BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146723.12 + phase: 0
(172 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90461 similar to GP|21554792|gb|AAM63692.1 unknown {Arabidopsi... 355 7e-99
BQ123368 30 0.48
TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protei... 30 0.63
AL367192 weakly similar to GP|14326489|gb| AT5g10580/F12B17_70 {... 29 0.82
TC86788 similar to GP|21593695|gb|AAM65662.1 unknown {Arabidopsi... 29 1.1
CA989284 similar to PIR|S12404|A330 conglutin delta precursor - ... 28 1.4
CA858513 weakly similar to PIR|S61428|S614 embryonic abundant pr... 28 1.4
BQ124315 similar to GP|23510634|emb hypothetical protein {Plasmo... 28 1.4
TC85213 homologue to GP|13432086|gb|AAK20113.1 lipoxygenase {Gly... 28 1.4
CA858692 similar to PIR|S12404|A330 conglutin delta precursor - ... 28 1.4
CA989600 weakly similar to GP|14091584|gb| membrane protein Mlo7... 28 1.4
TC89089 homologue to SP|P43282|METM_LYCES S-adenosylmethionine s... 28 1.4
BQ124242 similar to PIR|S12404|A330 conglutin delta precursor - ... 28 1.4
BQ122325 28 1.4
TC85391 homologue to PIR|T06598|T06598 dnaK-type molecular chape... 28 1.8
TC80756 homologue to PIR|S53498|S53498 dnaK-type molecular chape... 28 1.8
TC85392 homologue to PIR|JC4786|JC4786 dnaK-type molecular chape... 28 2.4
TC85390 homologue to SP|P27322|HS72_LYCES Heat shock cognate 70 ... 28 2.4
TC93111 28 2.4
BI271089 similar to GP|9294325|dbj| gene_id:K24M9.13~unknown pro... 28 2.4
>TC90461 similar to GP|21554792|gb|AAM63692.1 unknown {Arabidopsis
thaliana}, partial (60%)
Length = 799
Score = 355 bits (910), Expect = 7e-99
Identities = 172/172 (100%), Positives = 172/172 (100%)
Frame = +2
Query: 1 MASSTWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSIKRSITRA 60
MASSTWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSIKRSITRA
Sbjct: 98 MASSTWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSIKRSITRA 277
Query: 61 AEYKFPDPIPEFADSETEKFQNHLLNKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGP 120
AEYKFPDPIPEFADSETEKFQNHLLNKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGP
Sbjct: 278 AEYKFPDPIPEFADSETEKFQNHLLNKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGP 457
Query: 121 GTLLVDPFIDMADIVNERGLPGGPQAARAAINWAQAHVDKDWNEWTGGNSNE 172
GTLLVDPFIDMADIVNERGLPGGPQAARAAINWAQAHVDKDWNEWTGGNSNE
Sbjct: 458 GTLLVDPFIDMADIVNERGLPGGPQAARAAINWAQAHVDKDWNEWTGGNSNE 613
>BQ123368
Length = 180
Score = 30.0 bits (66), Expect = 0.48
Identities = 17/44 (38%), Positives = 27/44 (60%)
Frame = -1
Query: 4 STWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVST 47
S+W L+P ++ +SSSS LC T++C+ RF S R +S+
Sbjct: 141 SSWWLIPLILSTSSSSF*ILCI-MCTSQCL-RFKLWSFLRHLSS 16
>TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protein 4 precursor
(BiP 4) (78 kDa glucose-regulated protein homolog 4) (GRP
78-4)., partial (94%)
Length = 2265
Score = 29.6 bits (65), Expect = 0.63
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +2
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+ +K+ FEE ++EV VC I + G PG
Sbjct: 1898 NQTVEKEEFEEKLKEVEAVCNPIITAVYQRSGGAPG 2005
>AL367192 weakly similar to GP|14326489|gb| AT5g10580/F12B17_70 {Arabidopsis
thaliana}, partial (24%)
Length = 246
Score = 29.3 bits (64), Expect = 0.82
Identities = 13/17 (76%), Positives = 14/17 (81%)
Frame = -3
