BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146722.7 + phase: 1 /pseudo/partial
(293 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW776255 weakly similar to PIR|T07672|T07 cyclin a2-type mitosi... 171 3e-43
AW299045 weakly similar to GP|18175785|gb putative cyclin {Arabi... 124 4e-29
AW775066 similar to PIR|T07669|T07 cyclin a1-type mitosis-speci... 82 3e-16
AL377325 homologue to GP|3608181|dbj cyclin B {Pisum sativum}, p... 77 8e-15
AL383194 similar to GP|6031209|gb| cyclin {Pisum sativum}, parti... 76 1e-14
TC84410 similar to GP|15419009|gb|AAK81695.1 cyclin A2 {Medicago... 65 2e-11
AW696887 similar to PIR|T07669|T07 cyclin a1-type mitosis-speci... 64 9e-11
TC92772 similar to GP|19347837|gb|AAL86330.1 putative prolyl end... 55 3e-08
BG588004 similar to GP|7242793|emb| cyclin A3.1 {Pisum sativum},... 54 5e-08
TC79129 similar to PIR|A96725|A96725 hypothetical protein F20P5.... 33 0.100
TC90916 similar to PIR|T09961|T09961 cyclin D-like protein - red... 30 0.84
BQ141426 30 1.4
CA859484 29 1.9
TC83223 similar to GP|8777436|dbj|BAA97026.1 gb|AAC78547.1~gene_... 28 5.5
TC82421 alpha (1,3/1,4) fucosyltransferase [Medicago truncatula] 27 7.1
TC85396 homologue to GP|6911551|emb|CAB72129.1 heat shock protei... 27 9.3
>AW776255 weakly similar to PIR|T07672|T07 cyclin a2-type mitosis-specific -
soybean, partial (22%)
Length = 704
Score = 171 bits (433), Expect = 3e-43
Identities = 83/83 (100%), Positives = 83/83 (100%)
Frame = +1
Query: 1 INLICEKLSASVGLGIVDIDSKLRDSPIWTSYAPDIYTNIHVRECERRPLANYMETLQQD 60
INLICEKLSASVGLGIVDIDSKLRDSPIWTSYAPDIYTNIHVRECERRPLANYMETLQQD
Sbjct: 436 INLICEKLSASVGLGIVDIDSKLRDSPIWTSYAPDIYTNIHVRECERRPLANYMETLQQD 615
Query: 61 ITPGMRGILVDWLVEVADEFKLV 83
ITPGMRGILVDWLVEVADEFKLV
Sbjct: 616 ITPGMRGILVDWLVEVADEFKLV 684
>AW299045 weakly similar to GP|18175785|gb putative cyclin {Arabidopsis
thaliana}, partial (24%)
Length = 646
Score = 124 bits (311), Expect = 4e-29
Identities = 59/91 (64%), Positives = 71/91 (77%)
Frame = -1
Query: 186 DLEFLANYLAELALVEYSFLQFQPSKIAASSVLLARWTLNQSEHPWNPTLEHYTNYKASE 245
+LE+LAN+LAEL L+ Y FL F PS IAAS+V LARWTL+QS HPWNPTLEHY +YKAS+
Sbjct: 628 ELEYLANFLAELTLMSYGFLNFFPSMIAASAVFLARWTLDQSRHPWNPTLEHYASYKASD 449
Query: 246 LKTTVLELIDLQLNTKRCRLNAVREKYQHQK 276
LK TVL L +LQLN+ C A+R KY+ K
Sbjct: 448 LKATVLALQNLQLNSDDCPYPAIRTKYRQSK 356
>AW775066 similar to PIR|T07669|T07 cyclin a1-type mitosis-specific -
