BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146651.18 + phase: 0 /pseudo
(559 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF648394 175 1e-80
TC82456 weakly similar to GP|18057159|gb|AAL58182.1 putative far... 65 9e-11
AW687028 weakly similar to GP|9909176|dbj| hypothetical protein~... 55 5e-08
BG449141 similar to GP|15983442|gb At1g10240/F14N23_12 {Arabidop... 49 4e-06
TC89654 weakly similar to PIR|T05644|T05644 hypothetical protein... 40 0.003
TC83834 similar to PIR|G96565|G96565 F6D8.26 [imported] - Arabid... 37 0.015
BG646905 weakly similar to GP|20804419|dbj contains EST C73272(E... 33 0.22
BQ137028 similar to GP|4914334|gb|A F14N23.20 {Arabidopsis thali... 31 1.1
TC79642 similar to PIR|T07397|T07397 kinesin heavy chain-like pr... 30 1.9
TC78449 similar to GP|21555695|gb|AAM63916.1 unknown {Arabidopsi... 30 2.4
TC89357 similar to GP|20145851|emb|CAD29616. auxin response fact... 30 2.4
TC85081 similar to GP|21240658|gb|AAM44369.1 putative protein co... 30 3.2
AL369214 30 3.2
BG456490 weakly similar to GP|22296361|dbj AHM1(AT hook-containi... 30 3.2
BE203031 similar to PIR|T04868|T048 hypothetical protein F28A21.... 30 3.2
BI308227 similar to GP|17978937|gb AT4g32850/T16I18_60 {Arabidop... 29 4.2
TC80505 29 5.5
TC85211 PIR|S22489|S22489 IgE-dependent histamine-releasing fact... 29 5.5
TC78624 similar to GP|4850385|gb|AAD31055.1| EST gb|T22808 comes... 29 5.5
TC82553 similar to PIR|T00951|T00951 probable 3-oxoacyl-[acyl-ca... 28 7.1
>BF648394
Length = 650
Score = 175 bits (444), Expect(2) = 1e-80
Identities = 94/119 (78%), Positives = 99/119 (82%), Gaps = 8/119 (6%)
Frame = -3
Query: 280 VESAHALLKKYLDNSVGDLGTFWEKIHDMLLLQFTAIQTTFGQSVTVLEHRFKDVILYSD 339
VESA LLKKYLDNSVGDLGT WEKIHDMLLLQF AIQTTFGQSV+VLEHRFK V LYS
Sbjct: 648 VESAXDLLKKYLDNSVGDLGTCWEKIHDMLLLQFIAIQTTFGQSVSVLEHRFKYVTLYSG 469
Query: 340 LGGHVSRYALDNSALEETRCRETLCIENNI---VQRTSYGLPC-----A*LLEEKPILL 390
+GGHVSRYALDN ALEET CR+TLC+ N+I VQRTSYGLPC LLEEKPILL
Sbjct: 468 IGGHVSRYALDNIALEETHCRKTLCMYNDI*GCVQRTSYGLPCPCEIATKLLEEKPILL 292
Score = 143 bits (360), Expect(2) = 1e-80
Identities = 70/81 (86%), Positives = 76/81 (93%)
Frame = -2
Query: 390 LDELLHH*HRLRMGEESNEVGFCVKVELKAIVEHIKKLLFQMKLEVKEGLRQLTFSETTM 449
+DE+ HH +LRMGEESNEVGFCV+VELKAIVEH+KKL FQMK+EVKEGLRQLTF ETTM
Sbjct: 295 IDEIYHHWLKLRMGEESNEVGFCVEVELKAIVEHLKKLPFQMKVEVKEGLRQLTFPETTM 116
Query: 450 MSPPPRKVPTKGAKKKVDIAR 470
MSPPPRKVPTKGAKKKVDIAR
Sbjct: 115 MSPPPRKVPTKGAKKKVDIAR 53
>TC82456 weakly similar to GP|18057159|gb|AAL58182.1 putative far-red
impaired response protein {Oryza sativa}, partial (7%)
