BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146755.4 + phase: 0
(446 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI272968 similar to PIR|T46125|T461 calmodulin-binding heat-shoc... 319 2e-87
TC92309 similar to PIR|T04107|T04107 calmodulin-binding heat-sho... 217 6e-57
BI265725 weakly similar to GP|17065322|gb Unknown protein {Arabi... 169 3e-42
TC84372 similar to GP|15146181|gb|AAK83574.1 AT3g14070/MAG2_2 {A... 67 1e-11
TC91635 similar to PIR|A84854|A84854 hypothetical protein At2g42... 63 2e-10
AL389535 weakly similar to GP|21428982|gb GH28840p {Drosophila m... 33 0.17
BG584241 weakly similar to GP|20146425|db lipase-like protein {O... 33 0.29
BF648646 similar to GP|20161014|db hypothetical protein~similar ... 32 0.38
TC80178 similar to GP|4512677|gb|AAD21731.1| unknown protein {Ar... 31 0.84
TC78175 similar to GP|1527001|gb|AAB07724.1| Ipomoea nil Pn47p, ... 30 1.4
TC87585 similar to GP|22655024|gb|AAM98103.1 At1g02660/T14P4_9 {... 29 3.2
TC77094 weakly similar to PIR|A96608|A96608 hypothetical protein... 29 3.2
TC91928 similar to GP|20197590|gb|AAD14526.2 predicted by gensca... 28 7.1
TC90576 similar to GP|9758236|dbj|BAB08735.1 gb|AAD29063.1~gene_... 28 7.1
BM815269 similar to GP|9758132|dbj| gene_id:MUD21.8~unknown prot... 28 9.3
AW774193 similar to PIR|B96625|B96 hypothetical protein T2K10.12... 28 9.3
TC86439 similar to GP|15209172|gb|AAK91890.1 putative amidase {S... 28 9.3
>BI272968 similar to PIR|T46125|T461 calmodulin-binding heat-shock-like
protein - Arabidopsis thaliana, partial (16%)
Length = 550
Score = 319 bits (817), Expect = 2e-87
Identities = 157/160 (98%), Positives = 157/160 (98%)
Frame = +3
Query: 287 RLYHIVERKPFRLGRFPPVVRTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAQRALDLMM 346
RLYHIVERKPFRLGRFPPVVRTAVPVDGRFEHIVLSCNA DHAIIWIEKEAQRALDLMM
Sbjct: 3 RLYHIVERKPFRLGRFPPVVRTAVPVDGRFEHIVLSCNAXXDHAIIWIEKEAQRALDLMM 182
Query: 347 EKDNTMEVPAKQIMQRQKTMTRHGQEYKAALQRAKTLDIPHAFTPPSEYGTFDEEGEESS 406
EKDNTMEVPAKQIMQRQKTMTRHGQEYKAALQRAKTLDIPHAFTPPSEYGTFDEEGEESS
Sbjct: 183 EKDNTMEVPAKQIMQRQKTMTRHGQEYKAALQRAKTLDIPHAFTPPSEYGTFDEEGEESS 362
Query: 407 RSEAESSVSSTNRSTVNESWDVLIERLFDKDEHGHMVLKR 446
RS AESSVSSTNRSTVNESWDVLIERLFDKDEHGHMVLKR
Sbjct: 363 RSXAESSVSSTNRSTVNESWDVLIERLFDKDEHGHMVLKR 482
>TC92309 similar to PIR|T04107|T04107 calmodulin-binding heat-shock protein
- common tobacco, partial (36%)
Length = 763
Score = 217 bits (553), Expect = 6e-57
Identities = 99/164 (60%), Positives = 124/164 (75%), Gaps = 1/164 (0%)
Frame = +1
Query: 1 MSIICGLPLVECVYCLACVRWAWKRCLHTAGHDSETWGFAATQEFEPVPRLCRYILAVYE 60
MS+ CG ECV L C RW WKRC + DS TW A EFEPVPR+CR ILA+YE
