BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146757.5 - phase: 0 /pseudo
(588 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI310247 weakly similar to PIR|T05213|T05 hypothetical protein F... 357 e-119
TC81274 similar to GP|23429644|gb|AAN10199.1 endoxylanase {Caric... 417 e-117
TC87528 similar to PIR|D96617|D96617 probable xylan endohydrolas... 236 1e-62
AW774387 weakly similar to PIR|T05210|T05 hypothetical protein F... 171 6e-43
BE317888 similar to PIR|T05693|T056 hypothetical protein F20M13.... 64 2e-10
AW688565 similar to PIR|D96617|D96 probable xylan endohydrolase ... 36 0.047
AW689011 similar to PIR|D96617|D96 probable xylan endohydrolase ... 34 0.18
TC86597 weakly similar to GP|9837280|gb|AAG00510.1| leaf senesce... 31 1.5
TC78251 weakly similar to GP|9758571|dbj|BAB09184.1 N-hydroxycin... 30 3.4
BF648593 similar to GP|22770464|gb anthranilate N-hydroxycinamoy... 29 4.4
BF521097 similar to GP|22770464|gb| anthranilate N-hydroxycinamo... 29 4.4
TC79081 weakly similar to GP|9758571|dbj|BAB09184.1 N-hydroxycin... 29 4.4
TC77864 weakly similar to PIR|T06329|T06329 symbiotic ammonium t... 29 5.8
TC90412 homologue to SP|P23704|ATPB_NEUCR ATP synthase beta chai... 29 5.8
AW587067 similar to PIR|T09584|T09 high mobility group protein H... 28 7.6
TC90305 similar to PIR|D96610|D96610 probable integral membrane ... 28 7.6
TC79782 weakly similar to GP|11994288|dbj|BAB01471. alanine acet... 28 7.6
>BI310247 weakly similar to PIR|T05213|T05 hypothetical protein F17I5.40 -
Arabidopsis thaliana, partial (39%)
Length = 841
Score = 357 bits (916), Expect(2) = e-119
Identities = 169/212 (79%), Positives = 192/212 (89%)
Frame = +1
Query: 30 ITTGFEAEALSYDYSANVECLAHPQKPQYNGGIIKNPELNDGLQGWTTFGDAIIEHRKSL 89
+ GFEAEALSYD+SA++ECLAHPQKPQYNGGIIKNPELNDGLQGWT FG+A IEHR+SL
Sbjct: 34 LLAGFEAEALSYDHSASIECLAHPQKPQYNGGIIKNPELNDGLQGWTAFGNARIEHRESL 213
Query: 90 GNKFVVTHSRNQPHDSVSQKIYLRKGLHYSLSAWIQVSEETVPVTAVVKTTKGFKFGGAI 149
GNK+VV ++RNQPHDSV+QKIYL+ GLHYSLSAWIQVSE V VTA+VKTT+GFKFGGAI
Sbjct: 214 GNKYVVANNRNQPHDSVAQKIYLQNGLHYSLSAWIQVSEANVLVTAMVKTTEGFKFGGAI 393
Query: 150 FAEPNCWSMLKGGLIADTTGVAELYFESNNTSVEIWVDNVSLQPFTEKQWRSHQELSIEK 209
+AEPNCWSMLKGGL AD T VAELYFESNNTSVEIW+DN+SLQPFT+KQW SHQE +IEK
Sbjct: 394 YAEPNCWSMLKGGLTADATEVAELYFESNNTSVEIWIDNISLQPFTKKQWTSHQEQNIEK 573
Query: 210 DRKRKVVVRAVNEQGHPLPNASISLTMKRPGF 241
RK+ VVV+A++EQG PLPNA IS+TMKR F
Sbjct: 574 TRKKNVVVQALDEQGRPLPNARISITMKRYWF 669
Score = 90.9 bits (224), Expect(2) = e-119
Identities = 41/57 (71%), Positives = 46/57 (79%)
Frame = +3
Query: 241 FPFGSAINKNILNNNAYQDWFASRFTVTTFENEMKWYTNEYAQGKDNYFDADAMLGF 297
F FGSA+NK IL+N AY WFASRFT T F NEMKWYTNEY QG+DNYF+AD ML +
