66 (55%), Gaps = 4/66 (6%)
Frame = -1
Query: 2 QSFFILLLKSTYLHVYVSFESF-SICYLLL---QDAAKMYGAVLACTDEAVLPVSQVFDA 57
+SF I K +++S +S+ + CYL+L +D + V+ CT A + +S+ + +
Sbjct: 571 KSFLIRDGKRRRF*IFLSTKSWQNQCYLVLTL*EDFLC*FLRVIVCT*HAPVKLSREYSS 392
Query: 58 IRELGN 63
+R LGN
Sbjct: 391 LRHLGN 374
>TC88968 similar to GP|12329988|emb|CAC24566. trypsin inhibitor {Pisum
sativum}, partial (73%)
Length = 546
Score = 26.9 bits (58), Expect = 9.7
Identities = 13/25 (52%), Positives = 15/25 (60%), Gaps = 2/25 (8%)
Frame = +1
Query: 209 PELPSGDC-EIGPT-RKACKNCSCG 231
PEL C ++G T ACKNC CG
Sbjct: 274 PELTQCRCADVGKTCHSACKNCECG 348
>BG646425 weakly similar to GP|11994649|db gene_id:MSD21.13~unknown protein
{Arabidopsis thaliana}, partial (33%)
Length = 802
Score = 26.9 bits (58), Expect = 9.7
Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = +2
Query: 213 SGDCEIGPTRKACKNC-SCGRAEEEEKVLK 241
S DC++G + K+ KNC ++EEE K
Sbjct: 383 SHDCDVGSSSKSSKNCHEMDSSDEEESTWK 472
>TC80168 similar to PIR|T00487|T00487 probable potassium transport protein
F19I3.29 - Arabidopsis thaliana, partial (48%)
Length = 1175
Score = 26.9 bits (58), Expect = 9.7
Identities = 11/28 (39%), Positives = 13/28 (46%)
Frame = -1
Query: 251 NPQSACGSCGLGDAFRCSTCPYKGLPAF 278
N CG CGL + C C YK P +
Sbjct: 521 ND*KGCGDCGLACNYCC*RCNYKYRPVY 438
>TC87336 similar to GP|10178138|dbj|BAB11550. nucleic acid binding
protein-like {Arabidopsis thaliana}, partial (95%)
Length = 1223
Score = 26.9 bits (58), Expect = 9.7
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +3
Query: 208 KPELPSGDCEIGPTRKACKNCSCGRAEEEEKVLK-LGLTAEQINNPQSACGSCG 260
KP + SG G T+++ S G+ + K++ G E+ + ++ CGSCG
Sbjct: 630 KPTVDSGSKSRGSTKRS----SDGQVKSNPKLVDDQGYEEEEDEHSETLCGSCG 779
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.135 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,039,204
Number of Sequences: 36976
Number of extensions: 143243
Number of successful extensions: 744
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 742
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 744
length of query: 304
length of database: 9,014,727
effective HSP length: 96
effective length of query: 208
effective length of database: 5,465,031
effective search space: 1136726448
effective search space used: 1136726448
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146776.17