Sbjct: 250 MSVACG---AECVLVLGCARWLWKRCTYIGADDSATWPPATAVEFEPVPRVCRIILAIYE 420
Query: 61 DDLRNPLWA-PPGGYGINPDWLLLRKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAR 119
DLRNP A P GY +NPDW++ R TY++T G APPYI+Y+DHDH +IV+A+RGLNLA+
Sbjct: 421 PDLRNPKLAIPANGYRLNPDWVIKRVTYEETEGHAPPYIIYVDHDHREIVMAVRGLNLAK 600
Query: 120 ESDYAVLLDNKLGKRKFDGGYVHNGLLKAAGWVMDAECEILREL 163
ESDY +LLDNKLG ++FDGGYVH+GLLK+A W+++ E E L++L
Sbjct: 601 ESDYKLLLDNKLGMQRFDGGYVHHGLLKSAVWLLNRESETLKKL 732
>BI265725 weakly similar to GP|17065322|gb Unknown protein {Arabidopsis
thaliana}, partial (22%)
Length = 655
Score = 169 bits (427), Expect = 3e-42
Identities = 68/104 (65%), Positives = 84/104 (80%)
Frame = +1
Query: 1 MSIICGLPLVECVYCLACVRWAWKRCLHTAGHDSETWGFAATQEFEPVPRLCRYILAVYE 60
MS ICGLP++ECVYCL C RWAWK+CL+TAG++SE WG A +EFEP+PR+CR ILAVY+
Sbjct: 340 MSFICGLPILECVYCLGCTRWAWKKCLYTAGYESENWGLATAEEFEPIPRICRTILAVYQ 519
Query: 61 DDLRNPLWAPPGGYGINPDWLLLRKTYKDTRGRAPPYILYLDHD 104
DD+RNP WAP GYGINPDW++L K Y D + P ++YLDHD
Sbjct: 520 DDIRNPQWAPEXGYGINPDWIVLXKDYGDNQRCVTPDMIYLDHD 651
>TC84372 similar to GP|15146181|gb|AAK83574.1 AT3g14070/MAG2_2 {Arabidopsis
thaliana}, partial (35%)
Length = 755
Score = 67.4 bits (163), Expect = 1e-11
Identities = 46/165 (27%), Positives = 77/165 (45%), Gaps = 10/165 (6%)
Frame = +1
Query: 95 PPYILYLDHDHADIVLAIRGLNLARESDYAVL---------LDNKLGKRKFDGGYVHNGL 145
P + + +DH +L IRG + +++ AV + ++ G GY H G+
Sbjct: 157 PAFTIIVDHKMRCFLLLIRGTHSIKDTLTAVTGSVVPFHHTVVHQGGVSDLVLGYAHCGM 336
Query: 146 LKAAGWVMDAECEILRELVEKYPNYTLTFAGHSLGSGVAAALSMVVVQNRDRLGNIERKR 205
+ AA W+ L + + +YP+Y + GHSLG G AA L+ V+ + + E
Sbjct: 337 VAAARWIAKLATPCLLDALGQYPDYEVKIVGHSLGGGTAAILTYVLREQK------ELSV 498
Query: 206 VRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLPR-TATPLEDI 249
C APA CM+ LA I S++ D +P +A ++D+
Sbjct: 499 TTCVTFAPAACMTWELAESGNSFITSIINGADLVPTFSAASVDDL 633
>TC91635 similar to PIR|A84854|A84854 hypothetical protein At2g42450
[imported] - Arabidopsis thaliana, partial (71%)
Length = 1919
Score = 63.2 bits (152), Expect = 2e-10
Identities = 37/147 (25%), Positives = 70/147 (47%)
Frame = +1
Query: 95 PPYILYLDHDHADIVLAIRGLNLARESDYAVLLDNKLGKRKFDGGYVHNGLLKAAGWVMD 154
P Y + +D ++L IRG + + ++ + G+ ++G H G + A W +
Sbjct: 709 PAYYIGVDPRKKLVILGIRGTHALYDL-ITDIVSSSDGEVTYEGYSTHFGTAECARWFLH 885
Query: 155 AECEILRELVEKYPNYTLTFAGHSLGSGVAAALSMVVVQNRDRLGNIERKRVRCYAIAPA 214
E +R+ ++K+ + L GHSLG +A+ L++++ + + V
Sbjct: 886 HEIGNIRKYLQKHEGFRLRLVGHSLGGAIASLLAIMIHRKSSKELGFSPDIVSAVGYGTP 1065
Query: 215 RCMSLNLAVRYADVINSVVLQDDFLPR 241
C+S LA A + +VV+QDD +PR
Sbjct: 1066PCVSKELAESCAGYVTTVVMQDDIVPR 1146
>AL389535 weakly similar to GP|21428982|gb GH28840p {Drosophila
