BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146752.15 + phase: 0 /pseudo
(178 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ139175 weakly similar to GP|22136396|gb| putative protein {Ara... 146 4e-36
TC85286 similar to GP|3776572|gb|AAC64889.1| ESTs gb|R65052 gb|... 32 0.17
TC91388 similar to GP|14334724|gb|AAK59540.1 unknown protein {Ar... 31 0.30
TC85922 similar to SP|P56807|RR18_ARATH Chloroplast 30S ribosoma... 30 0.39
TC80920 similar to GP|21553830|gb|AAM62923.1 oligouridylate bind... 30 0.66
AW257005 homologue to GP|18144465|dbj cyclomaltodextrinase {Clos... 29 0.86
TC82255 similar to GP|6692095|gb|AAF24560.1| F22C12.5 {Arabidops... 29 1.1
TC87983 similar to SP|Q10128|YSO2_CAEEL Hypothetical 81.6 kDa pr... 29 1.1
TC90505 similar to GP|3941528|gb|AAC83640.1| putative transcript... 28 1.5
BE204888 similar to GP|21595622|gb| unknown {Arabidopsis thalian... 28 1.9
TC80251 homologue to GP|20975734|emb|CAD29823. putative ubiquiti... 28 2.5
BQ137274 GP|12053007|e hypothetical protein {Homo sapiens}, part... 28 2.5
TC77200 similar to GP|22597162|gb|AAN03468.1 bZIP transcription ... 28 2.5
TC88329 similar to PIR|T47827|T47827 squamosa promoter binding p... 28 2.5
TC88718 weakly similar to GP|3850569|gb|AAC72109.1| ESTs gb|T212... 27 3.3
TC87692 similar to GP|21537190|gb|AAM61531.1 putative SKP1-like ... 27 3.3
TC85738 similar to GP|6091551|gb|AAF01764.2| homeodomain-leucine... 27 5.6
TC85898 similar to GP|12324950|gb|AAG52429.1 putative aminopepti... 27 5.6
BG585187 26 7.3
TC76434 similar to GP|7715089|gb|AAF67863.1| cyclopropyl isomera... 26 7.3
>BQ139175 weakly similar to GP|22136396|gb| putative protein {Arabidopsis
thaliana}, partial (49%)
Length = 661
Score = 146 bits (369), Expect = 4e-36
Identities = 80/113 (70%), Positives = 87/113 (76%), Gaps = 4/113 (3%)
Frame = +2
Query: 70 FLVKLLLRLPVIKVLKLILVILTKLSFSCRVCCDYASFWSTA*----DSLYEPVLLECVT 125
FLVKLLLRLPVIK +I+ +F ++ Y S T D + +PVLLECVT
Sbjct: 2 FLVKLLLRLPVIKESVVIMP-----AFGVQLETHYMSGKVTRRFVPIDRILKPVLLECVT 166
Query: 126 PVTCYWTLSLIVREESEMVLVYKNLRPPVKMLVHVWKALCAATDNKGETCIHA 178
PVTCYWTLSLIVREESEMVLVYKNLRPPVKMLVHVWKALCAATDNKGETCIHA
Sbjct: 167 PVTCYWTLSLIVREESEMVLVYKNLRPPVKMLVHVWKALCAATDNKGETCIHA 325
>TC85286 similar to GP|3776572|gb|AAC64889.1| ESTs gb|R65052 gb|AA712146
gb|H76533 gb|H76282 gb|AA650771 gb|H76287
gb|AA650887 gb|N37383, partial (77%)
Length = 1947
Score = 31.6 bits (70), Expect = 0.17
Identities = 23/71 (32%), Positives = 33/71 (46%)
Frame = -1
Query: 33 YFFVYASALIVLACGIYLYLLEEKSISLVYYSLLFDIFLVKLLLRLPVIKVLKLILVILT 92
Y Y S + +++ I YLL SL Y L++ FL+ L + L L+
Sbjct: 375 YIDTYISLIFLVS*YISYYLLSVTFASLFLYILIYLSFLLSFFALLYLCFFLFLLF---- 208
Query: 93 KLSFSCRVCCD 103
LS S R+CCD
Sbjct: 207 -LSQSARLCCD 178