Length = 1244
Score = 64.7 bits (156), Expect = 9e-11
Identities = 36/152 (23%), Positives = 71/152 (46%), Gaps = 1/152 (0%)
Frame = +2
Query: 36 RGDKKSLQYLISKLEEHNYTYY-SRTQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTY 94
RGD ++ ++++ N +Y + + I+++FWA + F V+ +D+TY
Sbjct: 710 RGDVDAIHSFFHRMQKQNSQFYCAMDMDDKRNIQNLFWADARCRAAYEYFGEVITLDTTY 889
Query: 95 KTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILRKLLTSKMNMPKVIVT 154
T+ Y + + VGV T +G +++ + W+ R L + P I+T
Sbjct: 890 LTSKYDLPLVPFVGVNHHGQTILLGCAILSNLDAKTLTWL--FTRWLECMHGHAPNGIIT 1063
Query: 155 DRDMSLMKAVENIFPKSYTMNCYFHVQANVKQ 186
+ D ++ A+E FPK+ C +H+ V +
Sbjct: 1064EEDKAMKNAIEVAFPKARHRWCLWHIMKKVPE 1159
>AW687028 weakly similar to GP|9909176|dbj| hypothetical protein~similar to
Arabidopsis thaliana F20D10.300, partial (11%)
Length = 662
Score = 55.5 bits (132), Expect = 5e-08
Identities = 30/100 (30%), Positives = 49/100 (49%)
Frame = +1
Query: 87 VLVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILRKLLTSKM 146
VL D+TYK N Y + G T G + E E++ WVL + + +K
Sbjct: 4 VLAFDTTYKKNKYNYPLCIFSGCNHHSQTIIFGVALLEDETIESYKWVLNRFLECMENKF 183
Query: 147 NMPKVIVTDRDMSLMKAVENIFPKSYTMNCYFHVQANVKQ 186
PK +VTD D S+ +A++ +FP + C +H+ N ++
Sbjct: 184 --PKAVVTDGDGSMREAIKQVFPDASHRLCAWHLHKNAQE 297
>BG449141 similar to GP|15983442|gb At1g10240/F14N23_12 {Arabidopsis
thaliana}, partial (33%)
Length = 690
Score = 49.3 bits (116), Expect = 4e-06
Identities = 34/163 (20%), Positives = 77/163 (46%), Gaps = 2/163 (1%)
Frame = +3
Query: 22 KQVYNERQQIRKANRGDKK-SLQYLISKLEEHNYTY-YSRTQSESTTIEDIFWAHPTFVK 79
K V N Q RK + ++ L + +++ + + + T + +E+I W++ + ++
Sbjct: 180 KDVRNLLQSFRKLDPEEETLDLLRMCRNIKDKDPNFKFEYTLDANNRLENIAWSYASSIQ 359
Query: 80 LFNNFPTVLVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILR 139
L++ F +V D+T++ + M + VG+ + + G + E +F W +K
Sbjct: 360 LYDIFGDAVVFDTTHRLTAFDMPLGLWVGINNYGMPCFFGCVLLRDETVRSFSWAIKAFL 539
Query: 140 KLLTSKMNMPKVIVTDRDMSLMKAVENIFPKSYTMNCYFHVQA 182
+ K P+ I+TD+++ L +A+ P + C + + A
Sbjct: 540 GFMNGK--APQTILTDQNICLKEALSAEMPMTKHAFCIWMIVA 662
>TC89654 weakly similar to PIR|T05644|T05644 hypothetical protein F20D10.290
- Arabidopsis thaliana, partial (65%)
Length = 1358
Score = 39.7 bits (91), Expect = 0.003
Identities = 25/88 (28%), Positives = 43/88 (48%), Gaps = 1/88 (1%)
Frame = +2
Query: 15 PHCMTNVKQVYNERQQIRKANRGDKKSLQYLISKLEEHNYTYYSRTQSESTTIE-DIFWA 73
P+C + + RQ R G + L YL ++ N+ ++ QS+ +IFWA
Sbjct: 59 PNCCKL*AHMSSTRQ--RTLGGGGHQVLDYL-KRMRAENHAFFYAVQSDVDNAGGNIFWA 229
Query: 74 HPTFVKLFNNFPTVLVMDSTYKTNMYKM 101
T ++ F ++ D+TYKTN Y++
Sbjct: 230 DETCRTNYSYFGDTVIFDTTYKTNQYRV 313