melanogaster}, partial (1%)
Length = 433
Score = 33.5 bits (75), Expect = 0.17
Identities = 16/62 (25%), Positives = 35/62 (55%), Gaps = 3/62 (4%)
Frame = +2
Query: 380 AKTLDIPHAFTPPSEYGTFDEEGEESSRSEAESSVSSTNRSTVNE---SWDVLIERLFDK 436
A +L++PHA + + D EGE +S S + +S++ S + +W+ ++E+L +
Sbjct: 2 AVSLNVPHAVDTVEKEPSEDNEGEAASGSSGDKEAASSSESKPSGGKLNWEEVVEKLLEN 181
Query: 437 DE 438
++
Sbjct: 182 EK 187
>BG584241 weakly similar to GP|20146425|db lipase-like protein {Oryza sativa
(japonica cultivar-group)}, partial (55%)
Length = 818
Score = 32.7 bits (73), Expect = 0.29
Identities = 16/37 (43%), Positives = 24/37 (64%), Gaps = 2/37 (5%)
Frame = +3
Query: 160 LRELVEKYPN--YTLTFAGHSLGSGVAAALSMVVVQN 194
+R LVEKY N ++T GHSLG+ +A ++ +V N
Sbjct: 270 VRRLVEKYKNEEISITITGHSLGAAIATLNAVDIVTN 380
>BF648646 similar to GP|20161014|db hypothetical protein~similar to
PK1-related protein kinase 2 {Oryza sativa (japonica
cultivar-group), partial (24%)
Length = 655
Score = 32.3 bits (72), Expect = 0.38
Identities = 20/69 (28%), Positives = 33/69 (46%)
Frame = +1
Query: 170 YTLTFAGHSLGSGVAAALSMVVVQNRDRLGNIERKRVRCYAIAPARCMSLNLAVRYADVI 229
Y + GHSLG +AA L + Q +R N+ Y+ P C+ L +A + I
Sbjct: 463 YNVRIVGHSLGGAIAALLGL---QLYNRYPNL-----HVYSYGPLPCVDLVVANACSSFI 618
Query: 230 NSVVLQDDF 238
S++ ++F
Sbjct: 619 TSIIYGNEF 645
>TC80178 similar to GP|4512677|gb|AAD21731.1| unknown protein {Arabidopsis
thaliana}, partial (17%)
Length = 946
Score = 31.2 bits (69), Expect = 0.84
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +3
Query: 400 EEGEESSRSEAESSVSSTNRSTVNESWDVLIER 432
EEGE+ + + +V S N+ ++E+WDV +R
Sbjct: 606 EEGEDEEHKKIDENVLSDNKPYLSEAWDVFDQR 704
>TC78175 similar to GP|1527001|gb|AAB07724.1| Ipomoea nil Pn47p, partial
(32%)
Length = 1153
Score = 30.4 bits (67), Expect = 1.4
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = +1
Query: 160 LRELVEKYPN--YTLTFAGHSLGSGVAAALSMVVVQNR 195
+ LVE Y N ++T GHSLG +A SM +V N+
Sbjct: 691 ISRLVELYKNEEISITVTGHSLGGALATISSMDIVANK 804
>TC87585 similar to GP|22655024|gb|AAM98103.1 At1g02660/T14P4_9 {Arabidopsis
thaliana}, partial (53%)
Length = 1666
Score = 29.3 bits (64), Expect = 3.2
Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Frame = +3
Query: 135 KFDG--GYVHNGLLKAA-GWVMDAECEILRELVEKYPNYTLTFAGHSLGSGVAAALSMVV 191
KF+G VH G+ +AA G + E+ L + T F GHSLG +A +++++
Sbjct: 420 KFEGLDVLVHRGIYEAAKGIYLQMLPEVHAHLKSRGSRATFRFTGHSLGGSLALLVNLML 599
>TC77094 weakly similar to PIR|A96608|A96608 hypothetical protein F25P12.93
[imported] - Arabidopsis thaliana, partial (57%)
Length = 1745
Score = 29.3 bits (64), Expect = 3.2
Identities = 36/150 (24%), Positives = 57/150 (38%), Gaps = 25/150 (16%)
Frame = +1
