BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146683.3 + phase: 0 /pseudo
(122 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG587463 similar to GP|15810473|gb| putative protein transport p... 49 9e-10
TC86340 similar to GP|9294523|dbj|BAB02785.1 protein transport p... 42 4e-05
TC88272 weakly similar to PIR|E84603|E84603 probable protein tra... 32 0.078
TC88956 similar to PIR|C84514|C84514 hypothetical protein At2g14... 28 1.1
BQ144458 GP|21740724|em OSJNBa0086B14.17 {Oryza sativa}, partial... 27 2.5
TC77439 similar to PIR|H84788|H84788 probable peroxidase ATP2a [... 26 4.3
TC76392 similar to GP|14030701|gb|AAK53025.1 At2g28370/T1B3.11 {... 26 4.3
TC88944 type IIB calcium ATPase [Medicago truncatula] 26 4.3
TC76379 similar to GP|14030701|gb|AAK53025.1 At2g28370/T1B3.11 {... 26 4.3
TC90070 similar to PIR|A90125|A90125 60S ribosomal protein L18A ... 26 4.3
TC81479 similar to GP|15215660|gb|AAK91375.1 At1g15870/F7H2_19 {... 26 4.3
AL384478 weakly similar to PIR|T39353|T393 probable splicing fac... 25 7.3
TC92661 similar to GP|22093674|dbj|BAC06968. kinase-like protein... 25 7.3
AL387173 25 7.3
TC86934 similar to PIR|T04630|T04630 synaptobrevin homolog F10N7... 25 7.3
TC76421 homologue to PIR|T02995|T02995 unspecific monooxygenase ... 25 7.3
CB891727 similar to GP|10716949|gb| SKP1 interacting partner 2 {... 25 7.3
TC76312 GP|8118527|gb|AAF73016.1| ubiquitin conjugating protein ... 25 7.3
AW692464 25 7.3
BG450380 similar to PIR|T10971|T10 Pv42 protein - kidney bean, p... 25 9.6
>BG587463 similar to GP|15810473|gb| putative protein transport protein SEC23
{Arabidopsis thaliana}, partial (3%)
Length = 401
Score = 48.5 bits (114), Expect(2) = 9e-10
Identities = 26/35 (74%), Positives = 28/35 (79%), Gaps = 3/35 (8%)
Frame = +3
Query: 32 ITVAIEDIHSTYNFKSRN---RSTGVAFSAALGLL 63
IT AIEDIHS+YNF+S N RSTG A SAALGLL
Sbjct: 297 ITAAIEDIHSSYNFRSGNRPPRSTGAAISAALGLL 401
Score = 29.3 bits (64), Expect(2) = 9e-10
Identities = 11/12 (91%), Positives = 12/12 (99%)
Frame = +2
Query: 22 WTNTACSKAGIT 33
WTNTACSKAGI+
Sbjct: 233 WTNTACSKAGIS 268
>TC86340 similar to GP|9294523|dbj|BAB02785.1 protein transport protein
Sec23 {Arabidopsis thaliana}, partial (96%)
Length = 2988
Score = 42.4 bits (98), Expect = 4e-05
Identities = 21/55 (38%), Positives = 29/55 (52%), Gaps = 5/55 (9%)
Frame = +1
Query: 50 RSTGVAFSAALGLLECCFVNTGSRIMVF-----ASGPGLVLDSDFWQSMRTHNDI 99
R TGVA S A GLL C TG+RI+ GPG ++ + +R+H D+
Sbjct: 919 RCTGVALSVAAGLLSACNPGTGARIIALVGGPCTEGPGTIVSKELSDQVRSHKDL 1083
>TC88272 weakly similar to PIR|E84603|E84603 probable protein transport
protein SEC23 [imported] - Arabidopsis thaliana, partial
(39%)
Length = 1061
Score = 31.6 bits (70), Expect = 0.078
Identities = 16/55 (29%), Positives = 25/55 (45%), Gaps = 5/55 (9%)
Frame = +3
Query: 50 RSTGVAFSAALGLLECCFVNTGSRIMVFASGPGL-----VLDSDFWQSMRTHNDI 99
+ T A S A LL C + +RIM F GP ++ + +R+H D+
Sbjct: 846 KCTSTALSIAASLLGACVPGSAARIMAFIGGPATEGPAPIVSKQLSEPIRSHKDL 1010
>TC88956 similar to PIR|C84514|C84514 hypothetical protein At2g14110
[imported] - Arabidopsis thaliana, partial (80%)
Length = 836
Score = 27.7 bits (60), Expect = 1.1
Identities = 16/56 (28%), Positives = 27/56 (47%)
Frame = +1
