BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146756.8 - phase: 0 /pseudo
(469 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein... 125 5e-29
BE941280 weakly similar to GP|20197614|g unknown protein {Arabid... 112 2e-25
BM814840 weakly similar to GP|15128241|db helicase-like protein ... 104 6e-23
BI271341 85 5e-17
CB892155 similar to PIR|D86481|D86 hypothetical protein AAG28292... 80 1e-15
CB892166 similar to GP|20197614|gb unknown protein {Arabidopsis ... 41 9e-04
TC80146 39 0.006
TC84660 similar to PIR|D86481|D86481 hypothetical protein AAG282... 33 0.31
AJ497367 similar to GP|14140286|gb putative helicase {Oryza sati... 32 0.40
TC86840 similar to PIR|H86438|H86438 protein T19E23.7 [imported]... 29 4.4
AW689665 similar to PIR|C84425|C84 similar to axi 1 protein from... 28 9.9
TC87434 similar to SP|P52902|ODPA_PEA Pyruvate dehydrogenase E1 ... 28 9.9
TC77970 similar to GP|20197686|gb|AAM15203.1 expressed protein {... 28 9.9
>TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein T24M8.10 -
Arabidopsis thaliana, partial (11%)
Length = 701
Score = 125 bits (313), Expect = 5e-29
Identities = 67/131 (51%), Positives = 89/131 (67%)
Frame = +3
Query: 225 GKLGEENDGYNDIEISNELLISNFTDPIEAIVQSTYSNLLENFQDGDFLRNRAILASTIQ 284
GK+ E ND Y +++ DPI+AIVQSTY NL+ + + FL++RAIL ST +
Sbjct: 336 GKMSEPNDSYAEVDTPPG-------DPIDAIVQSTYPNLVSQYNNEQFLQSRAILTSTDE 494
Query: 285 VVDDINNYVLNLIPGEEKEYFSSDSIDRSKEDGNDAFEQVTPEFLNCLKTYGLPNHSIIL 344
VVD IN+YVL LIPGEE+ +S+ +RS+ + AF+ + PEFL LKT LPNH + L
Sbjct: 495 VVDQINDYVLKLIPGEERVIYSA---NRSEVNDVQAFDAIPPEFLQSLKTSDLPNHKLTL 665
Query: 345 KVGTPIMLLRN 355
KVGTPIMLLR+
Sbjct: 666 KVGTPIMLLRD 698
>BE941280 weakly similar to GP|20197614|g unknown protein {Arabidopsis
thaliana}, partial (8%)
Length = 403
Score = 112 bits (281), Expect = 2e-25
Identities = 58/120 (48%), Positives = 79/120 (65%)
Frame = -1
Query: 36 LYGYGGIGKTYMWNILAASLRSRKDIVFNVATSGIVALLLPGGRTVHSRFKIPVPTLQSS 95
LY YGG KT++W L+A+LRS +IV A+SGI ALL+PGGRT HSRF IP ++S
Sbjct: 403 LYDYGGTEKTFIWRALSAALRSEGEIVLACASSGIDALLMPGGRTAHSRFGIPFIIDETS 224
Query: 96 ICDIEKGTELAELLKQTKLIIWDEAPMAHKFCFEALYKNH*RIL*V*EENLQNLSLAEKL 155
+C + LA L+ + KLIIWDEAPM HK CFEAL ++ +L +E +++ K+
Sbjct: 223 MCGVTPNIPLASLVIKAKLIIWDEAPMMHKHCFEALDRSLRDVLKTVDERNKDIPFGGKV 44
>BM814840 weakly similar to GP|15128241|db helicase-like protein {Oryza
sativa (japonica cultivar-group)}, partial (6%)
Length = 733
Score = 104 bits (260), Expect = 6e-23
Identities = 57/106 (53%), Positives = 72/106 (67%)
Frame = +3
Query: 364 NGTRLTITRLANHVIEVKIITEKNIGNQVYIPGISMSPSKLPWPFKLVRRQFSIVVSFAI 423
+GTRL I L +VI ++I + G YIP +++ PS R QF +V+SFA+
Sbjct: 27 HGTRLIIVSLGKNVICARVIGGTHAGEVSYIPRMNLIPSGANVSITFERCQFPLVLSFAM 206
Query: 424 TINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGLKILIHD 469
TINKSQGQ+L VGLYLP+ VF+H QLYVA+SRVKS GLKILI D
Sbjct: 207 TINKSQGQTLTSVGLYLPRPVFTHGQLYVAVSRVKSRSGLKILITD 344
>BI271341
Length = 468
Score = 85.1 bits (209), Expect = 5e-17
Identities = 44/76 (57%), Positives = 56/76 (72%), Gaps = 1/76 (1%)
Frame = +1
Query: 188 NHCQVLKLTKNMRLL-NDQAQSSENTERFSE*ILKVGDGKLGEENDGYNDIEISNELLIS 246
+HCQV++L KNM L N Q+ + E+FS+*ILKVGDGK+ E ND Y DI+I ELLIS
Sbjct: 82 DHCQVVRLKKNMWLQQNGQSSNDPEFEQFSK*ILKVGDGKIYEPNDSYADIDIPPELLIS 261
Query: 247 NFTDPIEAIVQSTYSN 262
N+ D ++ IVQSTY N
Sbjct: 262 NYDDSLQTIVQSTYQN 309
>CB892155 similar to PIR|D86481|D86 hypothetical protein AAG28292.1
[imported] - Arabidopsis thaliana, partial (4%)
Length = 572
Score = 80.5 bits (197), Expect = 1e-15
Identities = 40/52 (76%), Positives = 44/52 (83%)
Frame = -2
Query: 416 SIVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGLKILI 467
S+ V FA+TINKSQGQSL H+G+YLP VFSH QLYVALSRV S EGLKILI