Query: 74 YGINPDWLL----LRKTYKDTRGRAPPYILYL---DHDHADIVLAIRGLNLARESDYAVL 126
Y I DW + + + + RA +L L D V+A RG +
Sbjct: 559 YTIKYDWKMEYVGFFDCWNEYQERATTQVLILLDKFKDRDTYVVAFRGTEPFDADAWCTD 738
Query: 127 LDNKL----GKRKFDGGYVHN-GLLKAAGWVMDAECE-------------ILRELVEKYP 168
LD G + GG++ GL K GW + E + ILR+ + + P
Sbjct: 739 LDISWYGIPGVGRAHGGFMKALGLQKNLGWPKEIERDEKLAPLAYYVIRDILRKGLSENP 918
Query: 169 NYTLTFAGHSLGSGVAAALSMVVVQNRDRL 198
N GHSLG +A ++ + ++L
Sbjct: 919 NAKFIITGHSLGGALAILFPTIMFLHDEKL 1008
>TC91928 similar to GP|20197590|gb|AAD14526.2 predicted by genscan and
genefinder {Arabidopsis thaliana}, partial (30%)
Length = 611
Score = 28.1 bits (61), Expect = 7.1
Identities = 10/31 (32%), Positives = 16/31 (51%)
Frame = +3
Query: 44 EFEPVPRLCRYILAVYEDDLRNPLWAPPGGY 74
E + VP++ + + + PLW P GGY
Sbjct: 351 EIDGVPKMSLPVFGLASYKFKGPLWTPSGGY 443
>TC90576 similar to GP|9758236|dbj|BAB08735.1
gb|AAD29063.1~gene_id:K3K7.3~similar to unknown protein
{Arabidopsis thaliana}, partial (55%)
Length = 933
Score = 28.1 bits (61), Expect = 7.1
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +3
Query: 158 EILRELVEKYPNYTLTFAGHSLGSGVA 184
E L+ + E Y + + AGHSLG+G A
Sbjct: 564 ETLKSICETYGSSNVCIAGHSLGAGFA 644
>BM815269 similar to GP|9758132|dbj| gene_id:MUD21.8~unknown protein
{Arabidopsis thaliana}, partial (3%)
Length = 682
Score = 27.7 bits (60), Expect = 9.3
Identities = 13/21 (61%), Positives = 14/21 (65%)
Frame = -3
Query: 241 RTATPLEDIFKSLFCLPCLLC 261
R ATP DI LFC+PCL C
Sbjct: 590 RMATP--DIICILFCMPCLWC 534
>AW774193 similar to PIR|B96625|B96 hypothetical protein T2K10.12 [imported]
- Arabidopsis thaliana, partial (21%)
Length = 561
Score = 27.7 bits (60), Expect = 9.3
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -1
Query: 12 CVYCLACVRWAWKRCL 27
C+ L C+ AWKRCL
Sbjct: 144 CIMLLQCIHEAWKRCL 97
>TC86439 similar to GP|15209172|gb|AAK91890.1 putative amidase {Solanum
demissum}, partial (54%)
Length = 1791
Score = 27.7 bits (60), Expect = 9.3
Identities = 8/14 (57%), Positives = 9/14 (64%)
Frame = +1
Query: 15 CLACVRWAWKRCLH 28
CL C RW WK +H
Sbjct: 532 CLECQRWTWKESIH 573
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.139 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,187,398
Number of Sequences: 36976
Number of extensions: 230132
Number of successful extensions: 1149
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1146
length of query: 446
length of database: 9,014,727
effective HSP length: 99
effective length of query: 347
effective length of database: 5,354,103
effective search space: 1857873741
effective search space used: 1857873741
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146755.4