BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146719.10 + phase: 0 /pseudo
(112 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86853 similar to GP|20198043|gb|AAD21708.2 26S proteasome regu... 153 8e-39
TC78010 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis ... 27 1.5
TC84467 weakly similar to GP|10178088|dbj|BAB11507. contains sim... 26 2.0
TC85652 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis ... 26 2.6
TC85651 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis ... 26 2.6
TC89200 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis ... 25 3.4
BF641496 weakly similar to PIR|T01271|T012 serine/threonine-spec... 25 3.4
AW573674 similar to PIR|S44261|S442 SRG1 protein - Arabidopsis t... 25 4.5
TC93020 similar to GP|6635840|gb|AAF20003.1| 1-acyl-sn-glycerol-... 25 4.5
AL375121 25 5.9
TC86720 similar to PIR|T47941|T47941 hypothetical protein F2A19.... 25 5.9
TC87301 similar to SP|P46637|ARG1_ARATH Arginase (EC 3.5.3.1). [... 24 7.7
BE940916 24 7.7
>TC86853 similar to GP|20198043|gb|AAD21708.2 26S proteasome regulatory
subunit S2 (RPN1) {Arabidopsis thaliana}, partial (55%)
Length = 1738
Score = 153 bits (387), Expect = 8e-39
Identities = 81/110 (73%), Positives = 90/110 (81%)
Frame = +3
Query: 2 LELPSV**GKYHYVLYFLVLAMQLTLGYYILLQKPTMLLTVGENLKPLLVPLRVGQAVDV 61
L++ ++ GKYHYVLYFLVLAMQ P MLLTV E+LKPL VP+RVGQAVDV
Sbjct: 1206 LDMKAIVLGKYHYVLYFLVLAMQ-----------PRMLLTVDEDLKPLSVPVRVGQAVDV 1352
Query: 62 VGQAGRPKKITGFQTHSTSVLLAAGDRAELATEKYIPISPVLEAFVILKE 111
VGQAGRPK ITGFQTHST VLLAAGDRAELATEKY+PISP+LE FVILK+
Sbjct: 1353 VGQAGRPKTITGFQTHSTPVLLAAGDRAELATEKYLPISPILEGFVILKD 1502
>TC78010 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis thaliana,
partial (47%)
Length = 1321
Score = 26.6 bits (57), Expect = 1.5
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 7/49 (14%)
Frame = +3
Query: 67 RPKKITGFQTHST----SVLLAAGDRAELATEK---YIPISPVLEAFVI 108
+P K+ G HS ++LL + L +K +IPI P+ AFVI
Sbjct: 720 QPDKVIGLNPHSDGTALTILLQLNEIEGLQIKKDGMWIPIKPLTNAFVI 866
>TC84467 weakly similar to GP|10178088|dbj|BAB11507. contains similarity to
calmodulin-binding protein~gene_id:K19B1.18 {Arabidopsis
thaliana}, partial (18%)
Length = 880
Score = 26.2 bits (56), Expect = 2.0
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1
Query: 14 YVLYFLVLAMQLTLGYYILL 33
++ YFL LA Q T YYIL+
Sbjct: 175 FISYFLFLASQYTFYYYILV 234
>TC85652 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis thaliana,
partial (50%)
Length = 1489
Score = 25.8 bits (55), Expect = 2.6
Identities = 16/49 (32%), Positives = 25/49 (50%), Gaps = 7/49 (14%)
Frame = +1
Query: 67 RPKKITGFQTHST----SVLLAAGDRAELATEK---YIPISPVLEAFVI 108
+P+ + G HS ++LL A + L K +IP+ P+ AFVI
Sbjct: 898 QPENVIGLNPHSDVGALTILLQANEIEGLQIRKDGQWIPVQPLPNAFVI 1044
>TC85651 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis thaliana,
partial (39%)
Length = 1269
Score = 25.8 bits (55), Expect = 2.6
Identities = 15/49 (30%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +3
Query: 67 RPKKITGFQTHSTS----VLLAAGDRAELATEK---YIPISPVLEAFVI 108
+P ++ G HS + +LL + L +K +IPI P+ +AFV+
Sbjct: 657 QPDQVIGLNPHSDAGTLTILLQVNEMEGLQIKKDGIWIPIRPLSDAFVV 803
>TC89200 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis thaliana,
partial (47%)