>TC91388 similar to GP|14334724|gb|AAK59540.1 unknown protein {Arabidopsis
thaliana}, partial (14%)
Length = 691
Score = 30.8 bits (68), Expect = 0.30
Identities = 23/89 (25%), Positives = 48/89 (53%), Gaps = 2/89 (2%)
Frame = +2
Query: 21 IHHIVLRSNGGKYFFVYASALIVL-ACGIYLYLLEEKSISLVYYSLLFDIFLVKLLLRLP 79
+H++ + S G + V+++++ VL A + +YL+ E + Y FL+ L+L +P
Sbjct: 425 LHNVGVESTGTVSWTVFSASIFVLVALVLSMYLIFEHLAA--YNQPEEQKFLIGLILMVP 598
Query: 80 VIKVLKLILVILTKLSFSCRVCCD-YASF 107
V + + ++ + +F+C V D Y +F
Sbjct: 599 VYALESFLSLLDSSAAFNCEVIRDCYEAF 685
>TC85922 similar to SP|P56807|RR18_ARATH Chloroplast 30S ribosomal protein
S18. [Mouse-ear cress] {Arabidopsis thaliana}, partial
(74%)
Length = 3196
Score = 30.4 bits (67), Expect = 0.39
Identities = 15/59 (25%), Positives = 31/59 (52%)
Frame = -3
Query: 33 YFFVYASALIVLACGIYLYLLEEKSISLVYYSLLFDIFLVKLLLRLPVIKVLKLILVIL 91
YF +Y +++I++ IYLY+L YY+L ++V + + P+ + + I+
Sbjct: 1733 YFLLYFNSIILMLLYIYLYILYSLCSCYYYYTL----YIVNIYIIYPIYSSISYLYDII 1569
>TC80920 similar to GP|21553830|gb|AAM62923.1 oligouridylate binding protein
putative {Arabidopsis thaliana}, partial (41%)
Length = 694
Score = 29.6 bits (65), Expect = 0.66
Identities = 16/42 (38%), Positives = 27/42 (64%)
Frame = -3
Query: 56 KSISLVYYSLLFDIFLVKLLLRLPVIKVLKLILVILTKLSFS 97
K SL+++SLL + L+ LLL P+I +L+L++L F+
Sbjct: 212 KKSSLLHHSLLLLLMLMLLLLHQPMILHFQLLLLLLPSEIFT 87
>AW257005 homologue to GP|18144465|dbj cyclomaltodextrinase {Clostridium
perfringens str. 13}, partial (1%)
Length = 355
Score = 29.3 bits (64), Expect = 0.86
Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 5/54 (9%)
Frame = -1
Query: 32 KYFFVYASALIVLACGIYLYLLEEKSI-----SLVYYSLLFDIFLVKLLLRLPV 80
KYFF + I+ AC + EEK I S +++ ++ IFL LL LP+
Sbjct: 283 KYFFFFFPFYIIYAC-----I*EEKEIFNSLFSFLFFIIICKIFLFLLLFFLPI 137
>TC82255 similar to GP|6692095|gb|AAF24560.1| F22C12.5 {Arabidopsis
thaliana}, partial (50%)
Length = 836
Score = 28.9 bits (63), Expect = 1.1
Identities = 16/33 (48%), Positives = 24/33 (72%), Gaps = 4/33 (12%)
Frame = +3
Query: 65 LLFDIFLVKLLLRLPVIKVL----KLILVILTK 93
++F IFL +LLLRL ++ VL K++LVIL +
Sbjct: 543 VIFKIFLPRLLLRLKMVLVLLTLVKVVLVILLR 641
>TC87983 similar to SP|Q10128|YSO2_CAEEL Hypothetical 81.6 kDa protein
F56D1.2 in chromosome II precursor. {Caenorhabditis
elegans}, partial (2%)
Length = 745
Score = 28.9 bits (63), Expect = 1.1
Identities = 19/67 (28%), Positives = 28/67 (41%), Gaps = 3/67 (4%)
Frame = -3
Query: 39 SALIVLACGIYLYLLEEKSISLVYYSL---LFDIFLVKLLLRLPVIKVLKLILVILTKLS 95
S ++ C Y + + + ++ + LF LLL +P I L L L I
Sbjct: 695 SEILAFICPYYFGYNNDIKL*ICFHLIK*TLFSCVASHLLLLVPCINSLALSLDISFHCH 516
Query: 96 FSCRVCC 102
F CR CC
Sbjct: 515 FRCRCCC 495
>TC90505 similar to GP|3941528|gb|AAC83640.1| putative transcription factor