Query: 5 NKCGSIAYFLSTLHYTTWTNTACSKAGITVAIEDIHSTYNFKSRNRSTGVAFSAAL 60
NK G ++F++ Y++WT H T +F+ + +TGV FS+ L
Sbjct: 250 NKLGITSFFVAQEIYSSWT----------------HKTDHFQKIHSATGVPFSSML 369
>BQ144458 GP|21740724|em OSJNBa0086B14.17 {Oryza sativa}, partial (4%)
Length = 1364
Score = 26.6 bits (57), Expect = 2.5
Identities = 8/15 (53%), Positives = 12/15 (79%)
Frame = -2
Query: 107 AVYTSKLLRGCRCVC 121
A YT+++ RGC C+C
Sbjct: 1264 AAYTARI*RGCACIC 1220
>TC77439 similar to PIR|H84788|H84788 probable peroxidase ATP2a [imported] -
Arabidopsis thaliana, partial (68%)
Length = 867
Score = 25.8 bits (55), Expect = 4.3
Identities = 17/45 (37%), Positives = 22/45 (48%), Gaps = 7/45 (15%)
Frame = -2
Query: 10 IAYFL---STLHYTTWT----NTACSKAGITVAIEDIHSTYNFKS 47
IA+F STLH+ WT NT S+ V+ T+ FKS
Sbjct: 440 IAFFAILSSTLHFKVWTSQPGNTVSSRKSNNVSA*HCQRTFFFKS 306
>TC76392 similar to GP|14030701|gb|AAK53025.1 At2g28370/T1B3.11 {Arabidopsis
thaliana}, partial (55%)
Length = 918
Score = 25.8 bits (55), Expect = 4.3
Identities = 15/45 (33%), Positives = 23/45 (50%)
Frame = +1
Query: 26 ACSKAGITVAIEDIHSTYNFKSRNRSTGVAFSAALGLLECCFVNT 70
AC+ AGITV I++ + + N + V F A G+ C+ T
Sbjct: 544 ACASAGITVLIDN-----DLGNCNENHCVQFETATGMAFICWFTT 663
>TC88944 type IIB calcium ATPase [Medicago truncatula]
Length = 1883
Score = 25.8 bits (55), Expect = 4.3
Identities = 24/86 (27%), Positives = 39/86 (44%)
Frame = +3
Query: 2 SLSNKCGSIAYFLSTLHYTTWTNTACSKAGITVAIEDIHSTYNFKSRNRSTGVAFSAALG 61
S + C L+T H T C K I++++S+ + S + S+ + SA
Sbjct: 108 SATTICSDKTGTLTTNHMTVVKACICGK------IKEVNSSID--SSDFSSDLPDSAIAI 263
Query: 62 LLECCFVNTGSRIMVFASGPGLVLDS 87
LLE F NTG ++ +G +L S
Sbjct: 264 LLESIFNNTGGEVVKNENGKIEILGS 341
>TC76379 similar to GP|14030701|gb|AAK53025.1 At2g28370/T1B3.11 {Arabidopsis
thaliana}, partial (94%)
Length = 755
Score = 25.8 bits (55), Expect = 4.3
Identities = 15/45 (33%), Positives = 23/45 (50%)
Frame = +2
Query: 26 ACSKAGITVAIEDIHSTYNFKSRNRSTGVAFSAALGLLECCFVNT 70
AC+ AGITV I++ + + N + V F A G+ C+ T
Sbjct: 575 ACASAGITVLIDN-----DLGNCNENHCVQFETATGMAFICWFTT 694
>TC90070 similar to PIR|A90125|A90125 60S ribosomal protein L18A [imported]
- Guillardia theta nucleomorph, partial (9%)
Length = 318
Score = 25.8 bits (55), Expect = 4.3
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -3
Query: 16 TLHYTTWTNTACSKAGITVAIEDIHSTYNFKSRNRSTGVAF 56
T Y +WT+T+CS+ +++++ S Y K + T F
Sbjct: 292 TREYWSWTSTSCSRILVSLSL----SIYQIKQEDGITSNVF 182
>TC81479 similar to GP|15215660|gb|AAK91375.1 At1g15870/F7H2_19 {Arabidopsis
thaliana}, partial (38%)
Length = 858
Score = 25.8 bits (55), Expect = 4.3
Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 14/48 (29%)
Frame = -2
Query: 18 HYTTWTNTACSKAGITVAIEDI--------------HSTYNFKSRNRS 51
HYT W + A IT+ + DI H+T F+SRN S
Sbjct: 479 HYTVWPSRAHDFQHITIFLGDIDN*GNV*LHFICISHTTAFFRSRNSS 336
>AL384478 weakly similar to PIR|T39353|T393 probable splicing factor -
fission yeast (Schizosaccharomyces pombe), partial (26%)
Length = 499
Score = 25.0 bits (53), Expect = 7.3
Identities = 10/28 (35%), Positives = 18/28 (63%)
Frame = -3
Query: 1 MSLSNKCGSIAYFLSTLHYTTWTNTACS 28
++L+NKC + +LS + T +T+CS
Sbjct: 461 INLTNKCTELTAYLSLIKLFTRFHTSCS 378
>TC92661 similar to GP|22093674|dbj|BAC06968. kinase-like protein {Oryza
