BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146743.7 + phase: 0
(376 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79701 homologue to PIR|T06242|T06242 aspartate kinase (EC 2.7.... 126 4e-38
BI308719 similar to PIR|T06242|T06 aspartate kinase (EC 2.7.2.4)... 100 7e-22
TC87813 similar to SP|P49299|CYSZ_CUCMA Citrate synthase glyoxy... 29 2.6
AJ388848 similar to GP|20805122|db protein kinase -like {Oryza s... 27 9.8
TC87927 weakly similar to GP|12321870|gb|AAG50966.1 unknown prot... 27 9.8
BG454627 similar to GP|15777895|gb SKIP5-like protein {Lycopersi... 27 9.8
BE998520 27 9.8
>TC79701 homologue to PIR|T06242|T06242 aspartate kinase (EC 2.7.2.4) /
homoserine dehydrogenase (EC 1.1.1.3) precursor -
soybean, partial (28%)
Length = 1042
Score = 126 bits (316), Expect(2) = 4e-38
Identities = 70/167 (41%), Positives = 104/167 (61%), Gaps = 2/167 (1%)
Frame = +1
Query: 205 EDGKPLSQVVRAAKSLGYTEPDPRDDLGGMDVARKALILARILGRRINMDSIQIESLYPK 264
+DG+ S+VV AK GYTEPDPRDDL G DVARK +ILAR G ++ + +I IESL P+
Sbjct: 253 KDGRAFSEVVGEAKEAGYTEPDPRDDLSGTDVARKVIILARESGLKLELSNIPIESLVPE 432
Query: 265 EMGPDVMTDDDFLSCGLLLLDKDIQERVEKAASNGNVLRYVCVIE--GPRCEVGIQELPK 322
+ + +F+ L D++ ++ E A + G VLRYV V++ + V +++ K
Sbjct: 433 PLRA-CASAQEFMQ-QLPKFDQEFAKKQEDADNAGEVLRYVGVVDVTNKKGVVELRKYKK 606
Query: 323 NSALGRLRGSDNVLEVYTRCYSNQPLVIQGAGAGNDTTAAGVLADIV 369
+ +L GSDN++ TR Y NQPL+++G GAG TA G+ +DI+
Sbjct: 607 DHPFAQLSGSDNIIAFTTRRYKNQPLIVRGPGAGAQVTAGGIFSDIL 747
Score = 49.7 bits (117), Expect(2) = 4e-38
Identities = 26/75 (34%), Positives = 44/75 (58%), Gaps = 4/75 (5%)
Frame = +3
Query: 132 GCCVVMANKKPLTSTMGDFEKLLTYPRRIR----HESTVGAGLPVISSLNRIISSGDPVN 187
G V+ NKK + + + +L R+ +E+TVGAGLP++S+L ++ +GD +
Sbjct: 21 GIHVITPNKKANSGPLEQYLRLRALQRQSYTHYFYEATVGAGLPIVSTLRGLLETGDKIL 200
Query: 188 RIIGSLSGTLGYVMS 202
+I G SGTL Y+ +
Sbjct: 201 QIEGIFSGTLSYIFN 245
>BI308719 similar to PIR|T06242|T06 aspartate kinase (EC 2.7.2.4) /
homoserine dehydrogenase (EC 1.1.1.3) precursor -
soybean, partial (21%)
Length = 591
Score = 100 bits (250), Expect = 7e-22
Identities = 70/213 (32%), Positives = 107/213 (49%), Gaps = 4/213 (1%)
Frame = +1
Query: 27 SLHSSQGLCLRVVGIGDSKSLVVVDDLLNKGFDDSFLLELCRLKHGGESLSKLGDLGQCQ 86
+L + LRV+GI SKS+++ D ++L + + E ++ DL +
Sbjct: 19 TLKEESNIDLRVMGIMGSKSMLL----------DDLGIDLVKWREIREEKGEVADLEKFV 168
Query: 87 VFVHPESEGKILEIASQLGKKTGLAFVDCTASSDTIVVLKQVVDLGCCVVMANKKPLTST 146
VH + + T L VDCTA S + G V+ NKK +
Sbjct: 169 QHVH----------GNHVIPNTVL--VDCTADSVIASHYDDWLRKGIHVITPNKKANSGP 312
Query: 147 MGDFEKLLTYPRRIR----HESTVGAGLPVISSLNRIISSGDPVNRIIGSLSGTLGYVMS 202