soybean, partial (31%)
Length = 696
Score = 81.6 bits (200), Expect = 3e-16
Identities = 42/94 (44%), Positives = 60/94 (63%)
Frame = +2
Query: 187 LEFLANYLAELALVEYSFLQFQPSKIAASSVLLARWTLNQSEHPWNPTLEHYTNYKASEL 246
+EFL NYLAEL+L++Y ++F PS +AAS + LAR+ + HP +L YK++EL
Sbjct: 11 MEFLCNYLAELSLIDYECIRFLPSMVAASVIFLARFIICPGVHPLTSSLSECLFYKSAEL 190
Query: 247 KTTVLELIDLQLNTKRCRLNAVREKYQHQKVRNL 280
+ VL L DL L + L AVREKY+ K +N+
Sbjct: 191 EECVLILHDLYLVRRAASLKAVREKYKQHKFKNV 292
>AL377325 homologue to GP|3608181|dbj cyclin B {Pisum sativum}, partial (45%)
Length = 329
Score = 77.0 bits (188), Expect = 8e-15
Identities = 40/91 (43%), Positives = 59/91 (63%), Gaps = 1/91 (1%)
Frame = +2
Query: 121 YEEICAPGVEDFCVITDNTYSRQEVLKMEKEVLNLLHFQLAVPTIKTFLRRFIQVAQTSS 180
YEEI P V DF ++D YS +++L MEK +L L + L VPT FL RFI+ A S+
Sbjct: 56 YEEIWPPEVNDFVCLSDRAYSHEQILIMEKTILGKLEWTLTVPTPFVFLVRFIKAASVSA 235
Query: 181 KVA-QADLEFLANYLAELALVEYSFLQFQPS 210
+ Q DLE +A++L+EL ++ Y+ L++ PS
Sbjct: 236 VPSDQGDLEMMAHFLSELGMMHYATLRYCPS 328
>AL383194 similar to GP|6031209|gb| cyclin {Pisum sativum}, partial (55%)
Length = 435
Score = 76.3 bits (186), Expect = 1e-14
Identities = 46/136 (33%), Positives = 73/136 (52%)
Frame = +2
Query: 142 RQEVLKMEKEVLNLLHFQLAVPTIKTFLRRFIQVAQTSSKVAQADLEFLANYLAELALVE 201
R ++L MEK +L L F+L PT FL RF++ AQ+ K LE +A +L +L LVE
Sbjct: 2 RDQMLGMEKLILRKLKFRLNAPTPYVFLIRFLKAAQSDMK-----LEHMAFFLIDLCLVE 166
Query: 202 YSFLQFQPSKIAASSVLLARWTLNQSEHPWNPTLEHYTNYKASELKTTVLELIDLQLNTK 261
Y L F+PS + AS + +AR TL + W P L+ + Y+ S++ ++
Sbjct: 167 YEALAFKPSLLCASXLYVARCTLXITP-SWTPLLQKHARYEVSQIXDCADMILKFHKAAG 343
Query: 262 RCRLNAVREKYQHQKV 277
+ +L EKY +++
Sbjct: 344 KGKLTVAYEKYSRKEL 391
>TC84410 similar to GP|15419009|gb|AAK81695.1 cyclin A2 {Medicago sativa},
partial (23%)
Length = 771
Score = 65.5 bits (158), Expect = 2e-11
Identities = 32/67 (47%), Positives = 43/67 (63%)
Frame = +1
Query: 16 IVDIDSKLRDSPIWTSYAPDIYTNIHVRECERRPLANYMETLQQDITPGMRGILVDWLVE 75
I +ID+ + D + + YA DIY N+ V E N+MET+Q+DIT MR IL+DWLVE
Sbjct: 547 IPNIDADIEDPQLCSFYAADIYDNLRVAELSXXXHPNFMETVQRDITXXMRAILIDWLVE 726
Query: 76 VADEFKL 82
V+ F L
Sbjct: 727 VSXXFNL 747
>AW696887 similar to PIR|T07669|T07 cyclin a1-type mitosis-specific -
soybean, partial (33%)
Length = 648
Score = 63.5 bits (153), Expect = 9e-11
Identities = 44/120 (36%), Positives = 65/120 (53%), Gaps = 5/120 (4%)
Frame = +3