Sbjct: 669 FHFGSAMNKYILSNTAYHYWFASRFTGTAF*NEMKWYTNEYTQGRDNYFEADVMLRY 839
>TC81274 similar to GP|23429644|gb|AAN10199.1 endoxylanase {Carica papaya},
partial (33%)
Length = 912
Score = 417 bits (1072), Expect = e-117
Identities = 196/241 (81%), Positives = 217/241 (89%)
Frame = +2
Query: 348 QLIGWDVVNENLHFSFFESKLGQNFSARMFNEVHNIDGQTTLFMNEYNTIEDSRDGLSTP 407
QLIGWDV+NENLH SFFESKLGQNF++RMFNEVHN+DG TTLFMN+YNTIEDSRDGLSTP
Sbjct: 2 QLIGWDVINENLHSSFFESKLGQNFTSRMFNEVHNVDGNTTLFMNDYNTIEDSRDGLSTP 181
Query: 408 PTYIEKIKEIQSVNSQLPLGIGLESHFPNSPPNLPYMRASLDTLRATGLPIWITELDVAS 467
P YI+KI+EIQS N QLPLGIGLESHFPN PPNLPYMRASLD L ATGLPIWITELDVAS
Sbjct: 182 PKYIQKIREIQSWNKQLPLGIGLESHFPNFPPNLPYMRASLDILAATGLPIWITELDVAS 361
Query: 468 QPNQALYFEQVLREAHSHPGIQGIVMWTAWSPQGCYRICLTDNNFKNLPAGDVVDQLINE 527
QPNQA Y EQVLREAHSHPGI+GIVMWTAWSPQGC+R+CLTDNNFKNLP GDVVD+LI E
Sbjct: 362 QPNQAGYLEQVLREAHSHPGIRGIVMWTAWSPQGCFRMCLTDNNFKNLPTGDVVDKLIYE 541
Query: 528 WGRAEKSGTTDQNGYFEASLFHGDYEIEINHPIKKKSNFTHHIKVLSKDEFKKTKQFIQL 587
WGR SGTTD+NG+ +ASLFHGDY++EI+H KK FTH ++VLSKD+ KKT QFIQ+
Sbjct: 542 WGRKILSGTTDKNGFLKASLFHGDYKMEISHSAKKNYTFTHQLQVLSKDKSKKTTQFIQI 721
Query: 588 S 588
S
Sbjct: 722 S 724
>TC87528 similar to PIR|D96617|D96617 probable xylan endohydrolase isoenzyme
F9K23.10 [imported] - Arabidopsis thaliana, partial
(67%)
Length = 2080
Score = 236 bits (603), Expect = 1e-62
Identities = 164/545 (30%), Positives = 261/545 (47%), Gaps = 34/545 (6%)
Frame = +1
Query: 54 QKPQYNGGIIKNPELNDGLQGWTTFGDAIIE------------HRKSLG------NKFVV 95
Q + II+N L+D +GW T G+ + R+SLG ++++
Sbjct: 271 QNVAFGVNIIENSNLSDDTKGWFTLGNCPLTVKTGSPHILPPMARESLGPHGILSGRYIL 450
Query: 96 THSRNQ----PHDSVSQKIYLRKGLHYSLSAWIQVSEET---VPVTAVVKTTKGFKFGGA 148
+R Q P +++K+ L L Y +SAW+++ + V + + GG
Sbjct: 451 VTNRTQTWMGPAQVITEKLKLF--LTYQVSAWVRIGSSSNGPQNVNVALGADNQWINGGQ 624
Query: 149 IFAEPNCWSMLKGGL-IADTTGVAELYFESNNTSVEIWVDNVSLQPFTEKQWRSHQELSI 207
+ W + G I +Y + + V++ V + + P + ++
Sbjct: 625 TEVSDDRWHEIGGSFRIEKQPTKIMVYIQGPASGVDLMVAGLQIFPVDRHARFRYLKMQT 804
Query: 208 EKDRKRKVVVRAVNEQGHPLPNASISLTMKRPGFPFGSAINKNILNNNAYQDWFASRFTV 267
+K RKR VV++ N + + + FP G+ I+++ ++N + ++ F
Sbjct: 805 DKIRKRDVVLKFAGLDSSSYLNTMVQVRQTQNNFPIGTCISRSNIDNEDFVNFLVKHFNW 984
Query: 268 TTFENEMKWYTNEYAQGKDNYFDADAMLGFAEKQGIAVRGHNIFWDDPQYQPNWVSSLSP 327
F NE+KWY E QG NY DAD +L +K I RGH IFW+ WV SL+
Sbjct: 985 AVFANELKWYWTEPQQGNLNYKDADDLLTLCQKYKIQTRGHCIFWEVDGTVQQWVKSLNK 1164
Query: 328 DQLNDAVEKRVNSIVSRYKGQLIGWDVVNENLHFSFFESKLGQNFSARMFNEVHNIDGQT 387
+ L AV+ R+ S+++RYKG+ +DV NE LH SF++ +LG++ A MF + +D
Sbjct: 1165NDLMTAVQNRLTSLLTRYKGKFSHYDVNNEMLHGSFYQDRLGKDIRANMFKTANQLDLSA 1344
Query: 388 TLFMNEYNTIEDSRDGLSTPPTYIEKIKEIQSVNSQLPLGIGLESHFPNSPPNLPYMRAS 447
