
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146681.9 + phase: 2 /pseudo
(249 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM779095 weakly similar to PIR|B84431|B8 probable receptor prote... 30 0.87
TC86171 weakly similar to GP|13507547|gb|AAK28636.1 unknown prot... 30 0.87
TC87983 similar to SP|Q10128|YSO2_CAEEL Hypothetical 81.6 kDa pr... 29 1.5
TC80652 similar to GP|14161088|emb|CAB76907. alpha-fucosidase {C... 29 1.9
TC80264 similar to GP|21689699|gb|AAM67471.1 putative F-box fami... 29 1.9
TC82409 similar to GP|13249030|gb|AAK16647.1 F-box containing pr... 28 2.5
AW689938 weakly similar to GP|22296343|db similar to leucine-ric... 27 5.6
TC86614 similar to PIR|T06308|T06308 protein phosphatase 2C homo... 27 7.4
TC88413 weakly similar to GP|10176786|dbj|BAB09900. gene_id:MIK1... 27 7.4
TC85262 similar to GP|9279671|dbj|BAB01228.1 transport inhibitor... 27 7.4
TC90163 similar to SP|Q9U6A1|PRO_DROVI Protein prospero. [Fruit ... 27 9.6
>BM779095 weakly similar to PIR|B84431|B8 probable receptor protein kinase
[imported] - Arabidopsis thaliana, partial (15%)
Length = 721
Score = 30.0 bits (66), Expect = 0.87
Identities = 32/99 (32%), Positives = 48/99 (48%), Gaps = 6/99 (6%)
Frame = +3
Query: 82 RLACLKLEACDLSCDVVNHLLDSLPVLNG---TLKSLSIAENCLGSKVAGALGRFLSTPI 138
++ C L DLS N+L DS P+ +LKSL++A N + + ALG+ +
Sbjct: 138 KIDCKSLLQLDLSG---NNLSDSFPISLSNCTSLKSLNLASNFISGGIPKALGQL--NKL 302
Query: 139 EVLDASGIDL---LPSGFLELQNMLTIEEELSLVIINIS 174
+ LD S + +PS EL N+ EL L NI+
Sbjct: 303 QSLDLSHNQITGWIPS---ELSNVCGSLLELKLSFNNIT 410
>TC86171 weakly similar to GP|13507547|gb|AAK28636.1 unknown protein
{Arabidopsis thaliana}, partial (89%)
Length = 2343
Score = 30.0 bits (66), Expect = 0.87
Identities = 32/116 (27%), Positives = 50/116 (42%), Gaps = 1/116 (0%)
Frame = +1
Query: 49 PNLEDLDISDNPIEDEGLRYLIPYFAGTSEMCSRLACLKLEACDLSCDVVNHLLDSLPVL 108
P+L +D+S + + D GLR L + C L L L CD H L L L
Sbjct: 619 PSLLAVDVSGSNVTDHGLRLL--------KDCLNLQALTLNYCD---QFSEHGLKHLSGL 765
Query: 109 NGTLKSLSIAENCLGSKVAGALGRFLS-TPIEVLDASGIDLLPSGFLELQNMLTIE 163
+ L SLSI ++C + + F + +E LD + GF+ + + +E
Sbjct: 766 S-NLTSLSIRKSC--AVTPDGMRAFSNLVNLEKLDLERCSDIHGGFVHFKGLKKLE 924
Score = 28.9 bits (63), Expect = 1.9
Identities = 17/54 (31%), Positives = 27/54 (49%)
Frame = +1
Query: 48 MPNLEDLDISDNPIEDEGLRYLIPYFAGTSEMCSRLACLKLEACDLSCDVVNHL 101
M L L++S++ + +EGLRYL P L L LE+C ++ + L
Sbjct: 1780 MTALRSLNVSNSRVTNEGLRYLKP--------LKNLRTLSLESCKVNAADIKKL 1917
>TC87983 similar to SP|Q10128|YSO2_CAEEL Hypothetical 81.6 kDa protein
F56D1.2 in chromosome II precursor. {Caenorhabditis
elegans}, partial (2%)
Length = 745
Score = 29.3 bits (64), Expect = 1.5
Identities = 18/58 (31%), Positives = 27/58 (46%)
Frame = -3
Query: 64 EGLRYLIPYFAGTSEMCSRLACLKLEACDLSCDVVNHLLDSLPVLNGTLKSLSIAENC 121
E L ++ PY+ G + C L L V +HLL +P +N SL I+ +C
Sbjct: 692 EILAFICPYYFGYNNDIKL*ICFHLIK*TLFSCVASHLLLLVPCINSLALSLDISFHC 519
>TC80652 similar to GP|14161088|emb|CAB76907. alpha-fucosidase {Cicer
arietinum}, partial (81%)
Length = 788
Score = 28.9 bits (63), Expect = 1.9
Identities = 30/101 (29%), Positives = 40/101 (38%)
Frame = +2
Query: 40 NLGYAFAYMPNLEDLDISDNPIEDEGLRYLIPYFAGTSEMCSRLACLKLEACDLSCDVVN 99
NL AF+ + LDI+ NPI G Y++P G RL C+ V
Sbjct: 53 NLSLAFSNDAVEQVLDINGNPIFPGGKYYILPAIRGPLGGGLRLGKSSNSDCE-----VT 217
Query: 100 HLLDSLPVLNGTLKSLSIAENCLGSKVAGALGRFLSTPIEV 140
+ D V+NG SI E G F TPI++
Sbjct: 218 VVQDYNEVINGVPVKFSIPEISPGI-------IFTGTPIDI 319
