BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146757.7 - phase: 0
(367 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83019 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogen... 320 e-167
TC93120 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogen... 278 8e-90
TC86951 homologue to GP|2745902|gb|AAB94773.1| ERS-like ethylene... 31 0.67
TC80227 similar to GP|13537198|dbj|BAB40775. histidine kinase {A... 30 1.1
BI266143 similar to GP|13537196|d histidine kinase {Arabidopsis ... 30 1.5
BQ144031 similar to GP|3132295|dbj| long ORF {TT virus}, partial... 30 1.5
AW686764 similar to GP|13537198|d histidine kinase {Arabidopsis ... 30 1.9
TC89818 similar to GP|1448917|gb|AAB04607.1| threonine synthase ... 29 2.5
TC82875 similar to GP|15131529|emb|CAC48384. ethylene receptor {... 29 2.5
TC80045 similar to GP|21539495|gb|AAM53300.1 putative phosphatid... 29 2.5
AW585318 similar to PIR|T04590|T045 hypothetical protein F23E13.... 28 5.6
TC83618 28 7.4
TC80132 similar to PIR|D96538|D96538 cytosolic tRNA-Ala syntheta... 28 7.4
TC79346 similar to GP|9294216|dbj|BAB02118.1 contains similarity... 27 9.6
BI266474 27 9.6
>TC83019 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogenase kinase
{Arabidopsis thaliana}, partial (89%)
Length = 1140
Score = 320 bits (819), Expect(2) = e-167
Identities = 155/193 (80%), Positives = 176/193 (90%)
Frame = +3
Query: 22 KQTGVSLRYMMEFGSQPTDKNLLISAQFLQKELAIRIARRAIELESLPYGLSKKPAILKV 81
KQTGVSLRYMM+FGS+PT KNLLISAQFL KELAIRIARRA+ELE LP LS+KPA+LKV
Sbjct: 6 KQTGVSLRYMMDFGSKPTSKNLLISAQFLHKELAIRIARRAVELERLPCDLSQKPAVLKV 185
Query: 82 RDWYVDSFRDIRSCPEVKDMKDEREFTDVIKAIKVRHNNVVPTMALGVQQLKKELKTKID 141
RDWY+DSFRDIRS E+K+M DE+EFT+VIKAIKVRHNNVVPTMALGVQQLKK++ KI
Sbjct: 186 RDWYLDSFRDIRSFSEIKNMNDEKEFTEVIKAIKVRHNNVVPTMALGVQQLKKDVDPKII 365
Query: 142 SEDLVEIHEFLDRFYLSRIGVRMLIGQHVELHNPNPPPHVVGYIHTKMSPVSVARNASED 201
++D VEIH+FLDRFY+SRIG+RMLIGQHVELH+PNPPPHVV YIHT+MSPV VARNA +D
Sbjct: 366 NQDFVEIHQFLDRFYMSRIGIRMLIGQHVELHSPNPPPHVVSYIHTRMSPVEVARNAVDD 545
Query: 202 ARSICMREYGSAP 214
A SIC R+YGSAP
Sbjct: 546 APSICSRQYGSAP 584
Score = 287 bits (735), Expect(2) = e-167
Identities = 141/154 (91%), Positives = 148/154 (95%)
Frame = +1
Query: 214 PEINIYGDPDFTFPYVPAHLHLMVFELVKNSLRAVQERYMDSDKVSPPIRIIVADGLEDV 273
P+++IYGDP+FTFPYVPAHLHLMVFELVKNSLRAV+ERYM+SDK PPIRIIVADGLEDV
Sbjct: 583 PDVHIYGDPEFTFPYVPAHLHLMVFELVKNSLRAVEERYMNSDKDPPPIRIIVADGLEDV 762
Query: 274 TIKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVTTMAGYGYGLPISRLYA 333
TIKISDEGGGIPRSGL KIFTYLYSTARNPLDEHADLGV D+V TMAGYGYGLPISRLYA
