BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146681.8 + phase: 0
(173 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF643539 weakly similar to GP|4512715|gb|A unknown protein {Arab... 236 8e-83
TC84479 ENBP1 30 0.37
TC91557 similar to GP|16604597|gb|AAL24155.1 unknown protein {Ar... 28 1.8
TC86520 similar to GP|11385463|gb|AAG34814.1 glutathione S-trans... 27 3.2
TC77162 similar to SP|Q42884|ARC1_LYCES Chorismate synthase 1 c... 27 3.2
AJ501098 homologue to GP|1871184|gb|A unknown protein {Arabidops... 27 5.4
TC83080 similar to PIR|E86445|E86445 hypothetical protein AAG234... 27 5.4
TC90581 similar to PIR|E86357|E86357 alcohol dehydrogenase homol... 27 5.4
TC86464 similar to GP|2098711|gb|AAB57670.1| pectinesterase {Cit... 27 5.4
AW587149 similar to PIR|B85431|B85 trichohyalin like protein [im... 27 5.4
BE326045 similar to PIR|F96753|F96 Similar to downy mildew resis... 26 7.0
TC87785 similar to GP|4204313|gb|AAD10694.1| lcl|prt_seq No defi... 26 7.0
TC78903 26 7.0
BQ151336 26 9.2
TC88884 26 9.2
TC91276 similar to GP|14190487|gb|AAK55724.1 AT3g59280/F25L23_14... 26 9.2
BI271537 similar to GP|19911209|db glucosyltransferase-13 {Vigna... 26 9.2
>BF643539 weakly similar to GP|4512715|gb|A unknown protein {Arabidopsis
thaliana}, partial (24%)
Length = 614
Score = 236 bits (602), Expect(2) = 8e-83
Identities = 112/112 (100%), Positives = 112/112 (100%)
Frame = +1
Query: 1 MVEEAPPSSLITLCIDHLANQLLFGEDEVIAIIPVIYELPSHLLDDLISRLTPHALYHFH 60
MVEEAPPSSLITLCIDHLANQLLFGEDEVIAIIPVIYELPSHLLDDLISRLTPHALYHFH
Sbjct: 52 MVEEAPPSSLITLCIDHLANQLLFGEDEVIAIIPVIYELPSHLLDDLISRLTPHALYHFH 231
Query: 61 LHMPFQDVNEEDFSRDDSTNNKRKRSRDWNLNTAWQKLFELRWPDLINQIQP 112
LHMPFQDVNEEDFSRDDSTNNKRKRSRDWNLNTAWQKLFELRWPDLINQIQP
Sbjct: 232 LHMPFQDVNEEDFSRDDSTNNKRKRSRDWNLNTAWQKLFELRWPDLINQIQP 387
Score = 87.4 bits (215), Expect(2) = 8e-83
Identities = 40/46 (86%), Positives = 43/46 (92%)
Frame = +2
Query: 109 QIQPSDWQKAYWEAHLQNCLDEAAEIALISPFKGRLADINISGTML 154
+ PSDWQKAYWEAHLQNCLDEAAEIALISPFKGRLADINIS ++L
Sbjct: 377 RFNPSDWQKAYWEAHLQNCLDEAAEIALISPFKGRLADINISESIL 514
>TC84479 ENBP1
Length = 5108
Score = 30.4 bits (67), Expect = 0.37
Identities = 15/55 (27%), Positives = 25/55 (45%), Gaps = 1/55 (1%)
Frame = -2
Query: 42 HLLDDLISRLTPHALYHFHLHMPFQDVNEEDFSRDDSTNNK-RKRSRDWNLNTAW 95
H D L H + HLH P D+N++ +S T+ + + +LN+ W
Sbjct: 529 HFHDHLYQHQKHHHHFQIHLHHPIADLNQQPYSYPQQTHQQIQLHHPKTDLNSHW 365
>TC91557 similar to GP|16604597|gb|AAL24155.1 unknown protein {Arabidopsis
thaliana}, partial (88%)
Length = 1217
Score = 28.1 bits (61), Expect = 1.8
Identities = 19/82 (23%), Positives = 41/82 (49%)
Frame = +1
Query: 41 SHLLDDLISRLTPHALYHFHLHMPFQDVNEEDFSRDDSTNNKRKRSRDWNLNTAWQKLFE 100
S ++++L+ +T HAL + ++ R TN+ +++ N + AW+ L+
Sbjct: 451 SSMMEELVPEITTHALSY---------LDYPSLCRLSMTNSLMRKAP--NDDNAWKALYH 597