>TC80264 similar to GP|21689699|gb|AAM67471.1 putative F-box family protein
AtFBL3 {Arabidopsis thaliana}, partial (4%)
Length = 1305
Score = 28.9 bits (63), Expect = 1.9
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +3
Query: 80 CSRLACLKLEACDLSCDV 97
C LACLKLE+CD+ ++
Sbjct: 645 CPNLACLKLESCDMVTEI 698
>TC82409 similar to GP|13249030|gb|AAK16647.1 F-box containing protein TIR1
{Populus tremula x Populus tremuloides}, partial (37%)
Length = 1038
Score = 28.5 bits (62), Expect = 2.5
Identities = 21/89 (23%), Positives = 40/89 (44%), Gaps = 5/89 (5%)
Frame = +2
Query: 36 DDAENLGYAFAYMPNLEDLDISDNPIEDEGLRYLIPYFAGTSEMCSRLACLKLEACDLSC 95
D +L Y PNL+ L+I D+P D LR + ++ + L + +C L+
Sbjct: 458 DTDNSLKYVLEGCPNLQKLEIRDSPFGDGALRSGLHHY-------YNMRFLWMSSCKLTR 616
Query: 96 DVVNHLLDSLP-----VLNGTLKSLSIAE 119
+ +LP V+N +++++ E
Sbjct: 617 QACQEVARALPQMVMEVINNDVEAVNDIE 703
>AW689938 weakly similar to GP|22296343|db similar to leucine-rich repeat
transmembrane protein kinase 1, partial (16%)
Length = 655
Score = 27.3 bits (59), Expect = 5.6
Identities = 13/31 (41%), Positives = 20/31 (63%)
Frame = +3
Query: 41 LGYAFAYMPNLEDLDISDNPIEDEGLRYLIP 71
LGY + + +L LD+SDN I D+ + Y +P
Sbjct: 339 LGYLLSDLMSLRKLDLSDNKIHDQ-IPYQLP 428
>TC86614 similar to PIR|T06308|T06308 protein phosphatase 2C homolog
F11C18.60 - Arabidopsis thaliana, partial (92%)
Length = 1556
Score = 26.9 bits (58), Expect = 7.4
Identities = 28/96 (29%), Positives = 39/96 (40%), Gaps = 11/96 (11%)
Frame = +3
Query: 108 LNGTLKSLSIAENCLGSKVAGALGRFLSTP-IEVLDASG-IDLLPSGFLELQNMLTIEEE 165
L +L S S + NC S A+G +LSTP E G D L G +Q E+
Sbjct: 120 LINSLFSSSFSINCSSSPQKIAMGIYLSTPKTEKFSEDGENDRLRYGLSSMQGWRASMED 299
Query: 166 LSLVIINISKN---------RGGIQTARFLSKLLPQ 192
++ K+ GG A+F +K L Q
Sbjct: 300 AHAAYTDLDKSTSFFGVYDGHGGKVVAKFCAKFLHQ 407
>TC88413 weakly similar to GP|10176786|dbj|BAB09900.
gene_id:MIK19.29~pir||T01344~similar to unknown protein
{Arabidopsis thaliana}, partial (10%)
Length = 1288
Score = 26.9 bits (58), Expect = 7.4
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -3
Query: 26 LSHRGNNLRKDDAENLGYAF 45
LSH+GN L+K DAE + F
Sbjct: 539 LSHKGNGLKKGDAERIVTVF 480
>TC85262 similar to GP|9279671|dbj|BAB01228.1 transport inhibitor
response-like protein {Arabidopsis thaliana}, partial
(47%)
Length = 1291
Score = 26.9 bits (58), Expect = 7.4
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3
Query: 95 CDVVNHLLDSLPVLNGTLKSLSIAEN 120
C + HLLDSLP+ + T+ + EN
Sbjct: 1031 CTIQIHLLDSLPIFHVTICLFTFTEN 954
>TC90163 similar to SP|Q9U6A1|PRO_DROVI Protein prospero. [Fruit fly]
{Drosophila virilis}, partial (1%)
Length = 951
Score = 26.6 bits (57), Expect = 9.6
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = -1
Query: 206 MPIESLSIISSALKFAKGIYGVPVSYIGDLLDLRCWQVVTSW 247
+PI LS + +L F I+ +PVSY LDL ++ TS+
Sbjct: 792 LPISILSYVPCSLTF---IHNLPVSYFSYSLDLVYYRGSTSY 676
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.330 0.146 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,912,541
Number of Sequences: 36976
Number of extensions: 107171
Number of successful extensions: 644
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 639
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 644
length of query: 249
length of database: 9,014,727
effective HSP length: 94
effective length of query: 155
effective length of database: 5,538,983
effective search space: 858542365
effective search space used: 858542365
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146681.9