{Arabidopsis thaliana}, partial (49%)
Length = 984
Score = 28.5 bits (62), Expect = 1.5
Identities = 13/41 (31%), Positives = 25/41 (60%)
Frame = -1
Query: 32 KYFFVYASALIVLACGIYLYLLEEKSISLVYYSLLFDIFLV 72
K+FF +A ++ C +L LL + SI +V+Y ++ ++ V
Sbjct: 561 KFFFFKINAFSIITCL*FL*LLFQMSIPIVFYVIICSLWKV 439
>BE204888 similar to GP|21595622|gb| unknown {Arabidopsis thaliana}, partial
(16%)
Length = 632
Score = 28.1 bits (61), Expect = 1.9
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 7/64 (10%)
Frame = +2
Query: 46 CGIYLYLLEEKSISLVYYSLLFD-------IFLVKLLLRLPVIKVLKLILVILTKLSFSC 98
C + LL K + V Y LF+ +FL+ L ++ +K KL ++ T+ ++C
Sbjct: 326 CTLTCCLLYLKLVIFVVYLNLFN*YIACLKVFLLFCLTKVICMKATKLTTLLYTRAVWAC 505
Query: 99 RVCC 102
R C
Sbjct: 506 RTRC 517
>TC80251 homologue to GP|20975734|emb|CAD29823. putative
ubiquitin-conjugating enzyme {Populus euramericana},
partial (96%)
Length = 893
Score = 27.7 bits (60), Expect = 2.5
Identities = 20/62 (32%), Positives = 29/62 (46%), Gaps = 4/62 (6%)
Frame = +2
Query: 8 YVHDQKYPSEDVDIHHIVLRSNGGKYFFVYASALIVLACGI----YLYLLEEKSISLVYY 63
Y H +KYPS ++H +L G K + A L + C + Y YLL L+Y
Sbjct: 641 YAHFRKYPSMAAVVYHFLL---GAK---IVA*LLTAMTCRLFGFDYFYLLSGLDNMLIYD 802
Query: 64 SL 65
+L
Sbjct: 803 NL 808
>BQ137274 GP|12053007|e hypothetical protein {Homo sapiens}, partial (1%)
Length = 863
Score = 27.7 bits (60), Expect = 2.5
Identities = 18/58 (31%), Positives = 31/58 (53%)
Frame = -3
Query: 33 YFFVYASALIVLACGIYLYLLEEKSISLVYYSLLFDIFLVKLLLRLPVIKVLKLILVI 90
+F++Y L C IYL L SLVYY LL + ++ +L L ++ + I+++
Sbjct: 861 FFYIY------LLCYIYL*YLILIYFSLVYYFLLCSL*ILSILYVLFILYYIIYIILV 706
>TC77200 similar to GP|22597162|gb|AAN03468.1 bZIP transcription factor ATB2
{Glycine max}, partial (84%)
Length = 1481
Score = 27.7 bits (60), Expect = 2.5
Identities = 22/60 (36%), Positives = 34/60 (56%), Gaps = 12/60 (20%)
Frame = +2
Query: 40 ALIVLACGIYLYLLE----EKSISLVYYSLLFDIF--------LVKLLLRLPVIKVLKLI 87
+LIV IY+Y+++ S L++ SLLF+IF + LLL + +IK +KLI
Sbjct: 341 SLIV*TYIIYIYIIKIIKFYISAVLIFLSLLFNIFYSVWVLLVFLSLLLLVLIIKRVKLI 520
>TC88329 similar to PIR|T47827|T47827 squamosa promoter binding protein-like
12 [imported] - Arabidopsis thaliana, partial (19%)
Length = 1277
Score = 27.7 bits (60), Expect = 2.5
Identities = 25/75 (33%), Positives = 38/75 (50%), Gaps = 2/75 (2%)
Frame = +3
Query: 33 YFFVYASALIVLACGIYLYLLEEKSISLVYYSLLFDIFLVKLLLRLPVIKVLKLILV--I 90
Y F+Y + L +LL+ K+I + Y LL FLV+L + + + +LKL L +
Sbjct: 1044 YAFIYWLNWVFL-----FFLLKLKTIMVFTYLLLIS-FLVRLHVWICLYCILKLHLFKSL 1205
Query: 91 LTKLSFSCRVCCDYA 105
L + RVCC A
Sbjct: 1206 LLLYNSIIRVCCSAA 1250
>TC88718 weakly similar to GP|3850569|gb|AAC72109.1| ESTs gb|T21276
gb|T45403 and gb|AA586113 come from this gene.
