BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146651.13 - phase: 0
(274 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86627 similar to PIR|T02334|T02334 probable urease accessory p... 566 e-162
TC79517 similar to PIR|T08874|T08874 calcium-dependent protein k... 39 0.002
TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown prot... 36 0.018
BQ137028 similar to GP|4914334|gb|A F14N23.20 {Arabidopsis thali... 36 0.018
TC91060 similar to GP|18175803|gb|AAL59930.1 unknown protein {Ar... 35 0.031
TC93162 similar to PIR|G72453|G72453 hypothetical protein APE227... 35 0.040
TC90911 similar to PIR|T02681|T02681 probable zinc transporter A... 34 0.053
TC87570 homologue to GP|7208771|emb|CAB76908.1 putative Ruv DNA-... 34 0.069
TC86231 similar to GP|21593313|gb|AAM65262.1 protein disulfide i... 34 0.069
TC84261 similar to GP|18650616|gb|AAL75907.1 AT4g03420/F9H3_4 {A... 33 0.090
TC79991 similar to GP|10177307|dbj|BAB10568. contains similarity... 33 0.12
TC89559 similar to GP|20259581|gb|AAM14133.1 unknown protein {Ar... 33 0.12
TC77148 ENOD20 33 0.15
TC86933 homologue to GP|2674201|gb|AAC61786.1| glycine-rich RNA-... 33 0.15
TC80287 weakly similar to GP|12667516|gb|AAG61157.1 DBC2 {Homo s... 32 0.20
TC82470 homologue to GP|23497460|gb|AAN37003.1 hypothetical prot... 32 0.20
AJ388976 similar to PIR|E84638|E84 probable RSZp22 splicing fact... 32 0.20
TC86766 weakly similar to PIR|T06291|T06291 extensin homolog T9E... 32 0.26
TC77385 similar to PIR|T04685|T04685 hypothetical protein F4B14.... 32 0.26
TC78223 weakly similar to GP|9758310|dbj|BAB08784.1 porin-like p... 32 0.26
>TC86627 similar to PIR|T02334|T02334 probable urease accessory protein
[imported] - Arabidopsis thaliana, partial (93%)
Length = 1246
Score = 566 bits (1459), Expect = e-162
Identities = 274/274 (100%), Positives = 274/274 (100%)
Frame = +3
Query: 1 MASGGDHTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPIYSPGFFSRRA 60
MASGGDHTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPIYSPGFFSRRA
Sbjct: 51 MASGGDHTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPIYSPGFFSRRA 230
Query: 61 QPLINRDFNERAFTVGIGGPVGTGKTALMLALCQNLRDRYSLAAVTNDIFTKEDGEFLVK 120
QPLINRDFNERAFTVGIGGPVGTGKTALMLALCQNLRDRYSLAAVTNDIFTKEDGEFLVK
Sbjct: 231 QPLINRDFNERAFTVGIGGPVGTGKTALMLALCQNLRDRYSLAAVTNDIFTKEDGEFLVK 410
Query: 121 HKALPEERIRAVETGGCPHAAIREDISINLGPLEELSNLYKADLLLCESGGDNLAANFSR 180
HKALPEERIRAVETGGCPHAAIREDISINLGPLEELSNLYKADLLLCESGGDNLAANFSR
Sbjct: 411 HKALPEERIRAVETGGCPHAAIREDISINLGPLEELSNLYKADLLLCESGGDNLAANFSR 590
Query: 181 ELADYIIYIIDVSGGDKIPRKGGPGITQADLLVINKTDLAQAIGADLGVMERDALRMRDG 240
ELADYIIYIIDVSGGDKIPRKGGPGITQADLLVINKTDLAQAIGADLGVMERDALRMRDG