Sbjct: 763 TIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNV-TMAGYGYGLPISRLYA 939
Query: 334 RYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP 367
RYFGGDLQIISME YGTDAYLHLSRLGDSQEPLP
Sbjct: 940 RYFGGDLQIISMENYGTDAYLHLSRLGDSQEPLP 1041
>TC93120 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogenase kinase
{Arabidopsis thaliana}, partial (45%)
Length = 635
Score = 278 bits (712), Expect(2) = 8e-90
Identities = 143/157 (91%), Positives = 148/157 (94%), Gaps = 1/157 (0%)
Frame = +3
Query: 1 MAAIEAFSKSLLQEVHKWGCLKQTGVSLRYMMEFGSQPTDKNLLISAQFLQKELAIRIAR 60
MAAIEAFSKSLLQEVHKWGCLKQTGVSLRYMMEFGSQPTDKNLLISAQFLQKELAIRIAR
Sbjct: 72 MAAIEAFSKSLLQEVHKWGCLKQTGVSLRYMMEFGSQPTDKNLLISAQFLQKELAIRIAR 251
Query: 61 RAIELESLPYGLSKKPAILKVRDWYVDSFRDIRSCPEVKDMKDEREFTDVIKAIKVRHNN 120
RAIELESLPYGLSKKPAILKVRDWYVDSFRDIRSCPEVKDMKDEREFTDVIKAIKVRHNN
Sbjct: 252 RAIELESLPYGLSKKPAILKVRDWYVDSFRDIRSCPEVKDMKDEREFTDVIKAIKVRHNN 431
Query: 121 VVPTMALGVQQLKKELKTK-IDSEDLVEIHEFLDRFY 156
VVPTMALGVQQLKKE + K + + L+ +FLDRFY
Sbjct: 432 VVPTMALGVQQLKKEFENKRLIVKILLRFMKFLDRFY 542
Score = 70.1 bits (170), Expect(2) = 8e-90
Identities = 31/32 (96%), Positives = 31/32 (96%)
Frame = +1
Query: 155 FYLSRIGVRMLIGQHVELHNPNPPPHVVGYIH 186
F LSRIGVRMLIGQHVELHNPNPPPHVVGYIH
Sbjct: 538 FTLSRIGVRMLIGQHVELHNPNPPPHVVGYIH 633
>TC86951 homologue to GP|2745902|gb|AAB94773.1| ERS-like ethylene receptor
{Pisum sativum}, complete
Length = 2274
Score = 31.2 bits (69), Expect = 0.67
Identities = 23/85 (27%), Positives = 35/85 (41%)
Frame = +3
Query: 267 ADGLEDVTIKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVTTMAGYGYGL 326
+DG + +++ D G GI +P +FT + P V + +G G GL
Sbjct: 1668 SDGHFYIRVQVKDSGCGILPQDIPHLFTKFTQSQSGP------------VRSSSGSGLGL 1811
Query: 327 PISRLYARYFGGDLQIISMEGYGTD 351
I + + GG I ME G D
Sbjct: 1812 AICKRFVNLMGGH---IWMESEGHD 1877
>TC80227 similar to GP|13537198|dbj|BAB40775. histidine kinase {Arabidopsis
thaliana}, partial (22%)
Length = 1693
Score = 30.4 bits (67), Expect = 1.1
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +3
Query: 270 LEDVTIKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVT 317
L ++ + + D G GIPR P IFT + +H G+ S++
Sbjct: 6 LINLIVSVEDTGDGIPRESQPLIFTPFMQVGSSISRKHGGTGIGLSIS 149
>BI266143 similar to GP|13537196|d histidine kinase {Arabidopsis thaliana},
partial (9%)
Length = 470
Score = 30.0 bits (66), Expect = 1.5
Identities = 23/78 (29%), Positives = 31/78 (39%), Gaps = 2/78 (2%)
Frame = +2
Query: 275 IKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVT--TMAGYGYGLPISRLY 332
+ + D G GIP +IFT ADS T T G G GL IS+
