BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146630.4 + phase: 0
(100 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA990835 homologue to GP|21536580|gb| unknown {Arabidopsis thali... 191 3e-50
BF519156 homologue to PIR|G85089|G850 hypothetical protein AT4g0... 38 7e-04
AW690594 similar to GP|23498163|emb hypothetical protein {Plasmo... 36 0.003
TC90628 similar to PIR|D84746|D84746 hypothetical protein At2g33... 35 0.005
BQ752101 similar to PIR|S57177|S57 branched-chain-amino-acid tra... 32 0.052
BF639598 30 0.12
TC79359 weakly similar to PIR|S49915|S49915 extensin-like protei... 29 0.26
TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Atel... 29 0.34
TC84594 similar to GP|23497092|gb|AAN36641.1 hypothetical protei... 29 0.34
TC91452 weakly similar to GP|21537012|gb|AAM61353.1 unknown {Ara... 28 0.44
TC89427 weakly similar to GP|4557063|gb|AAD22502.1| expressed pr... 28 0.57
TC92358 weakly similar to SP|Q9PTS2|DCUP_BRARE Uroporphyrinogen ... 28 0.75
TC79270 weakly similar to GP|17473774|gb|AAL38323.1 unknown prot... 28 0.75
BF635859 27 0.98
TC89394 similar to GP|14517410|gb|AAK62595.1 At1g30970/F17F8_14 ... 27 0.98
BI263077 GP|21702714|g histone H3 {Hypocrea jecorina}, complete 27 0.98
TC78014 similar to GP|17529090|gb|AAL38755.1 unknown protein {Ar... 27 1.3
TC88399 weakly similar to GP|18491217|gb|AAL69511.1 putative AMP... 27 1.3
TC80893 similar to GP|4557063|gb|AAD22502.1| expressed protein {... 27 1.3
AW683905 weakly similar to GP|9757854|dbj| contains similarity t... 27 1.3
>CA990835 homologue to GP|21536580|gb| unknown {Arabidopsis thaliana},
partial (52%)
Length = 299
Score = 191 bits (486), Expect = 3e-50
Identities = 93/93 (100%), Positives = 93/93 (100%)
Frame = +3
Query: 1 MSRRNGSGPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLLV 60
MSRRNGSGPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLLV
Sbjct: 21 MSRRNGSGPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLLV 200
Query: 61 GCPRCLMYVMLSENDPKCPKCHSTVLLDFLNAE 93
GCPRCLMYVMLSENDPKCPKCHSTVLLDFLNAE
Sbjct: 201 GCPRCLMYVMLSENDPKCPKCHSTVLLDFLNAE 299
>BF519156 homologue to PIR|G85089|G850 hypothetical protein AT4g08910
[imported] - Arabidopsis thaliana, partial (22%)
Length = 719
Score = 37.7 bits (86), Expect = 7e-04
Identities = 30/110 (27%), Positives = 49/110 (44%), Gaps = 21/110 (19%)
Frame = +2
Query: 11 LDLKLNLSPPRVNRRMESSPTRSASVSPPSS------CVSSE---------NGSSSPEAT 55
LDL+LNL+ ++ S +++ SP S C+ S NGS S +T
Sbjct: 347 LDLELNLTCESQRKKENSDQKKNSGNSPKSLSEHDDLCIESSKFKKDSGGLNGSQSWLST 526
Query: 56 S------MLLVGCPRCLMYVMLSENDPKCPKCHSTVLLDFLNAENNNNKK 99
+ M+ C +C M VM+ ++ P CP C F+++ + N K
Sbjct: 527 TEGDYKEMVATVCMQCHMLVMICKSSPACPNC------KFMHSPDQNPSK 658
>AW690594 similar to GP|23498163|emb hypothetical protein {Plasmodium