{Arabidopsis thaliana}, partial (16%)
Length = 1356
Score = 27.3 bits (59), Expect = 3.3
Identities = 9/21 (42%), Positives = 14/21 (65%)
Frame = -1
Query: 4 TRYNYVHDQKYPSEDVDIHHI 24
TR++Y H + PS D +HH+
Sbjct: 927 TRHHYYHHHQNPSSDPPLHHL 865
>TC87692 similar to GP|21537190|gb|AAM61531.1 putative SKP1-like protein
{Arabidopsis thaliana}, partial (50%)
Length = 1539
Score = 27.3 bits (59), Expect = 3.3
Identities = 18/66 (27%), Positives = 33/66 (49%)
Frame = -2
Query: 22 HHIVLRSNGGKYFFVYASALIVLACGIYLYLLEEKSISLVYYSLLFDIFLVKLLLRLPVI 81
H I + + G K F + + + + IYLY+L + L ++ L F + LLR+ +
Sbjct: 1055 HKITM*TRGLKRFICFRTLVSDVVMAIYLYIL----LMLAFFPLFFILRSSFDLLRITIN 888
Query: 82 KVLKLI 87
K K++
Sbjct: 887 KRQKVV 870
>TC85738 similar to GP|6091551|gb|AAF01764.2| homeodomain-leucine zipper
protein 56 {Glycine max}, partial (77%)
Length = 2144
Score = 26.6 bits (57), Expect = 5.6
Identities = 15/51 (29%), Positives = 27/51 (52%)
Frame = -2
Query: 34 FFVYASALIVLACGIYLYLLEEKSISLVYYSLLFDIFLVKLLLRLPVIKVL 84
F + S ++LACG+Y+ LL K L++IF ++ L +++ L
Sbjct: 229 FLGFGSLFLLLACGVYV*LLNIK---------LYEIFAKQIALNENLLETL 104
>TC85898 similar to GP|12324950|gb|AAG52429.1 putative aminopeptidase;
4537-10989 {Arabidopsis thaliana}, partial (90%)
Length = 3374
Score = 26.6 bits (57), Expect = 5.6
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = +3
Query: 96 FSCRVCCDYASFWS 109
F CR+CC+ S+W+
Sbjct: 1185 FRCRLCCNIGSYWT 1226
>BG585187
Length = 685
Score = 26.2 bits (56), Expect = 7.3
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +3
Query: 115 LYEPVLLECVTPVTCYWTLSLIVREE 140
LY +L C T +TCY + ++ EE
Sbjct: 90 LYATILT*CTTQITCYRCMIILTYEE 167
>TC76434 similar to GP|7715089|gb|AAF67863.1| cyclopropyl isomerase
{Arabidopsis thaliana}, partial (49%)
Length = 516
Score = 26.2 bits (56), Expect = 7.3
Identities = 21/76 (27%), Positives = 40/76 (52%), Gaps = 1/76 (1%)
Frame = +2
Query: 10 HDQKYPSEDVDIHHIVLRSNGGKYFFVYASALIVLACGIYLYLLEEKSISLVYYSLLFD- 68
H K+ S + HH + RS G + ++ +LACG++ +++ + S + +LLF
Sbjct: 11 HYIKFRSYLISSHHQI-RSIGSPKWALHQ----LLACGLHQISVKDGANSSFFSTLLFGF 175
Query: 69 IFLVKLLLRLPVIKVL 84
+FL+ LL ++L
Sbjct: 176LFLLALLFLTSFTRIL 223
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.331 0.144 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,255,938
Number of Sequences: 36976
Number of extensions: 119793
Number of successful extensions: 1017
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 1006
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1014
length of query: 178
length of database: 9,014,727
effective HSP length: 90
effective length of query: 88
effective length of database: 5,686,887
effective search space: 500446056
effective search space used: 500446056
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146752.15