Query: 19 IDSKLRDSPIWTSYAPDIYTNIHVRECE--RRPLANYMETLQQDITPGMRGILVDWLVEV 76
I++K + I Y DI + E + RRP+ Y+E +Q+ I+ MRG LVDWLVEV
Sbjct: 294 INAKRDNQHIIQPYVSDISDYLRTMEMQKKRRPMVGYLENVQRGISSNMRGTLVDWLVEV 473
Query: 77 ADEFKLVPRHTLPCCESH*SIPFPTFDYQTKAAVAWYYLHADFI---YEEICAPGVEDFC 133
ADE+KL+P TL S+ F + + +++ + + + I YEEI P DFC
Sbjct: 474 ADEYKLLP-ETLHLSVSYID-RFLSIEPVSRSKLQLLGVSSMLIASKYEEITPPKAVDFC 647
>TC92772 similar to GP|19347837|gb|AAL86330.1 putative prolyl endopeptidase
{Arabidopsis thaliana}, partial (14%)
Length = 531
Score = 55.1 bits (131), Expect = 3e-08
Identities = 28/52 (53%), Positives = 34/52 (64%)
Frame = +3
Query: 121 YEEICAPGVEDFCVITDNTYSRQEVLKMEKEVLNLLHFQLAVPTIKTFLRRF 172
YEEI AP V DF I+DN Y R++VL MEK +L L + L VPT FL R+
Sbjct: 375 YEEIWAPEVNDFVCISDNAYVREQVLVMEKTILRNLEWYLTVPTPYVFLVRY 530
>BG588004 similar to GP|7242793|emb| cyclin A3.1 {Pisum sativum}, partial
(22%)
Length = 713
Score = 54.3 bits (129), Expect = 5e-08
Identities = 28/76 (36%), Positives = 44/76 (57%), Gaps = 2/76 (2%)
Frame = +2
Query: 9 SASVGLGIVDIDSKLRDSPIWTSYAPDIYTNIHVRECE--RRPLANYMETLQQDITPGMR 66
S S G + + K D + +Y DIY + E + +RPL +Y++ +Q+D+ MR
Sbjct: 470 SNSDGTPVPEKLEKFEDPQLCETYVSDIYDYLRNMEVDSSKRPLCDYIQKVQRDVNASMR 649
Query: 67 GILVDWLVEVADEFKL 82
G+LVDWL EVA++ L
Sbjct: 650 GVLVDWLGEVAEDTSL 697
>TC79129 similar to PIR|A96725|A96725 hypothetical protein F20P5.7
[imported] - Arabidopsis thaliana, partial (64%)
Length = 1328
Score = 33.5 bits (75), Expect = 0.100
Identities = 32/117 (27%), Positives = 54/117 (45%), Gaps = 9/117 (7%)
Frame = +2
Query: 122 EEICAPGVEDFCVI-TDNTYSRQEVLKMEKEVLNLLHFQLAVPTIKTFLRRFIQVAQTSS 180
EE P + DF + + + +L+ME VL +L ++L T +FL F ++
Sbjct: 542 EEPLVPSLLDFQIEGAKYIFQPRTILRMELLVLTILDWRLRSITPLSFLSFFACKLDSTG 721
Query: 181 KVAQADLEFLANYLAELALV---EYSFLQFQPSKIAASSVLLA-----RWTLNQSEH 229
F+ + E+ L + SFL ++PS IAA+++L A W+ EH
Sbjct: 722 TFTH----FIISRATEIILSNIQDASFLTYRPSCIAAAAILSAANEIPNWSFVNPEH 880
>TC90916 similar to PIR|T09961|T09961 cyclin D-like protein - red goosefoot,
partial (31%)
Length = 886
Score = 30.4 bits (67), Expect = 0.84
Identities = 18/76 (23%), Positives = 38/76 (49%)
Frame = +3
Query: 140 YSRQEVLKMEKEVLNLLHFQLAVPTIKTFLRRFIQVAQTSSKVAQADLEFLANYLAELAL 199
+ + + +ME VL+ L +++ T +F+ F+ + K + + + L +
Sbjct: 138 FEAKTIQRMELLVLSTLKWRMQAITPFSFIECFLSKIKDDDKSSLSSSISRSTQLISSTI 317
Query: 200 VEYSFLQFQPSKIAAS 215
FL+F+PS+IAA+
Sbjct: 318 KGLDFLEFKPSEIAAA 365