Query: 101 LRWPDLINQIQPSDWQKAYWEA 122
+ + ++PS+ KAY+ A
Sbjct: 598 KDFTLEQDSVRPSNGWKAYYAA 663
>TC86520 similar to GP|11385463|gb|AAG34814.1 glutathione S-transferase GST
24 {Glycine max}, partial (98%)
Length = 1003
Score = 27.3 bits (59), Expect = 3.2
Identities = 14/48 (29%), Positives = 22/48 (45%), Gaps = 3/48 (6%)
Frame = +2
Query: 62 HMPFQDVNEEDFSRDDSTNNKRKRSRDWN---LNTAWQKLFELRWPDL 106
H+PF D + +D +K+ S WN +W K+ EL P +
Sbjct: 650 HLPFMDYVVNNMGKDYLIKDKKHVSAWWNDISSRPSWNKVLELYKPPI 793
>TC77162 similar to SP|Q42884|ARC1_LYCES Chorismate synthase 1 chloroplast
precursor (EC 4.6.1.4), partial (86%)
Length = 2021
Score = 27.3 bits (59), Expect = 3.2
Identities = 16/56 (28%), Positives = 26/56 (45%)
Frame = -2
Query: 42 HLLDDLISRLTPHALYHFHLHMPFQDVNEEDFSRDDSTNNKRKRSRDWNLNTAWQK 97
HLL +++ L P + YH + +P V F + +R+ DWN W+K
Sbjct: 535 HLLHEILHML*PESGYHTYSQLPEYGV---VFWGSEDGGRRRRGIEDWN----WEK 389
>AJ501098 homologue to GP|1871184|gb|A unknown protein {Arabidopsis
thaliana}, partial (15%)
Length = 625
Score = 26.6 bits (57), Expect = 5.4
Identities = 12/46 (26%), Positives = 24/46 (52%)
Frame = +3
Query: 53 PHALYHFHLHMPFQDVNEEDFSRDDSTNNKRKRSRDWNLNTAWQKL 98
PH +H++L P SR+ ST+ ++ ++++W+KL
Sbjct: 414 PHQNFHYYLSSPIH------HSRESSTSRYSASLKNPRISSSWKKL 533
>TC83080 similar to PIR|E86445|E86445 hypothetical protein AAG23445.1
[imported] - Arabidopsis thaliana, partial (11%)
Length = 1147
Score = 26.6 bits (57), Expect = 5.4
Identities = 22/60 (36%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Frame = +1
Query: 94 AWQKLFELRWPDLINQIQPSDWQKAYWEAHLQNCLDEAAEIALISPFKG--RLADINISG 151
+W +F L P I S ++ YW HL +CL E E FK +L INISG
Sbjct: 226 SWLYIFLL*GPVCI-----SLFEFIYWHKHLLDCLGELME-TTYDMFKTCFQLISINISG 387
>TC90581 similar to PIR|E86357|E86357 alcohol dehydrogenase homolog [imported]
- Arabidopsis thaliana, partial (52%)
Length = 1289
Score = 26.6 bits (57), Expect = 5.4
Identities = 13/29 (44%), Positives = 19/29 (64%)
Frame = -1
Query: 35 VIYELPSHLLDDLISRLTPHALYHFHLHM 63
+I L S+L+ +ISRL PH+LY H+
Sbjct: 1142 IILGLLSNLI*HIISRLLPHSLYKSLNHL 1056
>TC86464 similar to GP|2098711|gb|AAB57670.1| pectinesterase {Citrus
sinensis}, partial (85%)
Length = 1993
Score = 26.6 bits (57), Expect = 5.4
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = -3
Query: 131 AAEIALISPFKGRLADINISGTMLSCFLCNEIFNAKLP 168
AA + ++PF+ L ++ G + L ++FNAKLP
Sbjct: 1730 AALVMTLNPFQVTLLEVPAPGPVF*YSL*TKVFNAKLP 1617
>AW587149 similar to PIR|B85431|B85 trichohyalin like protein [imported] -
Arabidopsis thaliana, partial (1%)
Length = 634
Score = 26.6 bits (57), Expect = 5.4
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = -2
Query: 115 WQKAYWEAHLQNCLDEAAEIALISPFKGRLADINISGTMLSCFL 158
W+ WEA + CL E AL P K L S ++L CF+
Sbjct: 150 WR*VSWEARERLCLVGGDENALHFPLKKMLLG*G*SFSLLFCFV 19