Sbjct: 591 ELADYIIYIIDVSGGDKIPRKGGPGITQADLLVINKTDLAQAIGADLGVMERDALRMRDG 770
Query: 241 GPFVFAQVKHNVGVEEIVNHVLQAWEAATGKKRH 274
GPFVFAQVKHNVGVEEIVNHVLQAWEAATGKKRH
Sbjct: 771 GPFVFAQVKHNVGVEEIVNHVLQAWEAATGKKRH 872
>TC79517 similar to PIR|T08874|T08874 calcium-dependent protein kinase (EC
2.7.1.-) gamma - soybean, partial (80%)
Length = 1930
Score = 39.3 bits (90), Expect = 0.002
Identities = 23/96 (23%), Positives = 44/96 (44%), Gaps = 5/96 (5%)
Frame = +3
Query: 5 GDHTHVHDHDH-HHHDHDHKHGDSFIG----ADGKVYHSHDGLAPHSHEPIYSPGFFSRR 59
G H H H+H+H H+H+H+HK+ + ++ + Y S + AP S +PI
Sbjct: 420 GIHNHNHNHNHNHNHNHNHKNHEPYVNQSKTPSQQPYQSPNKHAPRSPKPIVQ----RPN 587
Query: 60 AQPLINRDFNERAFTVGIGGPVGTGKTALMLALCQN 95
++ + F + +G +G G+ + +N
Sbjct: 588 TNAILGKQFEDVKQIYTLGKELGRGQFGVTYLCTEN 695
Score = 31.2 bits (69), Expect = 0.45
Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 8/69 (11%)
Frame = +3
Query: 6 DHTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPH-------SHEPIYSPGFFSR 58
DH HV H+H+H+H H + H+H P+ S +P SP +
Sbjct: 396 DHRHVGVSGIHNHNHNHNHNHNH-------NHNHKNHEPYVNQSKTPSQQPYQSPNKHAP 554
Query: 59 RA-QPLINR 66
R+ +P++ R
Sbjct: 555 RSPKPIVQR 581
>TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown protein
{Arabidopsis thaliana}, partial (19%)
Length = 679
Score = 35.8 bits (81), Expect = 0.018
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = -1
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H+H H HHHH H H H
Sbjct: 331 HHHLHHHHHHHHHHPHLH 278
Score = 33.5 bits (75), Expect = 0.090
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = -1
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HHHH H H H
Sbjct: 325 HLHHHHHHHHHHPHLHLH 272
Score = 28.1 bits (61), Expect = 3.8
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = -1
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HH H H H H
Sbjct: 319 HHHHHHHHHHPHLHLHLH 266
>BQ137028 similar to GP|4914334|gb|A F14N23.20 {Arabidopsis thaliana},
partial (7%)
Length = 687
Score = 35.8 bits (81), Expect = 0.018
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +2
Query: 7 HTHVHDHDHHHHDHDHKHGDS 27
H H H H HHHH H+H D+
Sbjct: 452 HNHNHHHHHHHHQEQHEHHDN 514
Score = 30.0 bits (66), Expect = 1.00
Identities = 8/14 (57%), Positives = 12/14 (85%)
Frame = +2
Query: 10 VHDHDHHHHDHDHK 23
+H+H+HHHH H H+
Sbjct: 449 LHNHNHHHHHHHHQ 490
Score = 28.5 bits (62), Expect = 2.9
Identities = 8/17 (47%), Positives = 10/17 (58%)
Frame = +2
Query: 6 DHTHVHDHDHHHHDHDH 22
+H H H H HH H+H
Sbjct: 455 NHNHHHHHHHHQEQHEH 505
>TC91060 similar to GP|18175803|gb|AAL59930.1 unknown protein {Arabidopsis
thaliana}, partial (28%)
Length = 694
Score = 35.0 bits (79), Expect = 0.031
Identities = 20/44 (45%), Positives = 22/44 (49%), Gaps = 7/44 (15%)
Frame = +3