falciparum 3D7}, partial (10%)
Length = 633
Score = 35.8 bits (81), Expect = 0.003
Identities = 21/52 (40%), Positives = 30/52 (57%)
Frame = -3
Query: 5 NGSGPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATS 56
+GSG L L ++ S P + SS + S+S S SSC SS + SSS ++S
Sbjct: 283 SGSGRVLILSISSSSPSSSSSSSSSSSSSSSSSSSSSCSSSSSSSSSSSSSS 128
>TC90628 similar to PIR|D84746|D84746 hypothetical protein At2g33510
[imported] - Arabidopsis thaliana, partial (52%)
Length = 625
Score = 35.0 bits (79), Expect = 0.005
Identities = 17/38 (44%), Positives = 23/38 (59%)
Frame = +2
Query: 57 MLLVGCPRCLMYVMLSENDPKCPKCHSTVLLDFLNAEN 94
+++ GC CLMY M+ + CPKC S LL F +EN
Sbjct: 323 LVVAGCKICLMYFMVPKQVEDCPKC-SGQLLHFDRSEN 433
>BQ752101 similar to PIR|S57177|S57 branched-chain-amino-acid transaminase
(EC 2.6.1.42) BAT2 cytosolic - yeast, partial (46%)
Length = 720
Score = 31.6 bits (70), Expect = 0.052
Identities = 18/55 (32%), Positives = 25/55 (44%)
Frame = -1
Query: 2 SRRNGSGPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATS 56
SR + + P L+ SPPR R +S +S PP VSS +P +S
Sbjct: 357 SRASPATPSSPLRARSSPPRAGRSQSASTPCPSSTRPPRKAVSSRPLVPAPPPSS 193
>BF639598
Length = 588
Score = 30.4 bits (67), Expect = 0.12
Identities = 21/60 (35%), Positives = 28/60 (46%), Gaps = 8/60 (13%)
Frame = +1
Query: 5 NGSGPKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSE--------NGSSSPEATS 56
N S K+ LN SPP+ N ++ + S SVS S+ E NGS SP +S
Sbjct: 328 NPSSTKIQKNLNFSPPKDNNSTKNPLSDSGSVSSHSNSSHREKGLNPKPKNGSFSPSDSS 507
>TC79359 weakly similar to PIR|S49915|S49915 extensin-like protein - maize,
partial (3%)
Length = 1170
Score = 29.3 bits (64), Expect = 0.26
Identities = 19/39 (48%), Positives = 24/39 (60%)
Frame = +3
Query: 18 SPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATS 56
S P V+ S PT A+VSP SS V + + SS PEA+S
Sbjct: 474 STPPVSSPASSPPT--AAVSPVSSPVEAPSVSSPPEASS 584
Score = 24.3 bits (51), Expect = 8.3
Identities = 14/32 (43%), Positives = 17/32 (52%), Gaps = 1/32 (3%)
Frame = +3
Query: 28 SSPTRSASVSPPSS-CVSSENGSSSPEATSML 58
SSP S + PPSS VS N +SP + L
Sbjct: 234 SSPKSSVTAKPPSSVSVSPTNSPASPAKSPTL 329
>TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Ateline
herpesvirus 3}, partial (8%)
Length = 986
Score = 28.9 bits (63), Expect = 0.34
Identities = 17/37 (45%), Positives = 21/37 (55%)
Frame = -2
Query: 18 SPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEA 54
SPP + SS + S+S S PSS SS SS+P A
Sbjct: 679 SPPSSSSSSSSSSSSSSSWSSPSSSSSSSPFSSAPPA 569
Score = 26.9 bits (58), Expect = 1.3
Identities = 15/29 (51%), Positives = 18/29 (61%)
Frame = -2
Query: 28 SSPTRSASVSPPSSCVSSENGSSSPEATS 56
SS S+S SPPSS SS + SSS + S
Sbjct: 706 SSTPSSSSSSPPSSSSSSSSSSSSSSSWS 620
>TC84594 similar to GP|23497092|gb|AAN36641.1 hypothetical protein