Sbjct: 86 VTVEDTGIGIPTDAQSRIFTPFMQ--------------ADSSTSRTYGGTGIGLSISKCL 223
Query: 333 ARYFGGDLQIISMEGYGT 350
GG++ +S G+
Sbjct: 224 VDLMGGEIGFVSEPEIGS 277
>BQ144031 similar to GP|3132295|dbj| long ORF {TT virus}, partial (20%)
Length = 1411
Score = 30.0 bits (66), Expect = 1.5
Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 9/63 (14%)
Frame = +3
Query: 155 FYLSRIGVRMLIGQHVELHNPNPPPHVVGYIHT---------KMSPVSVARNASEDARSI 205
F+++ + + + + E HN PH VG+ HT +P+S+ R AS AR
Sbjct: 72 FFITNLSIHNTLNKK-EKHNSPTTPHAVGFAHTINPFFGGPLVPAPISITR-ASPIARQS 245
Query: 206 CMR 208
C R
Sbjct: 246 CPR 254
>AW686764 similar to GP|13537198|d histidine kinase {Arabidopsis thaliana},
partial (12%)
Length = 652
Score = 29.6 bits (65), Expect = 1.9
Identities = 20/84 (23%), Positives = 32/84 (37%)
Frame = +2
Query: 267 ADGLEDVTIKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVTTMAGYGYGL 326
++ L + + + D G GIP P IFT + +H G G GL
Sbjct: 302 SNDLISLIVSVEDTGDGIPLESQPMIFTPFMQVGPSISRKH------------GGTGIGL 445
Query: 327 PISRLYARYFGGDLQIISMEGYGT 350
IS+ G++ +S G+
Sbjct: 446 SISKCLVGLMNGEIGFVSEPKIGS 517
>TC89818 similar to GP|1448917|gb|AAB04607.1| threonine synthase
{Arabidopsis thaliana}, partial (32%)
Length = 684
Score = 29.3 bits (64), Expect = 2.5
Identities = 9/30 (30%), Positives = 19/30 (63%)
Frame = +1
Query: 169 HVELHNPNPPPHVVGYIHTKMSPVSVARNA 198
H+ H+P+PPP +H ++SP + ++ +
Sbjct: 55 HITAHSPSPPPPPTTTLHHRISPPTTSKTS 144
>TC82875 similar to GP|15131529|emb|CAC48384. ethylene receptor {Fragaria x
ananassa}, partial (39%)
Length = 1118
Score = 29.3 bits (64), Expect = 2.5
Identities = 25/92 (27%), Positives = 40/92 (43%), Gaps = 1/92 (1%)
Frame = +3
Query: 275 IKISDEGGGIPRSGLPKIFTYLYSTARNPLDEHADLGVADSVTTMAGYGYGLPISRLYAR 334
+++ D G GI +PK+FT ++ LG + V G G GL I + +
Sbjct: 234 VQVKDSGSGINPQDIPKLFT--------KFAQNQTLGTKNPV----GSGLGLAICKRFVN 377
Query: 335 YFGGDLQIISMEGYGTDAYL-HLSRLGDSQEP 365
G + I S EG G + + +LG + P
Sbjct: 378 LMEGHIWIES-EGIGKGCTVTFIVKLGIADRP 470
>TC80045 similar to GP|21539495|gb|AAM53300.1 putative
phosphatidylinositol-4-phosphate 5-kinase {Arabidopsis
thaliana}, partial (45%)
Length = 1798
Score = 29.3 bits (64), Expect = 2.5
Identities = 17/67 (25%), Positives = 33/67 (48%), Gaps = 2/67 (2%)
Frame = +1
Query: 104 EREFTDVIKAIKVRHNNVVPTMALGVQQLKKELKTKI--DSEDLVEIHEFLDRFYLSRIG 161
ERE+ + +V NN ++ +QLKK++ I E +++ H D ++G
Sbjct: 1450 EREYMQGVLISEVVLNNSFASLTRRAKQLKKKIAKDIKRPGEQIIKGHRSYDLMLSLQLG 1629
Query: 162 VRMLIGQ 168
+R +G+
Sbjct: 1630 IRYTVGK 1650
>AW585318 similar to PIR|T04590|T045 hypothetical protein F23E13.100 -
