BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146758.2 + phase: 0
(74 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77354 similar to GP|20975622|emb|CAD31716. putative ripening r... 133 1e-32
AW257530 similar to GP|20975622|emb putative ripening related pr... 131 4e-32
TC85239 similar to GP|20975622|emb|CAD31716. putative ripening r... 107 7e-25
TC87805 similar to GP|20975622|emb|CAD31716. putative ripening r... 107 7e-25
TC85233 similar to GP|20975622|emb|CAD31716. putative ripening r... 105 3e-24
TC85246 similar to GP|20975622|emb|CAD31716. putative ripening r... 103 9e-24
TC85248 similar to GP|20975622|emb|CAD31716. putative ripening r... 103 1e-23
TC85241 similar to GP|20975622|emb|CAD31716. putative ripening r... 101 5e-23
TC85255 similar to GP|20975622|emb|CAD31716. putative ripening r... 100 8e-23
AL389514 similar to GP|20975622|emb putative ripening related pr... 98 7e-22
BF633501 58 8e-10
TC85261 weakly similar to GP|7406710|emb|CAB85634.1 putative rip... 57 2e-09
TC86209 weakly similar to SP|Q9SSK5|ML43_ARATH MLP-like protein ... 40 1e-04
BG451415 weakly similar to SP|Q9SSK5|ML43_ MLP-like protein 43. ... 38 6e-04
TC85257 similar to GP|20975622|emb|CAD31716. putative ripening r... 37 0.001
AW203185 33 0.016
AL389009 similar to GP|20975622|emb putative ripening related pr... 32 0.035
TC86345 homologue to SP|Q94DM8|U185_ORYSA Hypothetical protein P... 28 0.86
TC80672 weakly similar to GP|17380686|gb|AAL36173.1 unknown prot... 26 3.3
BF649819 similar to GP|12321880|gb| hypothetical protein; 64772-... 26 3.3
>TC77354 similar to GP|20975622|emb|CAD31716. putative ripening related
protein {Cicer arietinum}, complete
Length = 759
Score = 133 bits (335), Expect = 1e-32
Identities = 62/62 (100%), Positives = 62/62 (100%)
Frame = +1
Query: 1 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 60
MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH
Sbjct: 82 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 261
Query: 61 VR 62
VR
Sbjct: 262 VR 267
Score = 26.2 bits (56), Expect = 2.5
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +2
Query: 59 THVRVLSSKQRLLHKS 74
T + +LSSKQRLLHKS
Sbjct: 512 TLMLILSSKQRLLHKS 559
>AW257530 similar to GP|20975622|emb putative ripening related protein
{Cicer arietinum}, partial (37%)
Length = 722
Score = 131 bits (330), Expect = 4e-32
Identities = 61/61 (100%), Positives = 61/61 (100%)
Frame = +3
Query: 2 VLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTHV 61
VLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTHV
Sbjct: 3 VLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTHV 182
Query: 62 R 62
R
Sbjct: 183R 185
>TC85239 similar to GP|20975622|emb|CAD31716. putative ripening related
protein {Cicer arietinum}, partial (98%)
Length = 838
Score = 107 bits (268), Expect = 7e-25
Identities = 45/61 (73%), Positives = 55/61 (89%)
Frame = +3
Query: 1 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 60
MVLAGKL TE+GIK ++KFFKLFAS +HEVQN+CE VH TKLH+G++WHH+DSVKHWT+
Sbjct: 72 MVLAGKLITELGIKTPAEKFFKLFASELHEVQNVCERVHHTKLHEGEDWHHTDSVKHWTY 251
Query: 61 V 61
V
Sbjct: 252 V 254
>TC87805 similar to GP|20975622|emb|CAD31716. putative ripening related
protein {Cicer arietinum}, complete
Length = 694
Score = 107 bits (268), Expect = 7e-25
Identities = 47/61 (77%), Positives = 55/61 (90%)
Frame = +2
Query: 1 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 60
MVLAGK+STE+G+KASS KFFKLF+S +H VQN+CE VHETKLHQGD+WH SDSVK WT+
Sbjct: 14 MVLAGKISTEVGVKASSAKFFKLFSSELHHVQNLCERVHETKLHQGDDWHGSDSVKQWTY 193
Query: 61 V 61
V
Sbjct: 194V 196