TLF+N+Y+ IED D S P YIE I ++Q + + GIG++ H + P P + +S
Sbjct: 1345TLFVNDYH-IEDGCDTRSCPNKYIEHILDLQEQGAPVG-GIGIQGHIDS--PVGPVVCSS 1512
Query: 448 LDTLRATGLPIWITELDVASQPN--QALYFEQVLREAHSHPGIQGIVMWTAWSPQGCYRI 505
LD L GLPIW TELDV+S + E +LREA +HP ++GI++W W
Sbjct: 1513LDKLGILGLPIWFTELDVSSMNEYVRGDDLEVMLREAMAHPAVEGIMLWGFWE-----LF 1677
Query: 506 CLTDNNFKNLPAGDVVD------QLINEWGRAEKSGTTDQNGYFEASLFHGDYEIEINHP 559
DN GD+ + L EW + G ++ G F F+G Y +EI P
Sbjct: 1678MSRDNAHLVNAEGDINEAGKRFLALKQEW-LSHSHGHVNEQGQFNFRGFYGTYNVEIVTP 1854
Query: 560 IKKKS 564
KK S
Sbjct: 1855SKKIS 1869
>AW774387 weakly similar to PIR|T05210|T05 hypothetical protein F17I5.10 -
Arabidopsis thaliana (fragment), partial (19%)
Length = 658
Score = 171 bits (434), Expect = 6e-43
Identities = 82/177 (46%), Positives = 110/177 (61%), Gaps = 2/177 (1%)
Frame = -2
Query: 390 FMNEYNTIEDSRDGLSTPPTYIEKIKEIQSVN--SQLPLGIGLESHFPNSPPNLPYMRAS 447
FMNEYNTIE S D + +PP Y++K++EI +++ IGL+ HF + PNL YMR+
Sbjct: 657 FMNEYNTIEYSGDKVVSPPNYLKKLEEIMQSGEATEILFAIGLQGHFASGQPNLAYMRSG 478
Query: 448 LDTLRATGLPIWITELDVASQPNQALYFEQVLREAHSHPGIQGIVMWTAWSPQGCYRICL 507
LD L G PIW+TE + QPNQA YFE+VLREA+SHP ++GI+M+ + G L
Sbjct: 477 LDFLGNIGFPIWLTEASLDPQPNQAEYFEEVLREAYSHPAVEGIIMFAGPAQAGFNSTLL 298
Query: 508 TDNNFKNLPAGDVVDQLINEWGRAEKSGTTDQNGYFEASLFHGDYEIEINHPIKKKS 564
D NF+ P G VVD LI EWG + D G + SL HGDY++ HP+ + S
Sbjct: 297 ADTNFQTTPTGQVVDNLILEWGSGPYTAIADSRGIVDISLHHGDYDVTFTHPLTQNS 127
>BE317888 similar to PIR|T05693|T056 hypothetical protein F20M13.210 -
Arabidopsis thaliana, partial (13%)
Length = 205
Score = 63.5 bits (153), Expect = 2e-10
Identities = 26/59 (44%), Positives = 37/59 (62%)
Frame = +1
Query: 292 DAMLGFAEKQGIAVRGHNIFWDDPQYQPNWVSSLSPDQLNDAVEKRVNSIVSRYKGQLI 350
D M+ F I RGHNIFW+DP+Y P WV +L+ QL AV R+ ++++YK + I
Sbjct: 25 DQMMQFVRANKIIARGHNIFWEDPKYNPAWVLNLTGTQLKSAVNSRIQRLMNQYKAEFI 201
>AW688565 similar to PIR|D96617|D96 probable xylan endohydrolase isoenzyme
F9K23.10 [imported] - Arabidopsis thaliana, partial
(11%)
Length = 614
Score = 35.8 bits (81), Expect = 0.047
Identities = 27/109 (24%), Positives = 45/109 (40%), Gaps = 10/109 (9%)
Frame = +2
Query: 41 YDYSANVECLAHPQKPQYNGGIIKNPELNDGLQGWTTFGDAIIEHRKSLGNK-------- 92
+ + N +C++ + II NPE DGL WT G I H K
Sbjct: 161 FQKTTNTKCVSTDDE-----NIILNPEFEDGLNNWTGRGCKIAVHESMANGKILPKSGKF 325
Query: 93 FVVTHSRNQPHDSVSQKI--YLRKGLHYSLSAWIQVSEETVPVTAVVKT 139
F R Q + + Q+I +++ L Y ++A +++ V + V T
Sbjct: 326 FASATERTQSWNGIQQEITGRVQRKLAYEVTALVRIFGNNVTTSDVRAT 472
>AW689011 similar to PIR|D96617|D96 probable xylan endohydrolase isoenzyme
F9K23.10 [imported] - Arabidopsis thaliana, partial
(12%)
Length = 591