>BE326045 similar to PIR|F96753|F96 Similar to downy mildew resistance
protein RPP5 [imported] - Arabidopsis thaliana, partial
(6%)
Length = 587
Score = 26.2 bits (56), Expect = 7.0
Identities = 11/33 (33%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +2
Query: 12 TLCIDHLANQLLFGEDEVIAIIPVIYEL-PSHL 43
T C+D L + + ++ I ++PV Y + PSH+
Sbjct: 353 TWCLDELTHMMKCLKNNQIVVVPVFYNVDPSHV 451
>TC87785 similar to GP|4204313|gb|AAD10694.1| lcl|prt_seq No definition
line found {Arabidopsis thaliana}, partial (38%)
Length = 1216
Score = 26.2 bits (56), Expect = 7.0
Identities = 10/31 (32%), Positives = 15/31 (48%)
Frame = +1
Query: 58 HFHLHMPFQDVNEEDFSRDDSTNNKRKRSRD 88
HFH H+ FQ F + N+ K +R+
Sbjct: 31 HFHFHLHFQ*SQSSQFQIPNHNGNRTKTTRE 123
>TC78903
Length = 332
Score = 26.2 bits (56), Expect = 7.0
Identities = 13/28 (46%), Positives = 18/28 (63%)
Frame = +3
Query: 84 KRSRDWNLNTAWQKLFELRWPDLINQIQ 111
KRSR+WNLN W+ +L +NQ+Q
Sbjct: 6 KRSREWNLND-WRWDGDLFIASRVNQVQ 86
>BQ151336
Length = 1045
Score = 25.8 bits (55), Expect = 9.2
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = -3
Query: 100 ELRWPDLINQIQPSDWQKAYWEAHLQNCLDEAAEI 134
++RWP ++ + S W Y L CL E +E+
Sbjct: 626 DIRWPIIVGYMAVSTWDSCYSRRSLL-CLFECSEL 525
>TC88884
Length = 1128
Score = 25.8 bits (55), Expect = 9.2
Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = -1
Query: 41 SHLLDDLISRLTPHAL-YHFHLHMPFQDVNEEDFSRDDSTNNKRK 84
S++ DD IS +TP + H P++D + ED + ++ N+RK
Sbjct: 1110 SYVEDDSISAVTPPFV*AKNSQHSPWEDKSGEDNNIKEALTNRRK 976
>TC91276 similar to GP|14190487|gb|AAK55724.1 AT3g59280/F25L23_140
{Arabidopsis thaliana}, partial (87%)
Length = 706
Score = 25.8 bits (55), Expect = 9.2
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = -1
Query: 144 LADINISGTMLSCFLCNEIF 163
L +++ + ++L CFLCN IF
Sbjct: 310 LCNLSFADSILYCFLCNAIF 251
>BI271537 similar to GP|19911209|db glucosyltransferase-13 {Vigna angularis},
partial (34%)
Length = 652
Score = 25.8 bits (55), Expect = 9.2
Identities = 20/50 (40%), Positives = 24/50 (48%), Gaps = 5/50 (10%)
Frame = -3
Query: 20 NQLLFGEDEVIAIIPVIYELPSHLLD---DLIS--RLTPHALYHFHLHMP 64
N LL + I +IP Y SHLL D S + P L HFH H+P
Sbjct: 641 NHLL*QHSKQILLIPSCYCAISHLLPLP*DT*SP*QPLPFPLCHFH*HLP 492
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.139 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,124,937
Number of Sequences: 36976
Number of extensions: 109550
Number of successful extensions: 723
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 719
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 723
length of query: 173
length of database: 9,014,727
effective HSP length: 89
effective length of query: 84
effective length of database: 5,723,863
effective search space: 480804492
effective search space used: 480804492
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146681.8