>TC83834 similar to PIR|G96565|G96565 F6D8.26 [imported] - Arabidopsis
thaliana, partial (24%)
Length = 1032
Score = 37.4 bits (85), Expect = 0.015
Identities = 22/113 (19%), Positives = 51/113 (44%), Gaps = 1/113 (0%)
Frame = +3
Query: 25 YNERQQIRKANRGDKKSLQYLISKLEEHNYTY-YSRTQSESTTIEDIFWAHPTFVKLFNN 83
Y+ + +RK GD +++ + +++ N + Y ++ + + W
Sbjct: 660 YSNKLNLRK---GDTQAIYNFLCRMQLTNPNFFYLMDFNDEGHLRNALWVDAKSRAACGY 830
Query: 84 FPTVLVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLK 136
F V+ D+TY N Y++ + +VG+ + +G G + E E++ W+ +
Sbjct: 831 FSDVIYFDNTYLVNKYEIPLVALVGINHHGQSVLLGCGLLAGEIIESYKWLFR 989
>BG646905 weakly similar to GP|20804419|dbj contains EST
C73272(E3606)~similar to Arabidopsis thaliana chromosome
5 MZF18.9~unknown protein, partial (10%)
Length = 735
Score = 33.5 bits (75), Expect = 0.22
Identities = 17/46 (36%), Positives = 26/46 (55%), Gaps = 2/46 (4%)
Frame = +2
Query: 494 KDVRLGKTSLNPLSPPFRYPKPKTILVMR--PIDYMSRYMLPFIEK 537
K V +G S P+ P PKP +L++ +D+M +M P+IEK
Sbjct: 527 KSVCIGNPSPTPVPRPTPVPKPTMVLMLYIPHVDWMPLFMCPYIEK 664
>BQ137028 similar to GP|4914334|gb|A F14N23.20 {Arabidopsis thaliana},
partial (7%)
Length = 687
Score = 31.2 bits (69), Expect = 1.1
Identities = 29/126 (23%), Positives = 51/126 (40%), Gaps = 1/126 (0%)
Frame = +2
Query: 384 EEKPILLDEL-LHH*HRLRMGEESNEVGFCVKVELKAIVEHIKKLLFQMKLEVKEGLRQL 442
E K ILL++ R R+ E E + +K + + +E ++ L Q LE E +
Sbjct: 164 ERKRILLEQQEAERIERDRIAFEEREKAWIIKQQQQRDLEEEQQQLSQRDLEQHESELEE 343
Query: 443 TFSETTMMSPPPRKVPTKGAKKKVDIARLLIPKIRIVNRHHHQQHHHIKEKKDVRLGKTS 502
E R + + + P + + N +HH HHH +E+ + + +
Sbjct: 344 EEEEDVDDDDDGRPEIIWLGNEIIIQKKNPNPLLLLHNHNHHHHHHHHQEQHEHHDNRPT 523
Query: 503 LNPLSP 508
NP P
Sbjct: 524 SNPFPP 541
>TC79642 similar to PIR|T07397|T07397 kinesin heavy chain-like protein (clone
PKCBP) - potato, partial (42%)
Length = 2321
Score = 30.4 bits (67), Expect = 1.9
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -3
Query: 472 LIPKIRIVNRHHHQQHHHIKEKKDVRLGK 500
L P+I+ + HHHQ+H HI D L +
Sbjct: 1149 LYPQIQFLEWHHHQKHDHIYTVLDYHLSR 1063
>TC78449 similar to GP|21555695|gb|AAM63916.1 unknown {Arabidopsis
thaliana}, partial (70%)
Length = 1031
Score = 30.0 bits (66), Expect = 2.4
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 477 RIVNRHHHQQHHHIKEKKDVRLGKTSLN 504
R +++HHHQ H + K+V +G S N
Sbjct: 288 RDLHQHHHQHHQAVTNHKNVEIGTLSYN 371
>TC89357 similar to GP|20145851|emb|CAD29616. auxin response factor 36
{Arabidopsis thaliana}, partial (21%)
Length = 843
Score = 30.0 bits (66), Expect = 2.4
Identities = 17/57 (29%), Positives = 27/57 (46%), Gaps = 5/57 (8%)
Frame = +3