Score = 33.9 bits (76), Expect = 0.18
Identities = 27/106 (25%), Positives = 43/106 (40%), Gaps = 10/106 (9%)
Frame = +2
Query: 44 SANVECLAHPQKPQYNGGIIKNPELNDGLQGWTTFGDAIIEHRKSLGNK--------FVV 95
+ N +C++ + II NPE DGL WT G I H K F
Sbjct: 164 TTNTKCVSTDDE-----NIILNPEFEDGLNNWTGRGCKIAVHESMANGKILPKSGKFFAS 328
Query: 96 THSRNQPHDSVSQKI--YLRKGLHYSLSAWIQVSEETVPVTAVVKT 139
R Q + + Q+I +++ L Y ++A ++ V + V T
Sbjct: 329 ATERTQSWNGIQQEITGRVQRKLAYEVTALXRIFGNNVTTSDVRAT 466
>TC86597 weakly similar to GP|9837280|gb|AAG00510.1| leaf
senescence-associated receptor-like protein kinase
{Phaseolus vulgaris}, partial (46%)
Length = 2233
Score = 30.8 bits (68), Expect = 1.5
Identities = 19/51 (37%), Positives = 24/51 (46%)
Frame = +3
Query: 240 GFPFGSAINKNILNNNAYQDWFASRFTVTTFENEMKWYTNEYAQGKDNYFD 290
G PF SAI LNN AY + A F ++ TN + KD+ FD
Sbjct: 564 GTPFISAIELRPLNNTAYVTYSAKSVLTNFFRLDVGSITNLEYRYKDDVFD 716
>TC78251 weakly similar to GP|9758571|dbj|BAB09184.1
N-hydroxycinnamoyl/benzoyltransferase-like protein
{Arabidopsis thaliana}, partial (58%)
Length = 1580
Score = 29.6 bits (65), Expect = 3.4
Identities = 14/44 (31%), Positives = 24/44 (53%)
Frame = +2
Query: 218 RAVNEQGHPLPNASISLTMKRPGFPFGSAINKNILNNNAYQDWF 261
+A+N GH +P SI +T G G ++N I + +Y ++F
Sbjct: 458 KAINHDGHTMPLLSIQVTELVDGVFIGCSMNHYIGDGTSYWNFF 589
>BF648593 similar to GP|22770464|gb anthranilate
N-hydroxycinamoyl/bensoiltransferase-like protein {Cicer
arietinum}, partial (78%)
Length = 648
Score = 29.3 bits (64), Expect = 4.4
Identities = 14/44 (31%), Positives = 24/44 (53%)
Frame = +1
Query: 218 RAVNEQGHPLPNASISLTMKRPGFPFGSAINKNILNNNAYQDWF 261
RA+N GH +P SI +T G G ++N I + ++ ++F
Sbjct: 508 RAINHDGHTMPLLSIQVTELGDGVFIGCSMNHCIGDGTSFWNFF 639
>BF521097 similar to GP|22770464|gb| anthranilate
N-hydroxycinamoyl/bensoiltransferase-like protein {Cicer
arietinum}, partial (52%)
Length = 395
Score = 29.3 bits (64), Expect = 4.4
Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 3/69 (4%)
Frame = +1
Query: 218 RAVNEQGHPLPNASISLTMKRPGFPFGSAINKNILNNNAYQDWFASRFTVTTFENEM--- 274
+AVN GH + SI +T G G ++N + + AY ++F + + F+N++
Sbjct: 151 KAVNHDGHTMSLLSIQVTELVDGVFIGCSMNHCLCDGTAYWNFFNTFSEI--FQNDVPAA 324
Query: 275 KWYTNEYAQ 283
+T+ Y Q
Sbjct: 325 SHFTSTYPQ 351
>TC79081 weakly similar to GP|9758571|dbj|BAB09184.1
N-hydroxycinnamoyl/benzoyltransferase-like protein
{Arabidopsis thaliana}, partial (32%)
Length = 913
Score = 29.3 bits (64), Expect = 4.4
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +3
Query: 218 RAVNEQGHPLPNASISLTMKRPGFPFGSAINKNILNNNAYQDWF 261
RAVN GH + SI +T G G ++N + + AY ++F
Sbjct: 435 RAVNHDGHTMSLLSIQVTELLDGVFIGCSMNHAVGDGTAYWNFF 566