sativa (japonica cultivar-group)}, partial (13%)
Length = 702
Score = 25.0 bits (53), Expect = 7.3
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = +3
Query: 6 KCGSIAYFLSTLHYTTWTNTACSKAGITV 34
KCG+ +FLS+ H CS+A + V
Sbjct: 393 KCGTHLFFLSSFHLKGLFVMQCSEAALRV 479
>AL387173
Length = 436
Score = 25.0 bits (53), Expect = 7.3
Identities = 14/55 (25%), Positives = 24/55 (43%)
Frame = -3
Query: 3 LSNKCGSIAYFLSTLHYTTWTNTACSKAGITVAIEDIHSTYNFKSRNRSTGVAFS 57
++ + F +++ + N + + G T D+HST S STG A S
Sbjct: 227 VTERSSKFKQFQNSILFLFSLNCSVTFEGNTTDFSDLHSTIRVSSDEGSTGTARS 63
>TC86934 similar to PIR|T04630|T04630 synaptobrevin homolog F10N7.40 -
Arabidopsis thaliana, complete
Length = 1208
Score = 25.0 bits (53), Expect = 7.3
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +3
Query: 99 IYICNGHLAVYTSKLLRGCRCVC 121
I+ C G+ +++ +LL GC+ VC
Sbjct: 843 IFNCGGNYHLFSFQLLLGCQHVC 911
>TC76421 homologue to PIR|T02995|T02995 unspecific monooxygenase (EC
1.14.14.1) - common tobacco, partial (44%)
Length = 5080
Score = 25.0 bits (53), Expect = 7.3
Identities = 16/44 (36%), Positives = 22/44 (49%)
Frame = -3
Query: 47 SRNRSTGVAFSAALGLLECCFVNTGSRIMVFASGPGLVLDSDFW 90
S N GV SA LG + V+ S +++ +GP LDS W
Sbjct: 3422 STNTLCGV*VSAQLGTVTQLPVHPASPVLLTKNGPLGALDSMAW 3291
>CB891727 similar to GP|10716949|gb| SKP1 interacting partner 2 {Arabidopsis
thaliana}, partial (14%)
Length = 672
Score = 25.0 bits (53), Expect = 7.3
Identities = 16/44 (36%), Positives = 22/44 (49%)
Frame = -3
Query: 47 SRNRSTGVAFSAALGLLECCFVNTGSRIMVFASGPGLVLDSDFW 90
S N GV SA LG + V+ S +++ +GP LDS W
Sbjct: 337 STNTLCGV*VSAQLGTVTQLPVHPASPVLLTKNGPLGALDSMAW 206
>TC76312 GP|8118527|gb|AAF73016.1| ubiquitin conjugating protein {Avicennia
marina}, complete
Length = 1369
Score = 25.0 bits (53), Expect = 7.3
Identities = 16/44 (36%), Positives = 22/44 (49%)
Frame = +1
Query: 47 SRNRSTGVAFSAALGLLECCFVNTGSRIMVFASGPGLVLDSDFW 90
S N GV SA LG + V+ S +++ +GP LDS W
Sbjct: 544 STNTLCGV*VSAQLGTVTQLPVHPASPVLLTKNGPLGALDSMAW 675
>AW692464
Length = 654
Score = 25.0 bits (53), Expect = 7.3
Identities = 16/44 (36%), Positives = 22/44 (49%)
Frame = -1
Query: 47 SRNRSTGVAFSAALGLLECCFVNTGSRIMVFASGPGLVLDSDFW 90
S N GV SA LG + V+ S +++ +GP LDS W
Sbjct: 189 STNTLCGV*VSAQLGTVTQLPVHPASPVLLTKNGPLGALDSMAW 58
>BG450380 similar to PIR|T10971|T10 Pv42 protein - kidney bean, partial (36%)
Length = 571
Score = 24.6 bits (52), Expect = 9.6
Identities = 11/19 (57%), Positives = 12/19 (62%), Gaps = 2/19 (10%)
Frame = +2
Query: 15 STLHYTTWTNT--ACSKAG 31
ST+H TWTN CSK G
Sbjct: 173 STIHSFTWTNNEHTCSKQG 229
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.135 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,875,237
Number of Sequences: 36976
Number of extensions: 65827
Number of successful extensions: 509
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 504
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 507
length of query: 122
length of database: 9,014,727
effective HSP length: 84
effective length of query: 38
effective length of database: 5,908,743
effective search space: 224532234
effective search space used: 224532234
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146683.3