Query: 68 EDIFWAHPTFVKL-----FNNFPTVLVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVG 119
EDI+W H F+ N+F L + T+ N+ K L VV +T+++G
Sbjct: 186 EDIYWTHFQFIHFTQFLRSNDFDQQLALPKTFSNNVKKKLPENVVLKGPGGVTWNIG 356
>TC85081 similar to GP|21240658|gb|AAM44369.1 putative protein contains
similarity to topoisomerase-related function protein,
partial (1%)
Length = 823
Score = 29.6 bits (65), Expect = 3.2
Identities = 30/133 (22%), Positives = 62/133 (46%), Gaps = 10/133 (7%)
Frame = +3
Query: 413 VKVELKAIVEHIKKLLFQMKLEVKEGLRQLTFSETT-MMSPP---------PRKVPTKGA 462
V +E + +I + LF +++ K GL + F + +++ P PR+ P
Sbjct: 333 VGLEEFLVFTYIGENLFNVEIYGKNGLEKPCFKKKQEVVAAPIVAKRKRGRPRRNPAGVE 512
Query: 463 KKKVDIARLLIPKIRIVNRHHHQQHHHIKEKKDVRLGKTSLNPLSPPFRYPKPKTILVMR 522
++K + A ++ K ++V R + + KEK + T + P + P P ++++
Sbjct: 513 EEKEEAAPTIVAKTKMVQRKYPSRVCVRKEKAETL--ATIVTKKGIPRKNPAPLSVIL-- 680
Query: 523 PIDYMSRYMLPFI 535
+D ++YML I
Sbjct: 681 -VD*YNKYMLSVI 716
>AL369214
Length = 406
Score = 29.6 bits (65), Expect = 3.2
Identities = 19/77 (24%), Positives = 32/77 (40%), Gaps = 11/77 (14%)
Frame = -1
Query: 428 LFQMKLEVKEGLRQLTFSETTMMSPPPRKVPTKGAKKKVDI---ARLLIPKIRIVN---- 480
LF+ +++ ++ TF T ++ + K K + D+ +I VN
Sbjct: 379 LFKQVFFIQKNIQVFTFHTTKILILTNKNANMKKQK*ESDLNSEQHTMITNFSCVNVLLE 200
Query: 481 ----RHHHQQHHHIKEK 493
+HHH HHH EK
Sbjct: 199 SCSWKHHHHNHHHFLEK 149
>BG456490 weakly similar to GP|22296361|dbj AHM1(AT hook-containing MAR
binding protein1)-like protein {Oryza sativa (japonica
cultivar-group)}, partial (6%)
Length = 531
Score = 29.6 bits (65), Expect = 3.2
Identities = 16/59 (27%), Positives = 25/59 (42%)
Frame = -1
Query: 480 NRHHHQQHHHIKEKKDVRLGKTSLNPLSPPFRYPKPKTILVMRPIDYMSRYMLPFIEKM 538
N HHHQ+H I + +PLS R+ KT + P Y + P + ++
Sbjct: 483 NHHHHQKHKQI---------QLHFHPLSSHHRFSLRKTSTALLPYTYNTASSPPLLRRL 334
>BE203031 similar to PIR|T04868|T048 hypothetical protein F28A21.170 -
Arabidopsis thaliana, partial (6%)
Length = 345
Score = 29.6 bits (65), Expect = 3.2
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = +1
Query: 467 DIARLLIPKIRIVNRHHHQQHHHIKEKKDVRLGKTSLNPLSPPFRY 512
D+ LL+P I++ N HHH + +S PLSP F Y
Sbjct: 70 DLLVLLLPIIKL*NHHHHLHYFLFSSSP----SSSSSPPLSPQFLY 195
>BI308227 similar to GP|17978937|gb AT4g32850/T16I18_60 {Arabidopsis
thaliana}, partial (27%)
Length = 761
Score = 29.3 bits (64), Expect = 4.2
Identities = 29/117 (24%), Positives = 51/117 (42%), Gaps = 24/117 (20%)
Frame = +1
Query: 260 KIVRAWTDHVLHLGCRTSNMVESAHALLKKYLDNSVG------DLGT------------- 300
+IV++W H+ L T MVE A+A++ + +G DL T
Sbjct: 271 QIVKSWVKHLTRLKGYTDQMVEDANAVILTFGSYRLGVHGPGADLDTLCIGPSYVNREED 450