Length = 1323
Score = 25.4 bits (54), Expect = 3.4
Identities = 16/49 (32%), Positives = 25/49 (50%), Gaps = 7/49 (14%)
Frame = +3
Query: 67 RPKKITGFQTHST----SVLLAAGDRAELATEK---YIPISPVLEAFVI 108
+P+++ G HS ++LL + L K +IPI P+ AFVI
Sbjct: 783 QPEQVIGLNPHSDVGALTILLQLNEIEGLQVRKDGMWIPIKPLSNAFVI 929
>BF641496 weakly similar to PIR|T01271|T012 serine/threonine-specific protein
kinase (EC 2.7.1.-) F27F23.3 - Arabidopsis thaliana,
partial (5%)
Length = 631
Score = 25.4 bits (54), Expect = 3.4
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = -1
Query: 17 YFLVLAMQLTLGYYILLQKPTMLLTVGENLKPLL 50
YF L + + +Y+LL+ +MLL V ++ L+
Sbjct: 424 YFFPLLVSMCSSFYLLLESISMLLIVSQSKDSLV 323
>AW573674 similar to PIR|S44261|S442 SRG1 protein - Arabidopsis thaliana,
partial (27%)
Length = 610
Score = 25.0 bits (53), Expect = 4.5
Identities = 15/49 (30%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +2
Query: 67 RPKKITGFQTHST----SVLLAAGDRAELATEK---YIPISPVLEAFVI 108
+P+ + G HS ++LL A + L +K +IP+ P+ AF+I
Sbjct: 332 QPELVMGINPHSDGSGLTILLQANEVEGLQIKKDELWIPVKPLPNAFII 478
>TC93020 similar to GP|6635840|gb|AAF20003.1| 1-acyl-sn-glycerol-3-phosphate
acyltransferase {Prunus dulcis}, partial (10%)
Length = 784
Score = 25.0 bits (53), Expect = 4.5
Identities = 12/45 (26%), Positives = 23/45 (50%)
Frame = +2
Query: 18 FLVLAMQLTLGYYILLQKPTMLLTVGENLKPLLVPLRVGQAVDVV 62
FL++ LGY +KP +T E++ L+PL + + ++
Sbjct: 584 FLLMGSFFGLGYLHKPRKPINFVTFDESVSFSLIPLNIYYLISLI 718
>AL375121
Length = 379
Score = 24.6 bits (52), Expect = 5.9
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +2
Query: 16 LYFLVLAMQLTLGYYI-LLQKPTMLLTVGENLK 47
LYFL L + LTL Y++ LL + ++ T + K
Sbjct: 176 LYFLTLTLTLTLFYWVNLLSEVHVIFTTYNHFK 274
>TC86720 similar to PIR|T47941|T47941 hypothetical protein F2A19.90 -
Arabidopsis thaliana, partial (85%)
Length = 1719
Score = 24.6 bits (52), Expect = 5.9
Identities = 15/46 (32%), Positives = 21/46 (45%), Gaps = 9/46 (19%)
Frame = -2
Query: 13 HYVLYFLVLAMQLTLGYYILLQ---------KPTMLLTVGENLKPL 49
HYVLY L Q+ L ++L P +LL + E+ PL
Sbjct: 272 HYVLYNLYHCTQMKLSQFLLFYFQLFLDTPVSPQILLKIFESFVPL 135
>TC87301 similar to SP|P46637|ARG1_ARATH Arginase (EC 3.5.3.1). [Mouse-ear
cress] {Arabidopsis thaliana}, partial (91%)
Length = 1310
Score = 24.3 bits (51), Expect = 7.7
Identities = 11/31 (35%), Positives = 19/31 (60%)
Frame = +2
Query: 53 LRVGQAVDVVGQAGRPKKITGFQTHSTSVLL 83
L +G+ VV Q +PKK+ ++TH ++L
Sbjct: 377 LALGRPFGVVVQTRQPKKVRIYRTHEC*LML 469
>BE940916
Length = 632
Score = 24.3 bits (51), Expect = 7.7
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = +2
Query: 11 KYHYVLYFLVLAMQLTLGYYIL 32
+YH +L + L L LG YIL
Sbjct: 26 QYHLILILITLVPNLKLGSYIL 91
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.333 0.149 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,003,877
Number of Sequences: 36976
Number of extensions: 32976
Number of successful extensions: 210
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of query: 112
length of database: 9,014,727
effective HSP length: 88
effective length of query: 24
effective length of database: 5,760,839
effective search space: 138260136
effective search space used: 138260136
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 50 (23.9 bits)
Medicago: description of AC146719.10