>BQ141426
Length = 471
Score = 29.6 bits (65), Expect = 1.4
Identities = 28/126 (22%), Positives = 54/126 (42%), Gaps = 5/126 (3%)
Frame = +3
Query: 52 NYMETLQQDITPGMRGILVDWLVEVADEFKLVPRH--TLPCCESH*SIPFPTFDYQTKAA 109
NY+E +Q + L+DW +E +E + L + H + F +F +K
Sbjct: 12 NYLELIQPLVIMDELAFLMDWDLEAINEVPTTNPYFSHLFSEQDHDELLFGSFPEFSKTT 191
Query: 110 VAWYYLHADFIYEEICAPG---VEDFCVITDNTYSRQEVLKMEKEVLNLLHFQLAVPTIK 166
+H +EE+C P + ++T + + +E + KE+ H L +P +
Sbjct: 192 NV--LIHE---FEELCKPNFYPLSSQTILTSSLATPKEP-EQVKEIKAASHQDLQIPAVS 353
Query: 167 TFLRRF 172
F +R+
Sbjct: 354 KFKKRY 371
>CA859484
Length = 221
Score = 29.3 bits (64), Expect = 1.9
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = +3
Query: 225 NQSEHPWNPTLEHYTNYKASELKTTVLEL 253
+++ PWN T+E Y Y A +K +LEL
Sbjct: 6 DKTVQPWN*TIEEYKKYLAKGVKV*ILEL 92
>TC83223 similar to GP|8777436|dbj|BAA97026.1
gb|AAC78547.1~gene_id:MHM17.7~similar to unknown protein
{Arabidopsis thaliana}, partial (13%)
Length = 514
Score = 27.7 bits (60), Expect = 5.5
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = -1
Query: 206 QFQPSKIAASSVLLARWTL 224
QFQPSKI+ S +LL+ W L
Sbjct: 349 QFQPSKISMSPLLLSVWKL 293
>TC82421 alpha (1,3/1,4) fucosyltransferase [Medicago truncatula]
Length = 1044
Score = 27.3 bits (59), Expect = 7.1
Identities = 12/31 (38%), Positives = 19/31 (60%)
Frame = -3
Query: 239 TNYKASELKTTVLELIDLQLNTKRCRLNAVR 269
+N KAS L L+L+ QLN +R R+ ++
Sbjct: 109 SNIKASGLSARTLDLVHKQLNPQRSRIQIIQ 17
>TC85396 homologue to GP|6911551|emb|CAB72129.1 heat shock protein 70 {Cucumis
sativus}, complete
Length = 2320
Score = 26.9 bits (58), Expect = 9.3
Identities = 12/31 (38%), Positives = 22/31 (70%)
Frame = +3
Query: 141 SRQEVLKMEKEVLNLLHFQLAVPTIKTFLRR 171
++ E++K++K ++ LLH Q+AV + LRR
Sbjct: 1956 TKVELVKVQKLMMMLLHLQVAVAVLVPRLRR 2048
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.137 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,576,299
Number of Sequences: 36976
Number of extensions: 133853
Number of successful extensions: 813
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 806
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 810
length of query: 293
length of database: 9,014,727
effective HSP length: 95
effective length of query: 198
effective length of database: 5,502,007
effective search space: 1089397386
effective search space used: 1089397386
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146722.7