>TC77864 weakly similar to PIR|T06329|T06329 symbiotic ammonium transport
protein SAT1 - soybean, partial (21%)
Length = 1411
Score = 28.9 bits (63), Expect = 5.8
Identities = 23/53 (43%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
Frame = +3
Query: 524 LINEWGRAEKSGTTDQNGYFEASLFHGDYE-IEINHPIKKKSNFTHHIKVLSK 575
+IN G A S T + E SLF GDYE +E NH KSN ++ IK L +
Sbjct: 252 IINVEGDAT-SPTNSILSFDETSLFCGDYENVETNH----KSNNSNSIKSLER 395
>TC90412 homologue to SP|P23704|ATPB_NEUCR ATP synthase beta chain
mitochondrial precursor (EC 3.6.3.14). {Neurospora
crassa}, partial (67%)
Length = 1181
Score = 28.9 bits (63), Expect = 5.8
Identities = 14/22 (63%), Positives = 19/22 (85%)
Frame = -3
Query: 518 GDVVDQLINEWGRAEKSGTTDQ 539
GDVVDQL+NE G A+ +GTT++
Sbjct: 153 GDVVDQLLNEDGLAD-TGTTEE 91
>AW587067 similar to PIR|T09584|T09 high mobility group protein HMGI/Y-1 -
sword bean, partial (71%)
Length = 604
Score = 28.5 bits (62), Expect = 7.6
Identities = 21/76 (27%), Positives = 29/76 (37%), Gaps = 3/76 (3%)
Frame = +3
Query: 385 GQTTLFMNEYNTIEDS---RDGLSTPPTYIEKIKEIQSVNSQLPLGIGLESHFPNSPPNL 441
G+ + N Y + + R G PP E + P G + PN PP
Sbjct: 249 GELVFWKNNYTKRDPNAPPRRGRGRPPKAKESFSSGAMFSQPKPRGSPPKD--PNGPPKS 422
Query: 442 PYMRASLDTLRATGLP 457
P M+A + T R G P
Sbjct: 423 PQMKAPVGTGRPRGRP 470
>TC90305 similar to PIR|D96610|D96610 probable integral membrane protein
T8L23.9 [imported] - Arabidopsis thaliana, partial (65%)
Length = 1072
Score = 28.5 bits (62), Expect = 7.6
Identities = 21/52 (40%), Positives = 26/52 (49%)
Frame = +2
Query: 217 VRAVNEQGHPLPNASISLTMKRPGFPFGSAINKNILNNNAYQDWFASRFTVT 268
V A N QGHPLP S +T P+G+ ++ N N Q FA FT T
Sbjct: 335 VVADNIQGHPLPTISAKVTS-----PYGNNLHN---NENVTQGQFA--FTTT 460
>TC79782 weakly similar to GP|11994288|dbj|BAB01471. alanine acetyl
transferase-like protein {Arabidopsis thaliana}, partial
(23%)
Length = 781
Score = 28.5 bits (62), Expect = 7.6
Identities = 15/52 (28%), Positives = 25/52 (47%)
Frame = +1
Query: 453 ATGLPIWITELDVASQPNQALYFEQVLREAHSHPGIQGIVMWTAWSPQGCYR 504
A GLP+W+ + + LR H+ G++ +MW W +GC+R
Sbjct: 472 AKGLPLWL*KK----------WLMLHLRSFHTWRGLRLELMWKMWLLKGCWR 597
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.136 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,181,073
Number of Sequences: 36976
Number of extensions: 269321
Number of successful extensions: 1188
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1184
length of query: 588
length of database: 9,014,727
effective HSP length: 101
effective length of query: 487
effective length of database: 5,280,151
effective search space: 2571433537
effective search space used: 2571433537
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146757.5