{Plasmodium falciparum 3D7}, partial (0%)
Length = 791
Score = 28.9 bits (63), Expect = 0.34
Identities = 20/42 (47%), Positives = 25/42 (58%), Gaps = 3/42 (7%)
Frame = -3
Query: 25 RMESSPTR---SASVSPPSSCVSSENGSSSPEATSMLLVGCP 63
R+ SPT S +VSPPSS SS + SS E+ LL+G P
Sbjct: 522 RLLPSPTPTSLSTTVSPPSSSSSSSSSSSPSES---LLLGVP 406
>TC91452 weakly similar to GP|21537012|gb|AAM61353.1 unknown {Arabidopsis
thaliana}, partial (10%)
Length = 774
Score = 28.5 bits (62), Expect = 0.44
Identities = 15/32 (46%), Positives = 21/32 (64%)
Frame = +2
Query: 43 VSSENGSSSPEATSMLLVGCPRCLMYVMLSEN 74
V SENGSS+ E +S++ V R + + LSEN
Sbjct: 212 VESENGSSTTECSSVVTVMKGRAVRSMRLSEN 307
>TC89427 weakly similar to GP|4557063|gb|AAD22502.1| expressed protein
{Arabidopsis thaliana}, partial (50%)
Length = 753
Score = 28.1 bits (61), Expect = 0.57
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = -2
Query: 28 SSPTRSASVSPPSSCVSSENGSSSPEATSM 57
SSP +S+S+S SS SS + SSS + S+
Sbjct: 389 SSPEKSSSLSSSSSFTSSSSSSSSSSSVSL 300
Score = 24.3 bits (51), Expect = 8.3
Identities = 13/28 (46%), Positives = 18/28 (63%)
Frame = -2
Query: 28 SSPTRSASVSPPSSCVSSENGSSSPEAT 55
SS + S+S S PSS SS + SSS ++
Sbjct: 530 SSSSSSSSSSSPSSLSSSSSSSSSSSSS 447
>TC92358 weakly similar to SP|Q9PTS2|DCUP_BRARE Uroporphyrinogen
decarboxylase (EC 4.1.1.37) (URO-D) (UPD). [Zebrafish
Danio rerio], partial (58%)
Length = 824
Score = 27.7 bits (60), Expect = 0.75
Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 6/51 (11%)
Frame = +3
Query: 20 PRVNRRMESSPTRSASVSP------PSSCVSSENGSSSPEATSMLLVGCPR 64
P R ++ + A+V+P PSS +S+ GSS P ++S + CPR
Sbjct: 129 PSTTRPRATATSSPAAVTPRSPAP*PSSPLSASPGSSMPPSSSAIS*LCPR 281
>TC79270 weakly similar to GP|17473774|gb|AAL38323.1 unknown protein
{Arabidopsis thaliana}, partial (18%)
Length = 1288
Score = 27.7 bits (60), Expect = 0.75
Identities = 22/77 (28%), Positives = 31/77 (39%)
Frame = -3
Query: 17 LSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLLVGCPRCLMYVMLSENDP 76
LSP R +RR + RS + S NG+S+P R + + NDP
Sbjct: 326 LSPSRRHRRRVRNIHRSNIPLHNLTTASKSNGNSTP-----------RRIRHFT*KSNDP 180
Query: 77 KCPKCHSTVLLDFLNAE 93
KC T+ FL +
Sbjct: 179 KCTNPKLTIKRKFLQLQ 129
>BF635859
Length = 461
Score = 27.3 bits (59), Expect = 0.98
Identities = 17/51 (33%), Positives = 25/51 (48%)
Frame = -2
Query: 9 PKLDLKLNLSPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSMLL 59
P + LNL+ V+ E PTR S+S ++ VS+ P TS+ L
Sbjct: 199 PPRETSLNLT*GLVSACKEDWPTRGTSLSLTNALVSARKEDWLPRGTSLSL 47
>TC89394 similar to GP|14517410|gb|AAK62595.1 At1g30970/F17F8_14
{Arabidopsis thaliana}, partial (68%)
Length = 1627
Score = 27.3 bits (59), Expect = 0.98
Identities = 15/37 (40%), Positives = 22/37 (58%)
Frame = +2
Query: 29 SPTRSASVSPPSSCVSSENGSSSPEATSMLLVGCPRC 65
S + SA VSP S+C ++ +G S P AT ++ C C