+ ++ +L R+ +E+TVGAGLP+I +L ++ +GD + +I G SGTL Y+ +
Sbjct: 313 LSEYLRLRALQRQSYTHYFYEATVGAGLPIIGTLRGLLETGDKILQIEGIFSGTLSYIFN 492
Query: 203 EVEDGKPLSQVVRAAKSLGYTEPDPRDDLGGMD 235
+DG+ S+VV AK GYTEPDPRDDL G D
Sbjct: 493 NFKDGQVFSEVVAEAKEAGYTEPDPRDDLSGTD 591
>TC87813 similar to SP|P49299|CYSZ_CUCMA Citrate synthase glyoxysomal
precursor (EC 4.1.3.7) (GCS). [Pumpkin Winter squash],
partial (57%)
Length = 1235
Score = 29.3 bits (64), Expect = 2.6
Identities = 15/59 (25%), Positives = 27/59 (45%), Gaps = 4/59 (6%)
Frame = +3
Query: 195 GTLGYVMSEVEDGKPLSQVVRAAKSLGYTEPDPRDDLGGMDVARKALI----LARILGR 249
G L + + +D P+ +V A +L PD L G+D+ + + RI+G+
Sbjct: 690 GVLDLIQAMPQDAHPMGVLVNALSALSVFHPDANPALRGLDIYNSKQVRDKQIVRIIGK 866
>AJ388848 similar to GP|20805122|db protein kinase -like {Oryza sativa
(japonica cultivar-group)}, partial (44%)
Length = 557
Score = 27.3 bits (59), Expect = 9.8
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 194 SGTLGYVMSEVEDGKPLSQ 212
+GTLGY+ EV DGKP ++
Sbjct: 284 TGTLGYMAPEVLDGKPYNR 340
>TC87927 weakly similar to GP|12321870|gb|AAG50966.1 unknown protein;
53948-55359 {Arabidopsis thaliana}, partial (79%)
Length = 768
Score = 27.3 bits (59), Expect = 9.8
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = -3
Query: 16 SHLLQHIVSSRSLHSSQGLCLRVVGIGDS 44
SH+LQHI + +HSS +CL+ + S
Sbjct: 340 SHVLQHISILQFVHSSNKICLQQCAVQTS 254
>BG454627 similar to GP|15777895|gb SKIP5-like protein {Lycopersicon
esculentum}, partial (63%)
Length = 665
Score = 27.3 bits (59), Expect = 9.8
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +3
Query: 308 IEGPRCEVGIQELPKNSALGRLRGSDNVLEVYTRC 342
I P C +G E+P ++ L RG D+ E + C
Sbjct: 402 INKPLCLIGAGEIPDDTMLTCSRGLDSAFEFLSNC 506
>BE998520
Length = 525
Score = 27.3 bits (59), Expect = 9.8
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +2
Query: 115 CTASSDTIVVLKQVVDLGCCVVMANKKPLTSTM 147
C A + K+V+DLG C++ +KKP+ +
Sbjct: 38 CKAVDLNDEIKKKVLDLGACLLRGSKKPVAKEL 136
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.139 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,500,167
Number of Sequences: 36976
Number of extensions: 138545
Number of successful extensions: 568
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 564
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 566
length of query: 376
length of database: 9,014,727
effective HSP length: 98
effective length of query: 278
effective length of database: 5,391,079
effective search space: 1498719962
effective search space used: 1498719962
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146743.7