Query: 10 PFLIPSSSSSIPSLCFS 26
P L P+S SSIPSLCFS
Sbjct: 100 PILHPNSISSIPSLCFS 50
>TC86788 similar to GP|21593695|gb|AAM65662.1 unknown {Arabidopsis
thaliana}, partial (78%)
Length = 1705
Score = 28.9 bits (63), Expect = 1.1
Identities = 15/32 (46%), Positives = 18/32 (55%), Gaps = 1/32 (3%)
Frame = -2
Query: 21 PSLCFSQTTTKCIQRFSFP-SINRKVSTLPFS 51
P+LCFS +TT C FP S R S PF+
Sbjct: 540 PALCFSPSTTNCSVA*PFPCSAKRSFSIRPFT 445
>CA989284 similar to PIR|S12404|A330 conglutin delta precursor -
narrow-leaved blue lupine, partial (14%)
Length = 232
Score = 28.5 bits (62), Expect = 1.4
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = -3
Query: 4 STWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVST 47
S+W L+P ++ +SSSS LC +++C+ RF S R +S+
Sbjct: 134 SSWWLIPLILSTSSSSF*ILCI-MCSSQCL-RFKLWSFLRHLSS 9
>CA858513 weakly similar to PIR|S61428|S614 embryonic abundant protein group
3 precursor (clone PM10) - soybean, partial (19%)
Length = 869
Score = 28.5 bits (62), Expect = 1.4
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = -3
Query: 4 STWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVST 47
S+W L+P ++ +SSSS LC +++C+ RF S R +S+
Sbjct: 222 SSWWLIPLILSTSSSSF*ILCI-MCSSQCL-RFKLWSFLRHLSS 97
>BQ124315 similar to GP|23510634|emb hypothetical protein {Plasmodium
falciparum 3D7}, partial (1%)
Length = 681
Score = 28.5 bits (62), Expect = 1.4
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = -2
Query: 4 STWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVST 47
S+W L+P ++ +SSSS LC +++C+ RF S R +S+
Sbjct: 203 SSWWLIPLILSTSSSSF*ILCI-MCSSQCL-RFKLWSFLRHLSS 78
>TC85213 homologue to GP|13432086|gb|AAK20113.1 lipoxygenase {Glycine max},
partial (4%)
Length = 747
Score = 28.5 bits (62), Expect = 1.4
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = +1
Query: 4 STWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVST 47
S+W L+P ++ +SSSS LC +++C+ RF S R +S+
Sbjct: 421 SSWWLIPLILSTSSSSF*ILCI-MCSSQCL-RFKLWSFLRHLSS 546
>CA858692 similar to PIR|S12404|A330 conglutin delta precursor -
narrow-leaved blue lupine, partial (14%)
Length = 215
Score = 28.5 bits (62), Expect = 1.4
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = -1
Query: 4 STWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVST 47
S+W L+P ++ +SSSS LC +++C+ RF S R +S+
Sbjct: 197 SSWWLIPLILSTSSSSF*ILCI-MCSSQCL-RFKLWSFLRHLSS 72
>CA989600 weakly similar to GP|14091584|gb| membrane protein Mlo7
{Arabidopsis thaliana}, partial (5%)
Length = 402
Score = 28.5 bits (62), Expect = 1.4
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = -2
Query: 4 STWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVST 47
S+W L+P ++ +SSSS LC +++C+ RF S R +S+
Sbjct: 203 SSWWLIPLILSTSSSSF*ILCI-MCSSQCL-RFKLWSFLRHLSS 78
>TC89089 homologue to SP|P43282|METM_LYCES S-adenosylmethionine synthetase
3 (EC 2.5.1.6) (Methionine adenosyltransferase 3),
partial (72%)
Length = 944
Score = 28.5 bits (62), Expect = 1.4
Identities = 18/41 (43%), Positives = 23/41 (55%), Gaps = 1/41 (2%)
Frame = +2
Query: 2 ASSTWCLVP-FLIPSSSSSIPSLCFSQTTTKCIQRFSFPSI 41
+SST+ L P LI SSS +L T T C+ RF PS+
Sbjct: 53 SSSTFSLQPPHLIWKLSSSPQNLSTKVTPTSCVIRFQMPSL 175
>BQ124242 similar to PIR|S12404|A330 conglutin delta precursor -
narrow-leaved blue lupine, partial (14%)
Length = 391
Score = 28.5 bits (62), Expect = 1.4