Sbjct: 557 STSTSAMVSPASNCFNASSG-SIPTAT---IISCSEC 655
>BI263077 GP|21702714|g histone H3 {Hypocrea jecorina}, complete
Length = 682
Score = 27.3 bits (59), Expect = 0.98
Identities = 17/40 (42%), Positives = 20/40 (49%)
Frame = +3
Query: 19 PPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPEATSML 58
PP V R SSPT S S+ P S S+ + S S T L
Sbjct: 354 PPSVLSRSPSSPTSSPSLKTPISVPSTPSVSPSNPRTFSL 473
>TC78014 similar to GP|17529090|gb|AAL38755.1 unknown protein {Arabidopsis
thaliana}, partial (74%)
Length = 2305
Score = 26.9 bits (58), Expect = 1.3
Identities = 11/35 (31%), Positives = 23/35 (65%)
Frame = -1
Query: 25 RMESSPTRSASVSPPSSCVSSENGSSSPEATSMLL 59
R+ S + S+++ PP+S ++ NG++ P T++ L
Sbjct: 1849 RIVSIASASSTIPPPASFINRINGATIPALTALAL 1745
>TC88399 weakly similar to GP|18491217|gb|AAL69511.1 putative AMP-binding
protein {Arabidopsis thaliana}, partial (62%)
Length = 1286
Score = 26.9 bits (58), Expect = 1.3
Identities = 17/36 (47%), Positives = 22/36 (60%)
Frame = +1
Query: 18 SPPRVNRRMESSPTRSASVSPPSSCVSSENGSSSPE 53
SPP V+ SSP+ S SPP C++S + S SPE
Sbjct: 163 SPP*VSAAATSSPS-SLPTSPP--CMNSTSPSPSPE 261
>TC80893 similar to GP|4557063|gb|AAD22502.1| expressed protein {Arabidopsis
thaliana}, partial (39%)
Length = 883
Score = 26.9 bits (58), Expect = 1.3
Identities = 16/32 (50%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = -1
Query: 28 SSPT-RSASVSPPSSCVSSENGSSSPEATSML 58
SSP S+S S SSC SS + SSS + S+L
Sbjct: 406 SSPE*SSSSPSSSSSCTSSSSSSSSSSSVSLL 311
Score = 24.6 bits (52), Expect = 6.3
Identities = 14/30 (46%), Positives = 18/30 (59%)
Frame = -1
Query: 28 SSPTRSASVSPPSSCVSSENGSSSPEATSM 57
SS + S+S S PSS SS SSS +S+
Sbjct: 550 SSSSSSSSSSSPSSSSSSSPSSSSSSPSSL 461
>AW683905 weakly similar to GP|9757854|dbj| contains similarity to apoptosis
antagonizing transcription factor~gene_id:MFB13.10,
partial (12%)
Length = 492
Score = 26.9 bits (58), Expect = 1.3
Identities = 14/28 (50%), Positives = 20/28 (71%)
Frame = -3
Query: 28 SSPTRSASVSPPSSCVSSENGSSSPEAT 55
SS + S+S+ P SSC SS + SSSP ++
Sbjct: 289 SSCSISSSLHPFSSCPSSSSPSSSPSSS 206
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.125 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,645,980
Number of Sequences: 36976
Number of extensions: 53667
Number of successful extensions: 452
Number of sequences better than 10.0: 90
Number of HSP's better than 10.0 without gapping: 426
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 445
length of query: 100
length of database: 9,014,727
effective HSP length: 76
effective length of query: 24
effective length of database: 6,204,551
effective search space: 148909224
effective search space used: 148909224
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 50 (23.9 bits)
Medicago: description of AC146630.4