Query: 301 FWEKIHDML--LLQFTAIQTTFGQSVTVLEHRFKDV---ILYSDLGGHVSRYALDNS 352
F+ HD+L + + T +Q V V++ +F + +LY+ + + LD S
Sbjct: 451 FFYTFHDILASMDEVTELQPVPDAHVPVMKFKFDGISIDLLYASISCLIVPEELDIS 621
>TC80505
Length = 675
Score = 28.9 bits (63), Expect = 5.5
Identities = 24/81 (29%), Positives = 33/81 (40%), Gaps = 7/81 (8%)
Frame = +1
Query: 52 HNYTYYSRTQ-------SESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTYKTNMYKMLMF 104
HN+ + R SES F H ++ + NFPT ++S K N+Y L F
Sbjct: 262 HNFPSFIRIHYSLKPYPSESNNFSFSFLFHK*KLQKYTNFPTPFSINSPPKPNIYFFLSF 441
Query: 105 EVVGVTSTDLTYSVGFGFVTH 125
V + VGFG H
Sbjct: 442 LV*DL-------RVGFGTCHH 483
>TC85211 PIR|S22489|S22489 IgE-dependent histamine-releasing factor homolog
- alfalfa (fragment), partial (45%)
Length = 416
Score = 28.9 bits (63), Expect = 5.5
Identities = 15/29 (51%), Positives = 19/29 (64%), Gaps = 3/29 (10%)
Frame = +1
Query: 340 LGGHVSRYAL---DNSALEETRCRETLCI 365
LG HV R+ L +N+A +TRCRET I
Sbjct: 4 LGSHVCRFRLRGGNNTADTQTRCRETRAI 90
>TC78624 similar to GP|4850385|gb|AAD31055.1| EST gb|T22808 comes from this
gene. {Arabidopsis thaliana}, partial (51%)
Length = 2052
Score = 28.9 bits (63), Expect = 5.5
Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 3/47 (6%)
Frame = -3
Query: 480 NRHHHQQHHHIKEKKDV-RLGKTSLNPLSPPFRYP--KPKTILVMRP 523
N HHH HHI D +T+ SP YP +P T+ + +P
Sbjct: 724 NHHHHHHCHHIDSDSDSDDRNQTTEATSSPTPNYPPHQPSTVQIPKP 584
>TC82553 similar to PIR|T00951|T00951 probable
3-oxoacyl-[acyl-carrier-protein] synthase (EC 2.3.1.41)
F20D22.1 - Arabidopsis thaliana, partial (65%)
Length = 1343
Score = 28.5 bits (62), Expect = 7.1
Identities = 22/79 (27%), Positives = 33/79 (40%), Gaps = 6/79 (7%)
Frame = +2
Query: 422 EHIKKLLF---QMKLEVKEGLRQLTFSETTMMS---PPPRKVPTKGAKKKVDIARLLIPK 475
EH KL+F Q+ K L S + + PPP K+PT + R I +
Sbjct: 320 EHNTKLVFRQRQINASAKLSL*NGRSSSSPLQQATRPPPIKIPTSPHRPNAQRCRQQILR 499
Query: 476 IRIVNRHHHQQHHHIKEKK 494
+R+ R H+ I K+
Sbjct: 500 LRLSRRRRHKASRCITLKR 556
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.139 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,453,882
Number of Sequences: 36976
Number of extensions: 245680
Number of successful extensions: 3190
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 2610
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2961
length of query: 559
length of database: 9,014,727
effective HSP length: 101
effective length of query: 458
effective length of database: 5,280,151
effective search space: 2418309158
effective search space used: 2418309158
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146651.18