Arabidopsis thaliana, partial (19%)
Length = 585
Score = 28.1 bits (61), Expect = 5.6
Identities = 25/110 (22%), Positives = 48/110 (42%)
Frame = +2
Query: 39 TDKNLLISAQFLQKELAIRIARRAIELESLPYGLSKKPAILKVRDWYVDSFRDIRSCPEV 98
+D N SAQFL+ ELA+ A A+ LE ++ + Y R+ S P+V
Sbjct: 5 SDDNAA*SAQFLELELALSKAGDAVVLEMEKNAHPSNSKRERINE-YEHQCREKYSAPDV 181
Query: 99 KDMKDEREFTDVIKAIKVRHNNVVPTMALGVQQLKKELKTKIDSEDLVEI 148
+ ++ + + + + P + L ++ +KID + + E+
Sbjct: 182 QSNPEKADVD--VNLDNQKETDAAPLINLEDPPQQRSDNSKIDEKPIEEV 325
>TC83618
Length = 807
Score = 27.7 bits (60), Expect = 7.4
Identities = 14/31 (45%), Positives = 18/31 (57%), Gaps = 5/31 (16%)
Frame = +2
Query: 219 YGDPDFTFP-----YVPAHLHLMVFELVKNS 244
Y +P F FP YVPAHL L +F + +S
Sbjct: 449 YSNPHFVFPHSHWLYVPAHLKLNLFAALISS 541
>TC80132 similar to PIR|D96538|D96538 cytosolic tRNA-Ala synthetase
[imported] - Arabidopsis thaliana, partial (25%)
Length = 908
Score = 27.7 bits (60), Expect = 7.4
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1
Query: 324 YGLPISRLYARYFGGD 339
Y LP R+Y YFGGD
Sbjct: 529 YNLPSDRIYVTYFGGD 576
>TC79346 similar to GP|9294216|dbj|BAB02118.1 contains similarity to unknown
protein~gb|AAB61017.1~gene_id:K17E12.5 {Arabidopsis
thaliana}, partial (96%)
Length = 1707
Score = 27.3 bits (59), Expect = 9.6
Identities = 24/99 (24%), Positives = 44/99 (44%), Gaps = 8/99 (8%)
Frame = +1
Query: 118 HNNVVPTMALGVQQLKKELKTKIDSEDLVEIHEFLDRFYLS-------RIGVRMLIGQHV 170
H N+V + + + ++ K +++ +D E H L S I + ++ H+
Sbjct: 55 HPNLVLHLQISQRWVQFH*KLEMEQQDSKEQHFVLQLLTFS*FSVSSQPISLLSMLSLHL 234
Query: 171 -ELHNPNPPPHVVGYIHTKMSPVSVARNASEDARSICMR 208
+ P P P V I TK S +S ++ S R IC++
Sbjct: 235 PQKTTPPPTPTTVTLIFTKTSLLSQNKSHSYSER*ICLK 351
>BI266474
Length = 366
Score = 27.3 bits (59), Expect = 9.6
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +3
Query: 157 LSRIGVRMLIGQHVELHNPNPPPHVVGYIHTKMSPVSVARN 197
+SR V +LI NPN V YIHT ++ +S+ N
Sbjct: 6 VSRGEVELLILNSAVYTNPNNSSRVSSYIHT*LTQLSILTN 128
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.139 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,794,594
Number of Sequences: 36976
Number of extensions: 123882
Number of successful extensions: 670
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 667
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 669
length of query: 367
length of database: 9,014,727
effective HSP length: 97
effective length of query: 270
effective length of database: 5,428,055
effective search space: 1465574850
effective search space used: 1465574850
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146757.7