Query: 11 HDHDHHHHDHDHKHGDSFI-------GADGKVYHSHDGLAPHSH 47
HDHDHHHH HDH H S + D K+Y GL H H
Sbjct: 258 HDHDHHHH-HDHVHQQSKLLPEELAEDEDMKLYGF--GLPHHDH 380
Score = 32.3 bits (72), Expect = 0.20
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +3
Query: 4 GGDHTHVHDHDHHHHDHDHK 23
GG+H H H HHHHDH H+
Sbjct: 249 GGEHDHDH---HHHHDHVHQ 299
Score = 30.8 bits (68), Expect = 0.58
Identities = 12/23 (52%), Positives = 15/23 (65%), Gaps = 1/23 (4%)
Frame = +1
Query: 4 GGDHTHVH-DHDHHHHDHDHKHG 25
GG+H+H H DH H HD + HG
Sbjct: 610 GGEHSHSHGDHADHDHDDNSGHG 678
Score = 28.1 bits (61), Expect = 3.8
Identities = 12/35 (34%), Positives = 16/35 (45%), Gaps = 6/35 (17%)
Frame = +3
Query: 7 HTHVHDHDHHHH------DHDHKHGDSFIGADGKV 35
H+H + HHHH DHDH H + K+
Sbjct: 207 HSHSIPNIHHHHCGGGEHDHDHHHHHDHVHQQSKL 311
>TC93162 similar to PIR|G72453|G72453 hypothetical protein APE2275 -
Aeropyrum pernix (strain K1), partial (2%)
Length = 775
Score = 34.7 bits (78), Expect = 0.040
Identities = 10/22 (45%), Positives = 15/22 (67%)
Frame = +2
Query: 3 SGGDHTHVHDHDHHHHDHDHKH 24
+ H H+ H HHH++H+HKH
Sbjct: 203 NSNQHFHILHHQHHHNNHNHKH 268
>TC90911 similar to PIR|T02681|T02681 probable zinc transporter At2g46800
[imported] - Arabidopsis thaliana, partial (45%)
Length = 671
Score = 34.3 bits (77), Expect = 0.053
Identities = 16/26 (61%), Positives = 16/26 (61%), Gaps = 2/26 (7%)
Frame = +3
Query: 4 GGDHTHVHDHDHHH-HD-HDHKHGDS 27
G DH H H HDH H HD H H HG S
Sbjct: 579 GHDHGHGHGHDHXHGHDHHGHSHGIS 656
Score = 28.9 bits (63), Expect = 2.2
Identities = 16/36 (44%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = +3
Query: 13 HDHHH-HDHDHKHGDSFIGADGKVYHSHDGLAPHSH 47
HDH H H HDH HG H H G HSH
Sbjct: 582 HDHGHGHGHDHXHG-----------HDHHG---HSH 647
>TC87570 homologue to GP|7208771|emb|CAB76908.1 putative Ruv DNA-helicase
{Cicer arietinum}, partial (36%)
Length = 711
Score = 33.9 bits (76), Expect = 0.069
Identities = 26/82 (31%), Positives = 41/82 (49%), Gaps = 1/82 (1%)
Frame = +1
Query: 77 IGGPVGTGKTALMLALCQNLRDRYSLA-AVTNDIFTKEDGEFLVKHKALPEERIRAVETG 135
+ GP GTGKTAL L +CQ L + V +++++ E + K + L E RA+
Sbjct: 427 LAGPPGTGKTALALGICQELGTKVPFCPMVGSEVYSTE----VKKTEVLMENFRRAI--- 585
Query: 136 GCPHAAIREDISINLGPLEELS 157
I+E+ + G + ELS
Sbjct: 586 ---GLRIKENKEVYEGEVTELS 642
>TC86231 similar to GP|21593313|gb|AAM65262.1 protein disulfide isomerase
precursor-like {Arabidopsis thaliana}, partial (79%)
Length = 2185
Score = 33.9 bits (76), Expect = 0.069
Identities = 36/129 (27%), Positives = 43/129 (32%), Gaps = 5/129 (3%)
Frame = +1
Query: 6 DHTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPIYSPGFFSRRAQPLIN 65
D T H HDH D G DG + G PHS E P + LI
Sbjct: 205 DTTTTH-HDHADFPDPDNFDDDDEGDDGDEFGDFSGYDPHSDEDFKEPEVDEKDVVVLIE 381