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = -3
Query: 4 STWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVST 47
S+W L+P ++ +SSSS LC +++C+ RF S R +S+
Sbjct: 197 SSWWLIPLILSTSSSSF*ILCI-MCSSQCL-RFKLWSFLRHLSS 72
>BQ122325
Length = 346
Score = 28.5 bits (62), Expect = 1.4
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = -3
Query: 4 STWCLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVST 47
S+W L+P ++ +SSSS LC +++C+ RF S R +S+
Sbjct: 218 SSWWLIPLILSTSSSSF*ILCI-MCSSQCL-RFKLWSFLRHLSS 93
>TC85391 homologue to PIR|T06598|T06598 dnaK-type molecular chaperone BiP-A
- soybean, partial (52%)
Length = 1231
Score = 28.1 bits (61), Expect = 1.8
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+ +K+ +EE ++EV VC I + G PG
Sbjct: 889 NQSVEKEEYEEKLKEVEAVCNPIITAVYQRSGGAPG 996
>TC80756 homologue to PIR|S53498|S53498 dnaK-type molecular chaperone
HSP71.2 - garden pea, partial (53%)
Length = 1189
Score = 28.1 bits (61), Expect = 1.8
Identities = 15/58 (25%), Positives = 26/58 (43%)
Frame = +3
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPGTLLVDPFIDMADIVNERGLPGG 143
N++++ D FE+ +E+ G+C I + GG D+ G+PGG
Sbjct: 864 NQMAEVDEFEDKQKELEGICNPIIAKMYQGGAGG-------------DVPMGDGMPGG 998
>TC85392 homologue to PIR|JC4786|JC4786 dnaK-type molecular chaperone hsc70-3
- tomato, complete
Length = 2295
Score = 27.7 bits (60), Expect = 2.4
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +1
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+L + D FE+ ++E+ G+C I + G G
Sbjct: 1897 NQLGEADEFEDKMKELEGICNPIIARMYQGAGGDAG 2004
>TC85390 homologue to SP|P27322|HS72_LYCES Heat shock cognate 70 kDa protein
2. [Tomato] {Lycopersicon esculentum}, complete
Length = 2278
Score = 27.7 bits (60), Expect = 2.4
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +1
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+L + D FE+ ++E+ G+C I + G G
Sbjct: 1903 NQLGEADEFEDKMKELEGICNPIIARMYQGAGGDAG 2010
>TC93111
Length = 793
Score = 27.7 bits (60), Expect = 2.4
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = +3
Query: 7 CLVPFLIPSSSSSIPSLCFSQTTTKCIQRFSFPSINRKVSTLPFSSIK 54
C + P P + +S + IQ+FSF S+++K PF K
Sbjct: 177 CFSLSVFPFLKQLNPLVTYSYLMNQHIQKFSFQSVHQKFIVWPFKHFK 320
>BI271089 similar to GP|9294325|dbj| gene_id:K24M9.13~unknown protein
{Arabidopsis thaliana}, partial (2%)
Length = 481
Score = 27.7 bits (60), Expect = 2.4
Identities = 20/63 (31%), Positives = 32/63 (50%), Gaps = 8/63 (12%)
Frame = -3
Query: 4 STWCLVPFLIPSSSSSIPS--------LCFSQTTTKCIQRFSFPSINRKVSTLPFSSIKR 55
STW P +IP SS+ P+ F+ +T ++F+ SIN V +P + +KR
Sbjct: 296 STWISCPSIIP*SSNRAPTCLRIVRKPTMFTISTISTSKKFA-RSINSAVRHMPATVLKR 120
Query: 56 SIT 58
I+
Sbjct: 119 IIS 111
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.132 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,323,420
Number of Sequences: 36976
Number of extensions: 94518
Number of successful extensions: 704
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 700
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 704
length of query: 172
length of database: 9,014,727
effective HSP length: 89
effective length of query: 83
effective length of database: 5,723,863
effective search space: 475080629
effective search space used: 475080629
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146723.12