Sbjct: 373 SVEVYFAMTINKSQGQSLKHIGVYLPSSVFSHGQLYVALSRVTSREGLKILI 218
>CB892166 similar to GP|20197614|gb unknown protein {Arabidopsis thaliana},
partial (3%)
Length = 748
Score = 41.2 bits (95), Expect = 9e-04
Identities = 19/31 (61%), Positives = 24/31 (77%)
Frame = -1
Query: 439 YLPKDVFSHEQLYVALSRVKSNEGLKILIHD 469
+ ++VFSH QLYVA+SRV S GLKIL+ D
Sbjct: 289 FYDREVFSHGQLYVAISRVSSRNGLKILMID 197
>TC80146
Length = 476
Score = 38.5 bits (88), Expect = 0.006
Identities = 17/44 (38%), Positives = 29/44 (65%)
Frame = -3
Query: 417 IVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSN 460
I + + TINKS+ QSL ++ +YL + +FSHE++Y +K +
Sbjct: 471 IQIPYFKTINKSR*QSLSYMKIYLSRPIFSHEEMYYT*RLIKDH 340
>TC84660 similar to PIR|D86481|D86481 hypothetical protein AAG28292.1
[imported] - Arabidopsis thaliana, partial (1%)
Length = 1009
Score = 32.7 bits (73), Expect = 0.31
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = +2
Query: 437 GLYLPKDVFSHEQLYVALSRVKSNE 461
G+YLP+ +F H LYVALSRV S +
Sbjct: 83 GMYLPQPIF*HG*LYVALSRVTSRK 157
>AJ497367 similar to GP|14140286|gb putative helicase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 543
Score = 32.3 bits (72), Expect = 0.40
Identities = 15/23 (65%), Positives = 20/23 (86%)
Frame = +1
Query: 445 FSHEQLYVALSRVKSNEGLKILI 467
FS+ +LYVA+SRV S +GLKIL+
Sbjct: 19 FSNGKLYVAVSRVTSRKGLKILL 87
>TC86840 similar to PIR|H86438|H86438 protein T19E23.7 [imported] -
Arabidopsis thaliana, partial (34%)
Length = 2940
Score = 28.9 bits (63), Expect = 4.4
Identities = 19/62 (30%), Positives = 28/62 (44%), Gaps = 13/62 (20%)
Frame = +2
Query: 86 KIPVPTLQSSICDIEKGTELAELL---------KQTKLIIW----DEAPMAHKFCFEALY 132
K+ S +CD ++G+ L E T L+IW E P++HK CF+
Sbjct: 1508 KVSTAATISPLCDGQQGSRLQEPQVDC*DQGWHSDTVLLIW*C**RERPISHKSCFKNQC 1687
Query: 133 KN 134
KN
Sbjct: 1688 KN 1693
>AW689665 similar to PIR|C84425|C84 similar to axi 1 protein from Nicotiana
tabacum [imported] - Arabidopsis thaliana, partial (39%)
Length = 675
Score = 27.7 bits (60), Expect = 9.9
Identities = 15/41 (36%), Positives = 27/41 (65%), Gaps = 1/41 (2%)
Frame = -2
Query: 335 YGLPNHSIILKVGTPIMLLRNLD-QAEGLCNGTRLTITRLA 374
Y + +H++ILK+ T +MLL NL + G+ + TR + R++
Sbjct: 458 Y*MDDHALILKL*TFVMLLPNLSIRYSGILSTTRTSFFRIS 336
>TC87434 similar to SP|P52902|ODPA_PEA Pyruvate dehydrogenase E1 component
alpha subunit mitochondrial precursor (EC 1.2.4.1)
(PDHE1-A)., complete
Length = 1829
Score = 27.7 bits (60), Expect = 9.9
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
Frame = -2
Query: 5 FVFSVFMLDEQKNIYNRIMEVVTNEKGGVFFLYGYGGIGKT--YMWN 49
F FSV ++ K+ V NE G F+Y +GG+G ++WN
Sbjct: 229 FDFSVTATEKNKDEDEDFFF*VVNENGEKGFIY*FGGLGVCV*FIWN 89
>TC77970 similar to GP|20197686|gb|AAM15203.1 expressed protein {Arabidopsis
thaliana}, partial (97%)
Length = 2062
Score = 27.7 bits (60), Expect = 9.9
Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 8/61 (13%)
Frame = -1
Query: 5 FVFSVFMLDEQKNIYNRIMEV--------VTNEKGGVFFLYGYGGIGKTYMWNILAASLR 56
F+ ++M+D+ +RI E+ T K L G GGI TY+W I + L
Sbjct: 1960 FITGMYMIDK*NQSNSRIQEMPFSLFLLHCTETKI*EMVLGGEGGIRSTYVWRISPSCLC 1781
Query: 57 S 57
S
Sbjct: 1780 S 1778
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.328 0.144 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,988,940
Number of Sequences: 36976
Number of extensions: 167558
Number of successful extensions: 999
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 993
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 996
length of query: 469
length of database: 9,014,727
effective HSP length: 100
effective length of query: 369
effective length of database: 5,317,127
effective search space: 1962019863
effective search space used: 1962019863
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146756.8