Query: 66 RDF-----NERAFTVGIGGPVGTGKTALMLALCQNLRDRYSLAAVTNDIFTKEDGEFLVK 120
R+F N + V P CQ L Y+ AA K+DG L K
Sbjct: 382 RNFTTVIENNQFVMVEFYAP--------WCGHCQALAPEYAAAATE----LKKDGVVLAK 525
Query: 121 HKALPEERI 129
A E +
Sbjct: 526 VDASVENEL 552
>TC84261 similar to GP|18650616|gb|AAL75907.1 AT4g03420/F9H3_4
{Arabidopsis thaliana}, partial (16%)
Length = 705
Score = 33.5 bits (75), Expect = 0.090
Identities = 11/19 (57%), Positives = 12/19 (62%)
Frame = +3
Query: 7 HTHVHDHDHHHHDHDHKHG 25
H H H H H HH+H H HG
Sbjct: 66 HGHEHGHGHGHHEHVHGHG 122
>TC79991 similar to GP|10177307|dbj|BAB10568. contains similarity to zinc
finger protein~gene_id:MDC12.23 {Arabidopsis thaliana},
partial (8%)
Length = 1023
Score = 33.1 bits (74), Expect = 0.12
Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 3/62 (4%)
Frame = -3
Query: 7 HTHVHDHDH--HHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPIYSPGF-FSRRAQPL 63
HTH H+H H HH+ + H H + + + H L HSH P + + QP
Sbjct: 808 HTHHHNHPHIQHHNHNQHHHHYRHHNPNHQHLYHHQVLHSHSHSNY*YPNLCYLHQHQPF 629
Query: 64 IN 65
++
Sbjct: 628 LH 623
>TC89559 similar to GP|20259581|gb|AAM14133.1 unknown protein {Arabidopsis
thaliana}, partial (55%)
Length = 1557
Score = 33.1 bits (74), Expect = 0.12
Identities = 11/19 (57%), Positives = 12/19 (62%)
Frame = -2
Query: 6 DHTHVHDHDHHHHDHDHKH 24
DH +H HDHHH H H H
Sbjct: 530 DHNVLHHHDHHHDYHFHNH 474
>TC77148 ENOD20
Length = 1108
Score = 32.7 bits (73), Expect = 0.15
Identities = 15/45 (33%), Positives = 18/45 (39%)
Frame = +1
Query: 9 HVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPIYSP 53
H H H HHHH H H G G V+ H H ++ P
Sbjct: 511 HRHHHLHHHHHHHHHLGQLQFLILGNVHQLHHHHHHHCQSRLHPP 645
Score = 28.5 bits (62), Expect = 2.9
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +1
Query: 7 HTHVHDHDHHHHDH 20
H H H H HHHH H
Sbjct: 511 HRHHHLHHHHHHHH 552
>TC86933 homologue to GP|2674201|gb|AAC61786.1| glycine-rich RNA-binding
protein {Euphorbia esula}, partial (15%)
Length = 766
Score = 32.7 bits (73), Expect = 0.15
Identities = 11/19 (57%), Positives = 13/19 (67%)
Frame = -2
Query: 6 DHTHVHDHDHHHHDHDHKH 24
DH H+H H+H HH H H H
Sbjct: 57 DHRHLHHHNHGHHLHLHIH 1
>TC80287 weakly similar to GP|12667516|gb|AAG61157.1 DBC2 {Homo sapiens},
partial (4%)
Length = 779
Score = 32.3 bits (72), Expect = 0.20
Identities = 18/49 (36%), Positives = 20/49 (40%)
Frame = +3
Query: 6 DHTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPIYSPG 54
+H H H H HHHH H H HG + H DG H Y G
Sbjct: 381 NHHHHHLHFHHHH-HHHHHG*LVLHC---TQHGDDGDFQLGHRDEYGDG 515
>TC82470 homologue to GP|23497460|gb|AAN37003.1 hypothetical protein
{Plasmodium falciparum 3D7}, partial (8%)
Length = 1064
Score = 32.3 bits (72), Expect = 0.20