>TC85233 similar to GP|20975622|emb|CAD31716. putative ripening related
protein {Cicer arietinum}, complete
Length = 840
Score = 105 bits (262), Expect = 3e-24
Identities = 45/74 (60%), Positives = 59/74 (78%)
Frame = +1
Query: 1 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 60
MVL+GKLSTE+GIK +DKF+ LF + +HEVQN+CE VH TKLH+G++WHH+DSVKHWT+
Sbjct: 136 MVLSGKLSTEVGIKTPADKFYNLFITELHEVQNLCERVHHTKLHEGEDWHHTDSVKHWTY 315
Query: 61 VRVLSSKQRLLHKS 74
V+ K H+S
Sbjct: 316 --VIDGKVHTCHES 351
>TC85246 similar to GP|20975622|emb|CAD31716. putative ripening related
protein {Cicer arietinum}, complete
Length = 724
Score = 103 bits (258), Expect = 9e-24
Identities = 44/61 (72%), Positives = 53/61 (86%)
Frame = +1
Query: 1 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 60
MVLAGKL TE+GIK +D+FFKLFAS +HEVQ CE VH TKLH+G++WHH+DSVKHWT+
Sbjct: 67 MVLAGKLMTELGIKTPADRFFKLFASELHEVQKHCERVHHTKLHEGEDWHHTDSVKHWTY 246
Query: 61 V 61
V
Sbjct: 247 V 249
>TC85248 similar to GP|20975622|emb|CAD31716. putative ripening related
protein {Cicer arietinum}, complete
Length = 919
Score = 103 bits (257), Expect = 1e-23
Identities = 47/74 (63%), Positives = 58/74 (77%)
Frame = +1
Query: 1 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 60
MVL GKLSTE+GIKAS DKF+ FA+ +HE+Q CE VHETKLHQGD+WHH+D+VKHWT+
Sbjct: 31 MVLNGKLSTELGIKASVDKFYNFFATQLHEMQIHCERVHETKLHQGDDWHHTDTVKHWTY 210
Query: 61 VRVLSSKQRLLHKS 74
V+ K H+S
Sbjct: 211 --VIDGKVHTCHES 246
>TC85241 similar to GP|20975622|emb|CAD31716. putative ripening related
protein {Cicer arietinum}, partial (83%)
Length = 867
Score = 101 bits (252), Expect = 5e-23
Identities = 42/61 (68%), Positives = 52/61 (84%)
Frame = +1
Query: 1 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 60
MVLAGKL TE+GIK+ +KFFKLFAS +HEV +CE VH KLH+G++WHH+DSVKHWT+
Sbjct: 67 MVLAGKLITELGIKSPGEKFFKLFASELHEVHKVCERVHHAKLHEGEDWHHNDSVKHWTY 246
Query: 61 V 61
V
Sbjct: 247 V 249
>TC85255 similar to GP|20975622|emb|CAD31716. putative ripening related
protein {Cicer arietinum}, partial (98%)
Length = 886
Score = 100 bits (250), Expect = 8e-23
Identities = 42/61 (68%), Positives = 51/61 (82%)
Frame = +2
Query: 1 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 60
MVL GKL TE+GIKA +KFFKLFAS +HEVQ +CE +H KLH+G++WHH+DSVKHWT
Sbjct: 83 MVLVGKLITELGIKAPGEKFFKLFASELHEVQKVCERIHHAKLHEGEDWHHNDSVKHWTI 262
Query: 61 V 61
V
Sbjct: 263 V 265
>AL389514 similar to GP|20975622|emb putative ripening related protein {Cicer
arietinum}, partial (54%)
Length = 498
Score = 97.8 bits (242), Expect = 7e-22
Identities = 41/60 (68%), Positives = 49/60 (81%)
Frame = -2
Query: 2 VLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTHV 61
VL GKL TE+GIKA +KFFKLFAS +HEVQ +CE +H KLH+G++WHH DSVKHWT V
Sbjct: 251 VLVGKLITELGIKAPREKFFKLFASELHEVQKVCERIHHAKLHEGEDWHHHDSVKHWTIV 72
>BF633501
Length = 352
Score = 57.8 bits (138), Expect = 8e-10
Identities = 26/58 (44%), Positives = 37/58 (62%)
Frame = +3
Query: 3 LAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHWTH 60
L+GK+ TEI I+AS KF+ +F + + NI VH K+H+GD W + SVKHW +
Sbjct: 39 LSGKVETEIEIQASGAKFYNIFRKQLEHLPNIATEVHGGKVHEGD-WENIGSVKHWEY 209
>TC85261 weakly similar to GP|7406710|emb|CAB85634.1 putative
ripening-related protein {Vitis vinifera}, partial
(15%)
Length = 1002
Score = 56.6 bits (135), Expect = 2e-09
Identities = 22/58 (37%), Positives = 36/58 (61%)
Frame = +2
Query: 1 MVLAGKLSTEIGIKASSDKFFKLFASNIHEVQNICESVHETKLHQGDEWHHSDSVKHW 58
M L+GK+ E+ I+A KF+ +F + + NI +HE ++H+GD W + S+KHW
Sbjct: 11 MALSGKVEAEVEIQAPGAKFYNIFRKQLEHLPNISNEIHEARVHEGD-WENIGSIKHW 181
>TC86209 weakly similar to SP|Q9SSK5|ML43_ARATH MLP-like protein 43.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (41%)
Length = 807
Score = 40.4 bits (93), Expect = 1e-04
Identities = 20/69 (28%), Positives = 38/69 (54%), Gaps = 4/69 (5%)
Frame = +1
Query: 6 KLSTEIGIKASSDKFFKLFASNIHEVQNIC-ESVHETKLHQGDEWHHSDSVKHWTHV--- 61
K+ ++ IKA+ +F+++ + H + N+C + V +LH+GD W + W +V
Sbjct: 82 KIEMDVPIKATPQQFYEVLCNKTHHISNVCPDIVKGIELHEGD-WGTEGCIISWNYVFAG 258
Query: 62 RVLSSKQRL 70
+ SKQR+
Sbjct: 259 KTCISKQRI 285
>BG451415 weakly similar to SP|Q9SSK5|ML43_ MLP-like protein 43. [Mouse-ear
cress] {Arabidopsis thaliana}, partial (20%)
Length = 546
Score = 38.1 bits (87), Expect = 6e-04
Identities = 16/57 (28%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
Frame = +1
Query: 6 KLSTEIGIKASSDKFFKLFASNIHEVQNIC-ESVHETKLHQGDEWHHSDSVKHWTHV 61
K+ ++ IKA+ +F+++ + H + N+C + V +LH+GD W + W +V
Sbjct: 52 KIEMDVPIKATPQQFYEVLCNKTHHISNVCPDIVKGIELHEGD-WGTEGCIISWNYV 219
>TC85257 similar to GP|20975622|emb|CAD31716. putative ripening related
protein {Cicer arietinum}, partial (68%)
Length = 523
Score = 37.0 bits (84), Expect = 0.001
Identities = 14/28 (50%), Positives = 20/28 (71%)
Frame = +3
Query: 47 DEWHHSDSVKHWTHVRVLSSKQRLLHKS 74
++WHH+DSVKHWT+ V+ K H+S
Sbjct: 12 EDWHHNDSVKHWTY--VIDGKVHTCHES 89
>AW203185
Length = 382
Score = 33.5 bits (75), Expect = 0.016
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +1
Query: 33 NICESVHETKLHQGDEWHHSDSVKHWTH 60
NI VH K+H+GD W + SVKHW +
Sbjct: 7 NIATEVHGGKVHEGD-WENIGSVKHWEY 87
>AL389009 similar to GP|20975622|emb putative ripening related protein {Cicer
arietinum}, partial (10%)
Length = 434
Score = 32.3 bits (72), Expect = 0.035
Identities = 21/50 (42%), Positives = 30/50 (60%), Gaps = 10/50 (20%)
Frame = +1
Query: 1 MVLAGKLSTEIGIKA-SSDKFFKLFA-----SNIH----EVQNICESVHE 40
MVLAGKL TE+GIK+ D+FFK +N+H + + C+ +HE
Sbjct: 82 MVLAGKLITELGIKSPDGDRFFKSNIAYRNFTNVHXSLSKRVHXCQRLHE 231
>TC86345 homologue to SP|Q94DM8|U185_ORYSA Hypothetical protein P0483G10.15.
[Rice] {Oryza sativa}, partial (85%)
Length = 628
Score = 27.7 bits (60), Expect = 0.86
Identities = 10/30 (33%), Positives = 14/30 (46%)
Frame = -2
Query: 20 FFKLFASNIHEVQNICESVHETKLHQGDEW 49
+F LF H + N+C +H H G W
Sbjct: 561 YFPLFPLQTHMLNNLCTILHYKHSHYGRRW 472
>TC80672 weakly similar to GP|17380686|gb|AAL36173.1 unknown protein
{Arabidopsis thaliana}, partial (68%)
Length = 1367
Score = 25.8 bits (55), Expect = 3.3
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = -2
Query: 24 FASNIHEVQNICESVHETKLHQGDEWHHSDSVKHW 58
+A I ++Q IC S HET HS S+ HW
Sbjct: 322 YALCIIKIQIICSSCHET--------CHSKSLAHW 242
>BF649819 similar to GP|12321880|gb| hypothetical protein; 64772-65429
{Arabidopsis thaliana}, partial (51%)
Length = 659
Score = 25.8 bits (55), Expect = 3.3
Identities = 12/51 (23%), Positives = 25/51 (48%), Gaps = 3/51 (5%)
Frame = +1
Query: 27 NIHEVQNICESVHETK---LHQGDEWHHSDSVKHWTHVRVLSSKQRLLHKS 74
N ++ +C ++ K LH +W + + HWT+ VL ++ + K+
Sbjct: 451 NQNQKSGVCMQMNAGKIQCLHTHVQWENKWNTHHWTYG*VLCNRDSIFFKN 603
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.129 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,574,968
Number of Sequences: 36976
Number of extensions: 33580
Number of successful extensions: 242
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 239
length of query: 74
length of database: 9,014,727
effective HSP length: 50
effective length of query: 24
effective length of database: 7,165,927
effective search space: 171982248
effective search space used: 171982248
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)
Medicago: description of AC146758.2