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -1
Query: 9 HVHDHDHHHHDHDHKH 24
H H H HHHH H H H
Sbjct: 80 HHHHHHHHHHHHHHHH 33
Score = 32.0 bits (71), Expect = 0.26
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -1
Query: 7 HTHVHDHDHHHHDHDH 22
H H H H HHHH H H
Sbjct: 80 HHHHHHHHHHHHHHHH 33
>AJ388976 similar to PIR|E84638|E84 probable RSZp22 splicing factor
[imported] - Arabidopsis thaliana, partial (62%)
Length = 508
Score = 32.3 bits (72), Expect = 0.20
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -3
Query: 3 SGGDHTHVHDHDHHHHDHDH 22
S +H H DHDHHHH H
Sbjct: 341 SNQNHPHQTDHDHHHHHRHH 282
>TC86766 weakly similar to PIR|T06291|T06291 extensin homolog T9E8.80 -
Arabidopsis thaliana, partial (18%)
Length = 1260
Score = 32.0 bits (71), Expect = 0.26
Identities = 14/45 (31%), Positives = 16/45 (35%)
Frame = +3
Query: 7 HTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPIY 51
H H H HHHH H + + H H PH H Y
Sbjct: 366 HLITHHHPHHHHHHQYM----------PIPHRHHRYTPHPHHHQY 470
>TC77385 similar to PIR|T04685|T04685 hypothetical protein F4B14.20 -
Arabidopsis thaliana, partial (90%)
Length = 1244
Score = 32.0 bits (71), Expect = 0.26
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +3
Query: 10 VHDHDHHHHDHDHKHGDSFIGADGKVYHSH 39
+H H+H+HHD H H F K H H
Sbjct: 147 IHHHNHNHHDSIHNHTFFFYHYANKKKHEH 236
>TC78223 weakly similar to GP|9758310|dbj|BAB08784.1 porin-like protein
{Arabidopsis thaliana}, partial (82%)
Length = 1503
Score = 32.0 bits (71), Expect = 0.26
Identities = 39/146 (26%), Positives = 60/146 (40%), Gaps = 24/146 (16%)
Frame = +2
Query: 58 RRAQPLINRDFN-ERAFTVGIGGPVGTGKTALMLA----LCQNLRDRYSLAAVTNDIFTK 112
+RA+ L+ +D+N + F++ I G G TA L +L Y VT D+
Sbjct: 146 KRARDLLYKDYNFDHKFSLSIPSSTGLGLTATGLKRDKFFVGDLNTLYKSGNVTVDVKVN 325
Query: 113 EDGEFLVK---------HKA-------LPEERIRAVETGGC-PHAAIREDISINLGPLEE 155
D K H +P+ + ++ PHAAI I +N P E
Sbjct: 326 TDSNVSTKVTLNDVSLSHSKKVALSFNIPDHKSGKLDVQYLHPHAAIDSSIGLNPAPKLE 505
Query: 156 LS-NLYKADLLL-CESGGDNLAANFS 179
LS + D+ + E G D +A+FS
Sbjct: 506 LSAAIGSKDISMGAEVGFDTTSASFS 583
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.139 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,818,948
Number of Sequences: 36976
Number of extensions: 152856
Number of successful extensions: 3768
Number of sequences better than 10.0: 158
Number of HSP's better than 10.0 without gapping: 1726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2877
length of query: 274
length of database: 9,014,727
effective HSP length: 95
effective length of query: 179
effective length of database: 5,502,007
effective search space: 984859253
effective search space used: 984859253
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146651.13